Restriction map for
pFN21AF0316
(Circular) (Six-base) MAPSORT of: pFN21AF0316.seq Check: 603 from: 1 to: 6625
pFN21AF0316 6625 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4435 278
Size: 53 83 186 936 2899 2468
Fragments arranged by size:
2899 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3968 1937
Size: 2031 4594
Acc65I G'GTAC_C
Cuts at: 3946 3946
Size: 6625
AclI AA'CG_TT
Cuts at: 1563 4752 5125 1563
Size: 3189 373 3063
Fragments arranged by size:
3189 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3728 4688 6072 6340 1632
Size: 2096 960 1384 268 1917
Fragments arranged by size:
2096 1917 1384 960 268
AflII C'TTAA_G
Cuts at: 820 1017 3278 3728 820
Size: 197 2261 450 3717
Fragments arranged by size:
3717 2261 450 197
AflIII A'CryG_T
Cuts at: 1203 5524 6501 1203
Size: 4321 977 1327
Fragments arranged by size:
4321 1327 977
AgeI A'CCGG_T
Cuts at: 5456 5456
Size: 6625
AhdI GACnn_n'nnGTC
Cuts at: 5354 5354
Size: 6625
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6625
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2533 1184
Size: 1349 5276
AlwNI CAG_nnn'CTG
Cuts at: 2057 2464 3026 3073 3103 3559 4368 5940 2057
Size: 407 562 47 30 456 809 1572 2742
Fragments arranged by size:
2742 1572 809 562 456 407 47 30
ApaLI G'TGCA_C
Cuts at: 4682 5838 4682
Size: 1156 5469
ApoI r'AATT_y
Cuts at: 1493 2119 2688 2695 3182 3321 3737 3934 4160
Size: 626 569 7 487 139 416 197 226
Cuts at: 4160 4312 6421 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 626 569 487 416 226 197
152 139 7
AseI AT'TA_AT
Cuts at: 160 1032 3774 5179 160
Size: 872 2742 1405 1606
Fragments arranged by size:
2742 1606 1405 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6625
AvaI C'yCGr_G
Cuts at: 1870 1943 3950 1870
Size: 73 2007 4545
Fragments arranged by size:
4545 2007 73
BamHI G'GATC_C
Cuts at: 3955 4322 3955
Size: 367 6258
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3946 5401 618
Size: 325 217 627 2159 1455 1842
Fragments arranged by size:
2159 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2255 3944 1391
Size: 864 1689 4072
Fragments arranged by size:
4072 1689 864
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3139 928
Size: 971 1240 4414
Fragments arranged by size:
4414 1240 971
BbvCI CC'TCA_GC
Cuts at: 2538 2538
Size: 6625
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4816 4850 1903
Size: 34 2879 34 3678
Fragments arranged by size:
3678 2879 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4517 5727 4517
Size: 1210 5415
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6625
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5236 136
Size: 107 122 71 631 605 3564 1525
Fragments arranged by size:
3564 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6620 6620
Size: 6625
BlpI GC'TnA_GC
Cuts at: 3273 4042 3273
Size: 769 5856
Bme1580I G_kGCm'C
Cuts at: 1790 2037 2301 3399 4686 5842 1790
Size: 247 264 1098 1287 1156 2573
Fragments arranged by size:
2573 1287 1156 1098 264 247
BmgBI CAC'GTC
Cuts at: 1318 3112 1318
Size: 1794 4831
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4356 5314 442
Size: 416 53 83 399 24 2939 958 1753
Fragments arranged by size:
2939 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2495 3849 1056
Size: 1439 1354 3832
Fragments arranged by size:
3832 1439 1354
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2486 2530 2624 2669 3002 3080 3357 3413
Size: 1247 44 94 45 333 78 277 56
Cuts at: 3413 3519 5285 1239
Size: 106 1766 2579
Fragments arranged by size:
2579 1766 1247 333 277 106 94 78
56 45 44
Bpu10I CC'TnA_GC
Cuts at: 2538 2538
Size: 6625
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3032 3588 3623 4750 5615 5913 3032
Size: 556 35 1127 865 298 3744
Fragments arranged by size:
3744 1127 865 556 298 35
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5288 882
Size: 617 3789 2219
Fragments arranged by size:
3789 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3920 4416 493
Size: 711 2716 496 2702
Fragments arranged by size:
2716 2702 711 496
BsaBI GATnn'nnATC
Cuts at: 6619 6619
Size: 6625
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4432 4814 5449 275
Size: 53 83 186 936 2899 382 635 1451
Fragments arranged by size:
2899 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5058 5456 5730 5877 6288 5058
Size: 398 274 147 411 5395
Fragments arranged by size:
5395 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2597 2627 3063 3093 3216
Size: 30 165 30 1194 30 436 30 123
Cuts at: 3216 3246 1178
Size: 30 4557
Fragments arranged by size:
4557 1194 436 165 123 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6625
BseYI C'CCAG_C
Cuts at: 2923 3006 3019 3066 5828 6324 2923
Size: 83 13 47 2762 496 3224
Fragments arranged by size:
3224 2762 496 83 47 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2282 2459 3050 1163
Size: 573 546 177 591 4738
Fragments arranged by size:
4738 591 573 546 177
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4075 4836 5864 1540
Size: 275 186 2074 761 1028 2301
Fragments arranged by size:
2301 2074 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2447 3159 3297 4686 4771 5842 2447
Size: 712 138 1389 85 1071 3230
Fragments arranged by size:
3230 1389 1071 712 138 85
BsmI GAATG_Cn'
Cuts at: 2659 2992 4136 4229 2659
Size: 333 1144 93 5055
Fragments arranged by size:
5055 1144 333 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2037 2255 2301 2447 3159 3297 3399
Size: 399 247 218 46 146 712 138 102
Cuts at: 3399 3944 4686 4771 5842 1391
Size: 545 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 712 545 399 247 218
146 138 102 85 46
BspEI T'CCGG_A
Cuts at: 6288 6288
Size: 6625
BspHI T'CATG_A
Cuts at: 2067 2211 4514 6244 2067
Size: 144 2303 1730 2448
Fragments arranged by size:
2448 2303 1730 144
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3980 844
Size: 3136 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3523 4512 1394
Size: 429 1700 989 3507
Fragments arranged by size:
3507 1700 989 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2242 3138 5120 5294 65
Size: 1183 994 896 1982 174 1396
Fragments arranged by size:
1982 1396 1183 994 896 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2760 5269 5456 1700
Size: 110 950 2509 187 2869
Fragments arranged by size:
2869 2509 950 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6625
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6625
BssSI C'ACGA_G
Cuts at: 2386 4685 5697 2386
Size: 2299 1012 3314
Fragments arranged by size:
3314 2299 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6625
BstBI TT'CG_AA
Cuts at: 5429 5429
Size: 6625
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6625
BstXI CCAn_nnnn'nTGG
Cuts at: 2071 2071
Size: 6625
BstYI r'GATC_y
Cuts at: 1972 2391 3831 3955 4322 4707 4724 6176 6262
Size: 419 1440 124 367 385 17 1452 86
Cuts at: 6262 6274 6620 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1440 419 385 367 346 124
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2160 2256 513
Size: 552 939 156 96 4882
Fragments arranged by size:
4882 939 552 156 96
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2370 487
Size: 683 56 1144 4742
Fragments arranged by size:
4742 1144 683 56
BtsI GCAGTG_nn'
Cuts at: 2037 2997 3492 4141 4935 4955 2037
Size: 960 495 649 794 20 3707
Fragments arranged by size:
3707 960 794 649 495 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6625
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6590
DraI TTT'AAA
Cuts at: 2787 3929 2787
Size: 1142 5483
DraIII CAC_nnn'GTG
Cuts at: 2482 2482
Size: 6625
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5632 809
Size: 507 4316 1802
Fragments arranged by size:
4316 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2172 4072 4961
Size: 54 1425 50 275 18 342 1900 889
Cuts at: 4961 6590 8
Size: 1629 43
Fragments arranged by size:
1900 1629 1425 889 342 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4072 1537
Size: 275 2260 4090
Fragments arranged by size:
4090 2260 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2025 2097 3240 4555 1353
Size: 672 72 1143 1315 3423
Fragments arranged by size:
3423 1315 1143 672 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5212 5586 5732 387
Size: 1064 138 3623 374 146 1280
Fragments arranged by size:
3623 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2486 2530 2624 2669 3002 3080 3357
Size: 393 854 44 94 45 333 78 277
Cuts at: 3357 3413 3519 3728 4688 5285 6072 6340 1239
Size: 56 106 209 960 597 787 268 1524
Fragments arranged by size:
1524 960 854 787 597 393 333 277
268 209 106 94 78 56 45 44
EcoNI CCTnn'n_nnAGG
Cuts at: 2894 3127 2894
Size: 233 6392
EcoO109I rG'GnC_Cy
Cuts at: 2347 2461 3424 2347
Size: 114 963 5548
Fragments arranged by size:
5548 963 114
EcoRI G'AATT_C
Cuts at: 2119 3182 3934 4312 2119
Size: 1063 752 378 4432
Fragments arranged by size:
4432 1063 752 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3244 3743 3244
Size: 499 6126
FspI TGC'GCA
Cuts at: 5131 5131
Size: 6625
HaeII r_GCGC'y
Cuts at: 1678 5772 1678
Size: 4094 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2703 2735 2886 2918 3333
Size: 32 135 32 1131 32 151 32 415
Cuts at: 3333 3365 3586 3618 3850 3882 3892 3924 5266
Size: 32 221 32 232 32 10 32 1342
Cuts at: 5266 5298 5340 5372 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1342 1131 415 232 221 151 135
42 32 32 32 32 32 32 32
32 32 32 10
HincII GTy'rAC
Cuts at: 669 1657 1938 3969 4215 669
Size: 988 281 2031 246 3079
Fragments arranged by size:
3079 2031 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3194 3613 748
Size: 2446 419 3760
Fragments arranged by size:
3760 2446 419
HpaI GTT'AAC
Cuts at: 4215 4215
Size: 6625
KpnI G_GTAC'C
Cuts at: 3950 3950
Size: 6625
MfeI C'AATT_G
Cuts at: 4224 4224
Size: 6625
MluI A'CGCG_T
Cuts at: 6501 6501
Size: 6625
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5739 5923 1305
Size: 4434 184 2007
Fragments arranged by size:
4434 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2174 10
Size: 54 1425 343 342 4461
Fragments arranged by size:
4461 1425 343 342 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2069 2285 4583 4942 5101 518
Size: 648 903 216 2298 359 159 2042
Fragments arranged by size:
2298 2042 903 648 359 216 159
MspA1I CmG'CkG
Cuts at: 1606 2110 2473 3023 3518 3542 4041 4718 5866
Size: 504 363 550 495 24 499 677 1148
Cuts at: 5866 6111 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 550 504 499 495 363
245 24
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5134
Fragments arranged by size:
5134 939 552
NdeI CA'TA_TG
Cuts at: 387 2793 387
Size: 2406 4219
NheI G'CTAG_C
Cuts at: 1052 2491 3845 1052
Size: 1439 1354 3832
Fragments arranged by size:
3832 1439 1354
NotI GC'GGCC_GC
Cuts at: 4072 4072
Size: 6625
NspI r_CATG'y
Cuts at: 1132 1229 2707 3119 3983 5528 1132
Size: 97 1478 412 864 1545 2229
Fragments arranged by size:
2229 1545 1478 864 412 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6625
PciI A'CATG_T
Cuts at: 5524 5524
Size: 6625
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2326 2464 3061 1108
Size: 1218 138 597 4672
Fragments arranged by size:
4672 1218 597 138
PfoI T'CCnGG_A
Cuts at: 3096 3334 3618 3096
Size: 238 284 6103
Fragments arranged by size:
6103 284 238
PmeI GTTT'AAAC
Cuts at: 3929 3929
Size: 6625
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4694 4726 6207 6239 4694
Size: 32 1481 32 5080
Fragments arranged by size:
5080 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3424 3424
Size: 6625
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6625
PsiI TTA'TAA
Cuts at: 4195 4195
Size: 6625
PstI C_TGCA'G
Cuts at: 830 2032 3240 3444 3477 3977 830
Size: 1202 1208 204 33 500 3478
Fragments arranged by size:
3478 1208 1202 500 204 33
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6625
PvuII CAG'CTG
Cuts at: 2110 2473 3542 2110
Size: 363 1069 5193
Fragments arranged by size:
5193 1069 363
SalI G'TCGA_C
Cuts at: 1936 3967 1936
Size: 2031 4594
SapI GCTCTTCn'nnn_
Cuts at: 2097 2097
Size: 6625
SbfI CC_TGCA'GG
Cuts at: 3977 3977
Size: 6625
ScaI AGT'ACT
Cuts at: 4873 6585 4873
Size: 1712 4913
SexAI A'CCwGG_T
Cuts at: 2041 2041
Size: 6625
SfcI C'TryA_G
Cuts at: 826 1045 2028 3049 3236 3440 3473 3973 5108
Size: 219 983 1021 187 204 33 500 1135
Cuts at: 5108 5789 5980 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1021 983 681 500 219 204
191 187 33
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6625
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6625
SmaI CCC'GGG
Cuts at: 1872 3952 1872
Size: 2080 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3011 3278 3603 3638 3728 4729
Size: 197 926 1068 267 325 35 90 1001
Cuts at: 4729 5630 5892 820
Size: 901 262 1553
Fragments arranged by size:
1553 1068 1001 926 901 325 267 262
197 90 35
SnaBI TAC'GTA
Cuts at: 493 4416 493
Size: 3923 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6625
SphI G_CATG'C
Cuts at: 1132 3119 3983 1132
Size: 1987 864 3774
Fragments arranged by size:
3774 1987 864
SspI AAT'ATT
Cuts at: 5 52 2783 3263 4549 5
Size: 47 2731 480 1286 2081
Fragments arranged by size:
2731 2081 1286 480 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6625
StyI C'CwwG_G
Cuts at: 513 1065 2004 4064 513
Size: 552 939 2060 3074
Fragments arranged by size:
3074 2060 939 552
SwaI ATTT'AAAT
Cuts at: 2787 2787
Size: 6625
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 1997 2885 4652 4669 4822 5007 5452
Size: 1255 417 888 1767 17 153 185 445
Cuts at: 5452 325
Size: 1498
Fragments arranged by size:
1767 1498 1255 888 445 417 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4871 6583 96
Size: 274 80 33 51 1443 2894 1712 138
Fragments arranged by size:
2894 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2278 2310 3216 3248 6585 6617 2278
Size: 32 906 32 3337 32 2286
Fragments arranged by size:
3337 2286 906 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6625
XbaI T'CTAG_A
Cuts at: 3961 3961
Size: 6625
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2086 2577 2086
Size: 491 6134
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6625
XmaI C'CCGG_G
Cuts at: 1870 3950 1870
Size: 2080 4545
XmnI GAAnn'nnTTC
Cuts at: 3669 3738 4754 3669
Size: 69 1016 5540
Fragments arranged by size:
5540 1016 69
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4433 276
Size: 53 83 186 936 2899 2468
Fragments arranged by size:
2899 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI SwaI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SfiI SfoI SrfI