Restriction map for
pFN21AF0267
(Circular) (Six-base) MAPSORT of: pFN21AF0267.seq Check: 9930 from: 1 to: 8008
pFN21AF0267 8008 bp
With 182 enzymes: SgfI *
January 16, 2009 15:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2088 2769 4894 2088
Size: 681 2125 5202
Fragments arranged by size:
5202 2125 681
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5818 278
Size: 53 83 186 936 4282 2468
Fragments arranged by size:
4282 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4229 5351 1937
Size: 2292 1122 4594
Fragments arranged by size:
4594 2292 1122
Acc65I G'GTAC_C
Cuts at: 4422 5329 4422
Size: 907 7101
AclI AA'CG_TT
Cuts at: 1563 3315 6135 6508 1563
Size: 1752 2820 373 3063
Fragments arranged by size:
3063 2820 1752 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3819 4506 4611 5187 6071 7455 7723 1632
Size: 2187 687 105 576 884 1384 268 1917
Fragments arranged by size:
2187 1917 1384 884 687 576 268 105
AfeI AGC'GCT
Cuts at: 2381 2381
Size: 8008
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7811
AflIII A'CryG_T
Cuts at: 1203 2632 6907 7884 1203
Size: 1429 4275 977 1327
Fragments arranged by size:
4275 1429 1327 977
AgeI A'CCGG_T
Cuts at: 6839 6839
Size: 8008
AhdI GACnn_n'nnGTC
Cuts at: 3508 6737 3508
Size: 3229 4779
AleI CACnn'nnGTG
Cuts at: 1166 2633 1166
Size: 1467 6541
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4912 1184
Size: 3728 4280
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2403 2435 2403
Size: 32 7976
AlwNI CAG_nnn'CTG
Cuts at: 3566 3768 3937 4484 5751 7323 3566
Size: 202 169 547 1267 1572 4251
Fragments arranged by size:
4251 1572 1267 547 202 169
ApaI G_GGCC'C
Cuts at: 3045 4324 3045
Size: 1279 6729
ApaLI G'TGCA_C
Cuts at: 6065 7221 6065
Size: 1156 6852
ApoI r'AATT_y
Cuts at: 1493 2129 2210 5317 5543 5695 7804 1493
Size: 636 81 3107 226 152 2109 1697
Fragments arranged by size:
3107 2109 1697 636 226 152 81
AseI AT'TA_AT
Cuts at: 160 1032 6562 160
Size: 872 5530 1606
Fragments arranged by size:
5530 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8008
AvaI C'yCGr_G
Cuts at: 1870 1943 2709 2726 4900 4950 5016 5333 1870
Size: 73 766 17 2174 50 66 317 4545
Fragments arranged by size:
4545 2174 766 317 73 66 50 17
BamHI G'GATC_C
Cuts at: 2311 5186 5338 5705 2311
Size: 2875 152 367 4614
Fragments arranged by size:
4614 2875 367 152
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2101 4422 4822 4882 5329
Size: 325 217 627 314 2321 400 60 447
Cuts at: 5329 6784 618
Size: 1455 1842
Fragments arranged by size:
2321 1842 1455 627 447 400 325 314
217 60
BanII G_rGCy'C
Cuts at: 1391 2527 3045 3672 4324 5160 5327 1391
Size: 1136 518 627 652 836 167 4072
Fragments arranged by size:
4072 1136 836 652 627 518 167
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4464 928
Size: 971 2565 4472
Fragments arranged by size:
4472 2565 971
BbvCI CC'TCA_GC
Cuts at: 2349 2537 2934 4341 4377 4539 4683 2349
Size: 188 397 1407 36 162 144 5674
Fragments arranged by size:
5674 1407 397 188 162 144 36
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6199 6233 1903
Size: 34 4262 34 3678
Fragments arranged by size:
4262 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5900 7110 5900
Size: 1210 6798
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8008
BfrBI ATG'CAT
Cuts at: 2497 5267 2497
Size: 2770 5238
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3031 3194 4708
Size: 107 122 71 631 605 1359 163 1514
Cuts at: 4708 4758 4912 6619 136
Size: 50 154 1707 1525
Fragments arranged by size:
1707 1525 1514 1359 631 605 163 154
122 107 71 50
BglII A'GATC_T
Cuts at: 8003 8003
Size: 8008
BlpI GC'TnA_GC
Cuts at: 3993 5425 3993
Size: 1432 6576
Bme1580I G_kGCm'C
Cuts at: 1790 2106 2485 3045 3297 3353 4324 4827 6069
Size: 316 379 560 252 56 971 503 1242
Cuts at: 6069 7225 1790
Size: 1156 2573
Fragments arranged by size:
2573 1242 1156 971 560 503 379 316
252 56
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8008
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3149 4710 4848
Size: 416 53 83 399 24 1732 1561 138
Cuts at: 4848 5739 6697 442
Size: 891 958 1753
Fragments arranged by size:
1753 1732 1561 958 891 416 399 138
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8008
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3718 3718
Size: 8008
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2459 3518 3683 3923 4020 4578 4634 4950
Size: 1220 1059 165 240 97 558 56 316
Cuts at: 4950 5174 6668 1239
Size: 224 1494 2579
Fragments arranged by size:
2579 1494 1220 1059 558 316 240 224
165 97 56
Bpu10I CC'TnA_GC
Cuts at: 2349 2376 2537 2934 4341 4377 4539 4683 5144
Size: 27 161 397 1407 36 162 144 461
Cuts at: 5144 2349
Size: 5213
Fragments arranged by size:
5213 1407 461 397 162 161 144 36
27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3365 3436 3535 5025 6133 6998 7296 3365
Size: 71 99 1490 1108 865 298 4077
Fragments arranged by size:
4077 1490 1108 865 298 99 71
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2185 4037 4539 6671 882
Size: 617 686 1852 502 2132 2219
Fragments arranged by size:
2219 2132 1852 686 617 502
BsaAI yAC'GTr
Cuts at: 493 1204 2635 5799 493
Size: 711 1431 3164 2702
Fragments arranged by size:
3164 2702 1431 711
BsaBI GATnn'nnATC
Cuts at: 8002 8002
Size: 8008
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5815 6197 6832 275
Size: 53 83 186 936 4282 382 635 1451
Fragments arranged by size:
4282 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6441 6839 7113 7260 7671 6441
Size: 398 274 147 411 6778
Fragments arranged by size:
6778 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2258 2288 2672 2702 3518
Size: 30 165 30 855 30 384 30 816
Cuts at: 3518 3548 4406 4436 1178
Size: 30 858 30 4750
Fragments arranged by size:
4750 858 855 816 384 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2153 2173 2266 3680 3710 3821 4233 4358
Size: 967 20 93 1414 30 111 412 125
Cuts at: 4358 4506 4706 5124 1186
Size: 148 200 418 4070
Fragments arranged by size:
4070 1414 967 418 412 200 148 125
111 93 30 20
BseYI C'CCAG_C
Cuts at: 3008 3044 3176 3197 3761 3966 4088 7211 7707
Size: 36 132 21 564 205 122 3123 496
Cuts at: 7707 3008
Size: 3309
Fragments arranged by size:
3309 3123 564 496 205 132 122 36
21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2145 3234 3542 5193 1163
Size: 573 409 1089 308 1651 3978
Fragments arranged by size:
3978 1651 1089 573 409 308
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5458 6219 7247 1540
Size: 275 186 3457 761 1028 2301
Fragments arranged by size:
3457 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2079 2838 3672 5160 6069 6154 7225 2079
Size: 759 834 1488 909 85 1071 2862
Fragments arranged by size:
2862 1488 1071 909 834 759 85
BsmI GAATG_Cn'
Cuts at: 5519 5612 5519
Size: 93 7915
BsmBI CGTCTCn'nnnn_
Cuts at: 2278 2278
Size: 8008
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2079 2106 2485 2527 2838 3045 3297
Size: 399 289 27 379 42 311 207 252
Cuts at: 3297 3353 3672 4324 4827 5160 5327 6069 6154
Size: 56 319 652 503 333 167 742 85
Cuts at: 6154 7225 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 652 503 399 379 333
319 311 289 252 207 167 85 56
42 27
BspEI T'CCGG_A
Cuts at: 7671 7671
Size: 8008
BspHI T'CATG_A
Cuts at: 2448 5897 7627 2448
Size: 3449 1730 2829
Fragments arranged by size:
3449 2829 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2088 2244 2541 2769 4894 5247 5363 844
Size: 1244 156 297 228 2125 353 116 3489
Fragments arranged by size:
3489 2125 1244 353 297 228 156 116
BsrBI CCG'CTC
Cuts at: 1394 1823 2050 2224 4809 5895 1394
Size: 429 227 174 2585 1086 3507
Fragments arranged by size:
3507 2585 1086 429 227 174
BsrDI GCAATG_nn'
Cuts at: 65 1248 3065 4866 6503 6677 65
Size: 1183 1817 1801 1637 174 1396
Fragments arranged by size:
1817 1801 1637 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2328 3366 4911 6652 6839 1700
Size: 110 518 1038 1545 1741 187 2869
Fragments arranged by size:
2869 1741 1545 1038 518 187 110
BsrGI T'GTAC_A
Cuts at: 96 4360 96
Size: 4264 3744
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8008
BssSI C'ACGA_G
Cuts at: 3171 6068 7080 3171
Size: 2897 1012 4099
Fragments arranged by size:
4099 2897 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8008
BstBI TT'CG_AA
Cuts at: 6812 6812
Size: 8008
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8008
BstXI CCAn_nnnn'nTGG
Cuts at: 2614 2648 2986 4451 4556 2614
Size: 34 338 1465 105 6066
Fragments arranged by size:
6066 1465 338 105 34
BstYI r'GATC_y
Cuts at: 1972 2160 2311 2391 5186 5338 5705 6090 6107
Size: 188 151 80 2795 152 367 385 17
Cuts at: 6107 7559 7645 7657 8003 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2795 1977 1452 385 367 346 188 152
151 86 80 17 12
BstZ17I GTA'TAC
Cuts at: 4230 4230
Size: 8008
Bsu36I CC'TnA_GG
Cuts at: 2560 3126 3687 4704 2560
Size: 566 561 1017 5864
Fragments arranged by size:
5864 1017 566 561
BtgI C'CryG_G
Cuts at: 513 1065 2924 3337 3774 513
Size: 552 1859 413 437 4747
Fragments arranged by size:
4747 1859 552 437 413
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2799 487
Size: 683 56 1573 5696
Fragments arranged by size:
5696 1573 683 56
BtsI GCAGTG_nn'
Cuts at: 2266 2747 2966 5524 6318 6338 2266
Size: 481 219 2558 794 20 3936
Fragments arranged by size:
3936 2558 794 481 219 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8008
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7973
DraI TTT'AAA
Cuts at: 5312 5312
Size: 8008
DraIII CAC_nnn'GTG
Cuts at: 2999 3143 2999
Size: 144 7864
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7015 809
Size: 507 5699 1802
Fragments arranged by size:
5699 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2511 2899 2972
Size: 54 1425 50 275 18 681 388 73
Cuts at: 2972 3056 3324 3368 4909 5455 6344 7973 8
Size: 84 268 44 1541 546 889 1629 43
Fragments arranged by size:
1629 1541 1425 889 681 546 388 275
268 84 73 54 50 44 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5455 1537
Size: 275 3643 4090
Fragments arranged by size:
4090 3643 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3948 4017 4131 4149 4257 4569 4654 4656
Size: 2595 69 114 18 108 312 85 2
Cuts at: 4656 5938 1353
Size: 1282 3423
Fragments arranged by size:
3423 2595 1282 312 114 108 85 69
18 2
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6595 6969 7115 387
Size: 1064 138 5006 374 146 1280
Fragments arranged by size:
5006 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3670 5158 3670
Size: 1488 6520
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2459 3518 3683 3819 3923 4020 4506
Size: 393 827 1059 165 136 104 97 486
Cuts at: 4506 4578 4611 4634 4950 5174 5187 6071 6668
Size: 72 33 23 316 224 13 884 597
Cuts at: 6668 7455 7723 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1059 884 827 787 597 486 393
316 268 224 165 136 104 97 72
33 23 13
EcoNI CCTnn'n_nnAGG
Cuts at: 2285 3325 4969 5292 2285
Size: 1040 1644 323 5001
Fragments arranged by size:
5001 1644 1040 323
EcoO109I rG'GnC_Cy
Cuts at: 2433 2706 2731 3041 3866 4306 4320 4947 2433
Size: 273 25 310 825 440 14 627 5494
Fragments arranged by size:
5494 825 627 440 310 273 25 14
EcoRI G'AATT_C
Cuts at: 5317 5695 5317
Size: 378 7630
FspI TGC'GCA
Cuts at: 6514 6514
Size: 8008
HaeII r_GCGC'y
Cuts at: 1678 2383 7155 1678
Size: 705 4772 2531
Fragments arranged by size:
4772 2531 705
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2936 2968 3494 3518 3526
Size: 32 135 32 1364 32 526 24 8
Cuts at: 3526 3550 3713 3745 4571 4603 5023 5055 5147
Size: 24 163 32 826 32 420 32 92
Cuts at: 5147 5179 6649 6681 6723 6755 1373
Size: 32 1470 32 42 32 2626
Fragments arranged by size:
2626 1470 1364 826 526 420 163 135
92 42 32 32 32 32 32 32
32 32 32 24 24 8
HincII GTy'rAC
Cuts at: 669 1657 1938 3259 4621 5352 5598 669
Size: 988 281 1321 1362 731 246 3079
Fragments arranged by size:
3079 1362 1321 988 731 281 246
HindIII A'AGCT_T
Cuts at: 748 2198 748
Size: 1450 6558
HpaI GTT'AAC
Cuts at: 5598 5598
Size: 8008
KpnI G_GTAC'C
Cuts at: 4426 5333 4426
Size: 907 7101
MfeI C'AATT_G
Cuts at: 2366 5607 2366
Size: 3241 4767
MluI A'CGCG_T
Cuts at: 7884 7884
Size: 8008
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2211 2979 3414 4822 7122 7306 1305
Size: 906 768 435 1408 2300 184 2007
Fragments arranged by size:
2300 2007 1408 906 768 435 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2513 2901 3326 10
Size: 54 1425 343 681 388 425 4692
Fragments arranged by size:
4692 1425 681 425 388 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2591 2612 2633 5966 6325 6484 518
Size: 648 1425 21 21 3333 359 159 2042
Fragments arranged by size:
3333 2042 1425 648 359 159 21 21
MspA1I CmG'CkG
Cuts at: 1606 2579 3008 3765 3785 5424 6101 7249 7494
Size: 973 429 757 20 1639 677 1148 245
Cuts at: 7494 1606
Size: 2120
Fragments arranged by size:
2120 1639 1148 973 757 677 429 245
20
NaeI GCC'GGC
Cuts at: 4913 4913
Size: 8008
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7456
NdeI CA'TA_TG
Cuts at: 387 5263 387
Size: 4876 3132
NgoMIV G'CCGG_C
Cuts at: 4911 4911
Size: 8008
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8008
NotI GC'GGCC_GC
Cuts at: 5455 5455
Size: 8008
NsiI A_TGCA'T
Cuts at: 2499 5269 2499
Size: 2770 5238
NspI r_CATG'y
Cuts at: 1132 1229 2497 2501 3966 4771 5366 6911 1132
Size: 97 1268 4 1465 805 595 1545 2229
Fragments arranged by size:
2229 1545 1465 1268 805 595 97 4
PasI CC'CwG_GG
Cuts at: 1348 3632 3647 5288 1348
Size: 2284 15 1641 4068
Fragments arranged by size:
4068 2284 1641 15
PciI A'CATG_T
Cuts at: 6907 6907
Size: 8008
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2520 3176 1108
Size: 1412 656 5940
Fragments arranged by size:
5940 1412 656
PfoI T'CCnGG_A
Cuts at: 4927 5189 4927
Size: 262 7746
PmeI GTTT'AAAC
Cuts at: 5312 5312
Size: 8008
PmlI CAC'GTG
Cuts at: 2635 2635
Size: 8008
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3293 3325 6077 6109 7590 7622 3293
Size: 32 2752 32 1481 32 3679
Fragments arranged by size:
3679 2752 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2433 2706 2731 3866 4306 4947 2433
Size: 273 25 1135 440 641 5494
Fragments arranged by size:
5494 1135 641 440 273 25
PshAI GACnn'nnGTC
Cuts at: 1613 2238 3852 4109 4728 1613
Size: 625 1614 257 619 4893
Fragments arranged by size:
4893 1614 625 619 257
PsiI TTA'TAA
Cuts at: 5578 5578
Size: 8008
PspOMI G'GGCC_C
Cuts at: 3041 4320 3041
Size: 1279 6729
PstI C_TGCA'G
Cuts at: 830 2085 2778 3007 5360 830
Size: 1255 693 229 2353 3478
Fragments arranged by size:
3478 2353 1255 693 229
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8008
PvuII CAG'CTG
Cuts at: 3008 3765 3008
Size: 757 7251
SacI G_AGCT'C
Cuts at: 3672 5160 3672
Size: 1488 6520
SalI G'TCGA_C
Cuts at: 1936 5350 1936
Size: 3414 4594
SanDI GG'GwC_CC
Cuts at: 2706 4947 2706
Size: 2241 5767
SapI GCTCTTCn'nnn_
Cuts at: 3948 3948
Size: 8008
SbfI CC_TGCA'GG
Cuts at: 5360 5360
Size: 8008
ScaI AGT'ACT
Cuts at: 6256 7968 6256
Size: 1712 6296
SfcI C'TryA_G
Cuts at: 826 1045 2081 2355 2428 2774 3003 3096 3359
Size: 219 1036 274 73 346 229 93 263
Cuts at: 3359 5356 6491 7172 7363 826
Size: 1997 1135 681 191 1471
Fragments arranged by size:
1997 1471 1135 1036 681 346 274 263
229 219 191 93 73
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8008
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8008
SmaI CCC'GGG
Cuts at: 1872 2711 5018 5335 1872
Size: 839 2307 317 4545
Fragments arranged by size:
4545 2307 839 317
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3344 3451 3550 5040 6112 7013
Size: 197 926 1401 107 99 1490 1072 901
Cuts at: 7013 7275 820
Size: 262 1553
Fragments arranged by size:
1553 1490 1401 1072 926 901 262 197
107 99
SnaBI TAC'GTA
Cuts at: 493 5799 493
Size: 5306 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8008
SphI G_CATG'C
Cuts at: 1132 2501 3966 4771 5366 1132
Size: 1369 1465 805 595 3774
Fragments arranged by size:
3774 1465 1369 805 595
SspI AAT'ATT
Cuts at: 5 52 5932 5
Size: 47 5880 2081
Fragments arranged by size:
5880 2081 47
StuI AGG'CCT
Cuts at: 1517 2291 2558 3796 4906 1517
Size: 774 267 1238 1110 4619
Fragments arranged by size:
4619 1238 1110 774 267
StyI C'CwwG_G
Cuts at: 513 1065 3321 3406 4047 4149 4444 5447 513
Size: 552 2256 85 641 102 295 1003 3074
Fragments arranged by size:
3074 2256 1003 641 552 295 102 85
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2049 2676 3131 3562 4745 6035 6052
Size: 1255 469 627 455 431 1183 1290 17
Cuts at: 6052 6205 6390 6835 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1290 1255 1183 627 469 455 445
431 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4360 5066 6254
Size: 274 80 33 51 1443 2383 706 1188
Cuts at: 6254 7966 96
Size: 1712 138
Fragments arranged by size:
2383 1712 1443 1188 706 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2258 2290 2672 2704 7968 8000 2258
Size: 32 382 32 5264 32 2266
Fragments arranged by size:
5264 2266 382 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8008
XbaI T'CTAG_A
Cuts at: 5344 5344
Size: 8008
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4295 4295
Size: 8008
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8008
XmaI C'CCGG_G
Cuts at: 1870 2709 5016 5333 1870
Size: 839 2307 317 4545
Fragments arranged by size:
4545 2307 839 317
XmnI GAAnn'nnTTC
Cuts at: 6137 6137
Size: 8008
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5816 276
Size: 53 83 186 936 4282 2468
Fragments arranged by size:
4282 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SalI SanDI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbeI BsiWI EcoRV FalI FseI
FspAI KasI NarI NruI PacI PspXI PsrI RsrII
SacII SexAI SfiI SfoI SrfI SwaI