Restriction map for
pFN21AF0188
(Circular) (Six-base) MAPSORT of: pFN21AF0188.seq Check: 3255 from: 1 to: 8536
pFN21AF0188 8536 bp
With 182 enzymes: SgfI *
February 27, 2009 14:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5182 5182
Size: 8536
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6346 278
Size: 53 83 186 936 4810 2468
Fragments arranged by size:
4810 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5066 5879 1937
Size: 3129 813 4594
Fragments arranged by size:
4594 3129 813
Acc65I G'GTAC_C
Cuts at: 2857 4266 5857 2857
Size: 1409 1591 5536
Fragments arranged by size:
5536 1591 1409
AclI AA'CG_TT
Cuts at: 1563 4167 6663 7036 1563
Size: 2604 2496 373 3063
Fragments arranged by size:
3063 2604 2496 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2375 2807 3041 3837 3918 4158 4216 6599
Size: 743 432 234 796 81 240 58 2383
Cuts at: 6599 7983 8251 1632
Size: 1384 268 1917
Fragments arranged by size:
2383 1917 1384 796 743 432 268 240
234 81 58
AflII C'TTAA_G
Cuts at: 820 1017 4819 820
Size: 197 3802 4537
Fragments arranged by size:
4537 3802 197
AflIII A'CryG_T
Cuts at: 1203 7435 8412 1203
Size: 6232 977 1327
Fragments arranged by size:
6232 1327 977
AgeI A'CCGG_T
Cuts at: 7367 7367
Size: 8536
AhdI GACnn_n'nnGTC
Cuts at: 7265 7265
Size: 8536
AleI CACnn'nnGTG
Cuts at: 1166 4821 5015 1166
Size: 3655 194 4687
Fragments arranged by size:
4687 3655 194
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2660 3311 5378 5562 1184
Size: 1476 651 2067 184 4158
Fragments arranged by size:
4158 2067 1476 651 184
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4448 4480 4448
Size: 32 8504
AlwNI CAG_nnn'CTG
Cuts at: 3279 3548 4375 4408 4578 5701 6279 7851 3279
Size: 269 827 33 170 1123 578 1572 3964
Fragments arranged by size:
3964 1572 1123 827 578 269 170 33
ApaI G_GGCC'C
Cuts at: 3028 3207 3028
Size: 179 8357
ApaLI G'TGCA_C
Cuts at: 6593 7749 6593
Size: 1156 7380
ApoI r'AATT_y
Cuts at: 1493 2376 3493 4760 5595 5845 6071 6223 8332
Size: 883 1117 1267 835 250 226 152 2109
Cuts at: 8332 1493
Size: 1697
Fragments arranged by size:
2109 1697 1267 1117 883 835 250 226
152
AseI AT'TA_AT
Cuts at: 160 1032 7090 160
Size: 872 6058 1606
Fragments arranged by size:
6058 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8536
AvaI C'yCGr_G
Cuts at: 1870 1943 2072 2264 2561 3443 4894 5861 1870
Size: 73 129 192 297 882 1451 967 4545
Fragments arranged by size:
4545 1451 967 882 297 192 129 73
AvrII C'CTAG_G
Cuts at: 3014 3014
Size: 8536
BamHI G'GATC_C
Cuts at: 2339 3562 5866 6233 2339
Size: 1223 2304 367 4642
Fragments arranged by size:
4642 2304 1223 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2032 2255 2516 2695 2857
Size: 325 217 627 245 223 261 179 162
Cuts at: 2857 3945 4057 4266 4898 5673 5857 7312 618
Size: 1088 112 209 632 775 184 1455 1842
Fragments arranged by size:
1842 1455 1088 775 632 627 325 261
245 223 217 209 184 179 162 112
BanII G_rGCy'C
Cuts at: 1391 2473 2489 2798 2936 3028 3207 3425 3434
Size: 1082 16 309 138 92 179 218 9
Cuts at: 3434 3443 3746 3937 4194 4914 5855 1391
Size: 9 303 191 257 720 941 4072
Fragments arranged by size:
4072 1082 941 720 309 303 257 218
191 179 138 92 16 9 9
BbeI G_GCGC'C
Cuts at: 3949 4902 3949
Size: 953 7583
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2956 3825 3855 3906 4248 5526 928
Size: 971 1057 869 30 51 342 1278 3938
Fragments arranged by size:
3938 1278 1057 971 869 342 51 30
BbvCI CC'TCA_GC
Cuts at: 5444 5444
Size: 8536
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5518 5552 6727 6761 1903
Size: 34 3581 34 1175 34 3678
Fragments arranged by size:
3678 3581 1175 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6428 7638 6428
Size: 1210 7326
BclI T'GATC_A
Cuts at: 1548 4308 5139 1548
Size: 2760 831 4945
Fragments arranged by size:
4945 2760 831
BfrBI ATG'CAT
Cuts at: 3145 3145
Size: 8536
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2175 7147 136
Size: 107 122 71 631 605 503 4972 1525
Fragments arranged by size:
4972 1525 631 605 503 122 107 71
BglII A'GATC_T
Cuts at: 5025 8531 5025
Size: 3506 5030
BlpI GC'TnA_GC
Cuts at: 3187 5953 3187
Size: 2766 5770
Bme1580I G_kGCm'C
Cuts at: 1790 2035 2260 2521 2543 2700 3028 3207 3484
Size: 245 225 261 22 157 328 179 277
Cuts at: 3484 5678 6597 7753 1790
Size: 2194 919 1156 2573
Fragments arranged by size:
2573 2194 1156 919 328 277 261 245
225 179 157 22
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8536
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4385 4527 6267
Size: 416 53 83 399 24 2968 142 1740
Cuts at: 6267 7225 442
Size: 958 1753
Fragments arranged by size:
2968 1753 1740 958 416 399 142 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 4727 1056
Size: 3671 4865
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2063 2947 3019 3057 3085 3697 3897 4504
Size: 824 884 72 38 28 612 200 607
Cuts at: 4504 4928 5183 7196 1239
Size: 424 255 2013 2579
Fragments arranged by size:
2579 2013 884 824 612 607 424 255
200 72 38 28
Bpu10I CC'TnA_GC
Cuts at: 2494 2873 3375 5444 2494
Size: 379 502 2069 5586
Fragments arranged by size:
5586 2069 502 379
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4808 5045 6661 7526 7824 4808
Size: 237 1616 865 298 5520
Fragments arranged by size:
5520 1616 865 298 237
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7199 882
Size: 617 5700 2219
Fragments arranged by size:
5700 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 6327 493
Size: 711 5123 2702
Fragments arranged by size:
5123 2702 711
BsaBI GATnn'nnATC
Cuts at: 4985 8530 4985
Size: 3545 4991
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2118 3946 4141 4899
Size: 53 83 186 936 585 1828 195 758
Cuts at: 4899 6343 6725 7360 275
Size: 1444 382 635 1451
Fragments arranged by size:
1828 1451 1444 936 758 635 585 382
195 186 83 53
BsaWI w'CCGG_w
Cuts at: 2328 5829 6969 7367 7641 7788 8199 2328
Size: 3501 1140 398 274 147 411 2665
Fragments arranged by size:
3501 2665 1140 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2995 3025 3071 3101 1178
Size: 30 165 30 1592 30 46 30 6613
Fragments arranged by size:
6613 1592 165 46 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2290 2770 2944 3016 3668 3748 4238 4553
Size: 1104 480 174 72 652 80 490 315
Cuts at: 4553 4565 1186
Size: 12 5157
Fragments arranged by size:
5157 1104 652 490 480 315 174 80
72 12
BseYI C'CCAG_C
Cuts at: 2209 5208 5748 7739 8235 2209
Size: 2999 540 1991 496 2510
Fragments arranged by size:
2999 2510 1991 540 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2118 3148 3265 5039 5568 5687 1163
Size: 573 382 1030 117 1774 529 119 4012
Fragments arranged by size:
4012 1774 1030 573 529 382 119 117
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5986 6747 7775 1540
Size: 275 186 3985 761 1028 2301
Fragments arranged by size:
3985 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2594 2768 2798 3110 4115 4914 6597 6682 7753
Size: 174 30 312 1005 799 1683 85 1071
Cuts at: 7753 2594
Size: 3377
Fragments arranged by size:
3377 1683 1071 1005 799 312 174 85
30
BsmI GAATG_Cn'
Cuts at: 3913 6047 6140 3913
Size: 2134 93 6309
Fragments arranged by size:
6309 2134 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3103 3745 3103
Size: 642 7894
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2035 2260 2473 2489 2521 2543 2594
Size: 399 245 225 213 16 32 22 51
Cuts at: 2594 2700 2768 2798 2936 3028 3110 3207 3425
Size: 106 68 30 138 92 82 97 218
Cuts at: 3425 3434 3443 3484 3746 3937 4115 4194 4914
Size: 9 9 41 262 191 178 79 720
Cuts at: 4914 5678 5855 6597 6682 7753 1391
Size: 764 177 742 85 1071 2174
Fragments arranged by size:
2174 1071 764 742 720 399 262 245
225 218 213 191 178 177 138 106
97 92 85 82 79 68 51 41
32 30 22 16 9 9
BspEI T'CCGG_A
Cuts at: 2328 5829 8199 2328
Size: 3501 2370 2665
Fragments arranged by size:
3501 2665 2370
BspHI T'CATG_A
Cuts at: 4923 6425 8155 4923
Size: 1502 1730 5304
Fragments arranged by size:
5304 1730 1502
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3170 4278 5182 5355 5746 5891 844
Size: 2326 1108 904 173 391 145 3489
Fragments arranged by size:
3489 2326 1108 904 391 173 145
BsrBI CCG'CTC
Cuts at: 1394 1823 2269 2601 4636 6423 1394
Size: 429 446 332 2035 1787 3507
Fragments arranged by size:
3507 2035 1787 446 429 332
BsrDI GCAATG_nn'
Cuts at: 65 1248 2986 7031 7205 65
Size: 1183 1738 4045 174 1396
Fragments arranged by size:
4045 1738 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3069 3629 4024 4143 5379 7180 7367
Size: 110 1259 560 395 119 1236 1801 187
Cuts at: 7367 1700
Size: 2869
Fragments arranged by size:
2869 1801 1259 1236 560 395 187 119
110
BsrGI T'GTAC_A
Cuts at: 96 4641 96
Size: 4545 3991
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8536
BssSI C'ACGA_G
Cuts at: 2717 6596 7608 2717
Size: 3879 1012 3645
Fragments arranged by size:
3879 3645 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2516 3455 4597 794
Size: 1722 939 1142 4733
Fragments arranged by size:
4733 1722 1142 939
BstBI TT'CG_AA
Cuts at: 7340 7340
Size: 8536
BstEII G'GTnAC_C
Cuts at: 1184 2128 1184
Size: 944 7592
BstXI CCAn_nnnn'nTGG
Cuts at: 2287 2535 2903 4126 2287
Size: 248 368 1223 6697
Fragments arranged by size:
6697 1223 368 248
BstYI r'GATC_y
Cuts at: 1972 2339 3562 4832 5025 5866 6233 6618 6635
Size: 367 1223 1270 193 841 367 385 17
Cuts at: 6635 8087 8173 8185 8531 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1270 1223 841 385 367 367
346 193 86 17 12
BstZ17I GTA'TAC
Cuts at: 5067 5067
Size: 8536
Bsu36I CC'TnA_GG
Cuts at: 2522 2834 4655 2522
Size: 312 1821 6403
Fragments arranged by size:
6403 1821 312
BtgI C'CryG_G
Cuts at: 513 1065 2280 3168 4359 5679 513
Size: 552 1215 888 1191 1320 3370
Fragments arranged by size:
3370 1320 1215 1191 888 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4972 487
Size: 683 56 3746 4051
Fragments arranged by size:
4051 3746 683 56
BtsI GCAGTG_nn'
Cuts at: 2055 3153 3222 4450 6052 6846 6866 2055
Size: 1098 69 1228 1602 794 20 3725
Fragments arranged by size:
3725 1602 1228 1098 794 69 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8536
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4717 4752 529
Size: 35 4153 35 4313
Fragments arranged by size:
4313 4153 35 35
DraI TTT'AAA
Cuts at: 5840 5840
Size: 8536
DraIII CAC_nnn'GTG
Cuts at: 3584 4057 3584
Size: 473 8063
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7543 809
Size: 507 6227 1802
Fragments arranged by size:
6227 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2689 3755 3767
Size: 54 1425 50 275 18 859 1066 12
Cuts at: 3767 3784 3792 4293 5049 5983 6872 8501 8
Size: 17 8 501 756 934 889 1629 43
Fragments arranged by size:
1629 1425 1066 934 889 859 756 501
275 54 50 43 18 17 12 8
EagI C'GGCC_G
Cuts at: 1537 1812 5983 1537
Size: 275 4171 4090
Fragments arranged by size:
4171 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2240 2786 3516 4497 5801 6466 1353
Size: 887 546 730 981 1304 665 3423
Fragments arranged by size:
3423 1304 981 887 730 665 546
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5786 7123 7497 7643 387
Size: 1064 138 4197 1337 374 146 1280
Fragments arranged by size:
4197 1337 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2796 4912 2796
Size: 2116 6420
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2063 2375 2807 2947 3019 3041 3057
Size: 393 431 312 432 140 72 22 16
Cuts at: 3057 3085 3697 3837 3897 3918 4158 4216 4504
Size: 28 612 140 60 21 240 58 288
Cuts at: 4504 4928 5183 6599 7196 7983 8251 1239
Size: 424 255 1416 597 787 268 1524
Fragments arranged by size:
1524 1416 787 612 597 432 431 424
393 312 288 268 255 240 140 140
72 60 58 28 22 21 16
EcoNI CCTnn'n_nnAGG
Cuts at: 3001 4250 5812 3001
Size: 1249 1562 5725
Fragments arranged by size:
5725 1562 1249
EcoO109I rG'GnC_Cy
Cuts at: 2081 2725 2868 3024 3025 3203 3204 3545 3601
Size: 644 143 156 1 178 1 341 56
Cuts at: 3601 3865 5344 5698 2081
Size: 264 1479 354 4919
Fragments arranged by size:
4919 1479 644 354 341 264 178 156
143 56 1 1
EcoRI G'AATT_C
Cuts at: 2376 3493 5845 6223 2376
Size: 1117 2352 378 4689
Fragments arranged by size:
4689 2352 1117 378
EcoRV GAT'ATC
Cuts at: 5224 5224
Size: 8536
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2815 3900 2815
Size: 1085 7451
FspI TGC'GCA
Cuts at: 4333 4719 5354 7042 4333
Size: 386 635 1688 5827
Fragments arranged by size:
5827 1688 635 386
HaeII r_GCGC'y
Cuts at: 1678 2460 3949 4902 5722 7683 1678
Size: 782 1489 953 820 1961 2531
Fragments arranged by size:
2531 1961 1489 953 820 782
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2995 3027 3071 3103 5208
Size: 32 135 32 1423 32 44 32 2105
Cuts at: 5208 5222 5240 5254 7177 7209 7251 7283 1373
Size: 14 18 14 1923 32 42 32 2626
Fragments arranged by size:
2626 2105 1923 1423 135 44 42 32
32 32 32 32 32 18 14 14
HincII GTy'rAC
Cuts at: 669 1657 1938 3363 5880 6126 669
Size: 988 281 1425 2517 246 3079
Fragments arranged by size:
3079 2517 1425 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2216 2900 748
Size: 1468 684 6384
Fragments arranged by size:
6384 1468 684
HpaI GTT'AAC
Cuts at: 6126 6126
Size: 8536
KasI G'GCGC_C
Cuts at: 3945 4898 3945
Size: 953 7583
KpnI G_GTAC'C
Cuts at: 2861 4270 5861 2861
Size: 1409 1591 5536
Fragments arranged by size:
5536 1591 1409
MfeI C'AATT_G
Cuts at: 6135 6135
Size: 8536
MluI A'CGCG_T
Cuts at: 8412 8412
Size: 8536
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2222 2580 4107 4188 4443 4491 4705 7650
Size: 917 358 1527 81 255 48 214 2945
Cuts at: 7650 7834 1305
Size: 184 2007
Fragments arranged by size:
2945 2007 1527 917 358 255 214 184
81 48
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2691 3794 4295 10
Size: 54 1425 343 859 1103 501 4251
Fragments arranged by size:
4251 1425 1103 859 501 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3585 3620 3956 4703 4821 5015 5729
Size: 648 2419 35 336 747 118 194 714
Cuts at: 5729 6494 6853 7012 518
Size: 765 359 159 2042
Fragments arranged by size:
2419 2042 765 747 714 648 359 336
194 159 118 35
MspA1I CmG'CkG
Cuts at: 1606 2587 3462 3790 4291 5041 5047 5299 5326
Size: 981 875 328 501 750 6 252 27
Cuts at: 5326 5952 6629 7777 8022 1606
Size: 626 677 1148 245 2120
Fragments arranged by size:
2120 1148 981 875 750 677 626 501
328 252 245 27 6
NaeI GCC'GGC
Cuts at: 3071 3631 4145 5381 3071
Size: 560 514 1236 6226
Fragments arranged by size:
6226 1236 560 514
NarI GG'CG_CC
Cuts at: 3946 4899 3946
Size: 953 7583
NcoI C'CATG_G
Cuts at: 513 1065 2280 4359 513
Size: 552 1215 2079 4690
Fragments arranged by size:
4690 2079 1215 552
NdeI CA'TA_TG
Cuts at: 387 5110 387
Size: 4723 3813
NgoMIV G'CCGG_C
Cuts at: 3069 3629 4143 5379 3069
Size: 560 514 1236 6226
Fragments arranged by size:
6226 1236 560 514
NheI G'CTAG_C
Cuts at: 1052 4723 1052
Size: 3671 4865
NotI GC'GGCC_GC
Cuts at: 5983 5983
Size: 8536
NsiI A_TGCA'T
Cuts at: 3147 3147
Size: 8536
NspI r_CATG'y
Cuts at: 1132 1229 2514 5365 5894 7439 1132
Size: 97 1285 2851 529 1545 2229
Fragments arranged by size:
2851 2229 1545 1285 529 97
PasI CC'CwG_GG
Cuts at: 1348 2028 1348
Size: 680 7856
PciI A'CATG_T
Cuts at: 7435 7435
Size: 8536
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2281 2513 2925 3035 3350 4375 4408 5326
Size: 1173 232 412 110 315 1025 33 918
Cuts at: 5326 1108
Size: 4318
Fragments arranged by size:
4318 1173 1025 918 412 315 232 110
33
PfoI T'CCnGG_A
Cuts at: 3101 3312 4614 3101
Size: 211 1302 7023
Fragments arranged by size:
7023 1302 211
PmeI GTTT'AAAC
Cuts at: 5840 5840
Size: 8536
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6605 6637 8118 8150 6605
Size: 32 1481 32 6991
Fragments arranged by size:
6991 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2725 2868 3601 3865 5344 2725
Size: 143 733 264 1479 5917
Fragments arranged by size:
5917 1479 733 264 143
PshAI GACnn'nnGTC
Cuts at: 1613 4607 1613
Size: 2994 5542
PsiI TTA'TAA
Cuts at: 6106 6106
Size: 8536
PspOMI G'GGCC_C
Cuts at: 3024 3203 3024
Size: 179 8357
PspXI vC'TCGA_Gb
Cuts at: 2561 2561
Size: 8536
PstI C_TGCA'G
Cuts at: 830 2168 3835 4275 4974 5046 5541 5888 830
Size: 1338 1667 440 699 72 495 347 3478
Fragments arranged by size:
3478 1667 1338 699 495 440 347 72
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8536
PvuII CAG'CTG
Cuts at: 3462 3790 4291 5047 5326 3462
Size: 328 501 756 279 6672
Fragments arranged by size:
6672 756 501 328 279
SacI G_AGCT'C
Cuts at: 2798 4914 2798
Size: 2116 6420
SalI G'TCGA_C
Cuts at: 1936 5878 1936
Size: 3942 4594
SanDI GG'GwC_CC
Cuts at: 2725 2725
Size: 8536
SapI GCTCTTCn'nnn_
Cuts at: 2786 3516 4497 5801 2786
Size: 730 981 1304 5521
Fragments arranged by size:
5521 1304 981 730
SbfI CC_TGCA'GG
Cuts at: 5888 5888
Size: 8536
ScaI AGT'ACT
Cuts at: 4880 6784 8496 4880
Size: 1904 1712 4920
Fragments arranged by size:
4920 1904 1712
SexAI A'CCwGG_T
Cuts at: 4053 4053
Size: 8536
SfcI C'TryA_G
Cuts at: 826 1045 2164 2749 3831 4271 4970 5042 5537
Size: 219 1119 585 1082 440 699 72 495
Cuts at: 5537 5884 7019 7700 7891 826
Size: 347 1135 681 191 1471
Fragments arranged by size:
1471 1135 1119 1082 699 681 585 495
440 347 219 191 72
SfoI GGC'GCC
Cuts at: 3947 4900 3947
Size: 953 7583
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8536
SgrAI Cr'CCGG_yG
Cuts at: 1700 4143 1700
Size: 2443 6093
SmaI CCC'GGG
Cuts at: 1872 3445 4896 5863 1872
Size: 1573 1451 967 4545
Fragments arranged by size:
4545 1573 1451 967
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2561 4787 4819 5060 6640 7541
Size: 197 926 618 2226 32 241 1580 901
Cuts at: 7541 7803 820
Size: 262 1553
Fragments arranged by size:
2226 1580 1553 926 901 618 262 241
197 32
SnaBI TAC'GTA
Cuts at: 493 6327 493
Size: 5834 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8536
SphI G_CATG'C
Cuts at: 1132 5894 1132
Size: 4762 3774
SspI AAT'ATT
Cuts at: 5 52 6460 5
Size: 47 6408 2081
Fragments arranged by size:
6408 2081 47
StuI AGG'CCT
Cuts at: 1517 3763 5543 1517
Size: 2246 1780 4510
Fragments arranged by size:
4510 2246 1780
StyI C'CwwG_G
Cuts at: 513 1065 2280 2946 3014 3758 4359 5975 513
Size: 552 1215 666 68 744 601 1616 3074
Fragments arranged by size:
3074 1616 1215 744 666 601 552 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2204 2689 3424 5247 6563 6580 6733
Size: 1255 624 485 735 1823 1316 17 153
Cuts at: 6733 6918 7363 325
Size: 185 445 1498
Fragments arranged by size:
1823 1498 1316 1255 735 624 485 445
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4641 4797 4878
Size: 274 80 33 51 1443 2664 156 81
Cuts at: 4878 4938 5367 6782 8494 96
Size: 60 429 1415 1712 138
Fragments arranged by size:
2664 1712 1443 1415 429 274 156 138
81 80 60 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4418 4450 8496 8528 4418
Size: 32 4046 32 4426
Fragments arranged by size:
4426 4046 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2960 1316
Size: 1644 6892
XbaI T'CTAG_A
Cuts at: 5872 5872
Size: 8536
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2287 5125 2287
Size: 2838 5698
XhoI C'TCGA_G
Cuts at: 1943 2561 1943
Size: 618 7918
XmaI C'CCGG_G
Cuts at: 1870 3443 4894 5861 1870
Size: 1573 1451 967 4545
Fragments arranged by size:
4545 1573 1451 967
XmnI GAAnn'nnTTC
Cuts at: 5247 6665 5247
Size: 1418 7118
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6344 276
Size: 53 83 186 936 4810 2468
Fragments arranged by size:
4810 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BplI BsiWI FseI FspAI NruI
PacI PmlI PsrI RsrII SacII SfiI SrfI SwaI