Restriction map for
pFN21AF0175
(Circular) (Six-base) MAPSORT of: pFN21AF0175.seq Check: 2609 from: 1 to: 11062
pFN21AF0175 11062 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2356 2356
Size: 11062
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4779 8872 278
Size: 53 83 186 936 3243 4093 2468
Fragments arranged by size:
4093 3243 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2961 7113 7482 8405 1937
Size: 1024 4152 369 923 4594
Fragments arranged by size:
4594 4152 1024 923 369
Acc65I G'GTAC_C
Cuts at: 8383 8383
Size: 11062
AclI AA'CG_TT
Cuts at: 1563 9189 9562 1563
Size: 7626 373 3063
Fragments arranged by size:
7626 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3195 3269 4166 4352 6647 7370 8341 9125
Size: 1563 74 897 186 2295 723 971 784
Cuts at: 9125 10509 10777 1632
Size: 1384 268 1917
Fragments arranged by size:
2295 1917 1563 1384 971 897 784 723
268 186 74
AfeI AGC'GCT
Cuts at: 6042 6042
Size: 11062
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10865
AflIII A'CryG_T
Cuts at: 1203 5529 9961 10938 1203
Size: 4326 4432 977 1327
Fragments arranged by size:
4432 4326 1327 977
AgeI A'CCGG_T
Cuts at: 9893 9893
Size: 11062
AhdI GACnn_n'nnGTC
Cuts at: 6481 9791 6481
Size: 3310 7752
AleI CACnn'nnGTG
Cuts at: 1166 4979 1166
Size: 3813 7249
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 11062
AlwNI CAG_nnn'CTG
Cuts at: 3077 4228 4276 5711 7399 8805 10377 3077
Size: 1151 48 1435 1688 1406 1572 3762
Fragments arranged by size:
3762 1688 1572 1435 1406 1151 48
ApaI G_GGCC'C
Cuts at: 3618 5404 7355 3618
Size: 1786 1951 7325
Fragments arranged by size:
7325 1951 1786
ApaLI G'TGCA_C
Cuts at: 9119 10275 9119
Size: 1156 9906
ApoI r'AATT_y
Cuts at: 1493 6560 6752 6771 8371 8597 8749 10858 1493
Size: 5067 192 19 1600 226 152 2109 1697
Fragments arranged by size:
5067 2109 1697 1600 226 192 152 19
AseI AT'TA_AT
Cuts at: 160 1032 9616 160
Size: 872 8584 1606
Fragments arranged by size:
8584 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11062
AvaI C'yCGr_G
Cuts at: 1870 1943 2638 2693 2993 5300 5541 8387 1870
Size: 73 695 55 300 2307 241 2846 4545
Fragments arranged by size:
4545 2846 2307 695 300 241 73 55
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 7250 7283 7250
Size: 33 11029
BamHI G'GATC_C
Cuts at: 2859 5949 7332 8392 8759 2859
Size: 3090 1383 1060 367 5162
Fragments arranged by size:
5162 3090 1383 1060 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2787 3017 3990 4483 4517
Size: 325 217 627 1000 230 973 493 34
Cuts at: 4517 4585 4592 5340 7258 8178 8383 9838 618
Size: 68 7 748 1918 920 205 1455 1842
Fragments arranged by size:
1918 1842 1455 1000 973 920 748 627
493 325 230 217 205 68 34 7
BanII G_rGCy'C
Cuts at: 1391 2195 2430 2647 2943 3036 3238 3618 3684
Size: 804 235 217 296 93 202 380 66
Cuts at: 3684 4206 4353 4416 4638 5404 5886 6246 6453
Size: 522 147 63 222 766 482 360 207
Cuts at: 6453 6996 7150 7355 7892 8148 8381 1391
Size: 543 154 205 537 256 233 4072
Fragments arranged by size:
4072 804 766 543 537 522 482 380
360 296 256 235 233 222 217 207
205 202 154 147 93 66 63
BbeI G_GCGC'C
Cuts at: 2791 4596 5344 8182 2791
Size: 1805 748 2838 5671
Fragments arranged by size:
5671 2838 1805 748
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2580 3183 3247 3413 4871 5372 5624
Size: 971 681 603 64 166 1458 501 252
Cuts at: 5624 6426 6795 928
Size: 802 369 5195
Fragments arranged by size:
5195 1458 971 802 681 603 501 369
252 166 64
BbvCI CC'TCA_GC
Cuts at: 4671 4671
Size: 11062
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 9253 9287 1903
Size: 34 50 34 7232 34 3678
Fragments arranged by size:
7232 3678 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 8954 10164 8954
Size: 1210 9852
BclI T'GATC_A
Cuts at: 1548 1548
Size: 11062
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4148 5028 6291
Size: 107 122 71 631 605 2476 880 1263
Cuts at: 6291 9673 136
Size: 3382 1525
Fragments arranged by size:
3382 2476 1525 1263 880 631 605 122
107 71
BglII A'GATC_T
Cuts at: 3312 3396 5685 6933 7848 11057 3312
Size: 84 2289 1248 915 3209 3317
Fragments arranged by size:
3317 3209 2289 1248 915 84
BlpI GC'TnA_GC
Cuts at: 4188 5712 7761 8479 4188
Size: 1524 2049 718 6771
Fragments arranged by size:
6771 2049 1524 718
Bme1580I G_kGCm'C
Cuts at: 1790 2400 3020 3618 4402 4488 4745 5024 5404
Size: 610 620 598 784 86 257 279 380
Cuts at: 5404 7355 9123 10279 1790
Size: 1951 1768 1156 2573
Fragments arranged by size:
2573 1951 1768 1156 784 620 610 598
380 279 257 86
BmgBI CAC'GTC
Cuts at: 1318 2675 3013 4171 6424 7687 1318
Size: 1357 338 1158 2253 1263 4693
Fragments arranged by size:
4693 2253 1357 1263 1158 338
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2037 3505 4060
Size: 416 53 83 399 24 620 1468 555
Cuts at: 4060 4747 5036 5146 7349 8793 9751 442
Size: 687 289 110 2203 1444 958 1753
Fragments arranged by size:
2203 1753 1468 1444 958 687 620 555
416 399 289 110 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 11062
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2297 4267 5290 2297
Size: 1970 1023 8069
Fragments arranged by size:
8069 1970 1023
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2409 2444 2619 3176 3585 3758 4145 4385
Size: 1170 35 175 557 409 173 387 240
Cuts at: 4385 4860 5381 6029 6353 6626 6668 7010 7058
Size: 475 521 648 324 273 42 342 48
Cuts at: 7058 7139 7747 7819 9722 1239
Size: 81 608 72 1903 2579
Fragments arranged by size:
2579 1903 1170 648 608 557 521 475
409 387 342 324 273 240 175 173
81 72 48 42 35
Bpu10I CC'TnA_GC
Cuts at: 4301 4671 4898 7604 8160 4301
Size: 370 227 2706 556 7203
Fragments arranged by size:
7203 2706 556 370 227
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2153 5414 5476 8186 9187 10052 10350 2153
Size: 3261 62 2710 1001 865 298 2865
Fragments arranged by size:
3261 2865 2710 1001 865 298 62
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3734 9725 882
Size: 617 2235 5991 2219
Fragments arranged by size:
5991 2235 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 7414 8853 493
Size: 711 6210 1439 2702
Fragments arranged by size:
6210 2702 1439 711
BsaBI GATnn'nnATC
Cuts at: 3485 5684 11056 3485
Size: 2199 5372 3491
Fragments arranged by size:
5372 3491 2199
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2788 3885 4593 4776
Size: 53 83 186 936 1255 1097 708 183
Cuts at: 4776 4887 5341 8179 8869 9251 9886 275
Size: 111 454 2838 690 382 635 1451
Fragments arranged by size:
2838 1451 1255 1097 936 708 690 635
454 382 186 183 111 83 53
BsaWI w'CCGG_w
Cuts at: 3003 4858 6180 7269 8250 9495 9893 10167 10314
Size: 1855 1322 1089 981 1245 398 274 147
Cuts at: 10314 10725 3003
Size: 411 3340
Fragments arranged by size:
3340 1855 1322 1245 1089 981 411 398
274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 6101 6131 6496 6526 7064
Size: 30 165 30 4698 30 365 30 538
Cuts at: 7064 7094 7853 7883 1178
Size: 30 759 30 4357
Fragments arranged by size:
4698 4357 759 538 365 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2409 2580 2981 3246 3582 4424 6106 6254
Size: 1223 171 401 265 336 842 1682 148
Cuts at: 6254 6504 6737 7067 7214 7376 8129 1186
Size: 250 233 330 147 162 753 4119
Fragments arranged by size:
4119 1682 1223 842 753 401 336 330
265 250 233 171 162 148 147
BseYI C'CCAG_C
Cuts at: 3092 3130 5091 5181 5262 5723 6122 6793 7218
Size: 38 1961 90 81 461 399 671 425
Cuts at: 7218 7828 10265 10761 3092
Size: 610 2437 496 3393
Fragments arranged by size:
3393 2437 1961 671 610 496 461 425
399 90 81 38
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3409 3996 4628 5588 5784 5954 6176
Size: 573 1673 587 632 960 196 170 222
Cuts at: 6176 6190 6967 7009 1163
Size: 14 777 42 5216
Fragments arranged by size:
5216 1673 960 777 632 587 573 222
196 170 42 14
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4824 7110 8512 9273 10301 1540
Size: 275 186 2823 2286 1402 761 1028 2301
Fragments arranged by size:
2823 2301 2286 1402 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3684 3759 4160 4206 4353 5886 6246 6429 7413
Size: 75 401 46 147 1533 360 183 984
Cuts at: 7413 7949 9123 9208 10279 3684
Size: 536 1174 85 1071 4467
Fragments arranged by size:
4467 1533 1174 1071 984 536 401 360
183 147 85 75 46
BsmI GAATG_Cn'
Cuts at: 8573 8666 8573
Size: 93 10969
BsmBI CGTCTCn'nnnn_
Cuts at: 2219 5452 5599 7097 2219
Size: 3233 147 1498 6184
Fragments arranged by size:
6184 3233 1498 147
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2195 2400 2430 2647 2943 3020 3036
Size: 399 405 205 30 217 296 77 16
Cuts at: 3036 3238 3618 3684 3759 4160 4206 4353 4402
Size: 202 380 66 75 401 46 147 49
Cuts at: 4402 4416 4488 4638 4745 5024 5404 5886 6246
Size: 14 72 150 107 279 380 482 360
Cuts at: 6246 6429 6453 6996 7150 7355 7413 7892 7949
Size: 183 24 543 154 205 58 479 57
Cuts at: 7949 8148 8381 9123 9208 10279 1391
Size: 199 233 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 543 482 479 405 401
399 380 380 360 296 279 233 217
205 205 202 199 183 154 150 147
107 85 77 75 72 66 58 57
49 46 30 24 16 14
BspEI T'CCGG_A
Cuts at: 3003 4858 6180 10725 3003
Size: 1855 1322 4545 3340
Fragments arranged by size:
4545 3340 1855 1322
BspHI T'CATG_A
Cuts at: 6529 8951 10681 6529
Size: 2422 1730 6910
Fragments arranged by size:
6910 2422 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2356 3210 3947 4120 5985 7031 7761 8417
Size: 1512 854 737 173 1865 1046 730 656
Cuts at: 8417 844
Size: 3489
Fragments arranged by size:
3489 1865 1512 1046 854 737 730 656
173
BsrBI CCG'CTC
Cuts at: 1394 1823 5122 6327 8119 8949 1394
Size: 429 3299 1205 1792 830 3507
Fragments arranged by size:
3507 3299 1792 1205 830 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 7776 9557 9731 65
Size: 1183 6528 1781 174 1396
Fragments arranged by size:
6528 1781 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2058 2545 3720 4107 4823 4889 5698
Size: 110 248 487 1175 387 716 66 809
Cuts at: 5698 5902 6297 9706 9893 1700
Size: 204 395 3409 187 2869
Fragments arranged by size:
3409 2869 1175 809 716 487 395 387
248 204 187 110 66
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 11062
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 11062
BssSI C'ACGA_G
Cuts at: 2521 6128 7833 9122 10134 2521
Size: 3607 1705 1289 1012 3449
Fragments arranged by size:
3607 3449 1705 1289 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 11062
BstBI TT'CG_AA
Cuts at: 9866 9866
Size: 11062
BstEII G'GTnAC_C
Cuts at: 1184 2827 4892 1184
Size: 1643 2065 7354
Fragments arranged by size:
7354 2065 1643
BstXI CCAn_nnnn'nTGG
Cuts at: 3751 4094 4264 5031 5731 5921 6130 8344 3751
Size: 343 170 767 700 190 209 2214 6469
Fragments arranged by size:
6469 2214 767 700 343 209 190 170
BstYI r'GATC_y
Cuts at: 1972 2859 3312 3396 4953 5203 5685 5949 6733
Size: 887 453 84 1557 250 482 264 784
Cuts at: 6733 6933 7332 7848 8392 8759 9144 9161 10613
Size: 200 399 516 544 367 385 17 1452
Cuts at: 10613 10699 10711 11057 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1557 1452 887 784 544 516 482
453 399 385 367 346 264 250 200
86 84 17 12
Bsu36I CC'TnA_GG
Cuts at: 3818 3818
Size: 11062
BtgI C'CryG_G
Cuts at: 513 1065 2514 2553 4161 5816 7535 513
Size: 552 1449 39 1608 1655 1719 4040
Fragments arranged by size:
4040 1719 1655 1608 1449 552 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4192 5570 487
Size: 683 56 2966 1378 5979
Fragments arranged by size:
5979 2966 1378 683 56
BtsI GCAGTG_nn'
Cuts at: 3440 4160 8578 9372 9392 3440
Size: 720 4418 794 20 5110
Fragments arranged by size:
5110 4418 794 720 20
ClaI AT'CG_AT
Cuts at: 1554 6276 6546 1554
Size: 4722 270 6070
Fragments arranged by size:
6070 4722 270
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4510 4522 4545 4557 529
Size: 35 3946 12 23 12 7034
Fragments arranged by size:
7034 3946 35 23 12 12
DraI TTT'AAA
Cuts at: 8366 8366
Size: 11062
DraIII CAC_nnn'GTG
Cuts at: 6383 6383
Size: 11062
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6157 10069 809
Size: 507 4841 3912 1802
Fragments arranged by size:
4841 3912 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2543 3411 3718
Size: 54 1425 50 275 18 713 868 307
Cuts at: 3718 4339 4821 5700 5904 6105 6292 6831 8128
Size: 621 482 879 204 201 187 539 1297
Cuts at: 8128 8301 8509 9398 11027 8
Size: 173 208 889 1629 43
Fragments arranged by size:
1629 1425 1297 889 879 868 713 621
539 482 307 275 208 204 201 187
173 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4821 8509 1537
Size: 275 3009 3688 4090
Fragments arranged by size:
4090 3688 3009 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2099 2888 4508 4905 6086 6218 6531 6770
Size: 746 789 1620 397 1181 132 313 239
Cuts at: 6770 7238 7308 8037 8273 8285 8992 1353
Size: 468 70 729 236 12 707 3423
Fragments arranged by size:
3423 1620 1181 789 746 729 707 468
397 313 239 236 132 70 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2290 2624 2670 4388 4802 5258
Size: 1064 138 701 334 46 1718 414 456
Cuts at: 5258 5264 5315 6377 7199 9649 10023 10169 387
Size: 6 51 1062 822 2450 374 146 1280
Fragments arranged by size:
2450 1718 1280 1064 1062 822 701 456
414 374 334 146 138 51 46 6
EcoICRI GAG'CTC
Cuts at: 3682 4204 4351 5884 6244 3682
Size: 522 147 1533 360 8500
Fragments arranged by size:
8500 1533 522 360 147
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2409 2444 2619 3176 3195 3269 3585
Size: 393 777 35 175 557 19 74 316
Cuts at: 3585 3758 4145 4166 4352 4385 4860 5381 6029
Size: 173 387 21 186 33 475 521 648
Cuts at: 6029 6353 6626 6647 6668 7010 7058 7139 7370
Size: 324 273 21 21 342 48 81 231
Cuts at: 7370 7747 7819 8341 9125 9722 10509 10777 1239
Size: 377 72 522 784 597 787 268 1524
Fragments arranged by size:
1524 787 784 777 648 597 557 522
521 475 393 387 377 342 324 316
273 268 231 186 175 173 81 74
72 48 35 33 21 21 21 19
EcoNI CCTnn'n_nnAGG
Cuts at: 2186 3049 4127 4406 6608 7082 7798 2186
Size: 863 1078 279 2202 474 716 5450
Fragments arranged by size:
5450 2202 1078 863 716 474 279
EcoO109I rG'GnC_Cy
Cuts at: 2164 2263 2278 2349 3134 3615 3778 5140 5372
Size: 99 15 71 785 481 163 1362 232
Cuts at: 5372 5400 5401 5545 6076 6670 6760 6842 6901
Size: 28 1 144 531 594 90 82 59
Cuts at: 6901 7045 7396 7498 8170 2164
Size: 144 351 102 672 5056
Fragments arranged by size:
5056 1362 785 672 594 531 481 351
232 163 144 144 102 99 90 82
71 59 28 15 1
EcoRI G'AATT_C
Cuts at: 8371 8749 8371
Size: 378 10684
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2103 3358 4825 6781 7810 7888 7966 2103
Size: 1255 1467 1956 1029 78 78 5199
Fragments arranged by size:
5199 1956 1467 1255 1029 78 78
FseI GG_CCGG'CC
Cuts at: 2549 2549
Size: 11062
FspI TGC'GCA
Cuts at: 4289 5195 9568 4289
Size: 906 4373 5783
Fragments arranged by size:
5783 4373 906
HaeII r_GCGC'y
Cuts at: 1678 2791 3152 4596 5182 5344 6044 8182 10209
Size: 1113 361 1444 586 162 700 2138 2027
Cuts at: 10209 1678
Size: 2531
Fragments arranged by size:
2531 2138 2027 1444 1113 700 586 361
162
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2177 2209 2477 2509 2971
Size: 32 135 32 605 32 268 32 462
Cuts at: 2971 3003 3071 3103 4160 4192 5039 5071 5426
Size: 32 68 32 1057 32 847 32 355
Cuts at: 5426 5458 5552 5584 6341 6356 6373 6388 6467
Size: 32 94 32 757 15 17 15 79
Cuts at: 6467 6499 6659 6691 6718 6750 7012 7044 7064
Size: 32 160 32 27 32 262 32 20
Cuts at: 7064 7096 7575 7607 7853 7885 9703 9735 9777
Size: 32 479 32 246 32 1818 32 42
Cuts at: 9777 9809 1373
Size: 32 2626
Fragments arranged by size:
2626 1818 1057 847 757 605 479 462
355 268 262 246 160 135 94 79
68 42 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 27 20 17
15 15
HincII GTy'rAC
Cuts at: 669 1657 1938 5795 7114 7483 8406 8652 669
Size: 988 281 3857 1319 369 923 246 3079
Fragments arranged by size:
3857 3079 1319 988 923 369 281 246
HindIII A'AGCT_T
Cuts at: 748 8162 748
Size: 7414 3648
HpaI GTT'AAC
Cuts at: 8652 8652
Size: 11062
KasI G'GCGC_C
Cuts at: 2787 4592 5340 8178 2787
Size: 1805 748 2838 5671
Fragments arranged by size:
5671 2838 1805 748
KpnI G_GTAC'C
Cuts at: 8387 8387
Size: 11062
MfeI C'AATT_G
Cuts at: 8661 8661
Size: 11062
MluI A'CGCG_T
Cuts at: 10938 10938
Size: 11062
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7628 10176 10360 1305
Size: 6323 2548 184 2007
Fragments arranged by size:
6323 2548 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 6107 8130 8303 10
Size: 54 1425 343 4275 2023 173 2769
Fragments arranged by size:
4275 2769 2023 1425 343 173 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4445 4979 5732 6014 6221 6803 7637
Size: 648 3279 534 753 282 207 582 834
Cuts at: 7637 9020 9379 9538 518
Size: 1383 359 159 2042
Fragments arranged by size:
3279 2042 1383 834 753 648 582 534
359 282 207 159
MspA1I CmG'CkG
Cuts at: 1606 2383 2794 2834 3949 4090 4192 4285 4480
Size: 777 411 40 1115 141 102 93 195
Cuts at: 4480 5337 5381 6103 6646 6829 7760 7960 8478
Size: 857 44 722 543 183 931 200 518
Cuts at: 8478 9155 10303 10548 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 1115 931 857 777 722 677
543 518 411 245 200 195 183 141
102 93 44 40
NaeI GCC'GGC
Cuts at: 2547 3722 4825 5700 5904 6299 2547
Size: 1175 1103 875 204 395 7310
Fragments arranged by size:
7310 1175 1103 875 395 204
NarI GG'CG_CC
Cuts at: 2788 4593 5341 8179 2788
Size: 1805 748 2838 5671
Fragments arranged by size:
5671 2838 1805 748
NcoI C'CATG_G
Cuts at: 513 1065 2514 513
Size: 552 1449 9061
Fragments arranged by size:
9061 1449 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 11062
NgoMIV G'CCGG_C
Cuts at: 2545 3720 4823 5698 5902 6297 2545
Size: 1175 1103 875 204 395 7310
Fragments arranged by size:
7310 1175 1103 875 395 204
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 11062
NotI GC'GGCC_GC
Cuts at: 8509 8509
Size: 11062
NspI r_CATG'y
Cuts at: 1132 1229 5533 7382 7918 8420 9965 1132
Size: 97 4304 1849 536 502 1545 2229
Fragments arranged by size:
4304 2229 1849 1545 536 502 97
PasI CC'CwG_GG
Cuts at: 1348 4011 5404 6234 6838 7501 7702 1348
Size: 2663 1393 830 604 663 201 4708
Fragments arranged by size:
4708 2663 1393 830 663 604 201
PciI A'CATG_T
Cuts at: 5529 9961 5529
Size: 4432 6630
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4228 4882 6383 6829 1108
Size: 3120 654 1501 446 5341
Fragments arranged by size:
5341 3120 1501 654 446
PfoI T'CCnGG_A
Cuts at: 2619 4939 5952 7404 7593 2619
Size: 2320 1013 1452 189 6088
Fragments arranged by size:
6088 2320 1452 1013 189
PmeI GTTT'AAAC
Cuts at: 8366 8366
Size: 11062
PmlI CAC'GTG
Cuts at: 7414 7414
Size: 11062
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4640 4672 6524 6556 9131 9163 10644 10676 4640
Size: 32 1852 32 2575 32 1481 32 5026
Fragments arranged by size:
5026 2575 1852 1481 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2164 2263 2278 3778 5140 5545 6842 6901 7045
Size: 99 15 1500 1362 405 1297 59 144
Cuts at: 7045 8170 2164
Size: 1125 5056
Fragments arranged by size:
5056 1500 1362 1297 1125 405 144 99
59 15
PshAI GACnn'nnGTC
Cuts at: 1613 4890 7109 1613
Size: 3277 2219 5566
Fragments arranged by size:
5566 3277 2219
PsiI TTA'TAA
Cuts at: 8632 8632
Size: 11062
PspOMI G'GGCC_C
Cuts at: 3614 5400 7351 3614
Size: 1786 1951 7325
Fragments arranged by size:
7325 1951 1786
PspXI vC'TCGA_Gb
Cuts at: 5300 5300
Size: 11062
PstI C_TGCA'G
Cuts at: 830 2367 2839 3873 4220 4360 4455 5380 6894
Size: 1537 472 1034 347 140 95 925 1514
Cuts at: 6894 7446 7473 7899 8414 830
Size: 552 27 426 515 3478
Fragments arranged by size:
3478 1537 1514 1034 925 552 515 472
426 347 140 95 27
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11062
PvuII CAG'CTG
Cuts at: 2794 4090 4192 4285 5381 6103 6646 6829 7760
Size: 1296 102 93 1096 722 543 183 931
Cuts at: 7760 7960 2794
Size: 200 5896
Fragments arranged by size:
5896 1296 1096 931 722 543 200 183
102 93
SacI G_AGCT'C
Cuts at: 3684 4206 4353 5886 6246 3684
Size: 522 147 1533 360 8500
Fragments arranged by size:
8500 1533 522 360 147
SalI G'TCGA_C
Cuts at: 1936 7112 7481 8404 1936
Size: 5176 369 923 4594
Fragments arranged by size:
5176 4594 923 369
SanDI GG'GwC_CC
Cuts at: 5140 6842 5140
Size: 1702 9360
SbfI CC_TGCA'GG
Cuts at: 2367 8414 2367
Size: 6047 5015
ScaI AGT'ACT
Cuts at: 3833 9310 11022 3833
Size: 5477 1712 3873
Fragments arranged by size:
5477 3873 1712
SfcI C'TryA_G
Cuts at: 826 1045 2363 2835 3869 4216 4356 4451 4550
Size: 219 1318 472 1034 347 140 95 99
Cuts at: 4550 5376 6890 7432 7442 7469 7895 8410 9545
Size: 826 1514 542 10 27 426 515 1135
Cuts at: 9545 10226 10417 826
Size: 681 191 1471
Fragments arranged by size:
1514 1471 1318 1135 1034 826 681 542
515 472 426 347 219 191 140 99
95 27 10
SfoI GGC'GCC
Cuts at: 2789 4594 5342 8180 2789
Size: 1805 748 2838 5671
Fragments arranged by size:
5671 2838 1805 748
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11062
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11062
SmaI CCC'GGG
Cuts at: 1872 2640 2695 5543 8389 1872
Size: 768 55 2848 2846 4545
Fragments arranged by size:
4545 2848 2846 768 55
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2168 5300 5393 5455 8165 9166
Size: 197 926 225 3132 93 62 2710 1001
Cuts at: 9166 10067 10329 820
Size: 901 262 1553
Fragments arranged by size:
3132 2710 1553 1001 926 901 262 225
197 93 62
SnaBI TAC'GTA
Cuts at: 493 8853 493
Size: 8360 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 11062
SphI G_CATG'C
Cuts at: 1132 7918 8420 1132
Size: 6786 502 3774
Fragments arranged by size:
6786 3774 502
SspI AAT'ATT
Cuts at: 5 52 7304 8986 5
Size: 47 7252 1682 2081
Fragments arranged by size:
7252 2081 1682 47
StuI AGG'CCT
Cuts at: 1517 6662 1517
Size: 5145 5917
StyI C'CwwG_G
Cuts at: 513 1065 2259 2514 2657 4800 5004 6897 7083
Size: 552 1194 255 143 2143 204 1893 186
Cuts at: 7083 7950 8085 8174 8501 513
Size: 867 135 89 327 3074
Fragments arranged by size:
3074 2143 1893 1194 867 552 327 255
204 186 143 135 89
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4220 4274 4307 5173 7201 9089 9106
Size: 1255 2640 54 33 866 2028 1888 17
Cuts at: 9106 9259 9444 9889 325
Size: 153 185 445 1498
Fragments arranged by size:
2640 2028 1888 1498 1255 866 445 185
153 54 33 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3831 3966 5157
Size: 274 80 33 51 1443 1854 135 1191
Cuts at: 5157 9308 11020 96
Size: 4151 1712 138
Fragments arranged by size:
4151 1854 1712 1443 1191 274 138 135
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6496 6528 7264 7296 11022 11054 6496
Size: 32 736 32 3726 32 6504
Fragments arranged by size:
6504 3726 736 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2072 2491 4436 1316
Size: 756 419 1945 7942
Fragments arranged by size:
7942 1945 756 419
XbaI T'CTAG_A
Cuts at: 2726 8398 2726
Size: 5672 5390
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2594 4168 5731 5756 5823 5918 6013 6064 6328
Size: 1574 1563 25 67 95 95 51 264
Cuts at: 6328 2594
Size: 7328
Fragments arranged by size:
7328 1574 1563 264 95 95 67 51
25
XhoI C'TCGA_G
Cuts at: 1943 5300 1943
Size: 3357 7705
XmaI C'CCGG_G
Cuts at: 1870 2638 2693 5541 8387 1870
Size: 768 55 2848 2846 4545
Fragments arranged by size:
4545 2848 2846 768 55
XmnI GAAnn'nnTTC
Cuts at: 2243 3254 3926 4655 8354 9191 2243
Size: 1011 672 729 3699 837 4114
Fragments arranged by size:
4114 3699 1011 837 729 672
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4777 8870 276
Size: 53 83 186 936 3243 4093 2468
Fragments arranged by size:
4093 3243 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI
SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BfrBI BsiWI BstZ17I EcoRV FspAI
NruI NsiI PacI PsrI RsrII SacII SapI SexAI
SfiI SrfI SwaI