Restriction map for
pFN21AF0165
(Circular) (Six-base) MAPSORT of: pFN21AF0165.seq Check: 3365 from: 1 to: 5515
pFN21AF0165 5515 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3325 278
Size: 53 83 186 936 1789 2468
Fragments arranged by size:
2468 1789 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2661 2858 1937
Size: 724 197 4594
Fragments arranged by size:
4594 724 197
Acc65I G'GTAC_C
Cuts at: 2463 2836 2463
Size: 373 5142
AclI AA'CG_TT
Cuts at: 1563 3642 4015 1563
Size: 2079 373 3063
Fragments arranged by size:
3063 2079 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2176 2634 3578 4962 5230 1632
Size: 544 458 944 1384 268 1917
Fragments arranged by size:
1917 1384 944 544 458 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5318
AflIII A'CryG_T
Cuts at: 1203 4414 5391 1203
Size: 3211 977 1327
Fragments arranged by size:
3211 1327 977
AgeI A'CCGG_T
Cuts at: 4346 4346
Size: 5515
AhdI GACnn_n'nnGTC
Cuts at: 4244 4244
Size: 5515
AleI CACnn'nnGTG
Cuts at: 1166 2357 1166
Size: 1191 4324
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2567 1184
Size: 1383 4132
AlwNI CAG_nnn'CTG
Cuts at: 2657 3258 4830 2657
Size: 601 1572 3342
Fragments arranged by size:
3342 1572 601
ApaI G_GGCC'C
Cuts at: 2035 2035
Size: 5515
ApaLI G'TGCA_C
Cuts at: 3572 4728 3572
Size: 1156 4359
ApoI r'AATT_y
Cuts at: 1493 2824 3050 3202 5311 1493
Size: 1331 226 152 2109 1697
Fragments arranged by size:
2109 1697 1331 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4069 160
Size: 872 3037 1606
Fragments arranged by size:
3037 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5515
AvaI C'yCGr_G
Cuts at: 1870 1943 2105 2840 1870
Size: 73 162 735 4545
Fragments arranged by size:
4545 735 162 73
BamHI G'GATC_C
Cuts at: 2845 3212 2845
Size: 367 5148
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2384 2435 2463 2836 4291
Size: 325 217 627 597 51 28 373 1455
Cuts at: 4291 618
Size: 1842
Fragments arranged by size:
1842 1455 627 597 373 325 217 51
28
BanII G_rGCy'C
Cuts at: 1391 2035 2064 2282 2475 2747 2834 1391
Size: 644 29 218 193 272 87 4072
Fragments arranged by size:
4072 644 272 218 193 87 29
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4544
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2678 2712 3706 3740 1903
Size: 34 741 34 994 34 3678
Fragments arranged by size:
3678 994 741 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3407 4617 3407
Size: 1210 4305
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5515
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2171 4126 136
Size: 107 122 71 631 605 499 1955 1525
Fragments arranged by size:
1955 1525 631 605 499 122 107 71
BglII A'GATC_T
Cuts at: 2260 2621 5510 2260
Size: 361 2889 2265
Fragments arranged by size:
2889 2265 361
BlpI GC'TnA_GC
Cuts at: 2932 2932
Size: 5515
Bme1580I G_kGCm'C
Cuts at: 1790 2035 2318 2546 2612 3576 4732 1790
Size: 245 283 228 66 964 1156 2573
Fragments arranged by size:
2573 1156 964 283 245 228 66
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5515
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2321 3246 4204
Size: 416 53 83 399 24 904 925 958
Cuts at: 4204 442
Size: 1753
Fragments arranged by size:
1753 958 925 904 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5515
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2249 4175 1239
Size: 1010 1926 2579
Fragments arranged by size:
2579 1926 1010
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3640 4505 4803 3640
Size: 865 298 4352
Fragments arranged by size:
4352 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2678 2755 4178 882
Size: 617 1179 77 1423 2219
Fragments arranged by size:
2219 1423 1179 617 77
BsaAI yAC'GTr
Cuts at: 493 1204 3306 493
Size: 711 2102 2702
Fragments arranged by size:
2702 2102 711
BsaBI GATnn'nnATC
Cuts at: 5509 5509
Size: 5515
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3322 3704 4339 275
Size: 53 83 186 936 1789 382 635 1451
Fragments arranged by size:
1789 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3948 4346 4620 4767 5178 3948
Size: 398 274 147 411 4285
Fragments arranged by size:
4285 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2478 2508 1178
Size: 30 165 30 1075 30 4185
Fragments arranged by size:
4185 1075 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2072 2290 1186
Size: 886 218 4411
Fragments arranged by size:
4411 886 218
BseYI C'CCAG_C
Cuts at: 4718 5214 4718
Size: 496 5019
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4942
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2965 3726 4754 1540
Size: 275 186 964 761 1028 2301
Fragments arranged by size:
2301 1028 964 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3576 3661 4732 3576
Size: 85 1071 4359
Fragments arranged by size:
4359 1071 85
BsmI GAATG_Cn'
Cuts at: 3026 3119 3026
Size: 93 5422
BsmBI CGTCTCn'nnnn_
Cuts at: 2448 2448
Size: 5515
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2035 2064 2282 2318 2475 2546 2612
Size: 399 245 29 218 36 157 71 66
Cuts at: 2612 2747 2834 3576 3661 4732 1391
Size: 135 87 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 245 218 157 135
87 85 71 66 36 29
BspEI T'CCGG_A
Cuts at: 5178 5178
Size: 5515
BspHI T'CATG_A
Cuts at: 2254 3404 5134 2254
Size: 1150 1730 2635
Fragments arranged by size:
2635 1730 1150
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2870 844
Size: 2026 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3402 1394
Size: 429 1579 3507
Fragments arranged by size:
3507 1579 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4010 4184 65
Size: 1183 2762 174 1396
Fragments arranged by size:
2762 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2147 2513 4159 4346 1700
Size: 110 337 366 1646 187 2869
Fragments arranged by size:
2869 1646 366 337 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5515
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5515
BssSI C'ACGA_G
Cuts at: 3575 4587 3575
Size: 1012 4503
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5515
BstBI TT'CG_AA
Cuts at: 2775 4319 2775
Size: 1544 3971
BstEII G'GTnAC_C
Cuts at: 1184 2350 1184
Size: 1166 4349
BstYI r'GATC_y
Cuts at: 1972 2260 2614 2621 2845 3212 3597 3614 5066
Size: 288 354 7 224 367 385 17 1452
Cuts at: 5066 5152 5164 5510 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 354 346 288 224
86 17 12 7
BtgI C'CryG_G
Cuts at: 513 1065 2004 2418 513
Size: 552 939 414 3610
Fragments arranged by size:
3610 939 552 414
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4776
Fragments arranged by size:
4776 683 56
BtsI GCAGTG_nn'
Cuts at: 3031 3825 3845 3031
Size: 794 20 4701
Fragments arranged by size:
4701 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5515
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5480
DraI TTT'AAA
Cuts at: 2819 2819
Size: 5515
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2348 4522 809
Size: 507 1032 2174 1802
Fragments arranged by size:
2174 1802 1032 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2172 2376 2408
Size: 54 1425 50 275 18 342 204 32
Cuts at: 2408 2962 3851 5480 8
Size: 554 889 1629 43
Fragments arranged by size:
1629 1425 889 554 342 275 204 54
50 43 32 18
EagI C'GGCC_G
Cuts at: 1537 1812 2962 1537
Size: 275 1150 4090
Fragments arranged by size:
4090 1150 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3445 1353
Size: 2092 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4102 4476 4622 387
Size: 1064 138 2513 374 146 1280
Fragments arranged by size:
2513 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2176 2249 2634 3578 4175 4962 5230
Size: 393 544 73 385 944 597 787 268
Cuts at: 5230 1239
Size: 1524
Fragments arranged by size:
1524 944 787 597 544 393 385 268
73
EcoNI CCTnn'n_nnAGG
Cuts at: 2753 2753
Size: 5515
EcoO109I rG'GnC_Cy
Cuts at: 2032 2109 2593 2032
Size: 77 484 4954
Fragments arranged by size:
4954 484 77
EcoRI G'AATT_C
Cuts at: 2824 3202 2824
Size: 378 5137
FspI TGC'GCA
Cuts at: 4021 4021
Size: 5515
HaeII r_GCGC'y
Cuts at: 1678 2680 4662 1678
Size: 1002 1982 2531
Fragments arranged by size:
2531 1982 1002
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2194 2226 2566 2598 2606
Size: 32 135 32 622 32 340 32 8
Cuts at: 2606 2638 2708 2740 4156 4188 4230 4262 1373
Size: 32 70 32 1416 32 42 32 2626
Fragments arranged by size:
2626 1416 622 340 135 70 42 32
32 32 32 32 32 32 32 8
HincII GTy'rAC
Cuts at: 669 1657 1938 2859 3105 669
Size: 988 281 921 246 3079
Fragments arranged by size:
3079 988 921 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5515
HpaI GTT'AAC
Cuts at: 3105 3105
Size: 5515
KpnI G_GTAC'C
Cuts at: 2467 2840 2467
Size: 373 5142
MfeI C'AATT_G
Cuts at: 3114 3114
Size: 5515
MluI A'CGCG_T
Cuts at: 5391 5391
Size: 5515
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4629 4813 1305
Size: 3324 184 2007
Fragments arranged by size:
3324 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2174 2410 10
Size: 54 1425 343 342 236 3115
Fragments arranged by size:
3115 1425 343 342 236 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2357 3473 3832 3991 518
Size: 648 1191 1116 359 159 2042
Fragments arranged by size:
2042 1191 1116 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2196 2433 2931 3608 4756 5001 1606
Size: 590 237 498 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 590 498 245 237
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4024
Fragments arranged by size:
4024 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5515
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5515
NotI GC'GGCC_GC
Cuts at: 2962 2962
Size: 5515
NspI r_CATG'y
Cuts at: 1132 1229 2873 4418 1132
Size: 97 1644 1545 2229
Fragments arranged by size:
2229 1644 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5515
PciI A'CATG_T
Cuts at: 4414 4414
Size: 5515
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2338 1108
Size: 1230 4285
PmeI GTTT'AAAC
Cuts at: 2819 2819
Size: 5515
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3584 3616 5097 5129 3584
Size: 32 1481 32 3970
Fragments arranged by size:
3970 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2109 2593 2109
Size: 484 5031
PshAI GACnn'nnGTC
Cuts at: 1613 2690 1613
Size: 1077 4438
PsiI TTA'TAA
Cuts at: 3085 3085
Size: 5515
PspOMI G'GGCC_C
Cuts at: 2031 2031
Size: 5515
PstI C_TGCA'G
Cuts at: 830 2201 2676 2867 830
Size: 1371 475 191 3478
Fragments arranged by size:
3478 1371 475 191
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5515
PvuII CAG'CTG
Cuts at: 2196 2196
Size: 5515
SalI G'TCGA_C
Cuts at: 1936 2857 1936
Size: 921 4594
SanDI GG'GwC_CC
Cuts at: 2109 2109
Size: 5515
SbfI CC_TGCA'GG
Cuts at: 2867 2867
Size: 5515
ScaI AGT'ACT
Cuts at: 3763 5475 3763
Size: 1712 3803
SexAI A'CCwGG_T
Cuts at: 2796 2796
Size: 5515
SfcI C'TryA_G
Cuts at: 826 1045 2197 2672 2863 3998 4679 4870 826
Size: 219 1152 475 191 1135 681 191 1471
Fragments arranged by size:
1471 1152 1135 681 475 219 191 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2171 2171
Size: 5515
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5515
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5515
SmaI CCC'GGG
Cuts at: 1872 2107 2842 1872
Size: 235 735 4545
Fragments arranged by size:
4545 735 235
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3619 4520 4782 820
Size: 197 926 1676 901 262 1553
Fragments arranged by size:
1676 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3306 493
Size: 2813 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5515
SphI G_CATG'C
Cuts at: 1132 2873 1132
Size: 1741 3774
SspI AAT'ATT
Cuts at: 5 52 3439 5
Size: 47 3387 2081
Fragments arranged by size:
3387 2081 47
StuI AGG'CCT
Cuts at: 1517 2043 1517
Size: 526 4989
StyI C'CwwG_G
Cuts at: 513 1065 2004 2086 2954 513
Size: 552 939 82 868 3074
Fragments arranged by size:
3074 939 868 552 82
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2702 2743 3542 3559 3712 3897 4342
Size: 1255 1122 41 799 17 153 185 445
Cuts at: 4342 325
Size: 1498
Fragments arranged by size:
1498 1255 1122 799 445 185 153 41
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3761 5473 96
Size: 274 80 33 51 1443 1784 1712 138
Fragments arranged by size:
1784 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2121 2153 2478 2510 5475 5507 2121
Size: 32 325 32 2965 32 2129
Fragments arranged by size:
2965 2129 325 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5515
XbaI T'CTAG_A
Cuts at: 2851 2851
Size: 5515
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5515
XmaI C'CCGG_G
Cuts at: 1870 2105 2840 1870
Size: 235 735 4545
Fragments arranged by size:
4545 735 235
XmnI GAAnn'nnTTC
Cuts at: 3644 3644
Size: 5515
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3323 276
Size: 53 83 186 936 1789 2468
Fragments arranged by size:
2468 1789 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI
SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI
RsrII SacI SacII SapI SfoI SrfI SwaI XcmI