Restriction map for
pFN21AF0156
(Circular) (Six-base) MAPSORT of: pFN21AF0156.seq Check: 9025 from: 1 to: 10279
pFN21AF0156 10279 bp
With 182 enzymes: SgfI *
October 20, 2009 10:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2961 2961
Size: 10279
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6114 7155 8089 278
Size: 53 83 186 936 4578 1041 934 2468
Fragments arranged by size:
4578 2468 1041 936 934 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3201 4313 5340 7622 1937
Size: 1264 1112 1027 2282 4594
Fragments arranged by size:
4594 2282 1264 1112 1027
Acc65I G'GTAC_C
Cuts at: 2301 7600 2301
Size: 5299 4980
AclI AA'CG_TT
Cuts at: 1563 8406 8779 1563
Size: 6843 373 3063
Fragments arranged by size:
6843 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2386 2574 2659 3122 3269 3392 3627 5618
Size: 754 188 85 463 147 123 235 1991
Cuts at: 5618 5934 6748 6769 7015 8342 9726 9994 1632
Size: 316 814 21 246 1327 1384 268 1917
Fragments arranged by size:
1991 1917 1384 1327 814 754 463 316
268 246 235 188 147 123 85 21
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 10082
AflIII A'CryG_T
Cuts at: 1203 9178 10155 1203
Size: 7975 977 1327
Fragments arranged by size:
7975 1327 977
AgeI A'CCGG_T
Cuts at: 9110 9110
Size: 10279
AhdI GACnn_n'nnGTC
Cuts at: 3802 9008 3802
Size: 5206 5073
AleI CACnn'nnGTG
Cuts at: 1166 2295 2969 6557 6917 7097 7426 1166
Size: 1129 674 3588 360 180 329 4019
Fragments arranged by size:
4019 3588 1129 674 360 329 180
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2987 7444 1184
Size: 1803 4457 4019
Fragments arranged by size:
4457 4019 1803
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2000 2032 2000
Size: 32 10247
AlwNI CAG_nnn'CTG
Cuts at: 2381 2836 3394 3404 3736 3905 3952 4435 4459
Size: 455 558 10 332 169 47 483 24
Cuts at: 4459 6607 6792 7219 8022 9594 2381
Size: 2148 185 427 803 1572 3066
Fragments arranged by size:
3066 2148 1572 803 558 483 455 427
332 185 169 47 24 10
ApaI G_GGCC'C
Cuts at: 3261 7247 7286 3261
Size: 3986 39 6254
Fragments arranged by size:
6254 3986 39
ApaLI G'TGCA_C
Cuts at: 3337 8336 9492 3337
Size: 4999 1156 4124
Fragments arranged by size:
4999 4124 1156
ApoI r'AATT_y
Cuts at: 1493 2355 5879 6251 7588 7814 7966 10075 1493
Size: 862 3524 372 1337 226 152 2109 1697
Fragments arranged by size:
3524 2109 1697 1337 862 372 226 152
AseI AT'TA_AT
Cuts at: 160 1032 8833 160
Size: 872 7801 1606
Fragments arranged by size:
7801 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10279
AvaI C'yCGr_G
Cuts at: 1870 1943 4676 6955 7604 1870
Size: 73 2733 2279 649 4545
Fragments arranged by size:
4545 2733 2279 649 73
BamHI G'GATC_C
Cuts at: 3050 4836 7609 7976 3050
Size: 1786 2773 367 5353
Fragments arranged by size:
5353 2773 1786 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2301 3303 3347 3413 6475
Size: 325 217 627 514 1002 44 66 3062
Cuts at: 6475 7160 7600 9055 618
Size: 685 440 1455 1842
Fragments arranged by size:
3062 1842 1455 1002 685 627 514 440
325 217 66 44
BanII G_rGCy'C
Cuts at: 1391 2463 2493 2770 3261 3502 3550 3705 4299
Size: 1072 30 277 491 241 48 155 594
Cuts at: 4299 4985 5857 6936 7247 7286 7469 7598 1391
Size: 686 872 1079 311 39 183 129 4072
Fragments arranged by size:
4072 1079 1072 872 686 594 491 311
277 241 183 155 129 48 39 30
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2425 4321 4353 4374 4415 5160 5530
Size: 971 526 1896 32 21 41 745 370
Cuts at: 5530 6710 7355 928
Size: 1180 645 3852
Fragments arranged by size:
3852 1896 1180 971 745 645 526 370
41 32 21
BbvCI CC'TCA_GC
Cuts at: 2048 2663 3455 3671 3888 4070 4657 5073 6410
Size: 615 792 216 217 182 587 416 1337
Cuts at: 6410 6608 7437 2048
Size: 198 829 4890
Fragments arranged by size:
4890 1337 829 792 615 587 416 217
216 198 182
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6946 6980 8470 8504 1903
Size: 34 5009 34 1490 34 3678
Fragments arranged by size:
5009 3678 1490 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7079 8171 9381 7079
Size: 1092 1210 7977
Fragments arranged by size:
7977 1210 1092
BclI T'GATC_A
Cuts at: 1548 5017 1548
Size: 3469 6810
BfrBI ATG'CAT
Cuts at: 4243 4766 4243
Size: 523 9756
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2567 4681 6570
Size: 107 122 71 631 605 895 2114 1889
Cuts at: 6570 6960 8890 136
Size: 390 1930 1525
Fragments arranged by size:
2114 1930 1889 1525 895 631 605 390
122 107 71
BglII A'GATC_T
Cuts at: 2628 2751 5502 10274 2628
Size: 123 2751 4772 2633
Fragments arranged by size:
4772 2751 2633 123
BlpI GC'TnA_GC
Cuts at: 2492 6935 7696 2492
Size: 4443 761 5075
Fragments arranged by size:
5075 4443 761
Bme1580I G_kGCm'C
Cuts at: 1790 3261 3341 3424 6480 6878 7247 7286 8340
Size: 1471 80 83 3056 398 369 39 1054
Cuts at: 8340 9496 1790
Size: 1156 2573
Fragments arranged by size:
3056 2573 1471 1156 1054 398 369 83
80 39
BmgBI CAC'GTC
Cuts at: 1318 3184 4351 6043 6214 1318
Size: 1866 1167 1692 171 5383
Fragments arranged by size:
5383 1866 1692 1167 171
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2924 4195 5756
Size: 416 53 83 399 24 1507 1271 1561
Cuts at: 5756 7465 8010 8968 442
Size: 1709 545 958 1753
Fragments arranged by size:
1753 1709 1561 1507 1271 958 545 416
399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 10279
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4063 6106 4063
Size: 2043 8236
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2119 2507 2858 3308 3497 3617 3703 3750
Size: 880 388 351 450 189 120 86 47
Cuts at: 3750 3764 3776 4037 4481 4518 4939 5519 6076
Size: 14 12 261 444 37 421 580 557
Cuts at: 6076 8939 1239
Size: 2863 2579
Fragments arranged by size:
2863 2579 880 580 557 450 444 421
388 351 261 189 120 86 47 37
14 12
Bpu10I CC'TnA_GC
Cuts at: 2048 2200 2663 2693 3123 3455 3671 3888 4070
Size: 152 463 30 430 332 216 217 182
Cuts at: 4070 4657 4979 4984 5073 6410 6608 7437 7463
Size: 587 322 5 89 1337 198 829 26
Cuts at: 7463 2048
Size: 4864
Fragments arranged by size:
4864 1337 829 587 463 430 332 322
217 216 198 182 152 89 30 26
5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2708 3715 4538 7497 8404 9269 9567 2708
Size: 1007 823 2959 907 865 298 3420
Fragments arranged by size:
3420 2959 1007 907 865 823 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2082 3635 8942 882
Size: 617 583 1553 5307 2219
Fragments arranged by size:
5307 2219 1553 617 583
BsaAI yAC'GTr
Cuts at: 493 1204 6078 7008 7099 8070 493
Size: 711 4874 930 91 971 2702
Fragments arranged by size:
4874 2702 971 930 711 91
BsaBI GATnn'nnATC
Cuts at: 7310 10273 7310
Size: 2963 7316
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6111 7152 8086 8468
Size: 53 83 186 936 4578 1041 934 382
Cuts at: 8468 9103 275
Size: 635 1451
Fragments arranged by size:
4578 1451 1041 936 934 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 5988 8712 9110 9384 9531 9942 5988
Size: 2724 398 274 147 411 6325
Fragments arranged by size:
6325 2724 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3950 3980 5308 5338 1178
Size: 30 165 30 2547 30 1328 30 6119
Fragments arranged by size:
6119 2547 1328 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3347 3680 3995 4556 5947 6735 1186
Size: 2161 333 315 561 1391 788 4730
Fragments arranged by size:
4730 2161 1391 788 561 333 315
BseYI C'CCAG_C
Cuts at: 2395 2868 3329 3487 4125 4861 5109 5125 5591
Size: 473 461 158 638 736 248 16 466
Cuts at: 5591 5729 6505 6768 7285 9482 9978 2395
Size: 138 776 263 517 2197 496 2696
Fragments arranged by size:
2696 2197 776 736 638 517 496 473
466 461 263 248 158 138 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2228 3066 3918 4436 5089 5129 5267
Size: 573 492 838 852 518 653 40 138
Cuts at: 5267 5416 6236 6248 7045 1163
Size: 149 820 12 797 4397
Fragments arranged by size:
4397 852 838 820 797 653 573 518
492 149 138 40 12
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 7729 8490 9518 1540
Size: 275 186 5728 761 1028 2301
Fragments arranged by size:
5728 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2463 2493 3341 3705 3753 4023 4185 4985 6077
Size: 30 848 364 48 270 162 800 1092
Cuts at: 6077 6936 7186 7469 8340 8425 9496 2463
Size: 859 250 283 871 85 1071 3246
Fragments arranged by size:
3246 1092 1071 871 859 848 800 364
283 270 250 162 85 48 30
BsmI GAATG_Cn'
Cuts at: 2554 4148 7790 7883 2554
Size: 1594 3642 93 4950
Fragments arranged by size:
4950 3642 1594 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3217 3867 4538 6204 7159 3217
Size: 650 671 1666 955 6337
Fragments arranged by size:
6337 1666 955 671 650
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2463 2493 2770 3261 3341 3424 3502
Size: 399 673 30 277 491 80 83 78
Cuts at: 3502 3550 3705 3753 4023 4185 4299 4985 5857
Size: 48 155 48 270 162 114 686 872
Cuts at: 5857 6077 6480 6878 6936 7186 7247 7286 7469
Size: 220 403 398 58 250 61 39 183
Cuts at: 7469 7598 8340 8425 9496 1391
Size: 129 742 85 1071 2174
Fragments arranged by size:
2174 1071 872 742 686 673 491 403
399 398 277 270 250 220 183 162
155 129 114 85 83 80 78 61
58 48 48 39 30
BspEI T'CCGG_A
Cuts at: 5988 9942 5988
Size: 3954 6325
BspHI T'CATG_A
Cuts at: 8168 9898 8168
Size: 1730 8549
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2961 3389 5747 6150 7634 844
Size: 2117 428 2358 403 1484 3489
Fragments arranged by size:
3489 2358 2117 1484 428 403
BsrBI CCG'CTC
Cuts at: 1394 1823 4534 7047 8166 1394
Size: 429 2711 2513 1119 3507
Fragments arranged by size:
3507 2711 2513 1119 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4243 5749 8774 8948 65
Size: 1183 2995 1506 3025 174 1396
Fragments arranged by size:
3025 2995 1506 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 8923 9110 1700
Size: 110 7113 187 2869
Fragments arranged by size:
7113 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2715 3966 96
Size: 2619 1251 6409
Fragments arranged by size:
6409 2619 1251
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 10279
BssSI C'ACGA_G
Cuts at: 5425 8339 9351 5425
Size: 2914 1012 6353
Fragments arranged by size:
6353 2914 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3736 3905 6362 794
Size: 2942 169 2457 4711
Fragments arranged by size:
4711 2942 2457 169
BstBI TT'CG_AA
Cuts at: 9083 9083
Size: 10279
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 10279
BstXI CCAn_nnnn'nTGG
Cuts at: 2155 2185 2804 2974 5971 6916 2155
Size: 30 619 170 2997 945 5518
Fragments arranged by size:
5518 2997 945 619 170 30
BstYI r'GATC_y
Cuts at: 1972 2628 2751 3050 4836 5502 5833 7078 7609
Size: 656 123 299 1786 666 331 1245 531
Cuts at: 7609 7976 8361 8378 9830 9916 9928 10274 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1786 1452 1245 666 656 531 385
367 346 331 299 123 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2324 3594 2324
Size: 1270 9009
BtgI C'CryG_G
Cuts at: 513 1065 4904 5328 5724 5847 7559 513
Size: 552 3839 424 396 123 1712 3233
Fragments arranged by size:
3839 3233 1712 552 424 396 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3878 5476 487
Size: 683 56 2652 1598 5290
Fragments arranged by size:
5290 2652 1598 683 56
BtsI GCAGTG_nn'
Cuts at: 3116 3737 5353 6363 7795 8589 8609 3116
Size: 621 1616 1010 1432 794 20 4786
Fragments arranged by size:
4786 1616 1432 1010 794 621 20
ClaI AT'CG_AT
Cuts at: 1554 4479 1554
Size: 2925 7354
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2623 2658 529
Size: 35 2059 35 8150
Fragments arranged by size:
8150 2059 35 35
DraI TTT'AAA
Cuts at: 4092 5569 5896 7583 4092
Size: 1477 327 1687 6788
Fragments arranged by size:
6788 1687 1477 327
DraIII CAC_nnn'GTG
Cuts at: 4638 5260 6067 4638
Size: 622 807 8850
Fragments arranged by size:
8850 807 622
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5927 7334 9286 809
Size: 507 4611 1407 1952 1802
Fragments arranged by size:
4611 1952 1802 1407 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2062 3435 3522
Size: 54 1425 50 275 18 232 1373 87
Cuts at: 3522 5284 5961 6571 6682 7221 7562 7726 8615
Size: 1762 677 610 111 539 341 164 889
Cuts at: 8615 10244 8
Size: 1629 43
Fragments arranged by size:
1762 1629 1425 1373 889 677 610 539
341 275 232 164 111 87 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 7726 1537
Size: 275 5914 4090
Fragments arranged by size:
5914 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2892 3098 4013 4054 4515 4741 6065 7391
Size: 1539 206 915 41 461 226 1324 1326
Cuts at: 7391 8209 1353
Size: 818 3423
Fragments arranged by size:
3423 1539 1326 1324 915 818 461 226
206 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6045 8866 9240 9386 387
Size: 1064 138 4456 2821 374 146 1280
Fragments arranged by size:
4456 2821 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2461 2491 3703 4983 6934 7467 2461
Size: 30 1212 1280 1951 533 5273
Fragments arranged by size:
5273 1951 1280 1212 533 30
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2119 2386 2507 2574 2659 2858 3122
Size: 393 487 267 121 67 85 199 264
Cuts at: 3122 3269 3308 3392 3497 3617 3627 3703 3750
Size: 147 39 84 105 120 10 76 47
Cuts at: 3750 3764 3776 4037 4481 4518 4939 5519 5618
Size: 14 12 261 444 37 421 580 99
Cuts at: 5618 5934 6076 6748 6769 7015 8342 8939 9726
Size: 316 142 672 21 246 1327 597 787
Cuts at: 9726 9994 1239
Size: 268 1524
Fragments arranged by size:
1524 1327 787 672 597 580 487 444
421 393 316 268 267 264 261 246
199 147 142 121 120 105 99 85
84 76 67 47 39 37 21 14
12 10
EcoNI CCTnn'n_nnAGG
Cuts at: 4831 5483 6974 7277 4831
Size: 652 1491 303 7833
Fragments arranged by size:
7833 1491 652 303
EcoO109I rG'GnC_Cy
Cuts at: 2540 2918 3484 3811 4693 4975 5272 5776 6241
Size: 378 566 327 882 282 297 504 465
Cuts at: 6241 6274 6612 6759 6820 7243 7282 7459 7492
Size: 33 338 147 61 423 39 177 33
Cuts at: 7492 7521 2540
Size: 29 5298
Fragments arranged by size:
5298 882 566 504 465 423 378 338
327 297 282 177 147 61 39 33
33 29
EcoRI G'AATT_C
Cuts at: 7588 7966 7588
Size: 378 9901
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2608 3287 3814 4121 4349 5219 2608
Size: 679 527 307 228 870 7668
Fragments arranged by size:
7668 870 679 527 307 228
FspI TGC'GCA
Cuts at: 8785 8785
Size: 10279
HaeII r_GCGC'y
Cuts at: 1678 9426 1678
Size: 7748 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3788 3820 4574 4606 4706
Size: 32 135 32 2216 32 754 32 100
Cuts at: 4706 4738 5408 5440 5777 5809 8920 8952 8994
Size: 32 670 32 337 32 3111 32 42
Cuts at: 8994 9026 1373
Size: 32 2626
Fragments arranged by size:
3111 2626 2216 754 670 337 135 100
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 7623 7869 669
Size: 988 281 5685 246 3079
Fragments arranged by size:
5685 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 5572 6034 748
Size: 4824 462 4993
Fragments arranged by size:
4993 4824 462
HpaI GTT'AAC
Cuts at: 7869 7869
Size: 10279
KpnI G_GTAC'C
Cuts at: 2305 7604 2305
Size: 5299 4980
MfeI C'AATT_G
Cuts at: 7878 7878
Size: 10279
MluI A'CGCG_T
Cuts at: 10155 10155
Size: 10279
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4239 4417 5388 5611 6837 9393 9577 1305
Size: 2934 178 971 223 1226 2556 184 2007
Fragments arranged by size:
2934 2556 2007 1226 971 223 184 178
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2064 3524 5286 6573 6684
Size: 54 1425 343 232 1460 1762 1287 111
Cuts at: 6684 7223 10
Size: 539 3066
Fragments arranged by size:
3066 1762 1460 1425 1287 539 343 232
111 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2036 2295 2955 2969 4028 4188 5969
Size: 648 870 259 660 14 1059 160 1781
Cuts at: 5969 6557 6917 7097 7426 8237 8596 8755 518
Size: 588 360 180 329 811 359 159 2042
Fragments arranged by size:
2042 1781 1059 870 811 660 648 588
360 359 329 259 180 160 159 14
MspA1I CmG'CkG
Cuts at: 1606 2365 2662 3605 4435 5113 5726 6768 6789
Size: 759 297 943 830 678 613 1042 21
Cuts at: 6789 6858 7695 8372 9520 9765 1606
Size: 69 837 677 1148 245 2120
Fragments arranged by size:
2120 1148 1042 943 837 830 759 678
677 613 297 245 69 21
NcoI C'CATG_G
Cuts at: 513 1065 4904 5847 7559 513
Size: 552 3839 943 1712 3233
Fragments arranged by size:
3839 3233 1712 943 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 10279
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 10279
NotI GC'GGCC_GC
Cuts at: 7726 7726
Size: 10279
NsiI A_TGCA'T
Cuts at: 4245 4768 4245
Size: 523 9756
NspI r_CATG'y
Cuts at: 1132 1229 2960 5455 5755 6190 7637 9182 1132
Size: 97 1731 2495 300 435 1447 1545 2229
Fragments arranged by size:
2495 2229 1731 1545 1447 435 300 97
PasI CC'CwG_GG
Cuts at: 1348 3541 5772 6228 7489 1348
Size: 2193 2231 456 1261 4138
Fragments arranged by size:
4138 2231 2193 1261 456
PciI A'CATG_T
Cuts at: 9178 9178
Size: 10279
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2311 2512 2836 3394 3520 3732 3952 4827
Size: 1203 201 324 558 126 212 220 875
Cuts at: 4827 7239 1108
Size: 2412 4148
Fragments arranged by size:
4148 2412 1203 875 558 324 220 212
201 126
PfoI T'CCnGG_A
Cuts at: 3360 3753 4014 4839 5829 3360
Size: 393 261 825 990 7810
Fragments arranged by size:
7810 990 825 393 261
PmeI GTTT'AAAC
Cuts at: 7583 7583
Size: 10279
PmlI CAC'GTG
Cuts at: 6078 7008 7099 6078
Size: 930 91 9258
Fragments arranged by size:
9258 930 91
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 8348 8380 9861 9893 8348
Size: 32 1481 32 8734
Fragments arranged by size:
8734 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2540 3484 3811 4693 5272 5776 6241 6759 6820
Size: 944 327 882 579 504 465 518 61
Cuts at: 6820 2540
Size: 5999
Fragments arranged by size:
5999 944 882 579 518 504 465 327
61
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 10279
PsiI TTA'TAA
Cuts at: 7849 7849
Size: 10279
PspOMI G'GGCC_C
Cuts at: 3257 7243 7282 3257
Size: 3986 39 6254
Fragments arranged by size:
6254 3986 39
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5750 5782 5750
Size: 32 10247
PstI C_TGCA'G
Cuts at: 830 2479 2946 3231 3300 3450 4179 5487 5670
Size: 1649 467 285 69 150 729 1308 183
Cuts at: 5670 6027 6520 6603 7386 7546 7631 830
Size: 357 493 83 783 160 85 3478
Fragments arranged by size:
3478 1649 1308 783 729 493 467 357
285 183 160 150 85 83 69
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 10279
PvuII CAG'CTG
Cuts at: 2365 2662 3605 4435 5113 6768 6789 2365
Size: 297 943 830 678 1655 21 5855
Fragments arranged by size:
5855 1655 943 830 678 297 21
SacI G_AGCT'C
Cuts at: 2463 2493 3705 4985 6936 7469 2463
Size: 30 1212 1280 1951 533 5273
Fragments arranged by size:
5273 1951 1280 1212 533 30
SacII CC_GC'GG
Cuts at: 5727 5727
Size: 10279
SalI G'TCGA_C
Cuts at: 1936 7621 1936
Size: 5685 4594
SanDI GG'GwC_CC
Cuts at: 6241 6241
Size: 10279
SapI GCTCTTCn'nnn_
Cuts at: 3098 6065 3098
Size: 2967 7312
SbfI CC_TGCA'GG
Cuts at: 2946 5487 7631 2946
Size: 2541 2144 5594
Fragments arranged by size:
5594 2541 2144
ScaI AGT'ACT
Cuts at: 8527 10239 8527
Size: 1712 8567
SexAI A'CCwGG_T
Cuts at: 5256 5982 5256
Size: 726 9553
SfcI C'TryA_G
Cuts at: 826 1045 2475 2942 3227 3296 3446 4175 4738
Size: 219 1430 467 285 69 150 729 563
Cuts at: 4738 5483 5666 6023 6516 6592 6599 6717 7382
Size: 745 183 357 493 76 7 118 665
Cuts at: 7382 7474 7542 7627 8762 9443 9634 826
Size: 92 68 85 1135 681 191 1471
Fragments arranged by size:
1471 1430 1135 745 729 681 665 563
493 467 357 285 219 191 183 150
118 92 85 76 69 68 7
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 10279
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 10279
SmaI CCC'GGG
Cuts at: 1872 4678 7606 1872
Size: 2806 2928 4545
Fragments arranged by size:
4545 2928 2806
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2723 3694 4553 7512 8383 9284
Size: 197 926 780 971 859 2959 871 901
Cuts at: 9284 9546 820
Size: 262 1553
Fragments arranged by size:
2959 1553 971 926 901 871 859 780
262 197
SnaBI TAC'GTA
Cuts at: 493 8070 493
Size: 7577 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 10279
SphI G_CATG'C
Cuts at: 1132 2960 6190 7637 1132
Size: 1828 3230 1447 3774
Fragments arranged by size:
3774 3230 1828 1447
SrfI GCCC'GGGC
Cuts at: 4678 4678
Size: 10279
SspI AAT'ATT
Cuts at: 5 52 8203 5
Size: 47 8151 2081
Fragments arranged by size:
8151 2081 47
StuI AGG'CCT
Cuts at: 1517 2336 4559 5564 6011 6980 1517
Size: 819 2223 1005 447 969 4816
Fragments arranged by size:
4816 2223 1005 969 819 447
StyI C'CwwG_G
Cuts at: 513 1065 2531 3519 3645 4689 4904 5847 5862
Size: 552 1466 988 126 1044 215 943 15
Cuts at: 5862 7559 7718 513
Size: 1697 159 3074
Fragments arranged by size:
3074 1697 1466 1044 988 943 552 215
159 126 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2239 2696 3046 3959 5123 5963 6020
Size: 1255 659 457 350 913 1164 840 57
Cuts at: 6020 6519 6591 7109 8306 8323 8476 8661 9106
Size: 499 72 518 1197 17 153 185 445
Cuts at: 9106 325
Size: 1498
Fragments arranged by size:
1498 1255 1197 1164 913 840 659 518
499 457 445 350 185 153 72 57
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2715 2994 3966
Size: 274 80 33 51 1443 738 279 972
Cuts at: 3966 8525 10237 96
Size: 4559 1712 138
Fragments arranged by size:
4559 1712 1443 972 738 279 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3042 3074 3414 3446 3476 3508 3574 3606 4685
Size: 32 340 32 30 32 66 32 1079
Cuts at: 4685 4717 5308 5340 5345 5377 10239 10271 3042
Size: 32 591 32 5 32 4862 32 3050
Fragments arranged by size:
4862 3050 1079 591 340 66 32 32
32 32 32 32 32 32 30 5
Tth111I GACn'n_nGTC
Cuts at: 1316 4064 6290 6818 1316
Size: 2748 2226 528 4777
Fragments arranged by size:
4777 2748 2226 528
XbaI T'CTAG_A
Cuts at: 7615 7615
Size: 10279
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3557 3845 5769 7439 3557
Size: 288 1924 1670 6397
Fragments arranged by size:
6397 1924 1670 288
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 10279
XmaI C'CCGG_G
Cuts at: 1870 4676 7604 1870
Size: 2806 2928 4545
Fragments arranged by size:
4545 2928 2806
XmnI GAAnn'nnTTC
Cuts at: 2520 5825 8408 2520
Size: 3305 2583 4391
Fragments arranged by size:
4391 3305 2583
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6112 7153 8087 276
Size: 53 83 186 936 4578 1041 934 2468
Fragments arranged by size:
4578 2468 1041 936 934 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbeI BsiWI BstZ17I EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI PacI
PspXI RsrII SfiI SfoI SwaI