Restriction map for
pFN21AF0141
(Circular) (Six-base) MAPSORT of: pFN21AF0141.seq Check: 6297 from: 1 to: 8662
pFN21AF0141 8662 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5154 5814 5154
Size: 660 8002
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6472 278
Size: 53 83 186 936 4936 2468
Fragments arranged by size:
4936 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6005 1937
Size: 4068 4594
Acc65I G'GTAC_C
Cuts at: 2857 5983 2857
Size: 3126 5536
AclI AA'CG_TT
Cuts at: 1563 5961 6789 7162 1563
Size: 4398 828 373 3063
Fragments arranged by size:
4398 3063 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2433 3589 5048 5588 5777 6725 8109 8377
Size: 801 1156 1459 540 189 948 1384 268
Cuts at: 8377 1632
Size: 1917
Fragments arranged by size:
1917 1459 1384 1156 948 801 540 268
189
AfeI AGC'GCT
Cuts at: 4046 4885 5690 4046
Size: 839 805 7018
Fragments arranged by size:
7018 839 805
AflII C'TTAA_G
Cuts at: 820 1017 3382 820
Size: 197 2365 6100
Fragments arranged by size:
6100 2365 197
AflIII A'CryG_T
Cuts at: 1203 5271 7561 8538 1203
Size: 4068 2290 977 1327
Fragments arranged by size:
4068 2290 1327 977
AgeI A'CCGG_T
Cuts at: 7493 7493
Size: 8662
AhdI GACnn_n'nnGTC
Cuts at: 5272 7391 5272
Size: 2119 6543
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8662
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4128 1184
Size: 2944 5718
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4415 4447 4415
Size: 32 8630
AlwNI CAG_nnn'CTG
Cuts at: 3017 3110 3170 3844 4174 4225 4492 4642 5401
Size: 93 60 674 330 51 267 150 759
Cuts at: 5401 5473 5936 6405 7977 3017
Size: 72 463 469 1572 3702
Fragments arranged by size:
3702 1572 759 674 469 463 330 267
150 93 72 60 51
ApaI G_GGCC'C
Cuts at: 2237 2466 5102 2237
Size: 229 2636 5797
Fragments arranged by size:
5797 2636 229
ApaLI G'TGCA_C
Cuts at: 6719 7875 6719
Size: 1156 7506
ApoI r'AATT_y
Cuts at: 1493 5971 6197 6349 8458 1493
Size: 4478 226 152 2109 1697
Fragments arranged by size:
4478 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7216 160
Size: 872 6184 1606
Fragments arranged by size:
6184 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8662
AvaI C'yCGr_G
Cuts at: 1870 1943 4239 4614 5104 5987 1870
Size: 73 2296 375 490 883 4545
Fragments arranged by size:
4545 2296 883 490 375 73
BamHI G'GATC_C
Cuts at: 5992 6359 5992
Size: 367 8295
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2279 2723 2857 3586 3709
Size: 325 217 627 492 444 134 729 123
Cuts at: 3709 5983 7438 618
Size: 2274 1455 1842
Fragments arranged by size:
2274 1842 1455 729 627 492 444 325
217 134 123
BanII G_rGCy'C
Cuts at: 1391 2237 2328 2458 2466 2579 2741 3053 3409
Size: 846 91 130 8 113 162 312 356
Cuts at: 3409 3560 3623 3641 4168 4585 5102 5213 5828
Size: 151 63 18 527 417 517 111 615
Cuts at: 5828 5981 1391
Size: 153 4072
Fragments arranged by size:
4072 846 615 527 517 417 356 312
162 153 151 130 113 111 91 63
18 8
BbeI G_GCGC'C
Cuts at: 2727 2727
Size: 8662
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4724 5576 928
Size: 971 2825 852 4014
Fragments arranged by size:
4014 2825 971 852
BbvCI CC'TCA_GC
Cuts at: 5046 5937 5046
Size: 891 7771
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6853 6887 1903
Size: 34 4916 34 3678
Fragments arranged by size:
4916 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6554 7764 6554
Size: 1210 7452
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8662
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2347 3351 3500
Size: 107 122 71 631 605 675 1004 149
Cuts at: 3500 4244 5106 5155 7273 136
Size: 744 862 49 2118 1525
Fragments arranged by size:
2118 1525 1004 862 744 675 631 605
149 122 107 71 49
BglII A'GATC_T
Cuts at: 8657 8657
Size: 8662
BlpI GC'TnA_GC
Cuts at: 2942 3617 4509 4844 5022 5624 6079 2942
Size: 675 892 335 178 602 455 5525
Fragments arranged by size:
5525 892 675 602 455 335 178
Bme1580I G_kGCm'C
Cuts at: 1790 2237 2282 2466 2850 3538 4182 4264 4297
Size: 447 45 184 384 688 644 82 33
Cuts at: 4297 5102 5461 6723 7879 1790
Size: 805 359 1262 1156 2573
Fragments arranged by size:
2573 1262 1156 805 688 644 447 384
359 184 82 45 33
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8662
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2814 3581 3657
Size: 416 53 83 399 24 1397 767 76
Cuts at: 3657 6393 7351 442
Size: 2736 958 1753
Fragments arranged by size:
2736 1753 1397 958 767 416 399 83
76 53 24
BmtI G_CTAG'C
Cuts at: 1056 4148 1056
Size: 3092 5570
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2773 3535 2773
Size: 762 7900
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2201 2232 2652 2725 2745 2857 3147 3182
Size: 962 31 420 73 20 112 290 35
Cuts at: 3182 3216 3676 3794 3866 4115 4418 5225 5423
Size: 34 460 118 72 249 303 807 198
Cuts at: 5423 7322 1239
Size: 1899 2579
Fragments arranged by size:
2579 1899 962 807 460 420 303 290
249 198 118 112 73 72 35 34
31 20
Bpu10I CC'TnA_GC
Cuts at: 4167 4584 5046 5937 4167
Size: 417 462 891 6892
Fragments arranged by size:
6892 891 462 417
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4049 4178 5696 6787 7652 7950 4049
Size: 129 1518 1091 865 298 4761
Fragments arranged by size:
4761 1518 1091 865 298 129
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3842 4946 5219 7325 882
Size: 617 2343 1104 273 2106 2219
Fragments arranged by size:
2343 2219 2106 1104 617 273
BsaAI yAC'GTr
Cuts at: 493 1204 4183 5617 6453 493
Size: 711 2979 1434 836 2702
Fragments arranged by size:
2979 2702 1434 836 711
BsaBI GATnn'nnATC
Cuts at: 8656 8656
Size: 8662
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2724 6469 6851 7486
Size: 53 83 186 936 1191 3745 382 635
Cuts at: 7486 275
Size: 1451
Fragments arranged by size:
3745 1451 1191 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4497 5547 7095 7493 7767 7914 8325 4497
Size: 1050 1548 398 274 147 411 4834
Fragments arranged by size:
4834 1548 1050 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 8437
Fragments arranged by size:
8437 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2639 4016 4085 4478 4481 4801 4804 4819
Size: 1453 1377 69 393 3 320 3 15
Cuts at: 4819 4822 4825 5186 5324 5420 5423 1186
Size: 3 3 361 138 96 3 4425
Fragments arranged by size:
4425 1453 1377 393 361 320 138 96
69 15 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2972 3058 3103 3212 3486 3529 3622 3902 3914
Size: 86 45 109 274 43 93 280 12
Cuts at: 3914 4095 4174 4568 4839 5092 5833 7865 8361
Size: 181 79 394 271 253 741 2032 496
Cuts at: 8361 2972
Size: 3273
Fragments arranged by size:
3273 2032 741 496 394 280 274 271
253 181 109 93 86 79 45 43
12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2081 2296 4096 4613 5597 5835 1163
Size: 573 345 215 1800 517 984 238 3990
Fragments arranged by size:
3990 1800 984 573 517 345 238 215
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6112 6873 7901 1540
Size: 275 186 4111 761 1028 2301
Fragments arranged by size:
4111 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2256 2579 2923 3118 3409 4094 4107 5184 5213
Size: 323 344 195 291 685 13 1077 29
Cuts at: 5213 5625 6723 6808 7879 2256
Size: 412 1098 85 1071 3039
Fragments arranged by size:
3039 1098 1077 1071 685 412 344 323
291 195 85 29 13
BsmI GAATG_Cn'
Cuts at: 3135 4630 6173 6266 3135
Size: 1495 1543 93 5531
Fragments arranged by size:
5531 1543 1495 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5741 5741
Size: 8662
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2237 2256 2282 2328 2458 2466 2579
Size: 399 447 19 26 46 130 8 113
Cuts at: 2579 2741 2850 2923 3053 3118 3409 3538 3560
Size: 162 109 73 130 65 291 129 22
Cuts at: 3560 3623 3641 4094 4107 4168 4182 4264 4297
Size: 63 18 453 13 61 14 82 33
Cuts at: 4297 4585 5102 5184 5213 5461 5625 5828 5981
Size: 288 517 82 29 248 164 203 153
Cuts at: 5981 6723 6808 7879 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 517 453 447 399 291
288 248 203 164 162 153 130 130
129 113 109 85 82 82 73 65
63 61 46 33 29 26 22 19
18 14 13 8
BspEI T'CCGG_A
Cuts at: 4497 5547 8325 4497
Size: 1050 2778 4834
Fragments arranged by size:
4834 2778 1050
BspHI T'CATG_A
Cuts at: 5955 6551 8281 5955
Size: 596 1730 6336
Fragments arranged by size:
6336 1730 596
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2280 2601 2706 2869 5154 5419 5547 5615
Size: 1436 321 105 163 2285 265 128 68
Cuts at: 5615 5814 6017 844
Size: 199 203 3489
Fragments arranged by size:
3489 2285 1436 321 265 203 199 163
128 105 68
BsrBI CCG'CTC
Cuts at: 1394 1823 3244 3727 3802 4162 4258 4291 4447
Size: 429 1421 483 75 360 96 33 156
Cuts at: 4447 4749 5374 5413 6549 1394
Size: 302 625 39 1136 3507
Fragments arranged by size:
3507 1421 1136 625 483 429 360 302
156 96 75 39 33
BsrDI GCAATG_nn'
Cuts at: 65 1248 2311 7157 7331 65
Size: 1183 1063 4846 174 1396
Fragments arranged by size:
4846 1396 1183 1063 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3345 3783 4481 5352 5706 5865 7306
Size: 110 1535 438 698 871 354 159 1441
Cuts at: 7306 7493 1700
Size: 187 2869
Fragments arranged by size:
2869 1535 1441 871 698 438 354 187
159 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8662
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8662
BssSI C'ACGA_G
Cuts at: 3966 6722 7734 3966
Size: 2756 1012 4894
Fragments arranged by size:
4894 2756 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2310 2370 4225 5620 5637 794
Size: 1516 60 1855 1395 17 3819
Fragments arranged by size:
3819 1855 1516 1395 60 17
BstBI TT'CG_AA
Cuts at: 7466 7466
Size: 8662
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8662
BstXI CCAn_nnnn'nTGG
Cuts at: 2734 2734
Size: 8662
BstYI r'GATC_y
Cuts at: 1972 3439 4575 5992 6359 6744 6761 8213 8299
Size: 1467 1136 1417 367 385 17 1452 86
Cuts at: 8299 8311 8657 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1467 1452 1417 1136 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3897 3897
Size: 8662
BtgI C'CryG_G
Cuts at: 513 1065 2004 2055 3496 5593 5641 513
Size: 552 939 51 1441 2097 48 3534
Fragments arranged by size:
3534 2097 1441 939 552 51 48
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4510 487
Size: 683 56 3284 4639
Fragments arranged by size:
4639 3284 683 56
BtsI GCAGTG_nn'
Cuts at: 2149 2254 2800 3244 4262 4295 4326 4937 5818
Size: 105 546 444 1018 33 31 611 881
Cuts at: 5818 6178 6972 6992 2149
Size: 360 794 20 3819
Fragments arranged by size:
3819 1018 881 794 611 546 444 360
105 33 31 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8662
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8627
DraI TTT'AAA
Cuts at: 5966 5966
Size: 8662
DraIII CAC_nnn'GTG
Cuts at: 2131 2822 3575 5620 2131
Size: 691 753 2045 5173
Fragments arranged by size:
5173 2045 753 691
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7669 809
Size: 507 6353 1802
Fragments arranged by size:
6353 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3081 4479 4483
Size: 54 1425 50 275 18 1251 1398 4
Cuts at: 4483 4548 6109 6998 8627 8
Size: 65 1561 889 1629 43
Fragments arranged by size:
1629 1561 1425 1398 1251 889 275 65
54 50 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 6109 1537
Size: 275 4297 4090
Fragments arranged by size:
4297 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2785 4152 4828 4995 6592 1353
Size: 1432 1367 676 167 1597 3423
Fragments arranged by size:
3423 1597 1432 1367 676 167
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3041 4006 5431 7249 7623 7769
Size: 1064 138 1452 965 1425 1818 374 146
Cuts at: 7769 387
Size: 1280
Fragments arranged by size:
1818 1452 1425 1280 1064 965 374 146
138
EcoICRI GAG'CTC
Cuts at: 2577 3407 5211 2577
Size: 830 1804 6028
Fragments arranged by size:
6028 1804 830
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2201 2232 2433 2652 2725 2745 2857
Size: 393 569 31 201 219 73 20 112
Cuts at: 2857 3147 3182 3216 3589 3676 3794 3866 4115
Size: 290 35 34 373 87 118 72 249
Cuts at: 4115 4418 5048 5225 5423 5588 5777 6725 7322
Size: 303 630 177 198 165 189 948 597
Cuts at: 7322 8109 8377 1239
Size: 787 268 1524
Fragments arranged by size:
1524 948 787 630 597 569 393 373
303 290 268 249 219 201 198 189
177 165 118 112 87 73 72 35
34 31 20
EcoNI CCTnn'n_nnAGG
Cuts at: 4736 5405 4736
Size: 669 7993
EcoO109I rG'GnC_Cy
Cuts at: 2033 2069 2233 2463 2540 2590 2837 3352 3893
Size: 36 164 230 77 50 247 515 541
Cuts at: 3893 3960 3981 4393 5099 5398 2033
Size: 67 21 412 706 299 5297
Fragments arranged by size:
5297 706 541 515 412 299 247 230
164 77 67 50 36 21
EcoRI G'AATT_C
Cuts at: 5971 6349 5971
Size: 378 8284
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3619 5575 3619
Size: 1956 6706
FseI GG_CCGG'CC
Cuts at: 4485 4485
Size: 8662
FspI TGC'GCA
Cuts at: 5810 7168 5810
Size: 1358 7304
HaeII r_GCGC'y
Cuts at: 1678 2695 2727 3487 3796 3844 4048 4546 4887
Size: 1017 32 760 309 48 204 498 341
Cuts at: 4887 4963 5692 7809 1678
Size: 76 729 2117 2531
Fragments arranged by size:
2531 2117 1017 760 729 498 341 309
204 76 48 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4567 4599 5258 5290 7303
Size: 32 135 32 2995 32 659 32 2013
Cuts at: 7303 7335 7377 7409 1373
Size: 32 42 32 2626
Fragments arranged by size:
2995 2626 2013 659 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3125 6006 6252 669
Size: 988 281 1187 2881 246 3079
Fragments arranged by size:
3079 2881 1187 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8662
HpaI GTT'AAC
Cuts at: 6252 6252
Size: 8662
KasI G'GCGC_C
Cuts at: 2723 2723
Size: 8662
KpnI G_GTAC'C
Cuts at: 2861 5987 2861
Size: 3126 5536
MfeI C'AATT_G
Cuts at: 6261 6261
Size: 8662
MluI A'CGCG_T
Cuts at: 8538 8538
Size: 8662
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4422 7776 7960 1305
Size: 3117 3354 184 2007
Fragments arranged by size:
3354 3117 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3083 4550 10
Size: 54 1425 343 1251 1467 4122
Fragments arranged by size:
4122 1467 1425 1251 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2732 4110 5187 6620 6979 7138 518
Size: 648 1566 1378 1077 1433 359 159 2042
Fragments arranged by size:
2042 1566 1433 1378 1077 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2179 2854 3070 3418 3696 4225 4330 4639
Size: 573 675 216 348 278 529 105 309
Cuts at: 4639 4797 5008 5045 5096 5473 6078 6755 7903
Size: 158 211 37 51 377 605 677 1148
Cuts at: 7903 8148 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 675 605 573 529 377
348 309 278 245 216 211 158 105
51 37
NaeI GCC'GGC
Cuts at: 3347 4483 3347
Size: 1136 7526
NarI GG'CG_CC
Cuts at: 2724 2724
Size: 8662
NcoI C'CATG_G
Cuts at: 513 1065 2004 2055 3496 5593 513
Size: 552 939 51 1441 2097 3582
Fragments arranged by size:
3582 2097 1441 939 552 51
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8662
NgoMIV G'CCGG_C
Cuts at: 3345 4481 3345
Size: 1136 7526
NheI G'CTAG_C
Cuts at: 1052 4144 1052
Size: 3092 5570
NotI GC'GGCC_GC
Cuts at: 6109 6109
Size: 8662
NspI r_CATG'y
Cuts at: 1132 1229 2368 5275 6020 7565 1132
Size: 97 1139 2907 745 1545 2229
Fragments arranged by size:
2907 2229 1545 1139 745 97
PasI CC'CwG_GG
Cuts at: 1348 2459 4858 1348
Size: 1111 2399 5152
Fragments arranged by size:
5152 2399 1111
PciI A'CATG_T
Cuts at: 5271 7561 5271
Size: 2290 6372
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2056 3110 3277 3844 4441 1108
Size: 948 1054 167 567 597 5329
Fragments arranged by size:
5329 1054 948 597 567 167
PfoI T'CCnGG_A
Cuts at: 3198 3771 3807 4554 3198
Size: 573 36 747 7306
Fragments arranged by size:
7306 747 573 36
PmeI GTTT'AAAC
Cuts at: 5966 5966
Size: 8662
PmlI CAC'GTG
Cuts at: 4183 5617 4183
Size: 1434 7228
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6731 6763 8244 8276 6731
Size: 32 1481 32 7117
Fragments arranged by size:
7117 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2069 2540 2590 2837 3893 2069
Size: 471 50 247 1056 6838
Fragments arranged by size:
6838 1056 471 247 50
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8662
PsiI TTA'TAA
Cuts at: 6232 6232
Size: 8662
PspOMI G'GGCC_C
Cuts at: 2233 2462 5098 2233
Size: 229 2636 5797
Fragments arranged by size:
5797 2636 229
PstI C_TGCA'G
Cuts at: 830 2212 2488 3013 3321 4230 4272 4305 4335
Size: 1382 276 525 308 909 42 33 30
Cuts at: 4335 4944 4977 5448 5896 6014 830
Size: 609 33 471 448 118 3478
Fragments arranged by size:
3478 1382 909 609 525 471 448 308
276 118 42 33 33 30
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8662
PvuII CAG'CTG
Cuts at: 3070 4225 4330 4639 5045 5473 3070
Size: 1155 105 309 406 428 6259
Fragments arranged by size:
6259 1155 428 406 309 105
SacI G_AGCT'C
Cuts at: 2579 3409 5213 2579
Size: 830 1804 6028
Fragments arranged by size:
6028 1804 830
SalI G'TCGA_C
Cuts at: 1936 6004 1936
Size: 4068 4594
SanDI GG'GwC_CC
Cuts at: 2540 2540
Size: 8662
SapI GCTCTTCn'nnn_
Cuts at: 4152 4828 4995 4152
Size: 676 167 7819
Fragments arranged by size:
7819 676 167
SbfI CC_TGCA'GG
Cuts at: 6014 6014
Size: 8662
ScaI AGT'ACT
Cuts at: 6910 8622 6910
Size: 1712 6950
SexAI A'CCwGG_T
Cuts at: 2127 2127
Size: 8662
SfcI C'TryA_G
Cuts at: 826 1045 2208 2228 2470 2484 2891 3009 3317
Size: 219 1163 20 242 14 407 118 308
Cuts at: 3317 3628 4226 4268 4301 4331 4940 4973 5444
Size: 311 598 42 33 30 609 33 471
Cuts at: 5444 5892 6010 7145 7826 8017 826
Size: 448 118 1135 681 191 1471
Fragments arranged by size:
1471 1163 1135 681 609 598 471 448
407 311 308 242 219 191 118 118
42 33 33 30 20 14
SfiI GGCCn_nnn'nGGCC
Cuts at: 3351 3351
Size: 8662
SfoI GGC'GCC
Cuts at: 2725 2725
Size: 8662
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8662
SgrAI Cr'CCGG_yG
Cuts at: 1700 3783 1700
Size: 2083 6579
SmaI CCC'GGG
Cuts at: 1872 4616 5989 1872
Size: 2744 1373 4545
Fragments arranged by size:
4545 2744 1373
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3382 4064 4193 5711 6766 7667
Size: 197 926 1439 682 129 1518 1055 901
Cuts at: 7667 7929 820
Size: 262 1553
Fragments arranged by size:
1553 1518 1439 1055 926 901 682 262
197 129
SnaBI TAC'GTA
Cuts at: 493 6453 493
Size: 5960 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8662
SphI G_CATG'C
Cuts at: 1132 6020 1132
Size: 4888 3774
SspI AAT'ATT
Cuts at: 5 52 6586 5
Size: 47 6534 2081
Fragments arranged by size:
6534 2081 47
StuI AGG'CCT
Cuts at: 1517 2241 2968 3972 4708 1517
Size: 724 727 1004 736 5471
Fragments arranged by size:
5471 1004 736 727 724
StyI C'CwwG_G
Cuts at: 513 1065 2004 2055 2073 3496 4350 5593 6101
Size: 552 939 51 18 1423 854 1243 508
Cuts at: 6101 513
Size: 3074
Fragments arranged by size:
3074 1423 1243 939 854 552 508 51
18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2833 3050 3563 3580 4034 4109 4910
Size: 1255 1253 217 513 17 454 75 801
Cuts at: 4910 6689 6706 6859 7044 7489 325
Size: 1779 17 153 185 445 1498
Fragments arranged by size:
1779 1498 1255 1253 801 513 454 445
217 185 153 75 17 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5793 5871 6908
Size: 274 80 33 51 1443 3816 78 1037
Cuts at: 6908 8620 96
Size: 1712 138
Fragments arranged by size:
3816 1712 1443 1037 274 138 80 78
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2829 2861 3121 3153 3424 3456 4977 5009 8622
Size: 32 260 32 271 32 1521 32 3613
Cuts at: 8622 8654 2829
Size: 32 2837
Fragments arranged by size:
3613 2837 1521 271 260 32 32 32
32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8662
XbaI T'CTAG_A
Cuts at: 5998 5998
Size: 8662
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2033 2881 3937 2033
Size: 848 1056 6758
Fragments arranged by size:
6758 1056 848
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8662
XmaI C'CCGG_G
Cuts at: 1870 4614 5987 1870
Size: 2744 1373 4545
Fragments arranged by size:
4545 2744 1373
XmnI GAAnn'nnTTC
Cuts at: 3289 6791 3289
Size: 3502 5160
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6470 276
Size: 53 83 186 936 4936 2468
Fragments arranged by size:
4936 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV FspAI
NruI NsiI PacI PspXI PsrI RsrII SacII SrfI
SwaI