Restriction map for
pFN21AF0120
(Circular) (Six-base) MAPSORT of: pFN21AF0120.seq Check: 8063 from: 1 to: 8167
pFN21AF0120 8167 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5977 278
Size: 53 83 186 936 4441 2468
Fragments arranged by size:
4441 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4461 4578 5510 1937
Size: 2524 117 932 4594
Fragments arranged by size:
4594 2524 932 117
Acc65I G'GTAC_C
Cuts at: 4275 5488 4275
Size: 1213 6954
AclI AA'CG_TT
Cuts at: 1563 6294 6667 1563
Size: 4731 373 3063
Fragments arranged by size:
4731 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2069 2783 2852 3477 3530 3572 4409 4688
Size: 437 714 69 625 53 42 837 279
Cuts at: 4688 5339 6230 7614 7882 1632
Size: 651 891 1384 268 1917
Fragments arranged by size:
1917 1384 891 837 714 651 625 437
279 268 69 53 42
AfeI AGC'GCT
Cuts at: 4635 4635
Size: 8167
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7970
AflIII A'CryG_T
Cuts at: 1203 5100 5373 7066 8043 1203
Size: 3897 273 1693 977 1327
Fragments arranged by size:
3897 1693 1327 977 273
AgeI A'CCGG_T
Cuts at: 2706 6998 2706
Size: 4292 3875
AhdI GACnn_n'nnGTC
Cuts at: 3414 6896 3414
Size: 3482 4685
AleI CACnn'nnGTG
Cuts at: 1166 2357 3968 4413 1166
Size: 1191 1611 445 4920
Fragments arranged by size:
4920 1611 1191 445
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3539 1184
Size: 2355 5812
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5282 5314 5282
Size: 32 8135
AlwNI CAG_nnn'CTG
Cuts at: 2803 2851 2959 3349 4141 4549 4978 5910 7482
Size: 48 108 390 792 408 429 932 1572
Cuts at: 7482 2803
Size: 3488
Fragments arranged by size:
3488 1572 932 792 429 408 390 108
48
ApaI G_GGCC'C
Cuts at: 3989 3996 3989
Size: 7 8160
ApaLI G'TGCA_C
Cuts at: 4079 4406 6224 7380 4079
Size: 327 1818 1156 4866
Fragments arranged by size:
4866 1818 1156 327
ApoI r'AATT_y
Cuts at: 1493 2291 5476 5702 5854 7963 1493
Size: 798 3185 226 152 2109 1697
Fragments arranged by size:
3185 2109 1697 798 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6721 160
Size: 872 5689 1606
Fragments arranged by size:
5689 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8167
AvaI C'yCGr_G
Cuts at: 1870 1943 2684 3159 3706 4653 4771 4987 5082
Size: 73 741 475 547 947 118 216 95
Cuts at: 5082 5492 1870
Size: 410 4545
Fragments arranged by size:
4545 947 741 547 475 410 216 118
95 73
BamHI G'GATC_C
Cuts at: 5497 5864 5497
Size: 367 7800
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3312 4275 4493 4583 4994
Size: 325 217 627 1525 963 218 90 411
Cuts at: 4994 5488 6943 618
Size: 494 1455 1842
Fragments arranged by size:
1842 1525 1455 963 627 494 411 325
218 217 90
BanII G_rGCy'C
Cuts at: 1391 2337 3051 3705 3989 3996 4519 5486 1391
Size: 946 714 654 284 7 523 967 4072
Fragments arranged by size:
4072 967 946 714 654 523 284 7
BbeI G_GCGC'C
Cuts at: 3316 3316
Size: 8167
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2266 2840 3465 3777 5066 928
Size: 971 367 574 625 312 1289 4029
Fragments arranged by size:
4029 1289 971 625 574 367 312
BbvCI CC'TCA_GC
Cuts at: 4877 4877
Size: 8167
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3745 3779 6358 6392 1903
Size: 34 50 34 1724 34 2579 34 3678
Fragments arranged by size:
3678 2579 1724 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6059 7269 6059
Size: 1210 6957
BclI T'GATC_A
Cuts at: 1548 4311 5196 1548
Size: 2763 885 4519
Fragments arranged by size:
4519 2763 885
BfrBI ATG'CAT
Cuts at: 5112 5112
Size: 8167
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2379 2999 6778
Size: 107 122 71 631 605 707 620 3779
Cuts at: 6778 136
Size: 1525
Fragments arranged by size:
3779 1525 707 631 620 605 122 107
71
BglII A'GATC_T
Cuts at: 3802 8162 3802
Size: 4360 3807
BlpI GC'TnA_GC
Cuts at: 2102 5584 2102
Size: 3482 4685
Bme1580I G_kGCm'C
Cuts at: 1790 3585 3978 3989 3996 4083 4410 4496 4606
Size: 1795 393 11 7 87 327 86 110
Cuts at: 4606 6228 7384 1790
Size: 1622 1156 2573
Fragments arranged by size:
2573 1795 1622 1156 393 327 110 87
86 11 7
BmgBI CAC'GTC
Cuts at: 1318 4411 1318
Size: 3093 5074
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2334 5898 6856
Size: 416 53 83 399 24 917 3564 958
Cuts at: 6856 442
Size: 1753
Fragments arranged by size:
3564 1753 958 917 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8167
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2501 2759 2831 2963 3047 3065 3140 3254
Size: 1262 258 72 132 84 18 75 114
Cuts at: 3254 3317 3923 4342 5186 5354 6827 1239
Size: 63 606 419 844 168 1473 2579
Fragments arranged by size:
2579 1473 1262 844 606 419 258 168
132 114 84 75 72 63 18
Bpu10I CC'TnA_GC
Cuts at: 4877 4877
Size: 8167
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3035 6292 7157 7455 3035
Size: 3257 865 298 3747
Fragments arranged by size:
3747 3257 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2667 5060 6830 882
Size: 617 1168 2393 1770 2219
Fragments arranged by size:
2393 2219 1770 1168 617
BsaAI yAC'GTr
Cuts at: 493 1204 3979 5958 493
Size: 711 2775 1979 2702
Fragments arranged by size:
2775 2702 1979 711
BsaBI GATnn'nnATC
Cuts at: 3257 8161 3257
Size: 4904 3263
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2064 3313 3603 3795
Size: 53 83 186 936 531 1249 290 192
Cuts at: 3795 4371 4812 5974 6356 6991 275
Size: 576 441 1162 382 635 1451
Fragments arranged by size:
1451 1249 1162 936 635 576 531 441
382 290 192 186 83 53
BsaWI w'CCGG_w
Cuts at: 2706 4278 4608 6600 6998 7272 7419 7830 2706
Size: 1572 330 1992 398 274 147 411 3043
Fragments arranged by size:
3043 1992 1572 411 398 330 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2840 2870 3038 3068 3845
Size: 30 165 30 1437 30 168 30 777
Cuts at: 3845 3875 5282 5312 1178
Size: 30 1407 30 4033
Fragments arranged by size:
4033 1437 1407 777 168 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2028 2657 2680 2738 3002 3020 3044 3137
Size: 842 629 23 58 264 18 24 93
Cuts at: 3137 3251 3461 3835 3847 3850 3853 3856 4517
Size: 114 210 374 12 3 3 3 661
Cuts at: 4517 4586 5306 5309 5405 5441 1186
Size: 69 720 3 96 36 3912
Fragments arranged by size:
3912 842 720 661 629 374 264 210
114 96 93 69 58 36 24 23
18 12 3 3 3 3
BseYI C'CCAG_C
Cuts at: 3492 4038 4051 4502 4518 4864 5171 7370 7866
Size: 546 13 451 16 346 307 2199 496
Cuts at: 7866 3492
Size: 3793
Fragments arranged by size:
3793 2199 546 496 451 346 307 16
13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4022 4235 4252 4360 4991 5167 1163
Size: 573 2286 213 17 108 631 176 4163
Fragments arranged by size:
4163 2286 631 573 213 176 108 17
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2929 5617 6378 7406 1540
Size: 275 186 928 2688 761 1028 2301
Fragments arranged by size:
2688 2301 1028 928 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3051 3059 4083 4410 4938 5226 6228 6313 7384
Size: 8 1024 327 528 288 1002 85 1071
Cuts at: 7384 3051
Size: 3834
Fragments arranged by size:
3834 1071 1024 1002 528 327 288 85
8
BsmI GAATG_Cn'
Cuts at: 5114 5678 5771 5114
Size: 564 93 7510
Fragments arranged by size:
7510 564 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2036 4673 5279 2036
Size: 2637 606 4924
Fragments arranged by size:
4924 2637 606
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2337 3051 3059 3585 3705 3978 3989
Size: 399 547 714 8 526 120 273 11
Cuts at: 3989 3996 4083 4410 4496 4519 4606 4938 5226
Size: 7 87 327 86 23 87 332 288
Cuts at: 5226 5486 6228 6313 7384 1391
Size: 260 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 714 547 526 399 332
327 288 273 260 120 87 87 86
85 23 11 8 7
BspEI T'CCGG_A
Cuts at: 4608 7830 4608
Size: 3222 4945
BspHI T'CATG_A
Cuts at: 2661 6056 7786 2661
Size: 3395 1730 3042
Fragments arranged by size:
3395 3042 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2148 2451 2788 2988 3369 3898 4707 5522
Size: 1304 303 337 200 381 529 809 815
Cuts at: 5522 844
Size: 3489
Fragments arranged by size:
3489 1304 815 809 529 381 337 303
200
BsrBI CCG'CTC
Cuts at: 1394 1823 2539 2563 2815 3133 3139 4756 6054
Size: 429 716 24 252 318 6 1617 1298
Cuts at: 6054 1394
Size: 3507
Fragments arranged by size:
3507 1617 1298 716 429 318 252 24
6
BsrDI GCAATG_nn'
Cuts at: 65 1248 6662 6836 65
Size: 1183 5414 174 1396
Fragments arranged by size:
5414 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2091 2226 2706 4115 4926 5313 6811
Size: 110 281 135 480 1409 811 387 1498
Cuts at: 6811 6998 1700
Size: 187 2869
Fragments arranged by size:
2869 1498 1409 811 480 387 281 187
135 110
BsrGI T'GTAC_A
Cuts at: 96 2310 3084 96
Size: 2214 774 5179
Fragments arranged by size:
5179 2214 774
BssHII G'CGCG_C
Cuts at: 1925 4452 1925
Size: 2527 5640
BssSI C'ACGA_G
Cuts at: 2375 3584 4439 6227 7239 2375
Size: 1209 855 1788 1012 3303
Fragments arranged by size:
3303 1788 1209 1012 855
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4978 5161 794
Size: 4184 183 3800
Fragments arranged by size:
4184 3800 183
BstBI TT'CG_AA
Cuts at: 5106 6971 5106
Size: 1865 6302
BstEII G'GTnAC_C
Cuts at: 1184 3961 1184
Size: 2777 5390
BstXI CCAn_nnnn'nTGG
Cuts at: 2359 2362 4366 4954 4966 5449 2359
Size: 3 2004 588 12 483 5077
Fragments arranged by size:
5077 2004 588 483 12 3
BstYI r'GATC_y
Cuts at: 1972 2970 3222 3623 3802 5497 5864 6249 6266
Size: 998 252 401 179 1695 367 385 17
Cuts at: 6266 7718 7804 7816 8162 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1695 1452 998 401 385 367 346
252 179 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 4998 5358 513
Size: 552 3933 360 3322
Fragments arranged by size:
3933 3322 552 360
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3737 5098 487
Size: 683 56 2511 1361 3556
Fragments arranged by size:
3556 2511 1361 683 56
BtsI GCAGTG_nn'
Cuts at: 3194 5155 5683 6477 6497 3194
Size: 1961 528 794 20 4864
Fragments arranged by size:
4864 1961 794 528 20
ClaI AT'CG_AT
Cuts at: 1554 2691 1554
Size: 1137 7030
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8132
DraI TTT'AAA
Cuts at: 5471 5471
Size: 8167
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2585 3722 4655 7174 809
Size: 507 1269 1137 933 2519 1802
Fragments arranged by size:
2519 1802 1269 1137 933 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2463 4104 4693
Size: 54 1425 50 275 18 633 1641 589
Cuts at: 4693 4956 5139 5614 6503 8132 8
Size: 263 183 475 889 1629 43
Fragments arranged by size:
1641 1629 1425 889 633 589 475 275
263 183 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5614 1537
Size: 275 3802 4090
Fragments arranged by size:
4090 3802 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2052 2553 4836 4943 5231 6097 1353
Size: 699 501 2283 107 288 866 3423
Fragments arranged by size:
3423 2283 866 699 501 288 107
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3477 6754 7128 7274 387
Size: 1064 138 1888 3277 374 146 1280
Fragments arranged by size:
3277 1888 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3049 3049
Size: 8167
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2069 2501 2759 2783 2831 2852 2963
Size: 393 437 432 258 24 48 21 111
Cuts at: 2963 3047 3065 3140 3254 3317 3477 3530 3572
Size: 84 18 75 114 63 160 53 42
Cuts at: 3572 3923 4342 4409 4688 5186 5339 5354 6230
Size: 351 419 67 279 498 153 15 876
Cuts at: 6230 6827 7614 7882 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 876 787 597 498 437 432 419
393 351 279 268 258 160 153 114
111 84 75 67 63 53 48 42
24 21 18 15
EcoNI CCTnn'n_nnAGG
Cuts at: 2984 3068 3320 3905 4124 5165 2984
Size: 84 252 585 219 1041 5986
Fragments arranged by size:
5986 1041 585 252 219 84
EcoO109I rG'GnC_Cy
Cuts at: 2380 2440 2938 3040 3883 3985 3992 3993 5338
Size: 60 498 102 843 102 7 1 1345
Cuts at: 5338 2380
Size: 5209
Fragments arranged by size:
5209 1345 843 498 102 102 60 7
1
EcoRI G'AATT_C
Cuts at: 5476 5854 5476
Size: 378 7789
EcoRV GAT'ATC
Cuts at: 5026 5026
Size: 8167
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4652 4652
Size: 8167
FspI TGC'GCA
Cuts at: 6673 6673
Size: 8167
HaeII r_GCGC'y
Cuts at: 1678 3316 4549 4637 4924 7314 1678
Size: 1638 1233 88 287 2390 2531
Fragments arranged by size:
2531 2390 1638 1233 287 88
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2840 2872 3400 3432 5181
Size: 32 135 32 1268 32 528 32 1749
Cuts at: 5181 5213 6808 6840 6882 6914 1373
Size: 32 1595 32 42 32 2626
Fragments arranged by size:
2626 1749 1595 1268 528 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2965 3292 3913 4264 4462 5511
Size: 988 281 1027 327 621 351 198 1049
Cuts at: 5511 5757 669
Size: 246 3079
Fragments arranged by size:
3079 1049 1027 988 621 351 327 281
246 198
HindIII A'AGCT_T
Cuts at: 748 4681 748
Size: 3933 4234
HpaI GTT'AAC
Cuts at: 5757 5757
Size: 8167
KasI G'GCGC_C
Cuts at: 3312 3312
Size: 8167
KpnI G_GTAC'C
Cuts at: 4279 5492 4279
Size: 1213 6954
MfeI C'AATT_G
Cuts at: 5766 5766
Size: 8167
MluI A'CGCG_T
Cuts at: 5100 8043 5100
Size: 2943 5224
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2214 2704 4167 5229 7281 7465 1305
Size: 909 490 1463 1062 2052 184 2007
Fragments arranged by size:
2052 2007 1463 1062 909 490 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2465 4695 4958 10
Size: 54 1425 343 633 2230 263 3219
Fragments arranged by size:
3219 2230 1425 633 343 263 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2357 2360 3107 3653 3968 4413 5234
Size: 648 1191 3 747 546 315 445 821
Cuts at: 5234 5447 6125 6484 6643 518
Size: 213 678 359 159 2042
Fragments arranged by size:
2042 1191 821 747 678 648 546 445
359 315 213 159 3
MspA1I CmG'CkG
Cuts at: 1606 2479 3118 3523 3783 4138 4236 4366 5464
Size: 873 639 405 260 355 98 130 1098
Cuts at: 5464 5583 6260 7408 7653 1606
Size: 119 677 1148 245 2120
Fragments arranged by size:
2120 1148 1098 873 677 639 405 355
260 245 130 119 98
NaeI GCC'GGC
Cuts at: 2228 4117 4928 5315 2228
Size: 1889 811 387 5080
Fragments arranged by size:
5080 1889 811 387
NarI GG'CG_CC
Cuts at: 3313 3313
Size: 8167
NcoI C'CATG_G
Cuts at: 513 1065 4998 513
Size: 552 3933 3682
Fragments arranged by size:
3933 3682 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8167
NgoMIV G'CCGG_C
Cuts at: 2226 4115 4926 5313 2226
Size: 1889 811 387 5080
Fragments arranged by size:
5080 1889 811 387
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8167
NotI GC'GGCC_GC
Cuts at: 5614 5614
Size: 8167
NruI TCG'CGA
Cuts at: 4675 4675
Size: 8167
NsiI A_TGCA'T
Cuts at: 5114 5114
Size: 8167
NspI r_CATG'y
Cuts at: 1132 1229 2101 4197 5377 5525 7070 1132
Size: 97 872 2096 1180 148 1545 2229
Fragments arranged by size:
2229 2096 1545 1180 872 148 97
PasI CC'CwG_GG
Cuts at: 1348 3064 3989 1348
Size: 1716 925 5526
Fragments arranged by size:
5526 1716 925
PciI A'CATG_T
Cuts at: 5373 7066 5373
Size: 1693 6474
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4483 1108
Size: 3375 4792
PfoI T'CCnGG_A
Cuts at: 2532 2907 2532
Size: 375 7792
PmeI GTTT'AAAC
Cuts at: 5471 5471
Size: 8167
PmlI CAC'GTG
Cuts at: 3979 3979
Size: 8167
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5282 5314 6236 6268 7749 7781 5282
Size: 32 922 32 1481 32 5668
Fragments arranged by size:
5668 1481 922 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2440 3040 2440
Size: 600 7567
PshAI GACnn'nnGTC
Cuts at: 1613 3345 1613
Size: 1732 6435
PsiI TTA'TAA
Cuts at: 5737 5737
Size: 8167
PspOMI G'GGCC_C
Cuts at: 3985 3992 3985
Size: 7 8160
PstI C_TGCA'G
Cuts at: 830 2421 2856 2985 3117 3528 4545 4623 5519
Size: 1591 435 129 132 411 1017 78 896
Cuts at: 5519 830
Size: 3478
Fragments arranged by size:
3478 1591 1017 896 435 411 132 129
78
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8167
PvuII CAG'CTG
Cuts at: 2479 3118 3523 4138 4366 5464 2479
Size: 639 405 615 228 1098 5182
Fragments arranged by size:
5182 1098 639 615 405 228
SacI G_AGCT'C
Cuts at: 3051 3051
Size: 8167
SalI G'TCGA_C
Cuts at: 1936 4460 5509 1936
Size: 2524 1049 4594
Fragments arranged by size:
4594 2524 1049
SapI GCTCTTCn'nnn_
Cuts at: 2553 4836 4943 5231 2553
Size: 2283 107 288 5489
Fragments arranged by size:
5489 2283 288 107
SbfI CC_TGCA'GG
Cuts at: 2985 5519 2985
Size: 2534 5633
ScaI AGT'ACT
Cuts at: 3182 3470 6415 8127 3182
Size: 288 2945 1712 3222
Fragments arranged by size:
3222 2945 1712 288
SfcI C'TryA_G
Cuts at: 826 1045 2417 2852 2981 3113 3524 4507 4541
Size: 219 1372 435 129 132 411 983 34
Cuts at: 4541 4619 4891 5515 6650 7331 7522 826
Size: 78 272 624 1135 681 191 1471
Fragments arranged by size:
1471 1372 1135 983 681 624 435 411
272 219 191 132 129 78 34
SfiI GGCCn_nnn'nGGCC
Cuts at: 2379 2379
Size: 8167
SfoI GGC'GCC
Cuts at: 3314 3314
Size: 8167
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8167
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8167
SmaI CCC'GGG
Cuts at: 1872 3708 4655 5494 1872
Size: 1836 947 839 4545
Fragments arranged by size:
4545 1836 947 839
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2684 3050 6271 7172 7434 820
Size: 197 926 741 366 3221 901 262 1553
Fragments arranged by size:
3221 1553 926 901 741 366 262 197
SnaBI TAC'GTA
Cuts at: 493 5958 493
Size: 5465 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8167
SphI G_CATG'C
Cuts at: 1132 5525 1132
Size: 4393 3774
SspI AAT'ATT
Cuts at: 5 52 6091 5
Size: 47 6039 2081
Fragments arranged by size:
6039 2081 47
StuI AGG'CCT
Cuts at: 1517 3149 3281 1517
Size: 1632 132 6403
Fragments arranged by size:
6403 1632 132
StyI C'CwwG_G
Cuts at: 513 1065 2250 2436 3321 3576 4998 5606 513
Size: 552 1185 186 885 255 1422 608 3074
Fragments arranged by size:
3074 1422 1185 885 608 552 255 186
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3949 4075 4601 4960 5437 6194 6211
Size: 1255 2369 126 526 359 477 757 17
Cuts at: 6211 6364 6549 6994 325
Size: 153 185 445 1498
Fragments arranged by size:
2369 1498 1255 757 526 477 445 359
185 153 126 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2310 3084 3180
Size: 274 80 33 51 1443 333 774 96
Cuts at: 3180 3210 3468 6413 8125 96
Size: 30 258 2945 1712 138
Fragments arranged by size:
2945 1712 1443 774 333 274 258 138
96 80 51 33 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2054 2086 3038 3070 3845 3877 8127 8159 2054
Size: 32 952 32 775 32 4250 32 2062
Fragments arranged by size:
4250 2062 952 775 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8167
XbaI T'CTAG_A
Cuts at: 5503 5503
Size: 8167
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2359 2359
Size: 8167
XhoI C'TCGA_G
Cuts at: 1943 2684 1943
Size: 741 7426
XmaI C'CCGG_G
Cuts at: 1870 3706 4653 5492 1870
Size: 1836 947 839 4545
Fragments arranged by size:
4545 1836 947 839
XmnI GAAnn'nnTTC
Cuts at: 6296 6296
Size: 8167
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5975 276
Size: 53 83 186 936 4441 2468
Fragments arranged by size:
4441 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SalI SapI SbfI
ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BplI BsiWI BstZ17I Bsu36I
DraIII FseI FspAI PacI PspXI PsrI RsrII SacII
SanDI SexAI SrfI SwaI