Restriction map for
pFN21AF0040
(Circular) (Six-base) MAPSORT of: pFN21AF0040.seq Check: 8769 from: 1 to: 7855
pFN21AF0040 7855 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4304 4304
Size: 7855
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5665 278
Size: 53 83 186 936 4129 2468
Fragments arranged by size:
4129 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2150 4644 5198 1937
Size: 213 2494 554 4594
Fragments arranged by size:
4594 2494 554 213
Acc65I G'GTAC_C
Cuts at: 4690 5176 4690
Size: 486 7369
AclI AA'CG_TT
Cuts at: 1563 5982 6355 1563
Size: 4419 373 3063
Fragments arranged by size:
4419 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2735 3001 3815 4316 4667 5918 7302 7570
Size: 1103 266 814 501 351 1251 1384 268
Cuts at: 7570 1632
Size: 1917
Fragments arranged by size:
1917 1384 1251 1103 814 501 351 268
266
AfeI AGC'GCT
Cuts at: 3914 4542 3914
Size: 628 7227
AflII C'TTAA_G
Cuts at: 820 1017 3152 820
Size: 197 2135 5523
Fragments arranged by size:
5523 2135 197
AflIII A'CryG_T
Cuts at: 1203 6754 7731 1203
Size: 5551 977 1327
Fragments arranged by size:
5551 1327 977
AgeI A'CCGG_T
Cuts at: 6686 6686
Size: 7855
AhdI GACnn_n'nnGTC
Cuts at: 3664 6584 3664
Size: 2920 4935
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7855
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7855
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3755 3787 4593 4625 3755
Size: 32 806 32 6985
Fragments arranged by size:
6985 806 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2572 4037 4618 5007 5598 7170 2572
Size: 1465 581 389 591 1572 3257
Fragments arranged by size:
3257 1572 1465 591 581 389
ApaI G_GGCC'C
Cuts at: 5102 5102
Size: 7855
ApaLI G'TGCA_C
Cuts at: 5912 7068 5912
Size: 1156 6699
ApoI r'AATT_y
Cuts at: 1493 2661 2995 4130 4660 5164 5390 5542 7651
Size: 1168 334 1135 530 504 226 152 2109
Cuts at: 7651 1493
Size: 1697
Fragments arranged by size:
2109 1697 1168 1135 530 504 334 226
152
AseI AT'TA_AT
Cuts at: 160 1032 6409 160
Size: 872 5377 1606
Fragments arranged by size:
5377 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7855
AvaI C'yCGr_G
Cuts at: 1870 1943 5180 1870
Size: 73 3237 4545
Fragments arranged by size:
4545 3237 73
BamHI G'GATC_C
Cuts at: 5185 5552 5185
Size: 367 7488
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2386 3461 4316 4690 5176
Size: 325 217 627 599 1075 855 374 486
Cuts at: 5176 6631 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 1075 855 627 599 486 374
325 217
BanII G_rGCy'C
Cuts at: 1391 5102 5174 1391
Size: 3711 72 4072
Fragments arranged by size:
4072 3711 72
BbeI G_GCGC'C
Cuts at: 3465 3465
Size: 7855
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2028 2261 2449 4564 928
Size: 971 129 233 188 2115 4219
Fragments arranged by size:
4219 2115 971 233 188 129
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6046 6080 1903
Size: 34 4109 34 3678
Fragments arranged by size:
4109 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3044 5747 6957 3044
Size: 2703 1210 3942
Fragments arranged by size:
3942 2703 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7855
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4233 4365 4930
Size: 107 122 71 631 605 2561 132 565
Cuts at: 4930 5017 6466 136
Size: 87 1449 1525
Fragments arranged by size:
2561 1525 1449 631 605 565 132 122
107 87 71
BglII A'GATC_T
Cuts at: 2746 4045 7850 2746
Size: 1299 3805 2751
Fragments arranged by size:
3805 2751 1299
BlpI GC'TnA_GC
Cuts at: 2820 5272 2820
Size: 2452 5403
Bme1580I G_kGCm'C
Cuts at: 1790 2283 3396 4157 4168 5102 5916 7072 1790
Size: 493 1113 761 11 934 814 1156 2573
Fragments arranged by size:
2573 1156 1113 934 814 761 493 11
BmgBI CAC'GTC
Cuts at: 1318 3661 4570 1318
Size: 2343 909 4603
Fragments arranged by size:
4603 2343 909
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3056 5586 6544
Size: 416 53 83 399 24 1639 2530 958
Cuts at: 6544 442
Size: 1753
Fragments arranged by size:
2530 1753 1639 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7855
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2598 4995 2598
Size: 2397 5458
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2546 2936 3361 4112 6515 1239
Size: 1307 390 425 751 2403 2579
Fragments arranged by size:
2579 2403 1307 751 425 390
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3794 3961 5980 6845 7143 3794
Size: 167 2019 865 298 4506
Fragments arranged by size:
4506 2019 865 298 167
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2912 3983 6518 882
Size: 617 1413 1071 2535 2219
Fragments arranged by size:
2535 2219 1413 1071 617
BsaAI yAC'GTr
Cuts at: 493 1204 2686 5646 493
Size: 711 1482 2960 2702
Fragments arranged by size:
2960 2702 1482 711
BsaBI GATnn'nnATC
Cuts at: 4044 7849 4044
Size: 3805 4050
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3462 3741 3803 5662
Size: 53 83 186 936 1929 279 62 1859
Cuts at: 5662 6044 6679 275
Size: 382 635 1451
Fragments arranged by size:
1929 1859 1451 936 635 382 279 186
83 62 53
BsaWI w'CCGG_w
Cuts at: 2776 4460 6288 6686 6960 7107 7518 2776
Size: 1684 1828 398 274 147 411 3113
Fragments arranged by size:
3113 1828 1684 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2878 2908 2909 2939 3175
Size: 30 165 30 1475 30 1 30 236
Cuts at: 3175 3205 4223 4253 4442 4472 4490 4520 4593
Size: 30 1018 30 189 30 18 30 73
Cuts at: 4593 4623 4921 4951 1178
Size: 30 298 30 4082
Fragments arranged by size:
4082 1475 1018 298 236 189 165 73
30 30 30 30 30 30 30 30
30 30 18 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3886 3949 4001 4097 4228 1186
Size: 2700 63 52 96 131 4813
Fragments arranged by size:
4813 2700 131 96 63 52
BseYI C'CCAG_C
Cuts at: 3726 4168 7058 7554 3726
Size: 442 2890 496 4027
Fragments arranged by size:
4027 2890 496 442
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2098 2897 3536 3596 3598 3691 3920
Size: 573 362 799 639 60 2 93 229
Cuts at: 3920 4137 4247 4529 1163
Size: 217 110 282 4489
Fragments arranged by size:
4489 799 639 573 362 282 229 217
110 93 60 2
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4270 5088 5305 6066 7094 1540
Size: 275 186 2269 818 217 761 1028 2301
Fragments arranged by size:
2301 2269 1028 818 761 275 217 186
BsiHKAI G_wGCw'C
Cuts at: 5916 6001 7072 5916
Size: 85 1071 6699
Fragments arranged by size:
6699 1071 85
BsmI GAATG_Cn'
Cuts at: 2504 2545 4408 5366 5459 2504
Size: 41 1863 958 93 4900
Fragments arranged by size:
4900 1863 958 93 41
BsmBI CGTCTCn'nnnn_
Cuts at: 3195 3340 3375 3794 3195
Size: 145 35 419 7256
Fragments arranged by size:
7256 419 145 35
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2283 3396 4157 4168 5102 5174 5916
Size: 399 493 1113 761 11 934 72 742
Cuts at: 5916 6001 7072 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1113 1071 934 761 742 493 399
85 72 11
BspEI T'CCGG_A
Cuts at: 7518 7518
Size: 7855
BspHI T'CATG_A
Cuts at: 2656 5744 7474 2656
Size: 3088 1730 3037
Fragments arranged by size:
3088 3037 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4304 4968 5210 844
Size: 3460 664 242 3489
Fragments arranged by size:
3489 3460 664 242
BsrBI CCG'CTC
Cuts at: 1394 1823 3502 4345 5742 1394
Size: 429 1679 843 1397 3507
Fragments arranged by size:
3507 1679 1397 843 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3216 3694 3714 3752 6350 6524 65
Size: 1183 1968 478 20 38 2598 174 1396
Fragments arranged by size:
2598 1968 1396 1183 478 174 38 20
BsrFI r'CCGG_y
Cuts at: 1700 1810 3971 6499 6686 1700
Size: 110 2161 2528 187 2869
Fragments arranged by size:
2869 2528 2161 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7855
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7855
BssSI C'ACGA_G
Cuts at: 2799 3626 4472 4547 5915 6927 2799
Size: 827 846 75 1368 1012 3727
Fragments arranged by size:
3727 1368 1012 846 827 75
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7855
BstBI TT'CG_AA
Cuts at: 3069 4400 6659 3069
Size: 1331 2259 4265
Fragments arranged by size:
4265 2259 1331
BstEII G'GTnAC_C
Cuts at: 1184 3227 1184
Size: 2043 5812
BstXI CCAn_nnnn'nTGG
Cuts at: 2099 4838 2099
Size: 2739 5116
BstYI r'GATC_y
Cuts at: 1972 2395 2746 2976 3780 4045 4976 5185 5552
Size: 423 351 230 804 265 931 209 367
Cuts at: 5552 5937 5954 7406 7492 7504 7850 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 931 804 423 385 367 351
346 265 230 209 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2098 2694 3465 3487 3918 4597
Size: 552 939 94 596 771 22 431 679
Cuts at: 4597 513
Size: 3771
Fragments arranged by size:
3771 939 771 679 596 552 431 94
22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2455 3330 487
Size: 683 56 1229 875 5012
Fragments arranged by size:
5012 1229 875 683 56
BtsI GCAGTG_nn'
Cuts at: 2643 4484 5371 6165 6185 2643
Size: 1841 887 794 20 4313
Fragments arranged by size:
4313 1841 887 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7855
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7820
DraI TTT'AAA
Cuts at: 2680 5159 2680
Size: 2479 5376
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4337 6862 809
Size: 507 3021 2525 1802
Fragments arranged by size:
3021 2525 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3622 4267 4465
Size: 54 1425 50 275 18 1792 645 198
Cuts at: 4465 5009 5302 6191 7820 8
Size: 544 293 889 1629 43
Fragments arranged by size:
1792 1629 1425 889 645 544 293 275
198 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4267 5302 1537
Size: 275 2455 1035 4090
Fragments arranged by size:
4090 2455 1035 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2184 2313 2370 4172 5785 1353
Size: 831 129 57 1802 1613 3423
Fragments arranged by size:
3423 1802 1613 831 129 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4274 4289 6442 6816 6962 387
Size: 1064 138 2685 15 2153 374 146 1280
Fragments arranged by size:
2685 2153 1280 1064 374 146 138 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2546 2735 2936 3001 3361 3815 4112
Size: 393 914 189 201 65 360 454 297
Cuts at: 4112 4316 4667 5918 6515 7302 7570 1239
Size: 204 351 1251 597 787 268 1524
Fragments arranged by size:
1524 1251 914 787 597 454 393 360
351 297 268 204 201 189 65
EcoNI CCTnn'n_nnAGG
Cuts at: 3868 3868
Size: 7855
EcoO109I rG'GnC_Cy
Cuts at: 3892 4087 4423 4503 5098 3892
Size: 195 336 80 595 6649
Fragments arranged by size:
6649 595 336 195 80
EcoRI G'AATT_C
Cuts at: 4130 4660 5164 5542 4130
Size: 530 504 378 6443
Fragments arranged by size:
6443 530 504 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2239 4588 2239
Size: 2349 5506
FspI TGC'GCA
Cuts at: 6361 6361
Size: 7855
HaeII r_GCGC'y
Cuts at: 1678 3465 3916 4544 7002 1678
Size: 1787 451 628 2458 2531
Fragments arranged by size:
2531 2458 1787 628 451
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2503 2535 2927 2959 3175
Size: 32 135 32 931 32 392 32 216
Cuts at: 3175 3207 3650 3682 4223 4255 6496 6528 6570
Size: 32 443 32 541 32 2241 32 42
Cuts at: 6570 6602 1373
Size: 32 2626
Fragments arranged by size:
2626 2241 931 541 443 392 216 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5199 5445 669
Size: 988 281 3261 246 3079
Fragments arranged by size:
3261 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2852 748
Size: 2104 5751
HpaI GTT'AAC
Cuts at: 5445 5445
Size: 7855
KasI G'GCGC_C
Cuts at: 3461 3461
Size: 7855
KpnI G_GTAC'C
Cuts at: 4694 5180 4694
Size: 486 7369
MfeI C'AATT_G
Cuts at: 5454 5454
Size: 7855
MluI A'CGCG_T
Cuts at: 7731 7731
Size: 7855
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4050 6969 7153 1305
Size: 2745 2919 184 2007
Fragments arranged by size:
2919 2745 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4467 5011 10
Size: 54 1425 343 2635 544 2854
Fragments arranged by size:
2854 2635 1425 544 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 5813 6172 6331 518
Size: 648 4647 359 159 2042
Fragments arranged by size:
4647 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2824 3726 4246 4838 5271 5948 7096 7341
Size: 1218 902 520 592 433 677 1148 245
Cuts at: 7341 1606
Size: 2120
Fragments arranged by size:
2120 1218 1148 902 677 592 520 433
245
NaeI GCC'GGC
Cuts at: 3973 3973
Size: 7855
NarI GG'CG_CC
Cuts at: 3462 3462
Size: 7855
NcoI C'CATG_G
Cuts at: 513 1065 2004 2098 2694 3465 3487 513
Size: 552 939 94 596 771 22 4881
Fragments arranged by size:
4881 939 771 596 552 94 22
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7855
NgoMIV G'CCGG_C
Cuts at: 3971 3971
Size: 7855
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7855
NotI GC'GGCC_GC
Cuts at: 5302 5302
Size: 7855
NspI r_CATG'y
Cuts at: 1132 1229 4253 5213 6758 1132
Size: 97 3024 960 1545 2229
Fragments arranged by size:
3024 2229 1545 960 97
PasI CC'CwG_GG
Cuts at: 1348 5095 1348
Size: 3747 4108
PciI A'CATG_T
Cuts at: 6754 6754
Size: 7855
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2353 1108
Size: 1245 6610
PmeI GTTT'AAAC
Cuts at: 5159 5159
Size: 7855
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2353 2385 2459 2491 4593 4625 5924 5956 7437
Size: 32 74 32 2102 32 1299 32 1481
Cuts at: 7437 7469 2353
Size: 32 2739
Fragments arranged by size:
2739 2102 1481 1299 74 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 4087 4423 4087
Size: 336 7519
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7855
PsiI TTA'TAA
Cuts at: 5425 5425
Size: 7855
PspOMI G'GGCC_C
Cuts at: 5098 5098
Size: 7855
PstI C_TGCA'G
Cuts at: 830 3762 5207 830
Size: 2932 1445 3478
Fragments arranged by size:
3478 2932 1445
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7855
PvuII CAG'CTG
Cuts at: 2824 4838 2824
Size: 2014 5841
RsrII CG'GwC_CG
Cuts at: 5151 5151
Size: 7855
SalI G'TCGA_C
Cuts at: 1936 5197 1936
Size: 3261 4594
SapI GCTCTTCn'nnn_
Cuts at: 2184 2370 2184
Size: 186 7669
SbfI CC_TGCA'GG
Cuts at: 5207 5207
Size: 7855
ScaI AGT'ACT
Cuts at: 6103 7815 6103
Size: 1712 6143
SfcI C'TryA_G
Cuts at: 826 1045 3758 4003 4493 4641 5203 6338 7019
Size: 219 2713 245 490 148 562 1135 681
Cuts at: 7019 7210 826
Size: 191 1471
Fragments arranged by size:
2713 1471 1135 681 562 490 245 219
191 148
SfiI GGCCn_nnn'nGGCC
Cuts at: 5017 5017
Size: 7855
SfoI GGC'GCC
Cuts at: 3463 3463
Size: 7855
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7855
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7855
SmaI CCC'GGG
Cuts at: 1872 5182 1872
Size: 3310 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3152 3809 3976 5959 6860 7122
Size: 197 926 1209 657 167 1983 901 262
Cuts at: 7122 820
Size: 1553
Fragments arranged by size:
1983 1553 1209 926 901 657 262 197
167
SnaBI TAC'GTA
Cuts at: 493 5646 493
Size: 5153 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7855
SphI G_CATG'C
Cuts at: 1132 5213 1132
Size: 4081 3774
SspI AAT'ATT
Cuts at: 5 52 5779 5
Size: 47 5727 2081
Fragments arranged by size:
5727 2081 47
StuI AGG'CCT
Cuts at: 1517 5020 1517
Size: 3503 4352
StyI C'CwwG_G
Cuts at: 513 1065 2004 2098 2694 3456 3465 3487 3736
Size: 552 939 94 596 762 9 22 249
Cuts at: 3736 5294 513
Size: 1558 3074
Fragments arranged by size:
3074 1558 939 762 596 552 249 94
22 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5882 5899 6052 6237 6682 325
Size: 1255 4302 17 153 185 445 1498
Fragments arranged by size:
4302 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6101 7813 96
Size: 274 80 33 51 1443 4124 1712 138
Fragments arranged by size:
4124 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2909 2941 4490 4522 4921 4953 7815 7847 2909
Size: 32 1549 32 399 32 2862 32 2917
Fragments arranged by size:
2917 2862 1549 399 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3187 4109 1316
Size: 1871 922 5062
Fragments arranged by size:
5062 1871 922
XbaI T'CTAG_A
Cuts at: 4048 5191 4048
Size: 1143 6712
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2812 3463 2812
Size: 651 7204
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7855
XmaI C'CCGG_G
Cuts at: 1870 5180 1870
Size: 3310 4545
XmnI GAAnn'nnTTC
Cuts at: 3087 4680 4858 5984 3087
Size: 1593 178 1126 4958
Fragments arranged by size:
4958 1593 1126 178
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5663 276
Size: 53 83 186 936 4129 2468
Fragments arranged by size:
4129 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII RsrII SalI SapI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I
Bsu36I DraIII EcoICRI EcoRV FseI FspAI NruI NsiI
PacI PfoI PmlI PspXI PsrI SacI SacII SanDI
SexAI SrfI SwaI