Restriction map for
pFN21AF0021
(Circular) (Six-base) MAPSORT of: pFN21AF0021.seq Check: 3489 from: 1 to: 6193
pFN21AF0021 6193 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2512 2512
Size: 6193
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2685 3045 4003 278
Size: 53 83 186 936 1149 360 958 2468
Fragments arranged by size:
2468 1149 958 936 360 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2847 3536 1937
Size: 910 689 4594
Fragments arranged by size:
4594 910 689
Acc65I G'GTAC_C
Cuts at: 3514 3514
Size: 6193
AclI AA'CG_TT
Cuts at: 1563 4320 4693 1563
Size: 2757 373 3063
Fragments arranged by size:
3063 2757 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4256 5640 5908 1632
Size: 2624 1384 268 1917
Fragments arranged by size:
2624 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5996
AflIII A'CryG_T
Cuts at: 1203 2064 3012 5092 6069 1203
Size: 861 948 2080 977 1327
Fragments arranged by size:
2080 1327 977 948 861
AgeI A'CCGG_T
Cuts at: 2592 5024 2592
Size: 2432 3761
AhdI GACnn_n'nnGTC
Cuts at: 3259 4922 3259
Size: 1663 4530
AleI CACnn'nnGTG
Cuts at: 1166 2189 3218 1166
Size: 1023 1029 4141
Fragments arranged by size:
4141 1029 1023
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6193
AlwNI CAG_nnn'CTG
Cuts at: 2710 3936 5508 2710
Size: 1226 1572 3395
Fragments arranged by size:
3395 1572 1226
ApaI G_GGCC'C
Cuts at: 2607 2607
Size: 6193
ApaLI G'TGCA_C
Cuts at: 4250 5406 4250
Size: 1156 5037
ApoI r'AATT_y
Cuts at: 1493 3502 3728 3880 5989 1493
Size: 2009 226 152 2109 1697
Fragments arranged by size:
2109 2009 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4747 160
Size: 872 3715 1606
Fragments arranged by size:
3715 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6193
AvaI C'yCGr_G
Cuts at: 1870 1943 2815 3518 1870
Size: 73 872 703 4545
Fragments arranged by size:
4545 872 703 73
BamHI G'GATC_C
Cuts at: 3523 3890 3523
Size: 367 5826
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2793 3220 3293 3514 4969
Size: 325 217 627 1006 427 73 221 1455
Cuts at: 4969 618
Size: 1842
Fragments arranged by size:
1842 1455 1006 627 427 325 221 217
73
BanII G_rGCy'C
Cuts at: 1391 2607 3512 1391
Size: 1216 905 4072
Fragments arranged by size:
4072 1216 905
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2696 3082 3152 928
Size: 971 797 386 70 3969
Fragments arranged by size:
3969 971 797 386 70
BbvCI CC'TCA_GC
Cuts at: 3476 3476
Size: 6193
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4384 4418 1903
Size: 34 2447 34 3678
Fragments arranged by size:
3678 2447 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4085 5295 4085
Size: 1210 4983
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6193
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4804 136
Size: 107 122 71 631 605 3132 1525
Fragments arranged by size:
3132 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6188 6188
Size: 6193
BlpI GC'TnA_GC
Cuts at: 3610 3610
Size: 6193
Bme1580I G_kGCm'C
Cuts at: 1790 2087 2508 2607 2778 2796 3296 4254 5410
Size: 297 421 99 171 18 500 958 1156
Cuts at: 5410 1790
Size: 2573
Fragments arranged by size:
2573 1156 958 500 421 297 171 99
18
BmgBI CAC'GTC
Cuts at: 1318 2357 1318
Size: 1039 5154
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3924 4882 442
Size: 416 53 83 399 24 2507 958 1753
Fragments arranged by size:
2507 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6193
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3278 4853 1239
Size: 2039 1575 2579
Fragments arranged by size:
2579 2039 1575
Bpu10I CC'TnA_GC
Cuts at: 3476 3476
Size: 6193
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2234 4318 5183 5481 2234
Size: 2084 865 298 2946
Fragments arranged by size:
2946 2084 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2018 2472 3476 4856 882
Size: 617 519 454 1004 1380 2219
Fragments arranged by size:
2219 1380 1004 617 519 454
BsaAI yAC'GTr
Cuts at: 493 1204 2938 3174 3984 493
Size: 711 1734 236 810 2702
Fragments arranged by size:
2702 1734 810 711 236
BsaBI GATnn'nnATC
Cuts at: 2768 6187 2768
Size: 3419 2774
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2622 2682 3042 4000
Size: 53 83 186 936 1089 60 360 958
Cuts at: 4000 4382 5017 275
Size: 382 635 1451
Fragments arranged by size:
1451 1089 958 936 635 382 360 186
83 60 53
BsaWI w'CCGG_w
Cuts at: 2592 2823 4626 5024 5298 5445 5856 2592
Size: 231 1803 398 274 147 411 2929
Fragments arranged by size:
2929 1803 411 398 274 231 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2258 2288 1178
Size: 30 165 30 855 30 5083
Fragments arranged by size:
5083 855 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2263 2270 2645 2648 2887 2954 1186
Size: 1077 7 375 3 239 67 4425
Fragments arranged by size:
4425 1077 375 239 67 7 3
BseYI C'CCAG_C
Cuts at: 2598 3351 5396 5892 2598
Size: 753 2045 496 2899
Fragments arranged by size:
2899 2045 753 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2369 3229 1163
Size: 573 633 860 4127
Fragments arranged by size:
4127 860 633 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3643 4404 5432 1540
Size: 275 186 1642 761 1028 2301
Fragments arranged by size:
2301 1642 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4254 4339 5410 4254
Size: 85 1071 5037
Fragments arranged by size:
5037 1071 85
BsmI GAATG_Cn'
Cuts at: 2111 3704 3797 2111
Size: 1593 93 4507
Fragments arranged by size:
4507 1593 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2347 2899 2347
Size: 552 5641
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2087 2508 2607 2778 2796 3296 3512
Size: 399 297 421 99 171 18 500 216
Cuts at: 3512 4254 4339 5410 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 500 421 399 297 216
171 99 85 18
BspEI T'CCGG_A
Cuts at: 2823 5856 2823
Size: 3033 3160
BspHI T'CATG_A
Cuts at: 2166 2745 4082 5812 2166
Size: 579 1337 1730 2547
Fragments arranged by size:
2547 1730 1337 579
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2512 2537 2709 3548 844
Size: 1668 25 172 839 3489
Fragments arranged by size:
3489 1668 839 172 25
BsrBI CCG'CTC
Cuts at: 1394 1823 2320 4080 1394
Size: 429 497 1760 3507
Fragments arranged by size:
3507 1760 497 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2551 3117 4688 4862 65
Size: 1183 1303 566 1571 174 1396
Fragments arranged by size:
1571 1396 1303 1183 566 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2592 3296 4837 5024 1700
Size: 110 782 704 1541 187 2869
Fragments arranged by size:
2869 1541 782 704 187 110
BsrGI T'GTAC_A
Cuts at: 96 2061 3015 96
Size: 1965 954 3274
Fragments arranged by size:
3274 1965 954
BssHII G'CGCG_C
Cuts at: 1925 3032 1925
Size: 1107 5086
BssSI C'ACGA_G
Cuts at: 4253 5265 4253
Size: 1012 5181
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2872 794
Size: 2078 4115
BstBI TT'CG_AA
Cuts at: 4997 4997
Size: 6193
BstEII G'GTnAC_C
Cuts at: 1184 2524 1184
Size: 1340 4853
BstXI CCAn_nnnn'nTGG
Cuts at: 3217 3217
Size: 6193
BstYI r'GATC_y
Cuts at: 1972 2360 2706 3523 3890 4275 4292 5744 5830
Size: 388 346 817 367 385 17 1452 86
Cuts at: 5830 5842 6188 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 817 388 385 367 346 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2202 2992 3179 513
Size: 552 939 198 790 187 3527
Fragments arranged by size:
3527 939 790 552 198 187
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2307 487
Size: 683 56 1081 4373
Fragments arranged by size:
4373 1081 683 56
BtsI GCAGTG_nn'
Cuts at: 2118 3334 3709 4503 4523 2118
Size: 1216 375 794 20 3788
Fragments arranged by size:
3788 1216 794 375 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6193
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2170 2205 529
Size: 35 1606 35 4517
Fragments arranged by size:
4517 1606 35 35
DraI TTT'AAA
Cuts at: 3497 3497
Size: 6193
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5200 809
Size: 507 3884 1802
Fragments arranged by size:
3884 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3054 3462 3640
Size: 54 1425 50 275 18 1224 408 178
Cuts at: 3640 4529 6158 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1224 889 408 275 178 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3640 1537
Size: 275 1828 4090
Fragments arranged by size:
4090 1828 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2047 2228 3086 3158 4123 1353
Size: 694 181 858 72 965 3423
Fragments arranged by size:
3423 965 858 694 181 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3389 4780 5154 5300 387
Size: 1064 138 1800 1391 374 146 1280
Fragments arranged by size:
1800 1391 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3278 4256 4853 5640 5908 1239
Size: 393 1646 978 597 787 268 1524
Fragments arranged by size:
1646 1524 978 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2252 2519 2252
Size: 267 5926
EcoO109I rG'GnC_Cy
Cuts at: 2603 2604 3282 3390 2603
Size: 1 678 108 5406
Fragments arranged by size:
5406 678 108 1
EcoRI G'AATT_C
Cuts at: 3502 3880 3502
Size: 378 5815
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3169 3169
Size: 6193
FspI TGC'GCA
Cuts at: 3134 4699 3134
Size: 1565 4628
HaeII r_GCGC'y
Cuts at: 1678 2538 2908 5340 1678
Size: 860 370 2432 2531
Fragments arranged by size:
2531 2432 860 370
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2255 2258 2287 2290 2635
Size: 32 135 32 683 3 29 3 345
Cuts at: 2635 2667 3245 3277 4834 4866 4908 4940 1373
Size: 32 578 32 1557 32 42 32 2626
Fragments arranged by size:
2626 1557 683 578 345 135 42 32
32 32 32 32 32 29 3 3
HincII GTy'rAC
Cuts at: 669 1657 1938 2440 3537 3783 669
Size: 988 281 502 1097 246 3079
Fragments arranged by size:
3079 1097 988 502 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6193
HpaI GTT'AAC
Cuts at: 3783 3783
Size: 6193
KpnI G_GTAC'C
Cuts at: 3518 3518
Size: 6193
MfeI C'AATT_G
Cuts at: 3792 3792
Size: 6193
MluI A'CGCG_T
Cuts at: 6069 6069
Size: 6193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5307 5491 1305
Size: 4002 184 2007
Fragments arranged by size:
4002 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4371
Fragments arranged by size:
4371 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2189 2522 2750 3011 3122 3218 4151
Size: 648 1023 333 228 261 111 96 933
Cuts at: 4151 4510 4669 518
Size: 359 159 2042
Fragments arranged by size:
2042 1023 933 648 359 333 261 228
159 111 96
MspA1I CmG'CkG
Cuts at: 1606 2994 3059 3355 3609 4286 5434 5679 1606
Size: 1388 65 296 254 677 1148 245 2120
Fragments arranged by size:
2120 1388 1148 677 296 254 245 65
NcoI C'CATG_G
Cuts at: 513 1065 2004 2202 513
Size: 552 939 198 4504
Fragments arranged by size:
4504 939 552 198
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6193
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6193
NotI GC'GGCC_GC
Cuts at: 3640 3640
Size: 6193
NspI r_CATG'y
Cuts at: 1132 1229 2068 3016 3551 5096 1132
Size: 97 839 948 535 1545 2229
Fragments arranged by size:
2229 1545 948 839 535 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6193
PciI A'CATG_T
Cuts at: 2064 3012 5092 2064
Size: 948 2080 3165
Fragments arranged by size:
3165 2080 948
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3256 1108
Size: 2148 4045
PfoI T'CCnGG_A
Cuts at: 2304 2928 2304
Size: 624 5569
PmeI GTTT'AAAC
Cuts at: 3497 3497
Size: 6193
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4262 4294 5775 5807 4262
Size: 32 1481 32 4648
Fragments arranged by size:
4648 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3390 3390
Size: 6193
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6193
PsiI TTA'TAA
Cuts at: 3763 3763
Size: 6193
PspOMI G'GGCC_C
Cuts at: 2603 2603
Size: 6193
PstI C_TGCA'G
Cuts at: 830 2212 2534 2706 3545 830
Size: 1382 322 172 839 3478
Fragments arranged by size:
3478 1382 839 322 172
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6193
PvuII CAG'CTG
Cuts at: 3355 3355
Size: 6193
RsrII CG'GwC_CG
Cuts at: 2663 2663
Size: 6193
SacII CC_GC'GG
Cuts at: 2995 2995
Size: 6193
SalI G'TCGA_C
Cuts at: 1936 3535 1936
Size: 1599 4594
SbfI CC_TGCA'GG
Cuts at: 3545 3545
Size: 6193
ScaI AGT'ACT
Cuts at: 4441 6153 4441
Size: 1712 4481
SfcI C'TryA_G
Cuts at: 826 1045 2208 2530 2702 3541 4676 5357 5548
Size: 219 1163 322 172 839 1135 681 191
Cuts at: 5548 826
Size: 1471
Fragments arranged by size:
1471 1163 1135 839 681 322 219 191
172
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6193
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6193
SmaI CCC'GGG
Cuts at: 1872 3520 1872
Size: 1648 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2249 4297 5198 5460 820
Size: 197 926 306 2048 901 262 1553
Fragments arranged by size:
2048 1553 926 901 306 262 197
SnaBI TAC'GTA
Cuts at: 493 3984 493
Size: 3491 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6193
SphI G_CATG'C
Cuts at: 1132 3551 1132
Size: 2419 3774
SspI AAT'ATT
Cuts at: 5 52 4117 5
Size: 47 4065 2081
Fragments arranged by size:
4065 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6193
StyI C'CwwG_G
Cuts at: 513 1065 2004 2106 2202 3632 513
Size: 552 939 102 96 1430 3074
Fragments arranged by size:
3074 1430 939 552 102 96
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3172 3263 3365 3403 4220 4237 4390
Size: 1255 1592 91 102 38 817 17 153
Cuts at: 4390 4575 5020 325
Size: 185 445 1498
Fragments arranged by size:
1592 1498 1255 817 445 185 153 102
91 38 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2061 3015 4439
Size: 274 80 33 51 1443 84 954 1424
Cuts at: 4439 6151 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1424 954 274 138 84 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6153 6185 6153
Size: 32 6161
Tth111I GACn'n_nGTC
Cuts at: 1316 3194 1316
Size: 1878 4315
XbaI T'CTAG_A
Cuts at: 3529 3529
Size: 6193
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3214 3359 3214
Size: 145 6048
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6193
XmaI C'CCGG_G
Cuts at: 1870 3518 1870
Size: 1648 4545
XmnI GAAnn'nnTTC
Cuts at: 4322 4322
Size: 6193
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2683 3043 4001 276
Size: 53 83 186 936 1149 360 958 2468
Fragments arranged by size:
2468 1149 958 936 360 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
RsrII SacII SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PspXI PsrI SacI SanDI SapI SexAI SfiI SfoI
SrfI SwaI