Restriction map for
pFN21AF0006
(Circular) (Six-base) MAPSORT of: pFN21AF0006.seq Check: 5952 from: 1 to: 6952
pFN21AF0006 6952 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2032 3988 2032
Size: 1956 4996
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4762 278
Size: 53 83 186 936 3226 2468
Fragments arranged by size:
3226 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4295 1937
Size: 2358 4594
Acc65I G'GTAC_C
Cuts at: 4056 4273 4056
Size: 217 6735
AclI AA'CG_TT
Cuts at: 1563 5079 5452 1563
Size: 3516 373 3063
Fragments arranged by size:
3516 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2698 3752 3838 4120 4211 4216 5015 6399
Size: 1066 1054 86 282 91 5 799 1384
Cuts at: 6399 6667 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1066 1054 799 282 268 91
86 5
AflII C'TTAA_G
Cuts at: 820 1017 3707 820
Size: 197 2690 4065
Fragments arranged by size:
4065 2690 197
AflIII A'CryG_T
Cuts at: 1203 5851 6828 1203
Size: 4648 977 1327
Fragments arranged by size:
4648 1327 977
AgeI A'CCGG_T
Cuts at: 5783 5783
Size: 6952
AhdI GACnn_n'nnGTC
Cuts at: 5681 5681
Size: 6952
AleI CACnn'nnGTG
Cuts at: 1166 2371 3092 3530 1166
Size: 1205 721 438 4588
Fragments arranged by size:
4588 1205 721 438
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2561 2741 1184
Size: 1377 180 5395
Fragments arranged by size:
5395 1377 180
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2981 3013 2981
Size: 32 6920
AlwNI CAG_nnn'CTG
Cuts at: 2227 2779 3157 4003 4189 4695 6267 2227
Size: 552 378 846 186 506 1572 2912
Fragments arranged by size:
2912 1572 846 552 506 378 186
ApaI G_GGCC'C
Cuts at: 2395 2575 2395
Size: 180 6772
ApaLI G'TGCA_C
Cuts at: 3346 5009 6165 3346
Size: 1663 1156 4133
Fragments arranged by size:
4133 1663 1156
ApoI r'AATT_y
Cuts at: 1493 4261 4487 4639 6748 1493
Size: 2768 226 152 2109 1697
Fragments arranged by size:
2768 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5506 160
Size: 872 4474 1606
Fragments arranged by size:
4474 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6952
AvaI C'yCGr_G
Cuts at: 1870 1943 2922 4084 4277 1870
Size: 73 979 1162 193 4545
Fragments arranged by size:
4545 1162 979 193 73
BamHI G'GATC_C
Cuts at: 4282 4649 4282
Size: 367 6585
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2545 2612 3167 3450 3755
Size: 325 217 627 758 67 555 283 305
Cuts at: 3755 4056 4273 5728 618
Size: 301 217 1455 1842
Fragments arranged by size:
1842 1455 758 627 555 325 305 301
283 217 217 67
BanII G_rGCy'C
Cuts at: 1391 2395 2575 2921 3570 3990 4113 4161 4271
Size: 1004 180 346 649 420 123 48 110
Cuts at: 4271 1391
Size: 4072
Fragments arranged by size:
4072 1004 649 420 346 180 123 110
48
BbeI G_GCGC'C
Cuts at: 3454 3759 3454
Size: 305 6647
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5981
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3770 3804 4104 4138 5143 5177 1903
Size: 34 1833 34 300 34 1005 34 3678
Fragments arranged by size:
3678 1833 1005 300 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4844 6054 4844
Size: 1210 5742
BclI T'GATC_A
Cuts at: 1548 2829 1548
Size: 1281 5671
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3582 3710 5563
Size: 107 122 71 631 605 1910 128 1853
Cuts at: 5563 136
Size: 1525
Fragments arranged by size:
1910 1853 1525 631 605 128 122 107
71
BglII A'GATC_T
Cuts at: 2073 6947 2073
Size: 4874 2078
BlpI GC'TnA_GC
Cuts at: 4369 4369
Size: 6952
Bme1580I G_kGCm'C
Cuts at: 1790 2395 2550 2575 2615 3350 5013 6169 1790
Size: 605 155 25 40 735 1663 1156 2573
Fragments arranged by size:
2573 1663 1156 735 605 155 40 25
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6952
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4162 4683 5641
Size: 416 53 83 399 24 2745 521 958
Cuts at: 5641 442
Size: 1753
Fragments arranged by size:
2745 1753 958 521 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6952
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2194 3710 2194
Size: 1516 5436
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2164 2267 2669 2786 3278 3366 4015 4037
Size: 925 103 402 117 492 88 649 22
Cuts at: 4037 5612 1239
Size: 1575 2579
Fragments arranged by size:
2579 1575 925 649 492 402 117 103
88 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2945 3251 5077 5942 6240 2945
Size: 306 1826 865 298 3657
Fragments arranged by size:
3657 1826 865 306 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2453 5615 882
Size: 617 954 3162 2219
Fragments arranged by size:
3162 2219 954 617
BsaAI yAC'GTr
Cuts at: 493 1204 3427 4743 493
Size: 711 2223 1316 2702
Fragments arranged by size:
2702 2223 1316 711
BsaBI GATnn'nnATC
Cuts at: 6946 6946
Size: 6952
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2661 3451 3756 4200
Size: 53 83 186 936 1128 790 305 444
Cuts at: 4200 4759 5141 5776 275
Size: 559 382 635 1451
Fragments arranged by size:
1451 1128 936 790 635 559 444 382
305 186 83 53
BsaWI w'CCGG_w
Cuts at: 5385 5783 6057 6204 6615 5385
Size: 398 274 147 411 5722
Fragments arranged by size:
5722 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2903 2933 2981 3011 3803
Size: 30 165 30 1500 30 48 30 792
Cuts at: 3803 3833 4040 4070 1178
Size: 30 207 30 4060
Fragments arranged by size:
4060 1500 792 207 165 48 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2666 2669 3797 3905 1186
Size: 1480 3 1128 108 4233
Fragments arranged by size:
4233 1480 1128 108 3
BseYI C'CCAG_C
Cuts at: 2507 2728 2944 3207 3499 3555 4089 4236 6155
Size: 221 216 263 292 56 534 147 1919
Cuts at: 6155 6651 2507
Size: 496 2808
Fragments arranged by size:
2808 1919 534 496 292 263 221 216
147 56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2822 2939 3932 3989 1163
Size: 573 1086 117 993 57 4126
Fragments arranged by size:
4126 1086 993 573 117 57
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4402 5163 6191 1540
Size: 275 186 2401 761 1028 2301
Fragments arranged by size:
2401 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2568 3350 3437 3552 3570 3843 4026 4113 4161
Size: 782 87 115 18 273 183 87 48
Cuts at: 4161 5013 5098 6169 2568
Size: 852 85 1071 3351
Fragments arranged by size:
3351 1071 852 782 273 183 115 87
87 85 48 18
BsmI GAATG_Cn'
Cuts at: 4463 4556 4463
Size: 93 6859
BsmBI CGTCTCn'nnnn_
Cuts at: 2305 2305
Size: 6952
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2395 2550 2568 2575 2615 2921 3350
Size: 399 605 155 18 7 40 306 429
Cuts at: 3350 3437 3552 3570 3843 3990 4026 4113 4161
Size: 87 115 18 273 147 36 87 48
Cuts at: 4161 4271 5013 5098 6169 1391
Size: 110 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 605 429 399 306 273
155 147 115 110 87 87 85 48
40 36 18 18 7
BspEI T'CCGG_A
Cuts at: 6615 6615
Size: 6952
BspHI T'CATG_A
Cuts at: 4841 6571 4841
Size: 1730 5222
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2032 2499 2703 2787 3988 4307 844
Size: 1188 467 204 84 1201 319 3489
Fragments arranged by size:
3489 1201 1188 467 319 204 84
BsrBI CCG'CTC
Cuts at: 1394 1823 2313 2328 3481 3969 4839 1394
Size: 429 490 15 1153 488 870 3507
Fragments arranged by size:
3507 1153 870 490 488 429 15
BsrDI GCAATG_nn'
Cuts at: 65 1248 3135 5447 5621 65
Size: 1183 1887 2312 174 1396
Fragments arranged by size:
2312 1887 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2342 2615 3077 3164 3447 5596 5783
Size: 110 532 273 462 87 283 2149 187
Cuts at: 5783 1700
Size: 2869
Fragments arranged by size:
2869 2149 532 462 283 273 187 110
87
BsrGI T'GTAC_A
Cuts at: 96 2151 96
Size: 2055 4897
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6952
BssSI C'ACGA_G
Cuts at: 3416 3680 5012 6024 3416
Size: 264 1332 1012 4344
Fragments arranged by size:
4344 1332 1012 264
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2517 3944 4003 794
Size: 1723 1427 59 3743
Fragments arranged by size:
3743 1723 1427 59
BstBI TT'CG_AA
Cuts at: 3720 5756 3720
Size: 2036 4916
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6952
BstXI CCAn_nnnn'nTGG
Cuts at: 2188 2373 2728 2869 3073 3604 2188
Size: 185 355 141 204 531 5536
Fragments arranged by size:
5536 531 355 204 185 141
BstYI r'GATC_y
Cuts at: 1972 2073 2275 4282 4649 5034 5051 6503 6589
Size: 101 202 2007 367 385 17 1452 86
Cuts at: 6589 6601 6947 1972
Size: 12 346 1977
Fragments arranged by size:
2007 1977 1452 385 367 346 202 101
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 3099 4044 513
Size: 552 2034 945 3421
Fragments arranged by size:
3421 2034 945 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2449 3625 487
Size: 683 56 1223 1176 3814
Fragments arranged by size:
3814 1223 1176 683 56
BtsI GCAGTG_nn'
Cuts at: 2834 3228 3550 3945 4468 5262 5282 2834
Size: 394 322 395 523 794 20 4504
Fragments arranged by size:
4504 794 523 395 394 322 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6952
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6917
DraI TTT'AAA
Cuts at: 4256 4256
Size: 6952
DraIII CAC_nnn'GTG
Cuts at: 2269 2269
Size: 6952
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2750 5959 809
Size: 507 1434 3209 1802
Fragments arranged by size:
3209 1802 1434 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2079 2340 2625
Size: 54 1425 50 275 18 249 261 285
Cuts at: 2625 3063 3075 3096 4399 5288 6917 8
Size: 438 12 21 1303 889 1629 43
Fragments arranged by size:
1629 1425 1303 889 438 285 275 261
249 54 50 43 21 18 12
EagI C'GGCC_G
Cuts at: 1537 1812 4399 1537
Size: 275 2587 4090
Fragments arranged by size:
4090 2587 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2094 2399 2964 3245 4163 4235 4882 1353
Size: 741 305 565 281 918 72 647 3423
Fragments arranged by size:
3423 918 741 647 565 305 281 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5539 5913 6059 387
Size: 1064 138 3950 374 146 1280
Fragments arranged by size:
3950 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3568 4111 4159 3568
Size: 543 48 6361
Fragments arranged by size:
6361 543 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2164 2267 2669 2698 2786 3278 3366
Size: 393 532 103 402 29 88 492 88
Cuts at: 3366 3752 3838 4015 4037 4120 4211 4216 5015
Size: 386 86 177 22 83 91 5 799
Cuts at: 5015 5612 6399 6667 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 799 787 597 532 492 402 393
386 268 177 103 91 88 88 86
83 29 22 5
EcoNI CCTnn'n_nnAGG
Cuts at: 2651 2963 3185 4145 4193 2651
Size: 312 222 960 48 5410
Fragments arranged by size:
5410 960 312 222 48
EcoO109I rG'GnC_Cy
Cuts at: 2391 2392 2488 2572 2692 2752 2899 3204 3463
Size: 1 96 84 120 60 147 305 259
Cuts at: 3463 3690 3712 2391
Size: 227 22 5631
Fragments arranged by size:
5631 305 259 227 147 120 96 84
60 22 1
EcoRI G'AATT_C
Cuts at: 4261 4639 4261
Size: 378 6574
EcoRV GAT'ATC
Cuts at: 3022 3022
Size: 6952
FspI TGC'GCA
Cuts at: 2558 2891 4079 5458 2558
Size: 333 1188 1379 4052
Fragments arranged by size:
4052 1379 1188 333
HaeII r_GCGC'y
Cuts at: 1678 3115 3454 3759 6099 1678
Size: 1437 339 305 2340 2531
Fragments arranged by size:
2531 2340 1437 339 305
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3011 3043 3803 3835 5593
Size: 32 135 32 1439 32 760 32 1758
Cuts at: 5593 5625 5667 5699 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1758 1439 760 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4296 4542 669
Size: 988 281 2358 246 3079
Fragments arranged by size:
3079 2358 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6952
HpaI GTT'AAC
Cuts at: 4542 4542
Size: 6952
KasI G'GCGC_C
Cuts at: 3450 3755 3450
Size: 305 6647
KpnI G_GTAC'C
Cuts at: 4060 4277 4060
Size: 217 6735
MfeI C'AATT_G
Cuts at: 4551 4551
Size: 6952
MluI A'CGCG_T
Cuts at: 6828 6828
Size: 6952
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2475 3840 6066 6250 1305
Size: 1170 1365 2226 184 2007
Fragments arranged by size:
2226 2007 1365 1170 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2081 3065 10
Size: 54 1425 343 249 984 3897
Fragments arranged by size:
3897 1425 984 343 249 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2371 2543 2618 3092 3530 4214 4910
Size: 648 1205 172 75 474 438 684 696
Cuts at: 4910 5269 5428 518
Size: 359 159 2042
Fragments arranged by size:
2042 1205 696 684 648 474 438 359
172 159 75
MspA1I CmG'CkG
Cuts at: 1606 2371 2749 3211 3409 4236 4368 5045 6193
Size: 765 378 462 198 827 132 677 1148
Cuts at: 6193 6438 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 827 765 677 462 378 245
198 132
NaeI GCC'GGC
Cuts at: 2344 3079 3166 2344
Size: 735 87 6130
Fragments arranged by size:
6130 735 87
NarI GG'CG_CC
Cuts at: 3451 3756 3451
Size: 305 6647
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6400
NdeI CA'TA_TG
Cuts at: 387 2060 387
Size: 1673 5279
NgoMIV G'CCGG_C
Cuts at: 2342 3077 3164 2342
Size: 735 87 6130
Fragments arranged by size:
6130 735 87
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6952
NotI GC'GGCC_GC
Cuts at: 4399 4399
Size: 6952
NspI r_CATG'y
Cuts at: 1132 1229 2953 3947 4213 4310 5855 1132
Size: 97 1724 994 266 97 1545 2229
Fragments arranged by size:
2229 1724 1545 994 266 97 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6952
PciI A'CATG_T
Cuts at: 5851 5851
Size: 6952
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2269 3157 1108
Size: 1161 888 4903
Fragments arranged by size:
4903 1161 888
PfoI T'CCnGG_A
Cuts at: 2646 2802 3309 3612 4203 2646
Size: 156 507 303 591 5395
Fragments arranged by size:
5395 591 507 303 156
PmeI GTTT'AAAC
Cuts at: 4256 4256
Size: 6952
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2981 3013 5021 5053 6534 6566 2981
Size: 32 2008 32 1481 32 3367
Fragments arranged by size:
3367 2008 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2488 2692 2752 2899 3204 3690 2488
Size: 204 60 147 305 486 5750
Fragments arranged by size:
5750 486 305 204 147 60
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6952
PsiI TTA'TAA
Cuts at: 4522 4522
Size: 6952
PspOMI G'GGCC_C
Cuts at: 2391 2571 2391
Size: 180 6772
PstI C_TGCA'G
Cuts at: 830 2400 3368 3793 4304 830
Size: 1570 968 425 511 3478
Fragments arranged by size:
3478 1570 968 511 425
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6952
PvuII CAG'CTG
Cuts at: 2371 4236 2371
Size: 1865 5087
SacI G_AGCT'C
Cuts at: 3570 4113 4161 3570
Size: 543 48 6361
Fragments arranged by size:
6361 543 48
SalI G'TCGA_C
Cuts at: 1936 4294 1936
Size: 2358 4594
SanDI GG'GwC_CC
Cuts at: 3690 3690
Size: 6952
SapI GCTCTTCn'nnn_
Cuts at: 2964 3245 2964
Size: 281 6671
SbfI CC_TGCA'GG
Cuts at: 4304 4304
Size: 6952
ScaI AGT'ACT
Cuts at: 3635 5200 6912 3635
Size: 1565 1712 3675
Fragments arranged by size:
3675 1712 1565
SfcI C'TryA_G
Cuts at: 826 1045 2396 3364 3388 3789 4300 5435 6116
Size: 219 1351 968 24 401 511 1135 681
Cuts at: 6116 6307 826
Size: 191 1471
Fragments arranged by size:
1471 1351 1135 968 681 511 401 219
191 24
SfiI GGCCn_nnn'nGGCC
Cuts at: 3710 3710
Size: 6952
SfoI GGC'GCC
Cuts at: 3452 3757 3452
Size: 305 6647
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6952
SgrAI Cr'CCGG_yG
Cuts at: 1700 3447 1700
Size: 1747 5205
SmaI CCC'GGG
Cuts at: 1872 2924 4086 4279 1872
Size: 1052 1162 193 4545
Fragments arranged by size:
4545 1162 1052 193
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2960 3266 3707 5056 5957 6219
Size: 197 926 1017 306 441 1349 901 262
Cuts at: 6219 820
Size: 1553
Fragments arranged by size:
1553 1349 1017 926 901 441 306 262
197
SnaBI TAC'GTA
Cuts at: 493 4743 493
Size: 4250 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6952
SphI G_CATG'C
Cuts at: 1132 2953 3947 4310 1132
Size: 1821 994 363 3774
Fragments arranged by size:
3774 1821 994 363
SrfI GCCC'GGGC
Cuts at: 4086 4086
Size: 6952
SspI AAT'ATT
Cuts at: 5 52 4876 5
Size: 47 4824 2081
Fragments arranged by size:
4824 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6952
StyI C'CwwG_G
Cuts at: 513 1065 3459 4391 513
Size: 552 2394 932 3074
Fragments arranged by size:
3074 2394 932 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2270 2720 3547 4979 4996 5149 5334
Size: 1255 690 450 827 1432 17 153 185
Cuts at: 5334 5779 325
Size: 445 1498
Fragments arranged by size:
1498 1432 1255 827 690 450 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2085 2151 3633
Size: 274 80 33 51 1443 108 66 1482
Cuts at: 3633 5198 6910 96
Size: 1565 1712 138
Fragments arranged by size:
1712 1565 1482 1443 274 138 108 80
66 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3324 3356 6912 6944 3324
Size: 32 3556 32 3332
Fragments arranged by size:
3556 3332 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6952
XbaI T'CTAG_A
Cuts at: 4288 4288
Size: 6952
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3325 3466 3325
Size: 141 6811
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6952
XmaI C'CCGG_G
Cuts at: 1870 2922 4084 4277 1870
Size: 1052 1162 193 4545
Fragments arranged by size:
4545 1162 1052 193
XmnI GAAnn'nnTTC
Cuts at: 5081 5081
Size: 6952
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4760 276
Size: 53 83 186 936 3226 2468
Fragments arranged by size:
3226 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI
SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI
BstZ17I Bsu36I FalI FseI FspAI NruI NsiI PacI
PmlI PspXI PsrI RsrII SacII SexAI SwaI