Restriction map for
pFN21AE9694
(Circular) (Six-base) MAPSORT of: pFN21AE9694.seq Check: 191 from: 1 to: 5653
pFN21AE9694 5653 bp
With 182 enzymes: SgfI *
December 8, 2014 13:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3463 278
Size: 53 83 186 936 1927 2468
Fragments arranged by size:
2468 1927 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2060 2996 1937
Size: 123 936 4594
Fragments arranged by size:
4594 936 123
Acc65I G'GTAC_C
Cuts at: 2974 2974
Size: 5653
AclI AA'CG_TT
Cuts at: 1563 3780 4153 1563
Size: 2217 373 3063
Fragments arranged by size:
3063 2217 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2624 2801 3716 5100 5368 1632
Size: 992 177 915 1384 268 1917
Fragments arranged by size:
1917 1384 992 915 268 177
AflII C'TTAA_G
Cuts at: 820 1017 2924 820
Size: 197 1907 3549
Fragments arranged by size:
3549 1907 197
AflIII A'CryG_T
Cuts at: 1203 4552 5529 1203
Size: 3349 977 1327
Fragments arranged by size:
3349 1327 977
AgeI A'CCGG_T
Cuts at: 4484 4484
Size: 5653
AhdI GACnn_n'nnGTC
Cuts at: 4382 4382
Size: 5653
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5653
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5653
AlwNI CAG_nnn'CTG
Cuts at: 2153 3396 4968 2153
Size: 1243 1572 2838
Fragments arranged by size:
2838 1572 1243
ApaLI G'TGCA_C
Cuts at: 3710 4866 3710
Size: 1156 4497
ApoI r'AATT_y
Cuts at: 1493 2962 3188 3340 5449 1493
Size: 1469 226 152 2109 1697
Fragments arranged by size:
2109 1697 1469 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4207 160
Size: 872 3175 1606
Fragments arranged by size:
3175 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
AvaI C'yCGr_G
Cuts at: 1870 1943 2319 2978 1870
Size: 73 376 659 4545
Fragments arranged by size:
4545 659 376 73
BamHI G'GATC_C
Cuts at: 2983 3350 2983
Size: 367 5286
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2974 4429 618
Size: 325 217 627 1187 1455 1842
Fragments arranged by size:
1842 1455 1187 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2209 2529 2972 1391
Size: 818 320 443 4072
Fragments arranged by size:
4072 818 443 320
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4682
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3844 3878 1903
Size: 34 1907 34 3678
Fragments arranged by size:
3678 1907 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3545 4755 3545
Size: 1210 4443
BclI T'GATC_A
Cuts at: 1548 2734 1548
Size: 1186 4467
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4264 136
Size: 107 122 71 631 605 337 2255 1525
Fragments arranged by size:
2255 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5648 5648
Size: 5653
BlpI GC'TnA_GC
Cuts at: 2288 3070 2288
Size: 782 4871
Bme1580I G_kGCm'C
Cuts at: 1790 3714 4870 1790
Size: 1924 1156 2573
Fragments arranged by size:
2573 1924 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5653
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3384 4342 442
Size: 416 53 83 399 24 1967 958 1753
Fragments arranged by size:
1967 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5653
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2654 2864 4313 1239
Size: 1415 210 1449 2579
Fragments arranged by size:
2579 1449 1415 210
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2513 3778 4643 4941 2513
Size: 1265 865 298 3225
Fragments arranged by size:
3225 1265 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2696 4316 882
Size: 617 1197 1620 2219
Fragments arranged by size:
2219 1620 1197 617
BsaAI yAC'GTr
Cuts at: 493 1204 3444 493
Size: 711 2240 2702
Fragments arranged by size:
2702 2240 711
BsaBI GATnn'nnATC
Cuts at: 5647 5647
Size: 5653
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3460 3842 4477 275
Size: 53 83 186 936 1927 382 635 1451
Fragments arranged by size:
1927 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2097 4086 4484 4758 4905 5316 2097
Size: 1989 398 274 147 411 2434
Fragments arranged by size:
2434 1989 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5428
Fragments arranged by size:
5428 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2219 2269 2789 2831 1186
Size: 1033 50 520 42 4008
Fragments arranged by size:
4008 1033 520 50 42
BseYI C'CCAG_C
Cuts at: 4856 5352 4856
Size: 496 5157
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5080
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3103 3864 4892 1540
Size: 275 186 1102 761 1028 2301
Fragments arranged by size:
2301 1102 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2529 3714 3799 4870 2529
Size: 1185 85 1071 3312
Fragments arranged by size:
3312 1185 1071 85
BsmI GAATG_Cn'
Cuts at: 3164 3257 3164
Size: 93 5560
BsmBI CGTCTCn'nnnn_
Cuts at: 2422 2422
Size: 5653
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2209 2529 2972 3714 3799 4870 1391
Size: 399 419 320 443 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 443 419 399 320 85
BspEI T'CCGG_A
Cuts at: 5316 5316
Size: 5653
BspHI T'CATG_A
Cuts at: 3542 5272 3542
Size: 1730 3923
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3008 844
Size: 2164 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2292 3540 1394
Size: 429 469 1248 3507
Fragments arranged by size:
3507 1248 469 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4148 4322 65
Size: 1183 2900 174 1396
Fragments arranged by size:
2900 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2135 2199 2302 4297 4484 1700
Size: 110 325 64 103 1995 187 2869
Fragments arranged by size:
2869 1995 325 187 110 103 64
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5653
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5653
BssSI C'ACGA_G
Cuts at: 3713 4725 3713
Size: 1012 4641
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5653
BstBI TT'CG_AA
Cuts at: 4457 4457
Size: 5653
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5653
BstYI r'GATC_y
Cuts at: 1972 2983 3350 3735 3752 5204 5290 5302 5648
Size: 1011 367 385 17 1452 86 12 346
Cuts at: 5648 1972
Size: 1977
Fragments arranged by size:
1977 1452 1011 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4162
Fragments arranged by size:
4162 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4914
Fragments arranged by size:
4914 683 56
BtsI GCAGTG_nn'
Cuts at: 2460 3169 3963 3983 2460
Size: 709 794 20 4130
Fragments arranged by size:
4130 794 709 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5653
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5618
DraI TTT'AAA
Cuts at: 2169 2957 2169
Size: 788 4865
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4660 809
Size: 507 3344 1802
Fragments arranged by size:
3344 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2296 3100 3989
Size: 54 1425 50 275 18 466 804 889
Cuts at: 3989 5618 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 804 466 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3100 1537
Size: 275 1288 4090
Fragments arranged by size:
4090 1288 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2517 3583 1353
Size: 1164 1066 3423
Fragments arranged by size:
3423 1164 1066
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2076 4240 4614 4760 387
Size: 1064 138 487 2164 374 146 1280
Fragments arranged by size:
2164 1280 1064 487 374 146 138
EcoICRI GAG'CTC
Cuts at: 2527 2527
Size: 5653
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2624 2654 2801 2864 3716 4313 5100
Size: 393 992 30 147 63 852 597 787
Cuts at: 5100 5368 1239
Size: 268 1524
Fragments arranged by size:
1524 992 852 787 597 393 268 147
63 30
EcoO109I rG'GnC_Cy
Cuts at: 2608 2677 2608
Size: 69 5584
EcoRI G'AATT_C
Cuts at: 2962 3340 2962
Size: 378 5275
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2356 2356
Size: 5653
FspI TGC'GCA
Cuts at: 4159 4159
Size: 5653
HaeII r_GCGC'y
Cuts at: 1678 4800 1678
Size: 3122 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2575 2607 4294 4326 4368
Size: 32 135 32 1003 32 1687 32 42
Cuts at: 4368 4400 1373
Size: 32 2626
Fragments arranged by size:
2626 1687 1003 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2560 2583 2997 3243 669
Size: 988 281 622 23 414 246 3079
Fragments arranged by size:
3079 988 622 414 281 246 23
HindIII A'AGCT_T
Cuts at: 748 2783 748
Size: 2035 3618
HpaI GTT'AAC
Cuts at: 3243 3243
Size: 5653
KpnI G_GTAC'C
Cuts at: 2978 2978
Size: 5653
MfeI C'AATT_G
Cuts at: 3252 3252
Size: 5653
MluI A'CGCG_T
Cuts at: 5529 5529
Size: 5653
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4767 4951 1305
Size: 3462 184 2007
Fragments arranged by size:
3462 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3831
Fragments arranged by size:
3831 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3611 3970 4129 518
Size: 648 2445 359 159 2042
Fragments arranged by size:
2445 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2545 3069 3746 4894 5139 1606
Size: 939 524 677 1148 245 2120
Fragments arranged by size:
2120 1148 939 677 524 245
NaeI GCC'GGC
Cuts at: 2304 2304
Size: 5653
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4162
Fragments arranged by size:
4162 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5653
NgoMIV G'CCGG_C
Cuts at: 2302 2302
Size: 5653
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5653
NotI GC'GGCC_GC
Cuts at: 3100 3100
Size: 5653
NspI r_CATG'y
Cuts at: 1132 1229 3011 4556 1132
Size: 97 1782 1545 2229
Fragments arranged by size:
2229 1782 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5653
PciI A'CATG_T
Cuts at: 4552 4552
Size: 5653
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5653
PmeI GTTT'AAAC
Cuts at: 2957 2957
Size: 5653
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3722 3754 5235 5267 3722
Size: 32 1481 32 4108
Fragments arranged by size:
4108 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2608 2608
Size: 5653
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5653
PsiI TTA'TAA
Cuts at: 3223 3223
Size: 5653
PstI C_TGCA'G
Cuts at: 830 2158 2274 3005 830
Size: 1328 116 731 3478
Fragments arranged by size:
3478 1328 731 116
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5653
PvuII CAG'CTG
Cuts at: 2545 2545
Size: 5653
SacI G_AGCT'C
Cuts at: 2529 2529
Size: 5653
SalI G'TCGA_C
Cuts at: 1936 2995 1936
Size: 1059 4594
SapI GCTCTTCn'nnn_
Cuts at: 2517 2517
Size: 5653
SbfI CC_TGCA'GG
Cuts at: 3005 3005
Size: 5653
ScaI AGT'ACT
Cuts at: 3901 5613 3901
Size: 1712 3941
SfcI C'TryA_G
Cuts at: 826 1045 2061 2154 2270 3001 4136 4817 5008
Size: 219 1016 93 116 731 1135 681 191
Cuts at: 5008 826
Size: 1471
Fragments arranged by size:
1471 1135 1016 731 681 219 191 116
93
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5653
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5653
SmaI CCC'GGG
Cuts at: 1872 2980 1872
Size: 1108 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2528 2924 3757 4658 4920 820
Size: 197 926 585 396 833 901 262 1553
Fragments arranged by size:
1553 926 901 833 585 396 262 197
SnaBI TAC'GTA
Cuts at: 493 3444 493
Size: 2951 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5653
SphI G_CATG'C
Cuts at: 1132 3011 1132
Size: 1879 3774
SspI AAT'ATT
Cuts at: 5 52 3577 5
Size: 47 3525 2081
Fragments arranged by size:
3525 2081 47
StuI AGG'CCT
Cuts at: 1517 2828 1517
Size: 1311 4342
StyI C'CwwG_G
Cuts at: 513 1065 2004 3092 513
Size: 552 939 1088 3074
Fragments arranged by size:
3074 1088 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2094 3680 3697 3850 4035 4480 325
Size: 1255 514 1586 17 153 185 445 1498
Fragments arranged by size:
1586 1498 1255 514 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3899 5611 96
Size: 274 80 33 51 1443 1922 1712 138
Fragments arranged by size:
1922 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5613 5645 5613
Size: 32 5621
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5653
XbaI T'CTAG_A
Cuts at: 2989 2989
Size: 5653
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2809 2809
Size: 5653
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5653
XmaI C'CCGG_G
Cuts at: 1870 2978 1870
Size: 1108 4545
XmnI GAAnn'nnTTC
Cuts at: 3782 3782
Size: 5653
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3461 276
Size: 53 83 186 936 1927 2468
Fragments arranged by size:
2468 1927 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I
DraIII EcoNI EcoRV FseI FspAI KasI NarI NruI
NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII
SacII SanDI SexAI SfiI SfoI SrfI SwaI