Restriction map for
pFN21AE9690
(Circular) (Six-base) MAPSORT of: pFN21AE9690.seq Check: 6118 from: 1 to: 7660
pFN21AE9690 7660 bp
With 182 enzymes: SgfI *
December 8, 2014 13:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4849 5470 278
Size: 53 83 186 936 3313 621 2468
Fragments arranged by size:
3313 2468 936 621 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3928 5003 1937
Size: 1991 1075 4594
Fragments arranged by size:
4594 1991 1075
Acc65I G'GTAC_C
Cuts at: 3671 4981 3671
Size: 1310 6350
AclI AA'CG_TT
Cuts at: 1563 5787 6160 1563
Size: 4224 373 3063
Fragments arranged by size:
4224 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2451 2838 3267 3309 5723 7107 7375 1632
Size: 819 387 429 42 2414 1384 268 1917
Fragments arranged by size:
2414 1917 1384 819 429 387 268 42
AfeI AGC'GCT
Cuts at: 3229 3229
Size: 7660
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7463
AflIII A'CryG_T
Cuts at: 1203 6559 7536 1203
Size: 5356 977 1327
Fragments arranged by size:
5356 1327 977
AgeI A'CCGG_T
Cuts at: 6491 6491
Size: 7660
AhdI GACnn_n'nnGTC
Cuts at: 6389 6389
Size: 7660
AleI CACnn'nnGTG
Cuts at: 1166 2205 1166
Size: 1039 6621
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7660
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3040 3072 3040
Size: 32 7628
AlwNI CAG_nnn'CTG
Cuts at: 2576 4234 5403 6975 2576
Size: 1658 1169 1572 3261
Fragments arranged by size:
3261 1658 1572 1169
ApaLI G'TGCA_C
Cuts at: 2538 5717 6873 2538
Size: 3179 1156 3325
Fragments arranged by size:
3325 3179 1156
ApoI r'AATT_y
Cuts at: 1493 2192 4969 5195 5347 7456 1493
Size: 699 2777 226 152 2109 1697
Fragments arranged by size:
2777 2109 1697 699 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2172 2702 2955 6214 160
Size: 872 1140 530 253 3259 1606
Fragments arranged by size:
3259 1606 1140 872 530 253
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7660
AvaI C'yCGr_G
Cuts at: 1870 1943 3792 4985 1870
Size: 73 1849 1193 4545
Fragments arranged by size:
4545 1849 1193 73
AvrII C'CTAG_G
Cuts at: 2368 2368
Size: 7660
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3171 3204 3703 3736 3171
Size: 33 499 33 7095
Fragments arranged by size:
7095 499 33 33
BamHI G'GATC_C
Cuts at: 2109 4990 5357 2109
Size: 2881 367 4412
Fragments arranged by size:
4412 2881 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3671 4117 4981 6436 618
Size: 325 217 627 1884 446 864 1455 1842
Fragments arranged by size:
1884 1842 1455 864 627 446 325 217
BanII G_rGCy'C
Cuts at: 1391 2037 2130 4540 4979 1391
Size: 646 93 2410 439 4072
Fragments arranged by size:
4072 2410 646 439 93
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4791 4841 928
Size: 971 2892 50 3747
Fragments arranged by size:
3747 2892 971 50
BbvCI CC'TCA_GC
Cuts at: 2786 4164 2786
Size: 1378 6282
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5851 5885 1903
Size: 34 3914 34 3678
Fragments arranged by size:
3914 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5552 6762 5552
Size: 1210 6450
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7660
BfrBI ATG'CAT
Cuts at: 3491 3491
Size: 7660
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3130 6271 136
Size: 107 122 71 631 605 1458 3141 1525
Fragments arranged by size:
3141 1525 1458 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3038 7655 3038
Size: 4617 3043
BlpI GC'TnA_GC
Cuts at: 5077 5077
Size: 7660
Bme1580I G_kGCm'C
Cuts at: 1790 2542 4306 4916 5721 6877 1790
Size: 752 1764 610 805 1156 2573
Fragments arranged by size:
2573 1764 1156 805 752 610
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7660
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3578 4439 5391
Size: 416 53 83 399 24 2161 861 952
Cuts at: 5391 6349 442
Size: 958 1753
Fragments arranged by size:
2161 1753 958 952 861 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7660
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3286 3286
Size: 7660
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3019 4206 4392 4464 6320 1239
Size: 1780 1187 186 72 1856 2579
Fragments arranged by size:
2579 1856 1780 1187 186 72
Bpu10I CC'TnA_GC
Cuts at: 2786 3707 4164 2786
Size: 921 457 6282
Fragments arranged by size:
6282 921 457
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3148 3361 4023 5785 6650 6948 3148
Size: 213 662 1762 865 298 3860
Fragments arranged by size:
3860 1762 865 662 298 213
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2550 3018 6323 882
Size: 617 1051 468 3305 2219
Fragments arranged by size:
3305 2219 1051 617 468
BsaAI yAC'GTr
Cuts at: 493 1204 5451 493
Size: 711 4247 2702
Fragments arranged by size:
4247 2702 711
BsaBI GATnn'nnATC
Cuts at: 7654 7654
Size: 7660
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4846 5467 5849 6484
Size: 53 83 186 936 3313 621 382 635
Cuts at: 6484 275
Size: 1451
Fragments arranged by size:
3313 1451 936 635 621 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 6093 6491 6765 6912 7323 6093
Size: 398 274 147 411 6430
Fragments arranged by size:
6430 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3040 3070 3400 3430 4015
Size: 30 165 30 1637 30 330 30 585
Cuts at: 4015 4045 4204 4234 1178
Size: 30 159 30 4604
Fragments arranged by size:
4604 1637 585 330 165 159 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4008 4291 4500 1186
Size: 2822 283 209 4346
Fragments arranged by size:
4346 2822 283 209
BseYI C'CCAG_C
Cuts at: 3450 3574 3888 3894 4941 6863 7359 3450
Size: 124 314 6 1047 1922 496 3751
Fragments arranged by size:
3751 1922 1047 496 314 124 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2339 4137 4733 1163
Size: 573 603 1798 596 4090
Fragments arranged by size:
4090 1798 603 596 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5110 5871 6899 1540
Size: 275 186 3109 761 1028 2301
Fragments arranged by size:
3109 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2037 2542 4540 5721 5806 6877 2037
Size: 505 1998 1181 85 1071 2820
Fragments arranged by size:
2820 1998 1181 1071 505 85
BsmI GAATG_Cn'
Cuts at: 2521 3142 3997 5171 5264 2521
Size: 621 855 1174 93 4917
Fragments arranged by size:
4917 1174 855 621 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4837 4837
Size: 7660
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2037 2130 2542 4306 4540 4916 4979
Size: 399 247 93 412 1764 234 376 63
Cuts at: 4979 5721 5806 6877 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1764 1071 742 412 399 376 247
234 93 85 63
BspEI T'CCGG_A
Cuts at: 7323 7323
Size: 7660
BspHI T'CATG_A
Cuts at: 3682 4243 5549 7279 3682
Size: 561 1306 1730 4063
Fragments arranged by size:
4063 1730 1306 561
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5015 844
Size: 4171 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5547 1394
Size: 429 3724 3507
Fragments arranged by size:
3724 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3429 3692 4475 4681 6155 6329 65
Size: 1183 2181 263 783 206 1474 174 1396
Fragments arranged by size:
2181 1474 1396 1183 783 263 206 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6304 6491 1700
Size: 110 4494 187 2869
Fragments arranged by size:
4494 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4066 4715 96
Size: 3970 649 3041
Fragments arranged by size:
3970 3041 649
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7660
BssSI C'ACGA_G
Cuts at: 5720 6732 5720
Size: 1012 6648
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7660
BstBI TT'CG_AA
Cuts at: 6464 6464
Size: 7660
BstEII G'GTnAC_C
Cuts at: 1184 2243 3432 3530 1184
Size: 1059 1189 98 5314
Fragments arranged by size:
5314 1189 1059 98
BstXI CCAn_nnnn'nTGG
Cuts at: 2303 2303
Size: 7660
BstYI r'GATC_y
Cuts at: 1972 2109 3038 4990 5357 5742 5759 7211 7297
Size: 137 929 1952 367 385 17 1452 86
Cuts at: 7297 7309 7655 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1952 1452 929 385 367 346 137
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2881 2881
Size: 7660
BtgI C'CryG_G
Cuts at: 513 1065 3089 513
Size: 552 2024 5084
Fragments arranged by size:
5084 2024 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6921
Fragments arranged by size:
6921 683 56
BtsI GCAGTG_nn'
Cuts at: 2797 4750 5176 5970 5990 2797
Size: 1953 426 794 20 4467
Fragments arranged by size:
4467 1953 794 426 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7660
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7625
DraI TTT'AAA
Cuts at: 4964 4964
Size: 7660
DraIII CAC_nnn'GTG
Cuts at: 2381 3464 3650 4472 4876 2381
Size: 1083 186 822 404 5165
Fragments arranged by size:
5165 1083 822 404 186
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6667 809
Size: 507 5351 1802
Fragments arranged by size:
5351 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3077 3808 5107
Size: 54 1425 50 275 18 1247 731 1299
Cuts at: 5107 5996 7625 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1299 1247 889 731 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5107 1537
Size: 275 3295 4090
Fragments arranged by size:
4090 3295 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2054 2806 4545 5590 1353
Size: 701 752 1739 1045 3423
Fragments arranged by size:
3423 1739 1045 752 701
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4023 6247 6621 6767 387
Size: 1064 138 2434 2224 374 146 1280
Fragments arranged by size:
2434 2224 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2035 4538 2035
Size: 2503 5157
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2451 2838 3019 3267 3309 4206 4392
Size: 393 819 387 181 248 42 897 186
Cuts at: 4392 4464 5723 6320 7107 7375 1239
Size: 72 1259 597 787 268 1524
Fragments arranged by size:
1524 1259 897 819 787 597 393 387
268 248 186 181 72 42
EcoNI CCTnn'n_nnAGG
Cuts at: 2671 2671
Size: 7660
EcoO109I rG'GnC_Cy
Cuts at: 4622 4622
Size: 7660
EcoRI G'AATT_C
Cuts at: 2192 4969 5347 2192
Size: 2777 378 4505
Fragments arranged by size:
4505 2777 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2129 3453 3858 2129
Size: 1324 405 5931
Fragments arranged by size:
5931 1324 405
FspI TGC'GCA
Cuts at: 3882 6166 3882
Size: 2284 5376
HaeII r_GCGC'y
Cuts at: 1678 3231 6807 1678
Size: 1553 3576 2531
Fragments arranged by size:
3576 2531 1553
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2558 2590 3786 3818 4015
Size: 32 135 32 986 32 1196 32 197
Cuts at: 4015 4047 4222 4254 4276 4308 4595 4627 6301
Size: 32 175 32 22 32 287 32 1674
Cuts at: 6301 6333 6375 6407 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1674 1196 986 287 197 175 135
42 32 32 32 32 32 32 32
32 32 32 22
HincII GTy'rAC
Cuts at: 669 1657 1938 3839 3929 4885 5004 5250 669
Size: 988 281 1901 90 956 119 246 3079
Fragments arranged by size:
3079 1901 988 956 281 246 119 90
HindIII A'AGCT_T
Cuts at: 748 2916 3981 748
Size: 2168 1065 4427
Fragments arranged by size:
4427 2168 1065
HpaI GTT'AAC
Cuts at: 5250 5250
Size: 7660
KpnI G_GTAC'C
Cuts at: 3675 4985 3675
Size: 1310 6350
MfeI C'AATT_G
Cuts at: 4704 5259 4704
Size: 555 7105
MluI A'CGCG_T
Cuts at: 7536 7536
Size: 7660
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2977 4319 6774 6958 1305
Size: 1672 1342 2455 184 2007
Fragments arranged by size:
2455 2007 1672 1342 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3810 10
Size: 54 1425 343 1978 3860
Fragments arranged by size:
3860 1978 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2205 3735 4002 4242 4368 5618 5977
Size: 648 1039 1530 267 240 126 1250 359
Cuts at: 5977 6136 518
Size: 159 2042
Fragments arranged by size:
2042 1530 1250 1039 648 359 267 240
159 126
MspA1I CmG'CkG
Cuts at: 1606 2873 3287 3578 3821 4151 4945 5076 5753
Size: 1267 414 291 243 330 794 131 677
Cuts at: 5753 6901 7146 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1267 1148 794 677 414 330 291
245 243 131
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 7108
NdeI CA'TA_TG
Cuts at: 387 4919 387
Size: 4532 3128
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7660
NotI GC'GGCC_GC
Cuts at: 5107 5107
Size: 7660
NsiI A_TGCA'T
Cuts at: 3493 3493
Size: 7660
NspI r_CATG'y
Cuts at: 1132 1229 3940 4073 5018 6563 1132
Size: 97 2711 133 945 1545 2229
Fragments arranged by size:
2711 2229 1545 945 133 97
PasI CC'CwG_GG
Cuts at: 1348 4696 1348
Size: 3348 4312
PciI A'CATG_T
Cuts at: 6559 6559
Size: 7660
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4349 1108
Size: 3241 4419
PmeI GTTT'AAAC
Cuts at: 4964 4964
Size: 7660
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3040 3072 5729 5761 7242 7274 3040
Size: 32 2657 32 1481 32 3426
Fragments arranged by size:
3426 2657 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4622 4622
Size: 7660
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7660
PsiI TTA'TAA
Cuts at: 5230 5230
Size: 7660
PstI C_TGCA'G
Cuts at: 830 2158 4456 5012 830
Size: 1328 2298 556 3478
Fragments arranged by size:
3478 2298 1328 556
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7660
PvuII CAG'CTG
Cuts at: 2873 3287 3578 4151 4945 2873
Size: 414 291 573 794 5588
Fragments arranged by size:
5588 794 573 414 291
SacI G_AGCT'C
Cuts at: 2037 4540 2037
Size: 2503 5157
SalI G'TCGA_C
Cuts at: 1936 3927 5002 1936
Size: 1991 1075 4594
Fragments arranged by size:
4594 1991 1075
SapI GCTCTTCn'nnn_
Cuts at: 4545 4545
Size: 7660
SbfI CC_TGCA'GG
Cuts at: 2158 5012 2158
Size: 2854 4806
ScaI AGT'ACT
Cuts at: 5908 7620 5908
Size: 1712 5948
SexAI A'CCwGG_T
Cuts at: 4468 4468
Size: 7660
SfcI C'TryA_G
Cuts at: 826 1045 2154 3183 3363 4452 5008 6143 6824
Size: 219 1109 1029 180 1089 556 1135 681
Cuts at: 6824 7015 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1109 1089 1029 681 556 219
191 180
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7660
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7660
SmaI CCC'GGG
Cuts at: 1872 4987 1872
Size: 3115 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3127 3376 4038 5764 6665 6927
Size: 197 926 1184 249 662 1726 901 262
Cuts at: 6927 820
Size: 1553
Fragments arranged by size:
1726 1553 1184 926 901 662 262 249
197
SnaBI TAC'GTA
Cuts at: 493 5451 493
Size: 4958 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7660
SphI G_CATG'C
Cuts at: 1132 5018 1132
Size: 3886 3774
SspI AAT'ATT
Cuts at: 5 52 2349 5584 5
Size: 47 2297 3235 2081
Fragments arranged by size:
3235 2297 2081 47
StuI AGG'CCT
Cuts at: 1517 3269 1517
Size: 1752 5908
StyI C'CwwG_G
Cuts at: 513 1065 2368 3025 3428 5099 513
Size: 552 1303 657 403 1671 3074
Fragments arranged by size:
3074 1671 1303 657 552 403
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3000 3588 4356 5687 5704 5857 6042
Size: 1255 1420 588 768 1331 17 153 185
Cuts at: 6042 6487 325
Size: 445 1498
Fragments arranged by size:
1498 1420 1331 1255 768 588 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3637 4066 4145
Size: 274 80 33 51 1443 1660 429 79
Cuts at: 4145 4715 4802 5906 7618 96
Size: 570 87 1104 1712 138
Fragments arranged by size:
1712 1660 1443 1104 570 429 274 138
87 80 79 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2365 2397 4204 4236 7620 7652 2365
Size: 32 1807 32 3384 32 2373
Fragments arranged by size:
3384 2373 1807 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 4384 1316
Size: 3068 4592
XbaI T'CTAG_A
Cuts at: 4996 4996
Size: 7660
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4924 4924
Size: 7660
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7660
XmaI C'CCGG_G
Cuts at: 1870 4985 1870
Size: 3115 4545
XmnI GAAnn'nnTTC
Cuts at: 2192 4650 5789 2192
Size: 2458 1139 4063
Fragments arranged by size:
4063 2458 1139
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4847 5468 276
Size: 53 83 186 936 3313 621 2468
Fragments arranged by size:
3313 2468 936 621 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI ApaI AscI BbeI BsiWI BstZ17I EcoRV FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI
SfoI SrfI SwaI