Restriction map for
pFN21AE9689
(Circular) (Six-base) MAPSORT of: pFN21AE9689.seq Check: 2214 from: 1 to: 6175
pFN21AE9689 6175 bp
With 182 enzymes: SgfI *
December 8, 2014 13:56 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3985 278
Size: 53 83 186 936 2449 2468
Fragments arranged by size:
2468 2449 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3518 1937
Size: 1581 4594
Acc65I G'GTAC_C
Cuts at: 3496 3496
Size: 6175
AclI AA'CG_TT
Cuts at: 1563 2817 4302 4675 1563
Size: 1254 1485 373 3063
Fragments arranged by size:
3063 1485 1254 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3194 3344 4238 5622 5890 1632
Size: 1562 150 894 1384 268 1917
Fragments arranged by size:
1917 1562 1384 894 268 150
AflII C'TTAA_G
Cuts at: 820 1017 3319 820
Size: 197 2302 3676
Fragments arranged by size:
3676 2302 197
AflIII A'CryG_T
Cuts at: 1203 5074 6051 1203
Size: 3871 977 1327
Fragments arranged by size:
3871 1327 977
AgeI A'CCGG_T
Cuts at: 5006 5006
Size: 6175
AhdI GACnn_n'nnGTC
Cuts at: 4904 4904
Size: 6175
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6175
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2535 1184
Size: 1351 4824
AlwNI CAG_nnn'CTG
Cuts at: 2759 3918 5490 2759
Size: 1159 1572 3444
Fragments arranged by size:
3444 1572 1159
ApaLI G'TGCA_C
Cuts at: 3339 4232 5388 3339
Size: 893 1156 4126
Fragments arranged by size:
4126 1156 893
ApoI r'AATT_y
Cuts at: 1493 2008 2543 3461 3484 3710 3862 5971 1493
Size: 515 535 918 23 226 152 2109 1697
Fragments arranged by size:
2109 1697 918 535 515 226 152 23
AseI AT'TA_AT
Cuts at: 160 1032 4729 160
Size: 872 3697 1606
Fragments arranged by size:
3697 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6175
AvaI C'yCGr_G
Cuts at: 1870 1943 2400 3500 1870
Size: 73 457 1100 4545
Fragments arranged by size:
4545 1100 457 73
BamHI G'GATC_C
Cuts at: 3505 3872 3505
Size: 367 5808
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3496 4951 618
Size: 325 217 627 1709 1455 1842
Fragments arranged by size:
1842 1709 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2332 2401 3494 1391
Size: 941 69 1093 4072
Fragments arranged by size:
4072 1093 941 69
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2817 928
Size: 971 918 4286
Fragments arranged by size:
4286 971 918
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2150 2184 4366 4400 1903
Size: 34 213 34 2182 34 3678
Fragments arranged by size:
3678 2182 213 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2611 4067 5277 2611
Size: 1456 1210 3509
Fragments arranged by size:
3509 1456 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6175
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4786 136
Size: 107 122 71 631 605 3114 1525
Fragments arranged by size:
3114 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2638 6170 2638
Size: 3532 2643
BlpI GC'TnA_GC
Cuts at: 3165 3592 3165
Size: 427 5748
Bme1580I G_kGCm'C
Cuts at: 1790 3343 4236 5392 1790
Size: 1553 893 1156 2573
Fragments arranged by size:
2573 1553 1156 893
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6175
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3906 4864 442
Size: 416 53 83 399 24 2489 958 1753
Fragments arranged by size:
2489 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2777 1056
Size: 1721 4454
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2232 4835 1239
Size: 993 2603 2579
Fragments arranged by size:
2603 2579 993
Bpu10I CC'TnA_GC
Cuts at: 2663 2663
Size: 6175
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4300 5165 5463 4300
Size: 865 298 5012
Fragments arranged by size:
5012 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4838 882
Size: 617 3339 2219
Fragments arranged by size:
3339 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3966 493
Size: 711 2762 2702
Fragments arranged by size:
2762 2702 711
BsaBI GATnn'nnATC
Cuts at: 2568 6169 2568
Size: 3601 2574
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2347 3982 4364 4999
Size: 53 83 186 936 814 1635 382 635
Cuts at: 4999 275
Size: 1451
Fragments arranged by size:
1635 1451 936 814 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4608 5006 5280 5427 5838 4608
Size: 398 274 147 411 4945
Fragments arranged by size:
4945 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5950
Fragments arranged by size:
5950 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3039 1186
Size: 1853 4322
BseYI C'CCAG_C
Cuts at: 5378 5874 5378
Size: 496 5679
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5602
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3625 4386 5414 1540
Size: 275 186 1624 761 1028 2301
Fragments arranged by size:
2301 1624 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2332 2422 3343 4236 4321 5392 2332
Size: 90 921 893 85 1071 3115
Fragments arranged by size:
3115 1071 921 893 90 85
BsmI GAATG_Cn'
Cuts at: 2616 3686 3779 2616
Size: 1070 93 5012
Fragments arranged by size:
5012 1070 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3295 3295
Size: 6175
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2332 2401 2422 3343 3494 4236 4321
Size: 399 542 69 21 921 151 742 85
Cuts at: 4321 5392 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 921 742 542 399 151 85
69 21
BspEI T'CCGG_A
Cuts at: 5838 5838
Size: 6175
BspHI T'CATG_A
Cuts at: 4064 5794 4064
Size: 1730 4445
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3530 844
Size: 2686 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4062 1394
Size: 429 2239 3507
Fragments arranged by size:
3507 2239 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2119 3303 4670 4844 65
Size: 1183 871 1184 1367 174 1396
Fragments arranged by size:
1396 1367 1184 1183 871 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4819 5006 1700
Size: 110 3009 187 2869
Fragments arranged by size:
3009 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6175
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6175
BssSI C'ACGA_G
Cuts at: 4235 5247 4235
Size: 1012 5163
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6175
BstBI TT'CG_AA
Cuts at: 4979 4979
Size: 6175
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6175
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2011
Size: 6175
BstYI r'GATC_y
Cuts at: 1972 2137 2287 2569 2638 3202 3467 3505 3872
Size: 165 150 282 69 564 265 38 367
Cuts at: 3872 4257 4274 5726 5812 5824 6170 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 564 385 367 346 282 265
165 150 86 69 38 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2723 3086 513
Size: 552 1658 363 3602
Fragments arranged by size:
3602 1658 552 363
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5436
Fragments arranged by size:
5436 683 56
BtsI GCAGTG_nn'
Cuts at: 3691 4485 4505 3691
Size: 794 20 5361
Fragments arranged by size:
5361 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6175
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6140
DraI TTT'AAA
Cuts at: 2233 3479 2233
Size: 1246 4929
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5182 809
Size: 507 3866 1802
Fragments arranged by size:
3866 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2300 3622 4511
Size: 54 1425 50 275 18 470 1322 889
Cuts at: 4511 6140 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1322 889 470 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3622 1537
Size: 275 1810 4090
Fragments arranged by size:
4090 1810 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2038 2944 3154 4105 1353
Size: 685 906 210 951 3423
Fragments arranged by size:
3423 951 906 685 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4762 5136 5282 387
Size: 1064 138 3173 374 146 1280
Fragments arranged by size:
3173 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2330 2330
Size: 6175
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2232 3194 3344 4238 4835 5622 5890
Size: 393 600 962 150 894 597 787 268
Cuts at: 5890 1239
Size: 1524
Fragments arranged by size:
1524 962 894 787 600 597 393 268
150
EcoNI CCTnn'n_nnAGG
Cuts at: 3188 3188
Size: 6175
EcoO109I rG'GnC_Cy
Cuts at: 2405 2405
Size: 6175
EcoRI G'AATT_C
Cuts at: 3484 3862 3484
Size: 378 5797
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3374 3374
Size: 6175
FspI TGC'GCA
Cuts at: 4681 4681
Size: 6175
HaeII r_GCGC'y
Cuts at: 1678 5322 1678
Size: 3644 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2332 2364 2585 2617 3294
Size: 32 135 32 760 32 221 32 677
Cuts at: 3294 3326 4816 4848 4890 4922 1373
Size: 32 1490 32 42 32 2626
Fragments arranged by size:
2626 1490 760 677 221 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3519 3765 669
Size: 988 281 1581 246 3079
Fragments arranged by size:
3079 1581 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3102 748
Size: 2354 3821
HpaI GTT'AAC
Cuts at: 3765 3765
Size: 6175
KpnI G_GTAC'C
Cuts at: 3500 3500
Size: 6175
MfeI C'AATT_G
Cuts at: 3774 3774
Size: 6175
MluI A'CGCG_T
Cuts at: 6051 6051
Size: 6175
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2725 3001 5289 5473 1305
Size: 1420 276 2288 184 2007
Fragments arranged by size:
2288 2007 1420 276 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2302 10
Size: 54 1425 343 470 3883
Fragments arranged by size:
3883 1425 470 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2625 3008 4133 4492 4651 518
Size: 648 1459 383 1125 359 159 2042
Fragments arranged by size:
2042 1459 1125 648 383 359 159
MspA1I CmG'CkG
Cuts at: 1606 3591 4268 5416 5661 1606
Size: 1985 677 1148 245 2120
Fragments arranged by size:
2120 1985 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2723 513
Size: 552 1658 3965
Fragments arranged by size:
3965 1658 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6175
NheI G'CTAG_C
Cuts at: 1052 2773 1052
Size: 1721 4454
NotI GC'GGCC_GC
Cuts at: 3622 3622
Size: 6175
NspI r_CATG'y
Cuts at: 1132 1229 3533 5078 1132
Size: 97 2304 1545 2229
Fragments arranged by size:
2304 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6175
PciI A'CATG_T
Cuts at: 5074 5074
Size: 6175
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2759 1108
Size: 1651 4524
PfoI T'CCnGG_A
Cuts at: 2603 2603
Size: 6175
PmeI GTTT'AAAC
Cuts at: 3479 3479
Size: 6175
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4244 4276 5757 5789 4244
Size: 32 1481 32 4630
Fragments arranged by size:
4630 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2405 2405
Size: 6175
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6175
PsiI TTA'TAA
Cuts at: 3745 3745
Size: 6175
PstI C_TGCA'G
Cuts at: 830 2320 2945 3430 3527 830
Size: 1490 625 485 97 3478
Fragments arranged by size:
3478 1490 625 485 97
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6175
SacI G_AGCT'C
Cuts at: 2332 2332
Size: 6175
SalI G'TCGA_C
Cuts at: 1936 3517 1936
Size: 1581 4594
SapI GCTCTTCn'nnn_
Cuts at: 3154 3154
Size: 6175
SbfI CC_TGCA'GG
Cuts at: 3527 3527
Size: 6175
ScaI AGT'ACT
Cuts at: 4423 6135 4423
Size: 1712 4463
SfcI C'TryA_G
Cuts at: 826 1045 2316 2409 2470 2941 3426 3523 4658
Size: 219 1271 93 61 471 485 97 1135
Cuts at: 4658 5339 5530 826
Size: 681 191 1471
Fragments arranged by size:
1471 1271 1135 681 485 471 219 191
97 93 61
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6175
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6175
SmaI CCC'GGG
Cuts at: 1872 2402 3502 1872
Size: 530 1100 4545
Fragments arranged by size:
4545 1100 530
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3319 4279 5180 5442 820
Size: 197 926 1376 960 901 262 1553
Fragments arranged by size:
1553 1376 960 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3966 493
Size: 3473 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6175
SphI G_CATG'C
Cuts at: 1132 3533 1132
Size: 2401 3774
SspI AAT'ATT
Cuts at: 5 52 4099 5
Size: 47 4047 2081
Fragments arranged by size:
4047 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6175
StyI C'CwwG_G
Cuts at: 513 1065 2723 3614 513
Size: 552 1658 891 3074
Fragments arranged by size:
3074 1658 891 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4202 4219 4372 4557 5002 325
Size: 1255 2622 17 153 185 445 1498
Fragments arranged by size:
2622 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2734 4421 6133
Size: 274 80 33 51 1443 757 1687 1712
Cuts at: 6133 96
Size: 138
Fragments arranged by size:
1712 1687 1443 757 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6135 6167 6135
Size: 32 6143
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6175
XbaI T'CTAG_A
Cuts at: 3511 3511
Size: 6175
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3058 3058
Size: 6175
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6175
XmaI C'CCGG_G
Cuts at: 1870 2400 3500 1870
Size: 530 1100 4545
Fragments arranged by size:
4545 1100 530
XmnI GAAnn'nnTTC
Cuts at: 4304 4304
Size: 6175
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3983 276
Size: 53 83 186 936 2449 2468
Fragments arranged by size:
2468 2449 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI