Restriction map for
pFN21AE9687
(Circular) (Six-base) MAPSORT of: pFN21AE9687.seq Check: 4324 from: 1 to: 5392
pFN21AE9687 5392 bp
With 182 enzymes: SgfI *
December 8, 2014 13:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2119 2119
Size: 5392
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3202 278
Size: 53 83 186 936 1666 2468
Fragments arranged by size:
2468 1666 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2735 1937
Size: 798 4594
Acc65I G'GTAC_C
Cuts at: 2713 2713
Size: 5392
AclI AA'CG_TT
Cuts at: 1563 2691 3519 3892 1563
Size: 1128 828 373 3063
Fragments arranged by size:
3063 1128 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3455 4839 5107 1632
Size: 1823 1384 268 1917
Fragments arranged by size:
1917 1823 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5195
AflIII A'CryG_T
Cuts at: 1203 4291 5268 1203
Size: 3088 977 1327
Fragments arranged by size:
3088 1327 977
AgeI A'CCGG_T
Cuts at: 4223 4223
Size: 5392
AhdI GACnn_n'nnGTC
Cuts at: 4121 4121
Size: 5392
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5392
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5392
AlwNI CAG_nnn'CTG
Cuts at: 2020 3135 4707 2020
Size: 1115 1572 2705
Fragments arranged by size:
2705 1572 1115
ApaLI G'TGCA_C
Cuts at: 2276 3449 4605 2276
Size: 1173 1156 3063
Fragments arranged by size:
3063 1173 1156
ApoI r'AATT_y
Cuts at: 1493 2701 2927 3079 5188 1493
Size: 1208 226 152 2109 1697
Fragments arranged by size:
2109 1697 1208 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3946 160
Size: 872 2914 1606
Fragments arranged by size:
2914 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5392
AvaI C'yCGr_G
Cuts at: 1870 1943 2052 2306 2717 1870
Size: 73 109 254 411 4545
Fragments arranged by size:
4545 411 254 109 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2115 2148 2115
Size: 33 5359
BamHI G'GATC_C
Cuts at: 2722 3089 2722
Size: 367 5025
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2713 4168 618
Size: 325 217 627 926 1455 1842
Fragments arranged by size:
1842 1455 926 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2711 1391
Size: 1320 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2311 928
Size: 971 412 4009
Fragments arranged by size:
4009 971 412
BbvCI CC'TCA_GC
Cuts at: 2175 2175
Size: 5392
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3583 3617 1903
Size: 34 1646 34 3678
Fragments arranged by size:
3678 1646 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2116 3284 4494 2116
Size: 1168 1210 3014
Fragments arranged by size:
3014 1210 1168
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5392
BfrBI ATG'CAT
Cuts at: 2549 2549
Size: 5392
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2023 2209
Size: 107 122 71 631 605 337 14 186
Cuts at: 2209 4003 136
Size: 1794 1525
Fragments arranged by size:
1794 1525 631 605 337 186 122 107
71 14
BglII A'GATC_T
Cuts at: 5387 5387
Size: 5392
BlpI GC'TnA_GC
Cuts at: 2013 2809 2013
Size: 796 4596
Bme1580I G_kGCm'C
Cuts at: 1790 2280 3453 4609 1790
Size: 490 1173 1156 2573
Fragments arranged by size:
2573 1173 1156 490
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5392
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3123 4081 442
Size: 416 53 83 399 24 1706 958 1753
Fragments arranged by size:
1753 1706 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5392
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2308 4052 1239
Size: 1069 1744 2579
Fragments arranged by size:
2579 1744 1069
Bpu10I CC'TnA_GC
Cuts at: 2175 2175
Size: 5392
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2497 3517 4382 4680 2497
Size: 1020 865 298 3209
Fragments arranged by size:
3209 1020 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4055 882
Size: 617 2556 2219
Fragments arranged by size:
2556 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3183 493
Size: 711 1979 2702
Fragments arranged by size:
2702 1979 711
BsaBI GATnn'nnATC
Cuts at: 5386 5386
Size: 5392
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3199 3581 4216 275
Size: 53 83 186 936 1666 382 635 1451
Fragments arranged by size:
1666 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3825 4223 4497 4644 5055 3825
Size: 398 274 147 411 4162
Fragments arranged by size:
4162 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5167
Fragments arranged by size:
5167 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2192 1186
Size: 1006 4386
BseYI C'CCAG_C
Cuts at: 2167 4595 5091 2167
Size: 2428 496 2468
Fragments arranged by size:
2468 2428 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4819
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2842 3603 4631 1540
Size: 275 186 841 761 1028 2301
Fragments arranged by size:
2301 1028 841 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2265 2280 3453 3538 4609 2265
Size: 15 1173 85 1071 3048
Fragments arranged by size:
3048 1173 1071 85 15
BsmI GAATG_Cn'
Cuts at: 2903 2996 2903
Size: 93 5299
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2265 2280 2711 3453 3538 4609 1391
Size: 399 475 15 431 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 475 431 399 85 15
BspEI T'CCGG_A
Cuts at: 5055 5055
Size: 5392
BspHI T'CATG_A
Cuts at: 3281 5011 3281
Size: 1730 3662
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2119 2747 844
Size: 1275 628 3489
Fragments arranged by size:
3489 1275 628
BsrBI CCG'CTC
Cuts at: 1394 1823 2152 3279 1394
Size: 429 329 1127 3507
Fragments arranged by size:
3507 1127 429 329
BsrDI GCAATG_nn'
Cuts at: 65 1248 3887 4061 65
Size: 1183 2639 174 1396
Fragments arranged by size:
2639 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2189 4036 4223 1700
Size: 110 379 1847 187 2869
Fragments arranged by size:
2869 1847 379 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5392
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5392
BssSI C'ACGA_G
Cuts at: 3452 4464 3452
Size: 1012 4380
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5392
BstBI TT'CG_AA
Cuts at: 4196 4196
Size: 5392
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5392
BstYI r'GATC_y
Cuts at: 1972 2722 3089 3474 3491 4943 5029 5041 5387
Size: 750 367 385 17 1452 86 12 346
Cuts at: 5387 1972
Size: 1977
Fragments arranged by size:
1977 1452 750 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2112 2313 513
Size: 552 939 108 201 3592
Fragments arranged by size:
3592 939 552 201 108
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2012 487
Size: 683 56 786 3867
Fragments arranged by size:
3867 786 683 56
BtsI GCAGTG_nn'
Cuts at: 2265 2908 3702 3722 2265
Size: 643 794 20 3935
Fragments arranged by size:
3935 794 643 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5392
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5357
DraI TTT'AAA
Cuts at: 2696 2696
Size: 5392
DraIII CAC_nnn'GTG
Cuts at: 2039 2260 2039
Size: 221 5171
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4399 809
Size: 507 3083 1802
Fragments arranged by size:
3083 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2839 3728 5357
Size: 54 1425 50 275 18 1009 889 1629
Cuts at: 5357 8
Size: 43
Fragments arranged by size:
1629 1425 1009 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2839 1537
Size: 275 1027 4090
Fragments arranged by size:
4090 1027 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3322 1353
Size: 1969 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3979 4353 4499 387
Size: 1064 138 2390 374 146 1280
Fragments arranged by size:
2390 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2308 3455 4052 4839 5107 1239
Size: 393 676 1147 597 787 268 1524
Fragments arranged by size:
1524 1147 787 676 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2142 2290 2142
Size: 148 5244
EcoRI G'AATT_C
Cuts at: 2701 3079 2701
Size: 378 5014
FspI TGC'GCA
Cuts at: 2245 3898 2245
Size: 1653 3739
FspAI rTGC'GCAy
Cuts at: 2245 2245
Size: 5392
HaeII r_GCGC'y
Cuts at: 1678 2357 4539 1678
Size: 679 2182 2531
Fragments arranged by size:
2531 2182 679
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4033 4065 4107 4139 1373
Size: 32 135 32 2461 32 42 32 2626
Fragments arranged by size:
2626 2461 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2736 2982 669
Size: 988 281 798 246 3079
Fragments arranged by size:
3079 988 798 281 246
HindIII A'AGCT_T
Cuts at: 748 2094 748
Size: 1346 4046
HpaI GTT'AAC
Cuts at: 2982 2982
Size: 5392
KpnI G_GTAC'C
Cuts at: 2717 2717
Size: 5392
MfeI C'AATT_G
Cuts at: 2991 2991
Size: 5392
MluI A'CGCG_T
Cuts at: 5268 5268
Size: 5392
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4506 4690 1305
Size: 3201 184 2007
Fragments arranged by size:
3201 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3570
Fragments arranged by size:
3570 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3350 3709 3868 518
Size: 648 2184 359 159 2042
Fragments arranged by size:
2184 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2020 2200 2808 3485 4633 4878 1606
Size: 414 180 608 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 608 414 245 180
NaeI GCC'GGC
Cuts at: 2191 2191
Size: 5392
NcoI C'CATG_G
Cuts at: 513 1065 2004 2112 513
Size: 552 939 108 3793
Fragments arranged by size:
3793 939 552 108
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5392
NgoMIV G'CCGG_C
Cuts at: 2189 2189
Size: 5392
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5392
NotI GC'GGCC_GC
Cuts at: 2839 2839
Size: 5392
NsiI A_TGCA'T
Cuts at: 2551 2551
Size: 5392
NspI r_CATG'y
Cuts at: 1132 1229 2750 4295 1132
Size: 97 1521 1545 2229
Fragments arranged by size:
2229 1545 1521 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5392
PciI A'CATG_T
Cuts at: 4291 4291
Size: 5392
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2118 1108
Size: 1010 4382
PmeI GTTT'AAAC
Cuts at: 2696 2696
Size: 5392
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3461 3493 4974 5006 3461
Size: 32 1481 32 3847
Fragments arranged by size:
3847 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2142 2290 2142
Size: 148 5244
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5392
PsiI TTA'TAA
Cuts at: 2962 2962
Size: 5392
PstI C_TGCA'G
Cuts at: 830 2217 2744 830
Size: 1387 527 3478
Fragments arranged by size:
3478 1387 527
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5392
PvuII CAG'CTG
Cuts at: 2200 2200
Size: 5392
SalI G'TCGA_C
Cuts at: 1936 2734 1936
Size: 798 4594
SbfI CC_TGCA'GG
Cuts at: 2744 2744
Size: 5392
ScaI AGT'ACT
Cuts at: 2395 3640 5352 2395
Size: 1245 1712 2435
Fragments arranged by size:
2435 1712 1245
SfcI C'TryA_G
Cuts at: 826 1045 2213 2740 3875 4556 4747 826
Size: 219 1168 527 1135 681 191 1471
Fragments arranged by size:
1471 1168 1135 681 527 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5392
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5392
SmaI CCC'GGG
Cuts at: 1872 2054 2719 1872
Size: 182 665 4545
Fragments arranged by size:
4545 665 182
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2476 3496 4397 4659 820
Size: 197 926 533 1020 901 262 1553
Fragments arranged by size:
1553 1020 926 901 533 262 197
SnaBI TAC'GTA
Cuts at: 493 3183 493
Size: 2690 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5392
SphI G_CATG'C
Cuts at: 1132 2750 1132
Size: 1618 3774
SspI AAT'ATT
Cuts at: 5 52 3316 5
Size: 47 3264 2081
Fragments arranged by size:
3264 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5392
StyI C'CwwG_G
Cuts at: 513 1065 2004 2112 2831 513
Size: 552 939 108 719 3074
Fragments arranged by size:
3074 939 719 552 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3419 3436 3589 3774 4219 325
Size: 1255 1839 17 153 185 445 1498
Fragments arranged by size:
1839 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2393 3638 5350
Size: 274 80 33 51 1443 416 1245 1712
Cuts at: 5350 96
Size: 138
Fragments arranged by size:
1712 1443 1245 416 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5352 5384 5352
Size: 32 5360
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5392
XbaI T'CTAG_A
Cuts at: 2728 2728
Size: 5392
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2506 2506
Size: 5392
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5392
XmaI C'CCGG_G
Cuts at: 1870 2052 2717 1870
Size: 182 665 4545
Fragments arranged by size:
4545 665 182
XmnI GAAnn'nnTTC
Cuts at: 3521 3521
Size: 5392
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3200 276
Size: 53 83 186 936 1666 2468
Fragments arranged by size:
2468 1666 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BbeI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI
FseI KasI NarI NruI PacI PfoI PmlI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI