Restriction map for
pFN21AE9684
(Circular) (Six-base) MAPSORT of: pFN21AE9684.seq Check: 9543 from: 1 to: 7219
pFN21AE9684 7219 bp
With 182 enzymes: SgfI *
December 8, 2014 13:55 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3975 3975
Size: 7219
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3787 5029 278
Size: 53 83 186 936 2251 1242 2468
Fragments arranged by size:
2468 2251 1242 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3721 4562 1937
Size: 1784 841 4594
Fragments arranged by size:
4594 1784 841
Acc65I G'GTAC_C
Cuts at: 4293 4540 4293
Size: 247 6972
AclI AA'CG_TT
Cuts at: 1563 5346 5719 1563
Size: 3783 373 3063
Fragments arranged by size:
3783 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3562 4262 4325 5282 6666 6934 1632
Size: 1930 700 63 957 1384 268 1917
Fragments arranged by size:
1930 1917 1384 957 700 268 63
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7022
AflIII A'CryG_T
Cuts at: 1203 6118 7095 1203
Size: 4915 977 1327
Fragments arranged by size:
4915 1327 977
AgeI A'CCGG_T
Cuts at: 6050 6050
Size: 7219
AhdI GACnn_n'nnGTC
Cuts at: 2952 3271 5948 2952
Size: 319 2677 4223
Fragments arranged by size:
4223 2677 319
AleI CACnn'nnGTG
Cuts at: 1166 2849 3422 1166
Size: 1683 573 4963
Fragments arranged by size:
4963 1683 573
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2867 1184
Size: 1683 5536
AlwNI CAG_nnn'CTG
Cuts at: 2806 3335 3394 4432 4485 4962 6534 2806
Size: 529 59 1038 53 477 1572 3491
Fragments arranged by size:
3491 1572 1038 529 477 59 53
ApaI G_GGCC'C
Cuts at: 3881 3881
Size: 7219
ApaLI G'TGCA_C
Cuts at: 2883 5276 6432 2883
Size: 2393 1156 3670
Fragments arranged by size:
3670 2393 1156
ApoI r'AATT_y
Cuts at: 1493 2352 3020 4528 4754 4906 7015 1493
Size: 859 668 1508 226 152 2109 1697
Fragments arranged by size:
2109 1697 1508 859 668 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5773 160
Size: 872 4741 1606
Fragments arranged by size:
4741 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7219
AvaI C'yCGr_G
Cuts at: 1870 1943 2995 3201 4544 1870
Size: 73 1052 206 1343 4545
Fragments arranged by size:
4545 1343 1052 206 73
BamHI G'GATC_C
Cuts at: 2191 4549 4916 2191
Size: 2358 367 4494
Fragments arranged by size:
4494 2358 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2444 2843 3038 3055 4293
Size: 325 217 627 657 399 195 17 1238
Cuts at: 4293 4540 5995 618
Size: 247 1455 1842
Fragments arranged by size:
1842 1455 1238 657 627 399 325 247
217 195 17
BanII G_rGCy'C
Cuts at: 1391 2486 3209 3881 3999 4538 1391
Size: 1095 723 672 118 539 4072
Fragments arranged by size:
4072 1095 723 672 539 118
BbeI G_GCGC'C
Cuts at: 3042 3042
Size: 7219
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2619 4445 4460 928
Size: 971 720 1826 15 3687
Fragments arranged by size:
3687 1826 971 720 15
BbvCI CC'TCA_GC
Cuts at: 3406 3591 3977 3406
Size: 185 386 6648
Fragments arranged by size:
6648 386 185
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5410 5444 1903
Size: 34 3473 34 3678
Fragments arranged by size:
3678 3473 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5111 6321 5111
Size: 1210 6009
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7219
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4307 4394 5830
Size: 107 122 71 631 605 2635 87 1436
Cuts at: 5830 136
Size: 1525
Fragments arranged by size:
2635 1525 1436 631 605 122 107 87
71
BglII A'GATC_T
Cuts at: 4144 7214 4144
Size: 3070 4149
BlpI GC'TnA_GC
Cuts at: 4636 4636
Size: 7219
Bme1580I G_kGCm'C
Cuts at: 1790 2449 2846 2887 3058 3531 3732 3881 5280
Size: 659 397 41 171 473 201 149 1399
Cuts at: 5280 6436 1790
Size: 1156 2573
Fragments arranged by size:
2573 1399 1156 659 473 397 201 171
149 41
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7219
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3083 3232 4028
Size: 416 53 83 399 24 1666 149 796
Cuts at: 4028 4950 5908 442
Size: 922 958 1753
Fragments arranged by size:
1753 1666 958 922 796 416 399 149
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7219
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3793 3793
Size: 7219
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2410 2499 3647 3899 4376 4454 5879 1239
Size: 1171 89 1148 252 477 78 1425 2579
Fragments arranged by size:
2579 1425 1171 1148 477 252 89 78
Bpu10I CC'TnA_GC
Cuts at: 2538 3406 3591 3977 2538
Size: 868 185 386 5780
Fragments arranged by size:
5780 868 386 185
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2258 2452 3077 3124 3671 3685 5344 6209 6507
Size: 194 625 47 547 14 1659 865 298
Cuts at: 6507 2258
Size: 2970
Fragments arranged by size:
2970 1659 865 625 547 298 194 47
14
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2597 3305 3437 3576 3737 5882 882
Size: 617 1098 708 132 139 161 2145 2219
Fragments arranged by size:
2219 2145 1098 708 617 161 139 132
BsaAI yAC'GTr
Cuts at: 493 1204 5010 493
Size: 711 3806 2702
Fragments arranged by size:
3806 2702 711
BsaBI GATnn'nnATC
Cuts at: 4421 7213 4421
Size: 2792 4427
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3039 3784 5026 5408
Size: 53 83 186 936 1506 745 1242 382
Cuts at: 5408 6043 275
Size: 635 1451
Fragments arranged by size:
1506 1451 1242 936 745 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2093 3893 4359 5652 6050 6324 6471 6882 2093
Size: 1800 466 1293 398 274 147 411 2430
Fragments arranged by size:
2430 1800 1293 466 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2740 2770 2881 2911 3973
Size: 30 165 30 1337 30 111 30 1062
Cuts at: 3973 4003 4484 4514 1178
Size: 30 481 30 3883
Fragments arranged by size:
3883 1337 1062 481 165 111 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2717 2802 2983 3013 3164 3380 3584 3977
Size: 1531 85 181 30 151 216 204 393
Cuts at: 3977 3994 4007 4334 4358 4484 1186
Size: 17 13 327 24 126 3921
Fragments arranged by size:
3921 1531 393 327 216 204 181 151
126 85 30 24 17 13
BseYI C'CCAG_C
Cuts at: 2404 2634 3291 3319 3350 6422 6918 2404
Size: 230 657 28 31 3072 496 2705
Fragments arranged by size:
3072 2705 657 496 230 31 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3449 1163
Size: 573 1713 4933
Fragments arranged by size:
4933 1713 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4669 5430 6458 1540
Size: 275 186 2668 761 1028 2301
Fragments arranged by size:
2668 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2734 2794 2887 2909 3829 3999 5280 5365 6436
Size: 60 93 22 920 170 1281 85 1071
Cuts at: 6436 2734
Size: 3517
Fragments arranged by size:
3517 1281 1071 920 170 93 85 60
22
BsmI GAATG_Cn'
Cuts at: 2678 2714 3397 4730 4823 2678
Size: 36 683 1333 93 5074
Fragments arranged by size:
5074 1333 683 93 36
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2449 2486 2734 2794 2846 2887 2909
Size: 399 659 37 248 60 52 41 22
Cuts at: 2909 3058 3209 3531 3732 3829 3881 3999 4538
Size: 149 151 322 201 97 52 118 539
Cuts at: 4538 5280 5365 6436 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 659 539 399 322 248
201 151 149 118 97 85 60 52
52 41 37 22
BspEI T'CCGG_A
Cuts at: 4359 6882 4359
Size: 2523 4696
BspHI T'CATG_A
Cuts at: 5108 6838 5108
Size: 1730 5489
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2005 3918 3975 4574 844
Size: 1161 1913 57 599 3489
Fragments arranged by size:
3489 1913 1161 599 57
BsrBI CCG'CTC
Cuts at: 1394 1823 3003 3168 3173 5106 1394
Size: 429 1180 165 5 1933 3507
Fragments arranged by size:
3507 1933 1180 429 165 5
BsrDI GCAATG_nn'
Cuts at: 65 1248 2047 5714 5888 65
Size: 1183 799 3667 174 1396
Fragments arranged by size:
3667 1396 1183 799 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2712 5863 6050 1700
Size: 110 902 3151 187 2869
Fragments arranged by size:
3151 2869 902 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7219
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7219
BssSI C'ACGA_G
Cuts at: 5279 6291 5279
Size: 1012 6207
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2806 794
Size: 2012 5207
BstBI TT'CG_AA
Cuts at: 4041 6023 4041
Size: 1982 5237
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7219
BstXI CCAn_nnnn'nTGG
Cuts at: 2149 2926 3805 2149
Size: 777 879 5563
Fragments arranged by size:
5563 879 777
BstYI r'GATC_y
Cuts at: 1972 2191 4144 4482 4549 4916 5301 5318 6770
Size: 219 1953 338 67 367 385 17 1452
Cuts at: 6770 6856 6868 7214 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1953 1452 385 367 346 338 219
86 67 17 12
Bsu36I CC'TnA_GG
Cuts at: 2772 2958 3704 3778 4403 2772
Size: 186 746 74 625 5588
Fragments arranged by size:
5588 746 625 186 74
BtgI C'CryG_G
Cuts at: 513 1065 2547 3360 3732 3788 4000 4062 4155
Size: 552 1482 813 372 56 212 62 93
Cuts at: 4155 513
Size: 3577
Fragments arranged by size:
3577 1482 813 552 372 212 93 62
56
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6480
Fragments arranged by size:
6480 683 56
BtsI GCAGTG_nn'
Cuts at: 2748 3123 3289 3523 3948 4735 5529 5549 2748
Size: 375 166 234 425 787 794 20 4418
Fragments arranged by size:
4418 794 787 425 375 234 166 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7219
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7184
DraI TTT'AAA
Cuts at: 4523 4523
Size: 7219
DraIII CAC_nnn'GTG
Cuts at: 2929 2929
Size: 7219
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2033 6226 809
Size: 507 717 4193 1802
Fragments arranged by size:
4193 1802 717 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2151 2226 2745
Size: 54 1425 50 275 18 321 75 519
Cuts at: 2745 2985 3735 4666 5555 7184 8
Size: 240 750 931 889 1629 43
Fragments arranged by size:
1629 1425 931 889 750 519 321 275
240 75 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4666 1537
Size: 275 2854 4090
Fragments arranged by size:
4090 2854 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2479 2578 4038 4184 4227 5149 1353
Size: 1126 99 1460 146 43 922 3423
Fragments arranged by size:
3423 1460 1126 922 146 99 43
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4391 5806 6180 6326 387
Size: 1064 138 2802 1415 374 146 1280
Fragments arranged by size:
2802 1415 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3997 3997
Size: 7219
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2410 2499 3562 3647 3899 4262 4325
Size: 393 778 89 1063 85 252 363 63
Cuts at: 4325 4376 4454 5282 5879 6666 6934 1239
Size: 51 78 828 597 787 268 1524
Fragments arranged by size:
1524 1063 828 787 778 597 393 363
268 252 89 85 78 63 51
EcoNI CCTnn'n_nnAGG
Cuts at: 3843 4406 3843
Size: 563 6656
EcoO109I rG'GnC_Cy
Cuts at: 3133 3181 3374 3877 3931 3133
Size: 48 193 503 54 6421
Fragments arranged by size:
6421 503 193 54 48
EcoRI G'AATT_C
Cuts at: 2352 4528 4906 2352
Size: 2176 378 4665
Fragments arranged by size:
4665 2176 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3118 3694 3818 4151 3118
Size: 576 124 333 6186
Fragments arranged by size:
6186 576 333 124
FspI TGC'GCA
Cuts at: 4421 5725 4421
Size: 1304 5915
FspAI rTGC'GCAy
Cuts at: 4421 4421
Size: 7219
HaeII r_GCGC'y
Cuts at: 1678 3042 6366 1678
Size: 1364 3324 2531
Fragments arranged by size:
3324 2531 1364
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2200 2232 2938 2970 3257
Size: 32 135 32 628 32 706 32 287
Cuts at: 3257 3289 3697 3729 4136 4168 4484 4516 5860
Size: 32 408 32 407 32 316 32 1344
Cuts at: 5860 5892 5934 5966 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1344 706 628 408 407 316 287
135 42 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4563 4809 669
Size: 988 281 2625 246 3079
Fragments arranged by size:
3079 2625 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3800 748
Size: 3052 4167
HpaI GTT'AAC
Cuts at: 4809 4809
Size: 7219
KasI G'GCGC_C
Cuts at: 3038 3038
Size: 7219
KpnI G_GTAC'C
Cuts at: 4297 4544 4297
Size: 247 6972
MfeI C'AATT_G
Cuts at: 4818 4818
Size: 7219
MluI A'CGCG_T
Cuts at: 7095 7095
Size: 7219
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4197 6333 6517 1305
Size: 2892 2136 184 2007
Fragments arranged by size:
2892 2136 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2153 2228 2747 2987 3737
Size: 54 1425 343 321 75 519 240 750
Cuts at: 3737 10
Size: 3492
Fragments arranged by size:
3492 1425 750 519 343 321 240 75
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2849 3422 3839 5177 5536 5695 518
Size: 648 1683 573 417 1338 359 159 2042
Fragments arranged by size:
2042 1683 1338 648 573 417 359 159
MspA1I CmG'CkG
Cuts at: 1606 3332 3394 3410 3590 3790 4333 4635 5312
Size: 1726 62 16 180 200 543 302 677
Cuts at: 5312 6460 6705 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1726 1148 677 543 302 245 200
180 62 16
NaeI GCC'GGC
Cuts at: 2714 2714
Size: 7219
NarI GG'CG_CC
Cuts at: 3039 3039
Size: 7219
NcoI C'CATG_G
Cuts at: 513 1065 4062 4155 513
Size: 552 2997 93 3577
Fragments arranged by size:
3577 2997 552 93
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7219
NgoMIV G'CCGG_C
Cuts at: 2712 2712
Size: 7219
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7219
NotI GC'GGCC_GC
Cuts at: 4666 4666
Size: 7219
NspI r_CATG'y
Cuts at: 1132 1229 3350 3757 4577 6122 1132
Size: 97 2121 407 820 1545 2229
Fragments arranged by size:
2229 2121 1545 820 407 97
PasI CC'CwG_GG
Cuts at: 1348 2821 1348
Size: 1473 5746
PciI A'CATG_T
Cuts at: 6118 6118
Size: 7219
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2411 2929 4063 4432 1108
Size: 1303 518 1134 369 3895
Fragments arranged by size:
3895 1303 1134 518 369
PfoI T'CCnGG_A
Cuts at: 2515 2515
Size: 7219
PmeI GTTT'AAAC
Cuts at: 4523 4523
Size: 7219
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5288 5320 6801 6833 5288
Size: 32 1481 32 5674
Fragments arranged by size:
5674 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3181 3374 3931 3181
Size: 193 557 6469
Fragments arranged by size:
6469 557 193
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7219
PsiI TTA'TAA
Cuts at: 4789 4789
Size: 7219
PspOMI G'GGCC_C
Cuts at: 3877 3877
Size: 7219
PspXI vC'TCGA_Gb
Cuts at: 2995 2995
Size: 7219
PstI C_TGCA'G
Cuts at: 830 2016 2802 2977 4332 4571 830
Size: 1186 786 175 1355 239 3478
Fragments arranged by size:
3478 1355 1186 786 239 175
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7219
PvuII CAG'CTG
Cuts at: 3394 4333 3394
Size: 939 6280
SacI G_AGCT'C
Cuts at: 3999 3999
Size: 7219
SacII CC_GC'GG
Cuts at: 3791 3791
Size: 7219
SalI G'TCGA_C
Cuts at: 1936 4561 1936
Size: 2625 4594
SapI GCTCTTCn'nnn_
Cuts at: 2479 2479
Size: 7219
SbfI CC_TGCA'GG
Cuts at: 2802 4571 2802
Size: 1769 5450
ScaI AGT'ACT
Cuts at: 5467 7179 5467
Size: 1712 5507
SexAI A'CCwGG_T
Cuts at: 2925 2925
Size: 7219
SfcI C'TryA_G
Cuts at: 826 1045 2012 2357 2798 2973 3900 4328 4567
Size: 219 967 345 441 175 927 428 239
Cuts at: 4567 5702 6383 6574 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1135 967 927 681 441 428 345
239 219 191 175
SfiI GGCCn_nnn'nGGCC
Cuts at: 4394 4394
Size: 7219
SfoI GGC'GCC
Cuts at: 3040 3040
Size: 7219
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7219
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7219
SmaI CCC'GGG
Cuts at: 1872 4546 1872
Size: 2674 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2237 2467 2995 3092 3103 3664
Size: 197 926 294 230 528 97 11 561
Cuts at: 3664 3686 5323 6224 6486 820
Size: 22 1637 901 262 1553
Fragments arranged by size:
1637 1553 926 901 561 528 294 262
230 197 97 22 11
SnaBI TAC'GTA
Cuts at: 493 5010 493
Size: 4517 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7219
SphI G_CATG'C
Cuts at: 1132 3350 3757 4577 1132
Size: 2218 407 820 3774
Fragments arranged by size:
3774 2218 820 407
SspI AAT'ATT
Cuts at: 5 52 5143 5
Size: 47 5091 2081
Fragments arranged by size:
5091 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7219
StyI C'CwwG_G
Cuts at: 513 1065 2266 3065 3555 4062 4155 4658 513
Size: 552 1201 799 490 507 93 503 3074
Fragments arranged by size:
3074 1201 799 552 507 503 490 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2155 3548 4292 5246 5263 5416 5601
Size: 1255 575 1393 744 954 17 153 185
Cuts at: 5601 6046 325
Size: 445 1498
Fragments arranged by size:
1498 1393 1255 954 744 575 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3918 5465 7177
Size: 274 80 33 51 1443 1941 1547 1712
Cuts at: 7177 96
Size: 138
Fragments arranged by size:
1941 1712 1547 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2740 2772 2881 2913 3481 3493 3513 3525 3888
Size: 32 109 32 568 12 20 12 363
Cuts at: 3888 3920 3973 4005 7179 7211 2740
Size: 32 53 32 3174 32 2748
Fragments arranged by size:
3174 2748 568 363 109 53 32 32
32 32 32 20 12 12
Tth111I GACn'n_nGTC
Cuts at: 1316 2629 1316
Size: 1313 5906
XbaI T'CTAG_A
Cuts at: 4555 4555
Size: 7219
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2926 2930 2926
Size: 4 7215
XhoI C'TCGA_G
Cuts at: 1943 2995 1943
Size: 1052 6167
XmaI C'CCGG_G
Cuts at: 1870 4544 1870
Size: 2674 4545
XmnI GAAnn'nnTTC
Cuts at: 4166 5348 4166
Size: 1182 6037
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3785 5027 276
Size: 53 83 186 936 2251 1242 2468
Fragments arranged by size:
2468 2251 1242 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI
PvuI PvuII SacI SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstZ17I EcoRV FseI NruI NsiI PacI PmlI PsrI
RsrII SanDI SrfI SwaI