(Circular) (Six-base) MAPSORT of: pFN21AE9681.seq Check: 2956 from: 1 to: 5068 pFN21AE9681 5068 bp With 182 enzymes: SgfI * December 8, 2014 13:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2878 278 Size: 53 83 186 936 1342 2468 Fragments arranged by size: 2468 1342 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2411 1937 Size: 474 4594 Acc65I G'GTAC_C Cuts at: 2389 2389 Size: 5068 AclI AA'CG_TT Cuts at: 1563 3195 3568 1563 Size: 1632 373 3063 Fragments arranged by size: 3063 1632 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3131 4515 4783 1632 Size: 1499 1384 268 1917 Fragments arranged by size: 1917 1499 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4871 AflIII A'CryG_T Cuts at: 1203 3967 4944 1203 Size: 2764 977 1327 Fragments arranged by size: 2764 1327 977 AgeI A'CCGG_T Cuts at: 3899 3899 Size: 5068 AhdI GACnn_n'nnGTC Cuts at: 3797 3797 Size: 5068 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5068 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5068 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2021 2022 2053 2054 2021 Size: 1 31 1 5035 Fragments arranged by size: 5035 31 1 1 AlwNI CAG_nnn'CTG Cuts at: 2207 2811 4383 2207 Size: 604 1572 2892 Fragments arranged by size: 2892 1572 604 ApaLI G'TGCA_C Cuts at: 3125 4281 3125 Size: 1156 3912 ApoI r'AATT_y Cuts at: 1493 2348 2377 2603 2755 4864 1493 Size: 855 29 226 152 2109 1697 Fragments arranged by size: 2109 1697 855 226 152 29 AseI AT'TA_AT Cuts at: 160 1032 3622 160 Size: 872 2590 1606 Fragments arranged by size: 2590 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5068 AvaI C'yCGr_G Cuts at: 1870 1943 2393 1870 Size: 73 450 4545 Fragments arranged by size: 4545 450 73 BamHI G'GATC_C Cuts at: 2398 2765 2398 Size: 367 4701 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2389 3844 618 Size: 325 217 627 602 1455 1842 Fragments arranged by size: 1842 1455 627 602 325 217 BanII G_rGCy'C Cuts at: 1391 2387 1391 Size: 996 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4097 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3259 3293 1903 Size: 34 1322 34 3678 Fragments arranged by size: 3678 1322 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2960 4170 2960 Size: 1210 3858 BclI T'GATC_A Cuts at: 1548 1548 Size: 5068 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3679 136 Size: 107 122 71 631 605 337 1670 1525 Fragments arranged by size: 1670 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5063 5063 Size: 5068 BlpI GC'TnA_GC Cuts at: 2485 2485 Size: 5068 Bme1580I G_kGCm'C Cuts at: 1790 3129 4285 1790 Size: 1339 1156 2573 Fragments arranged by size: 2573 1339 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5068 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2799 3757 442 Size: 416 53 83 399 24 1382 958 1753 Fragments arranged by size: 1753 1382 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5068 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2318 3728 1239 Size: 1079 1410 2579 Fragments arranged by size: 2579 1410 1079 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3193 4058 4356 3193 Size: 865 298 3905 Fragments arranged by size: 3905 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3731 882 Size: 617 2232 2219 Fragments arranged by size: 2232 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2859 493 Size: 711 1655 2702 Fragments arranged by size: 2702 1655 711 BsaBI GATnn'nnATC Cuts at: 5062 5062 Size: 5068 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2875 3257 3892 275 Size: 53 83 186 936 1342 382 635 1451 Fragments arranged by size: 1451 1342 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3501 3899 4173 4320 4731 3501 Size: 398 274 147 411 3838 Fragments arranged by size: 3838 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4843 Fragments arranged by size: 4843 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5068 BseYI C'CCAG_C Cuts at: 4271 4767 4271 Size: 496 4572 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4495 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2518 3279 4307 1540 Size: 275 186 517 761 1028 2301 Fragments arranged by size: 2301 1028 761 517 275 186 BsiHKAI G_wGCw'C Cuts at: 3129 3214 4285 3129 Size: 85 1071 3912 Fragments arranged by size: 3912 1071 85 BsmI GAATG_Cn' Cuts at: 2579 2672 2579 Size: 93 4975 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2387 3129 3214 4285 1391 Size: 399 597 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 597 399 85 BspEI T'CCGG_A Cuts at: 4731 4731 Size: 5068 BspHI T'CATG_A Cuts at: 2957 4687 2957 Size: 1730 3338 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2423 844 Size: 1579 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2955 1394 Size: 429 1132 3507 Fragments arranged by size: 3507 1132 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3563 3737 65 Size: 1183 2315 174 1396 Fragments arranged by size: 2315 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3712 3899 1700 Size: 110 1902 187 2869 Fragments arranged by size: 2869 1902 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5068 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5068 BssSI C'ACGA_G Cuts at: 3128 4140 3128 Size: 1012 4056 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5068 BstBI TT'CG_AA Cuts at: 3872 3872 Size: 5068 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5068 BstYI r'GATC_y Cuts at: 1972 2398 2765 3150 3167 4619 4705 4717 5063 Size: 426 367 385 17 1452 86 12 346 Cuts at: 5063 1972 Size: 1977 Fragments arranged by size: 1977 1452 426 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3577 Fragments arranged by size: 3577 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4329 Fragments arranged by size: 4329 683 56 BtsI GCAGTG_nn' Cuts at: 2584 3378 3398 2584 Size: 794 20 4254 Fragments arranged by size: 4254 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5068 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5033 DraI TTT'AAA Cuts at: 2197 2372 2197 Size: 175 4893 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4075 809 Size: 507 2759 1802 Fragments arranged by size: 2759 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2515 3404 5033 Size: 54 1425 50 275 18 685 889 1629 Cuts at: 5033 8 Size: 43 Fragments arranged by size: 1629 1425 889 685 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2515 1537 Size: 275 703 4090 Fragments arranged by size: 4090 703 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2316 2998 1353 Size: 963 682 3423 Fragments arranged by size: 3423 963 682 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2027 3655 4029 4175 387 Size: 1064 138 438 1628 374 146 1280 Fragments arranged by size: 1628 1280 1064 438 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2318 3131 3728 4515 4783 1239 Size: 393 686 813 597 787 268 1524 Fragments arranged by size: 1524 813 787 686 597 393 268 EcoRI G'AATT_C Cuts at: 2377 2755 2377 Size: 378 4690 EcoRV GAT'ATC Cuts at: 2174 2174 Size: 5068 FspI TGC'GCA Cuts at: 3574 3574 Size: 5068 HaeII r_GCGC'y Cuts at: 1678 4215 1678 Size: 2537 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2089 2121 2152 2184 3709 Size: 32 135 32 517 32 31 32 1525 Cuts at: 3709 3741 3783 3815 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1525 517 135 42 32 32 32 32 32 32 31 HincII GTy'rAC Cuts at: 669 1657 1938 2412 2658 669 Size: 988 281 474 246 3079 Fragments arranged by size: 3079 988 474 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5068 HpaI GTT'AAC Cuts at: 2658 2658 Size: 5068 KpnI G_GTAC'C Cuts at: 2393 2393 Size: 5068 MfeI C'AATT_G Cuts at: 2667 2667 Size: 5068 MluI A'CGCG_T Cuts at: 4944 4944 Size: 5068 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4182 4366 1305 Size: 2877 184 2007 Fragments arranged by size: 2877 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3246 Fragments arranged by size: 3246 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3026 3385 3544 518 Size: 648 1860 359 159 2042 Fragments arranged by size: 2042 1860 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2484 3161 4309 4554 1606 Size: 878 677 1148 245 2120 Fragments arranged by size: 2120 1148 878 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3577 Fragments arranged by size: 3577 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5068 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5068 NotI GC'GGCC_GC Cuts at: 2515 2515 Size: 5068 NspI r_CATG'y Cuts at: 1132 1229 2359 2426 3971 1132 Size: 97 1130 67 1545 2229 Fragments arranged by size: 2229 1545 1130 97 67 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5068 PciI A'CATG_T Cuts at: 3967 3967 Size: 5068 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5068 PmeI GTTT'AAAC Cuts at: 2372 2372 Size: 5068 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3137 3169 4650 4682 3137 Size: 32 1481 32 3523 Fragments arranged by size: 3523 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5068 PsiI TTA'TAA Cuts at: 2638 2638 Size: 5068 PstI C_TGCA'G Cuts at: 830 2420 830 Size: 1590 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5068 SalI G'TCGA_C Cuts at: 1936 2410 1936 Size: 474 4594 SbfI CC_TGCA'GG Cuts at: 2420 2420 Size: 5068 ScaI AGT'ACT Cuts at: 3316 5028 3316 Size: 1712 3356 SfcI C'TryA_G Cuts at: 826 1045 2279 2416 3551 4232 4423 826 Size: 219 1234 137 1135 681 191 1471 Fragments arranged by size: 1471 1234 1135 681 219 191 137 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5068 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5068 SmaI CCC'GGG Cuts at: 1872 2395 1872 Size: 523 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3172 4073 4335 820 Size: 197 926 1229 901 262 1553 Fragments arranged by size: 1553 1229 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2859 493 Size: 2366 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5068 SphI G_CATG'C Cuts at: 1132 2426 1132 Size: 1294 3774 SspI AAT'ATT Cuts at: 5 52 2992 5 Size: 47 2940 2081 Fragments arranged by size: 2940 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5068 StyI C'CwwG_G Cuts at: 513 1065 2004 2507 513 Size: 552 939 503 3074 Fragments arranged by size: 3074 939 552 503 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3095 3112 3265 3450 3895 325 Size: 1255 1515 17 153 185 445 1498 Fragments arranged by size: 1515 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3314 5026 96 Size: 274 80 33 51 1443 1337 1712 138 Fragments arranged by size: 1712 1443 1337 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5028 5060 5028 Size: 32 5036 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5068 XbaI T'CTAG_A Cuts at: 2404 2404 Size: 5068 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5068 XmaI C'CCGG_G Cuts at: 1870 2393 1870 Size: 523 4545 XmnI GAAnn'nnTTC Cuts at: 3197 3197 Size: 5068 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2876 276 Size: 53 83 186 936 1342 2468 Fragments arranged by size: 2468 1342 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI