Restriction map for
pFN21AE9680
(Circular) (Six-base) MAPSORT of: pFN21AE9680.seq Check: 7421 from: 1 to: 7126
pFN21AE9680 7126 bp
With 182 enzymes: SgfI *
December 8, 2014 13:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4936 278
Size: 53 83 186 936 3400 2468
Fragments arranged by size:
3400 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3574 4469 1937
Size: 1637 895 4594
Fragments arranged by size:
4594 1637 895
Acc65I G'GTAC_C
Cuts at: 4447 4447
Size: 7126
AclI AA'CG_TT
Cuts at: 1563 5253 5626 1563
Size: 3690 373 3063
Fragments arranged by size:
3690 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3791 4002 4239 5189 6573 6841 1632
Size: 2159 211 237 950 1384 268 1917
Fragments arranged by size:
2159 1917 1384 950 268 237 211
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6929
AflIII A'CryG_T
Cuts at: 1203 6025 7002 1203
Size: 4822 977 1327
Fragments arranged by size:
4822 1327 977
AgeI A'CCGG_T
Cuts at: 5957 5957
Size: 7126
AhdI GACnn_n'nnGTC
Cuts at: 3883 5855 3883
Size: 1972 5154
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7126
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2093 3813 1184
Size: 909 1720 4497
Fragments arranged by size:
4497 1720 909
AlwNI CAG_nnn'CTG
Cuts at: 3686 4295 4869 6441 3686
Size: 609 574 1572 4371
Fragments arranged by size:
4371 1572 609 574
ApaLI G'TGCA_C
Cuts at: 3939 4141 4196 5183 6339 3939
Size: 202 55 987 1156 4726
Fragments arranged by size:
4726 1156 987 202 55
ApoI r'AATT_y
Cuts at: 1493 2723 3134 3268 3604 4435 4661 4813 6922
Size: 1230 411 134 336 831 226 152 2109
Cuts at: 6922 1493
Size: 1697
Fragments arranged by size:
2109 1697 1230 831 411 336 226 152
134
AseI AT'TA_AT
Cuts at: 160 1032 5680 160
Size: 872 4648 1606
Fragments arranged by size:
4648 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7126
AvaI C'yCGr_G
Cuts at: 1870 1943 4451 1870
Size: 73 2508 4545
Fragments arranged by size:
4545 2508 73
BamHI G'GATC_C
Cuts at: 4456 4823 4456
Size: 367 6759
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4447 5902 618
Size: 325 217 627 2660 1455 1842
Fragments arranged by size:
2660 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3866 4445 1391
Size: 2475 579 4072
Fragments arranged by size:
4072 2475 579
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 6155
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5317 5351 1903
Size: 34 3380 34 3678
Fragments arranged by size:
3678 3380 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5018 6228 5018
Size: 1210 5916
BclI T'GATC_A
Cuts at: 1548 3081 1548
Size: 1533 5593
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5737 136
Size: 107 122 71 631 605 4065 1525
Fragments arranged by size:
4065 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7121 7121
Size: 7126
BlpI GC'TnA_GC
Cuts at: 4543 4543
Size: 7126
Bme1580I G_kGCm'C
Cuts at: 1790 2121 3943 4145 4200 5187 6343 1790
Size: 331 1822 202 55 987 1156 2573
Fragments arranged by size:
2573 1822 1156 987 331 202 55
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7126
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2545 3149 3410
Size: 416 53 83 399 24 1128 604 261
Cuts at: 3410 4857 5815 442
Size: 1447 958 1753
Fragments arranged by size:
1753 1447 1128 958 604 416 399 261
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7126
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2883 4209 5786 1239
Size: 1644 1326 1577 2579
Fragments arranged by size:
2579 1644 1577 1326
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3622 3850 5251 6116 6414 3622
Size: 228 1401 865 298 4334
Fragments arranged by size:
4334 1401 865 298 228
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5789 882
Size: 617 4290 2219
Fragments arranged by size:
4290 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4917 493
Size: 711 3713 2702
Fragments arranged by size:
3713 2702 711
BsaBI GATnn'nnATC
Cuts at: 7120 7120
Size: 7126
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2040 4933 5315 5950
Size: 53 83 186 936 507 2893 382 635
Cuts at: 5950 275
Size: 1451
Fragments arranged by size:
2893 1451 936 635 507 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3166 5559 5957 6231 6378 6789 3166
Size: 2393 398 274 147 411 3503
Fragments arranged by size:
3503 2393 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2084 2114 1178
Size: 30 165 30 681 30 6190
Fragments arranged by size:
6190 681 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2162 3225 1186
Size: 976 1063 5087
Fragments arranged by size:
5087 1063 976
BseYI C'CCAG_C
Cuts at: 3801 4132 4328 6329 6825 3801
Size: 331 196 2001 496 4102
Fragments arranged by size:
4102 2001 496 331 196
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6553
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4576 5337 6365 1540
Size: 275 186 2575 761 1028 2301
Fragments arranged by size:
2575 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3943 4145 4200 5187 5272 6343 3943
Size: 202 55 987 85 1071 4726
Fragments arranged by size:
4726 1071 987 202 85 55
BsmI GAATG_Cn'
Cuts at: 3829 4637 4730 3829
Size: 808 93 6225
Fragments arranged by size:
6225 808 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2104 2710 2104
Size: 606 6520
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2121 3866 3943 4145 4200 4445 5187
Size: 399 331 1745 77 202 55 245 742
Cuts at: 5187 5272 6343 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1745 1071 742 399 331 245 202
85 77 55
BspEI T'CCGG_A
Cuts at: 6789 6789
Size: 7126
BspHI T'CATG_A
Cuts at: 5015 6745 5015
Size: 1730 5396
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4481 844
Size: 3637 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5013 1394
Size: 429 3190 3507
Fragments arranged by size:
3507 3190 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3071 3307 5621 5795 65
Size: 1183 1823 236 2314 174 1396
Fragments arranged by size:
2314 1823 1396 1183 236 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5770 5957 1700
Size: 110 3960 187 2869
Fragments arranged by size:
3960 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2271 4084 96
Size: 2175 1813 3138
Fragments arranged by size:
3138 2175 1813
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7126
BssSI C'ACGA_G
Cuts at: 5186 6198 5186
Size: 1012 6114
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7126
BstBI TT'CG_AA
Cuts at: 5930 5930
Size: 7126
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7126
BstXI CCAn_nnnn'nTGG
Cuts at: 2137 4051 2137
Size: 1914 5212
BstYI r'GATC_y
Cuts at: 1972 4456 4823 5208 5225 6677 6763 6775 7121
Size: 2484 367 385 17 1452 86 12 346
Cuts at: 7121 1972
Size: 1977
Fragments arranged by size:
2484 1977 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2235 2235
Size: 7126
BtgI C'CryG_G
Cuts at: 513 1065 2065 513
Size: 552 1000 5574
Fragments arranged by size:
5574 1000 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4248 487
Size: 683 56 3022 3365
Fragments arranged by size:
3365 3022 683 56
BtsI GCAGTG_nn'
Cuts at: 3936 3964 4200 4339 4642 5436 5456 3936
Size: 28 236 139 303 794 20 5606
Fragments arranged by size:
5606 794 303 236 139 28 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7126
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3129 3164 529
Size: 35 2565 35 4491
Fragments arranged by size:
4491 2565 35 35
DraI TTT'AAA
Cuts at: 4430 4430
Size: 7126
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6133 809
Size: 507 4817 1802
Fragments arranged by size:
4817 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3985 4105 4573
Size: 54 1425 50 275 18 2155 120 468
Cuts at: 4573 5462 7091 8
Size: 889 1629 43
Fragments arranged by size:
2155 1629 1425 889 468 275 120 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4573 1537
Size: 275 2761 4090
Fragments arranged by size:
4090 2761 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2758 2913 2973 3469 3738 5056 1353
Size: 1405 155 60 496 269 1318 3423
Fragments arranged by size:
3423 1405 1318 496 269 155 60
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5713 6087 6233 387
Size: 1064 138 4124 374 146 1280
Fragments arranged by size:
4124 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2883 3791 4002 4209 4239 5189 5786
Size: 393 1251 908 211 207 30 950 597
Cuts at: 5786 6573 6841 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1251 950 908 787 597 393 268
211 207 30
EcoRI G'AATT_C
Cuts at: 4435 4813 4435
Size: 378 6748
FspI TGC'GCA
Cuts at: 5632 5632
Size: 7126
HaeII r_GCGC'y
Cuts at: 1678 2036 3912 6273 1678
Size: 358 1876 2361 2531
Fragments arranged by size:
2531 2361 1876 358
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2084 2116 2138 2170 2675
Size: 32 135 32 512 32 22 32 505
Cuts at: 2675 2707 3034 3066 3383 3415 3869 3901 5767
Size: 32 327 32 317 32 454 32 1866
Cuts at: 5767 5799 5841 5873 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1866 512 505 454 327 317 135
42 32 32 32 32 32 32 32
32 32 32 22
HincII GTy'rAC
Cuts at: 669 1657 1938 2254 3575 4470 4716 669
Size: 988 281 316 1321 895 246 3079
Fragments arranged by size:
3079 1321 988 895 316 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7126
HpaI GTT'AAC
Cuts at: 4716 4716
Size: 7126
KpnI G_GTAC'C
Cuts at: 4451 4451
Size: 7126
MfeI C'AATT_G
Cuts at: 4725 4725
Size: 7126
MluI A'CGCG_T
Cuts at: 7002 7002
Size: 7126
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6240 6424 1305
Size: 4935 184 2007
Fragments arranged by size:
4935 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3987 4107 10
Size: 54 1425 343 2155 120 3029
Fragments arranged by size:
3029 2155 1425 343 120 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2135 3261 4049 5084 5443 5602 518
Size: 648 969 1126 788 1035 359 159 2042
Fragments arranged by size:
2042 1126 1035 969 788 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2053 2067 4082 4542 5219 6367 6612 1606
Size: 447 14 2015 460 677 1148 245 2120
Fragments arranged by size:
2120 2015 1148 677 460 447 245 14
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6574
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7126
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7126
NotI GC'GGCC_GC
Cuts at: 4573 4573
Size: 7126
NspI r_CATG'y
Cuts at: 1132 1229 2080 4484 6029 1132
Size: 97 851 2404 1545 2229
Fragments arranged by size:
2404 2229 1545 851 97
PasI CC'CwG_GG
Cuts at: 1348 4070 1348
Size: 2722 4404
PciI A'CATG_T
Cuts at: 6025 6025
Size: 7126
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 7126
PmeI GTTT'AAAC
Cuts at: 4430 4430
Size: 7126
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5195 5227 6708 6740 5195
Size: 32 1481 32 5581
Fragments arranged by size:
5581 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7126
PsiI TTA'TAA
Cuts at: 4696 4696
Size: 7126
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2151 2183 2151
Size: 32 7094
PstI C_TGCA'G
Cuts at: 830 2561 3682 3931 4478 830
Size: 1731 1121 249 547 3478
Fragments arranged by size:
3478 1731 1121 547 249
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7126
PvuII CAG'CTG
Cuts at: 4082 4082
Size: 7126
SacII CC_GC'GG
Cuts at: 2068 2068
Size: 7126
SalI G'TCGA_C
Cuts at: 1936 3573 4468 1936
Size: 1637 895 4594
Fragments arranged by size:
4594 1637 895
SapI GCTCTTCn'nnn_
Cuts at: 3469 3738 3469
Size: 269 6857
SbfI CC_TGCA'GG
Cuts at: 4478 4478
Size: 7126
ScaI AGT'ACT
Cuts at: 5374 7086 5374
Size: 1712 5414
SfcI C'TryA_G
Cuts at: 826 1045 2557 3474 3578 3678 3687 3927 4474
Size: 219 1512 917 104 100 9 240 547
Cuts at: 4474 5609 6290 6481 826
Size: 1135 681 191 1471
Fragments arranged by size:
1512 1471 1135 917 681 547 240 219
191 104 100 9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7126
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7126
SmaI CCC'GGG
Cuts at: 1872 4453 1872
Size: 2581 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3637 3865 5230 6131 6393 820
Size: 197 926 1694 228 1365 901 262 1553
Fragments arranged by size:
1694 1553 1365 926 901 262 228 197
SnaBI TAC'GTA
Cuts at: 493 4917 493
Size: 4424 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7126
SphI G_CATG'C
Cuts at: 1132 4484 1132
Size: 3352 3774
SspI AAT'ATT
Cuts at: 5 52 3552 5050 5
Size: 47 3500 1498 2081
Fragments arranged by size:
3500 2081 1498 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 7126
StyI C'CwwG_G
Cuts at: 513 1065 3593 3969 4565 513
Size: 552 2528 376 596 3074
Fragments arranged by size:
3074 2528 596 552 376
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2527 3408 5153 5170 5323 5508 5953
Size: 1255 947 881 1745 17 153 185 445
Cuts at: 5953 325
Size: 1498
Fragments arranged by size:
1745 1498 1255 947 881 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2271 4084 5372
Size: 274 80 33 51 1443 294 1813 1288
Cuts at: 5372 7084 96
Size: 1712 138
Fragments arranged by size:
1813 1712 1443 1288 294 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3312 3344 4135 4167 7086 7118 3312
Size: 32 791 32 2919 32 3320
Fragments arranged by size:
3320 2919 791 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2096 3416 1316
Size: 780 1320 5026
Fragments arranged by size:
5026 1320 780
XbaI T'CTAG_A
Cuts at: 2326 4462 2326
Size: 2136 4990
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2743 2743
Size: 7126
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7126
XmaI C'CCGG_G
Cuts at: 1870 4451 1870
Size: 2581 4545
XmnI GAAnn'nnTTC
Cuts at: 5255 5255
Size: 7126
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4934 276
Size: 53 83 186 936 3400 2468
Fragments arranged by size:
3400 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII
SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI RsrII SacI SanDI SexAI SfiI SfoI
SrfI SwaI