Restriction map for
pFN21AE9678
(Circular) (Six-base) MAPSORT of: pFN21AE9678.seq Check: 7316 from: 1 to: 8956
pFN21AE9678 8956 bp
With 182 enzymes: SgfI *
March 28, 2012 12:15 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6766 278
Size: 53 83 186 936 5230 2468
Fragments arranged by size:
5230 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 5349 5565 6299 1937
Size: 3412 216 734 4594
Fragments arranged by size:
4594 3412 734 216
Acc65I G'GTAC_C
Cuts at: 6277 6277
Size: 8956
AclI AA'CG_TT
Cuts at: 1563 7083 7456 1563
Size: 5520 373 3063
Fragments arranged by size:
5520 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2115 2206 2757 3191 4559 5826 5901 7019
Size: 483 91 551 434 1368 1267 75 1118
Cuts at: 7019 8403 8671 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1384 1368 1267 1118 551 483 434
268 91 75
AflII C'TTAA_G
Cuts at: 820 1017 6233 820
Size: 197 5216 3543
Fragments arranged by size:
5216 3543 197
AflIII A'CryG_T
Cuts at: 1203 4722 5308 6092 7855 8832 1203
Size: 3519 586 784 1763 977 1327
Fragments arranged by size:
3519 1763 1327 977 784 586
AgeI A'CCGG_T
Cuts at: 7787 7787
Size: 8956
AhdI GACnn_n'nnGTC
Cuts at: 3822 7685 3822
Size: 3863 5093
AleI CACnn'nnGTG
Cuts at: 1166 3590 1166
Size: 2424 6532
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4651 1184
Size: 3467 5489
AlwNI CAG_nnn'CTG
Cuts at: 3061 6699 8271 3061
Size: 3638 1572 3746
Fragments arranged by size:
3746 3638 1572
ApaLI G'TGCA_C
Cuts at: 7013 8169 7013
Size: 1156 7800
ApoI r'AATT_y
Cuts at: 1493 2096 2423 2824 2873 3346 3364 3385 3901
Size: 603 327 401 49 473 18 21 516
Cuts at: 3901 3989 5584 6265 6491 6643 8752 1493
Size: 88 1595 681 226 152 2109 1697
Fragments arranged by size:
2109 1697 1595 681 603 516 473 401
327 226 152 88 49 21 18
AseI AT'TA_AT
Cuts at: 160 1032 3149 4809 7510 160
Size: 872 2117 1660 2701 1606
Fragments arranged by size:
2701 2117 1660 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8956
AvaI C'yCGr_G
Cuts at: 1870 1943 2397 6281 1870
Size: 73 454 3884 4545
Fragments arranged by size:
4545 3884 454 73
AvrII C'CTAG_G
Cuts at: 3424 3424
Size: 8956
BamHI G'GATC_C
Cuts at: 6137 6286 6653 6137
Size: 149 367 8440
Fragments arranged by size:
8440 367 149
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 6277 7732 618
Size: 325 217 627 4490 1455 1842
Fragments arranged by size:
4490 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4743 5949 6275 1391
Size: 3352 1206 326 4072
Fragments arranged by size:
4072 3352 1206 326
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2217 3263 3714 3818 4059 5481 5814
Size: 971 318 1046 451 104 241 1422 333
Cuts at: 5814 5889 928
Size: 75 3995
Fragments arranged by size:
3995 1422 1046 971 451 333 318 241
104 75
BbvCI CC'TCA_GC
Cuts at: 3808 5940 3808
Size: 2132 6824
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7147 7181 1903
Size: 34 5210 34 3678
Fragments arranged by size:
5210 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2395 6848 8058 2395
Size: 4453 1210 3293
Fragments arranged by size:
4453 3293 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8956
BfrBI ATG'CAT
Cuts at: 3718 4895 6248 3718
Size: 1177 1353 6426
Fragments arranged by size:
6426 1353 1177
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 7567 136
Size: 107 122 71 631 605 5895 1525
Fragments arranged by size:
5895 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3022 4311 8951 3022
Size: 1289 4640 3027
Fragments arranged by size:
4640 3027 1289
BlpI GC'TnA_GC
Cuts at: 6373 6373
Size: 8956
Bme1580I G_kGCm'C
Cuts at: 1790 7017 8173 1790
Size: 5227 1156 2573
Fragments arranged by size:
5227 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 4876 1318
Size: 3558 5398
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3526 3712 6687
Size: 416 53 83 399 24 2109 186 2975
Cuts at: 6687 7645 442
Size: 958 1753
Fragments arranged by size:
2975 2109 1753 958 416 399 186 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8956
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4757 4757
Size: 8956
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2268 2901 4098 4241 4287 4364 4678 6109
Size: 1029 633 1197 143 46 77 314 1431
Cuts at: 6109 7616 1239
Size: 1507 2579
Fragments arranged by size:
2579 1507 1431 1197 1029 633 314 143
77 46
Bpu10I CC'TnA_GC
Cuts at: 2760 3808 5940 2760
Size: 1048 2132 5776
Fragments arranged by size:
5776 2132 1048
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2396 4171 5726 6147 7081 7946 8244 2396
Size: 1775 1555 421 934 865 298 3108
Fragments arranged by size:
3108 1775 1555 934 865 421 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7619 882
Size: 617 6120 2219
Fragments arranged by size:
6120 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 6747 493
Size: 711 5543 2702
Fragments arranged by size:
5543 2702 711
BsaBI GATnn'nnATC
Cuts at: 2317 2714 8950 2317
Size: 397 6236 2323
Fragments arranged by size:
6236 2323 397
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 6763 7145 7780 275
Size: 53 83 186 936 5230 382 635 1451
Fragments arranged by size:
5230 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4477 4870 7389 7787 8061 8208 8619 4477
Size: 393 2519 398 274 147 411 4814
Fragments arranged by size:
4814 2519 411 398 393 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4676 4706 5356 5386 1178
Size: 30 165 30 3273 30 650 30 4748
Fragments arranged by size:
4748 3273 650 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2629 5957 6058 1186
Size: 1443 3328 101 4084
Fragments arranged by size:
4084 3328 1443 101
BseYI C'CCAG_C
Cuts at: 8159 8655 8159
Size: 496 8460
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4023 4814 1163
Size: 573 2287 791 5305
Fragments arranged by size:
5305 2287 791 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6406 7167 8195 1540
Size: 275 186 4405 761 1028 2301
Fragments arranged by size:
4405 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3138 5949 7017 7102 8173 3138
Size: 2811 1068 85 1071 3921
Fragments arranged by size:
3921 2811 1071 1068 85
BsmI GAATG_Cn'
Cuts at: 5114 6248 6467 6560 5114
Size: 1134 219 93 7510
Fragments arranged by size:
7510 1134 219 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3138 4743 5949 6275 7017 7102 8173
Size: 399 1348 1605 1206 326 742 85 1071
Cuts at: 8173 1391
Size: 2174
Fragments arranged by size:
2174 1605 1348 1206 1071 742 399 326
85
BspEI T'CCGG_A
Cuts at: 8619 8619
Size: 8956
BspHI T'CATG_A
Cuts at: 4990 6845 8575 4990
Size: 1855 1730 5371
Fragments arranged by size:
5371 1855 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 6311 844
Size: 5467 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 6843 1394
Size: 429 5020 3507
Fragments arranged by size:
5020 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2730 7451 7625 65
Size: 1183 1482 4721 174 1396
Fragments arranged by size:
4721 1482 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 7600 7787 1700
Size: 110 5790 187 2869
Fragments arranged by size:
5790 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8956
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8956
BssSI C'ACGA_G
Cuts at: 7016 8028 7016
Size: 1012 7944
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8956
BstBI TT'CG_AA
Cuts at: 7760 7760
Size: 8956
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8956
BstXI CCAn_nnnn'nTGG
Cuts at: 4934 4934
Size: 8956
BstYI r'GATC_y
Cuts at: 1972 3022 4311 5405 6137 6286 6653 7038 7055
Size: 1050 1289 1094 732 149 367 385 17
Cuts at: 7055 8507 8593 8605 8951 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1289 1094 1050 732 385 367
346 149 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4554 5775 4554
Size: 1221 7735
BtgI C'CryG_G
Cuts at: 513 1065 2004 4713 513
Size: 552 939 2709 4756
Fragments arranged by size:
4756 2709 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2718 5856 487
Size: 683 56 1492 3138 3587
Fragments arranged by size:
3587 3138 1492 683 56
BtsI GCAGTG_nn'
Cuts at: 2547 3585 4040 4966 5599 6472 7266 7286 2547
Size: 1038 455 926 633 873 794 20 4217
Fragments arranged by size:
4217 1038 926 873 794 633 455 20
ClaI AT'CG_AT
Cuts at: 1554 2718 1554
Size: 1164 7792
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 8921
DraI TTT'AAA
Cuts at: 2116 2275 2587 5443 5559 6260 2116
Size: 159 312 2856 116 701 4812
Fragments arranged by size:
4812 2856 701 312 159 116
DraIII CAC_nnn'GTG
Cuts at: 4400 4400
Size: 8956
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7963 809
Size: 507 6647 1802
Fragments arranged by size:
6647 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3776 4494 6403
Size: 54 1425 50 275 18 1946 718 1909
Cuts at: 6403 7292 8921 8
Size: 889 1629 43
Fragments arranged by size:
1946 1909 1629 1425 889 718 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6403 1537
Size: 275 4591 4090
Fragments arranged by size:
4591 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2953 4942 5634 6002 6886 1353
Size: 1600 1989 692 368 884 3423
Fragments arranged by size:
3423 1989 1600 884 692 368
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7543 7917 8063 387
Size: 1064 138 5954 374 146 1280
Fragments arranged by size:
5954 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 5947 5947
Size: 8956
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2115 2206 2268 2757 2901 3191 4098
Size: 393 483 91 62 489 144 290 907
Cuts at: 4098 4241 4287 4364 4559 4678 5826 5901 6109
Size: 143 46 77 195 119 1148 75 208
Cuts at: 6109 7019 7616 8403 8671 1239
Size: 910 597 787 268 1524
Fragments arranged by size:
1524 1148 910 907 787 597 489 483
393 290 268 208 195 144 143 119
91 77 75 62 46
EcoNI CCTnn'n_nnAGG
Cuts at: 6031 6031
Size: 8956
EcoO109I rG'GnC_Cy
Cuts at: 2974 5315 5921 2974
Size: 2341 606 6009
Fragments arranged by size:
6009 2341 606
EcoRI G'AATT_C
Cuts at: 3346 6265 6643 3346
Size: 2919 378 5659
Fragments arranged by size:
5659 2919 378
EcoRV GAT'ATC
Cuts at: 6105 6105
Size: 8956
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2287 2596 2612 5584 2287
Size: 309 16 2972 5659
Fragments arranged by size:
5659 2972 309 16
FspI TGC'GCA
Cuts at: 7462 7462
Size: 8956
HaeII r_GCGC'y
Cuts at: 1678 8103 1678
Size: 6425 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3014 3046 3808 3840 4146
Size: 32 135 32 1442 32 762 32 306
Cuts at: 4146 4178 6187 6219 7597 7629 7671 7703 1373
Size: 32 2009 32 1378 32 42 32 2626
Fragments arranged by size:
2626 2009 1442 1378 762 306 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5350 6300 6546 669
Size: 988 281 3412 950 246 3079
Fragments arranged by size:
3412 3079 988 950 281 246
HindIII A'AGCT_T
Cuts at: 748 5747 748
Size: 4999 3957
HpaI GTT'AAC
Cuts at: 6546 6546
Size: 8956
KpnI G_GTAC'C
Cuts at: 6281 6281
Size: 8956
MfeI C'AATT_G
Cuts at: 4842 6555 4842
Size: 1713 7243
MluI A'CGCG_T
Cuts at: 8832 8832
Size: 8956
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 8070 8254 1305
Size: 6765 184 2007
Fragments arranged by size:
6765 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 7134
Fragments arranged by size:
7134 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2748 3590 4665 5091 5181 6914 7273
Size: 648 1582 842 1075 426 90 1733 359
Cuts at: 7273 7432 518
Size: 159 2042
Fragments arranged by size:
2042 1733 1582 1075 842 648 426 359
159 90
MspA1I CmG'CkG
Cuts at: 1606 3214 3749 4537 5879 5939 6372 7049 8197
Size: 1608 535 788 1342 60 433 677 1148
Cuts at: 8197 8442 1606
Size: 245 2120
Fragments arranged by size:
2120 1608 1342 1148 788 677 535 433
245 60
NcoI C'CATG_G
Cuts at: 513 1065 2004 4713 513
Size: 552 939 2709 4756
Fragments arranged by size:
4756 2709 939 552
NdeI CA'TA_TG
Cuts at: 387 4666 6244 387
Size: 4279 1578 3099
Fragments arranged by size:
4279 3099 1578
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8956
NotI GC'GGCC_GC
Cuts at: 6403 6403
Size: 8956
NsiI A_TGCA'T
Cuts at: 3720 4897 6250 3720
Size: 1177 1353 6426
Fragments arranged by size:
6426 1353 1177
NspI r_CATG'y
Cuts at: 1132 1229 5312 6096 6314 7859 1132
Size: 97 4083 784 218 1545 2229
Fragments arranged by size:
4083 2229 1545 784 218 97
PasI CC'CwG_GG
Cuts at: 1348 4509 5894 1348
Size: 3161 1385 4410
Fragments arranged by size:
4410 3161 1385
PciI A'CATG_T
Cuts at: 5308 6092 7855 5308
Size: 784 1763 6409
Fragments arranged by size:
6409 1763 784
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8956
PmeI GTTT'AAAC
Cuts at: 6260 6260
Size: 8956
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7025 7057 8538 8570 7025
Size: 32 1481 32 7411
Fragments arranged by size:
7411 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2974 5315 2974
Size: 2341 6615
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 8956
PsiI TTA'TAA
Cuts at: 5018 5220 6526 5018
Size: 202 1306 7448
Fragments arranged by size:
7448 1306 202
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5250 5282 5250
Size: 32 8924
PstI C_TGCA'G
Cuts at: 830 6308 830
Size: 5478 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8956
PvuII CAG'CTG
Cuts at: 3749 4537 5879 3749
Size: 788 1342 6826
Fragments arranged by size:
6826 1342 788
SacI G_AGCT'C
Cuts at: 5949 5949
Size: 8956
SalI G'TCGA_C
Cuts at: 1936 5348 6298 1936
Size: 3412 950 4594
Fragments arranged by size:
4594 3412 950
SbfI CC_TGCA'GG
Cuts at: 6308 6308
Size: 8956
ScaI AGT'ACT
Cuts at: 7204 8916 7204
Size: 1712 7244
SfcI C'TryA_G
Cuts at: 826 1045 2030 3339 6304 7439 8120 8311 826
Size: 219 985 1309 2965 1135 681 191 1471
Fragments arranged by size:
2965 1471 1309 1135 985 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8956
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8956
SmaI CCC'GGG
Cuts at: 1872 2399 6283 1872
Size: 527 3884 4545
Fragments arranged by size:
4545 3884 527
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2411 4150 5741 6162 6233 7060
Size: 197 926 468 1739 1591 421 71 827
Cuts at: 7060 7961 8223 820
Size: 901 262 1553
Fragments arranged by size:
1739 1591 1553 926 901 827 468 421
262 197 71
SnaBI TAC'GTA
Cuts at: 493 6747 493
Size: 6254 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8956
SphI G_CATG'C
Cuts at: 1132 6314 1132
Size: 5182 3774
SspI AAT'ATT
Cuts at: 5 52 4249 5722 6880 5
Size: 47 4197 1473 1158 2081
Fragments arranged by size:
4197 2081 1473 1158 47
StuI AGG'CCT
Cuts at: 1517 3298 3472 1517
Size: 1781 174 7001
Fragments arranged by size:
7001 1781 174
StyI C'CwwG_G
Cuts at: 513 1065 2004 3424 4191 4713 6069 6395 513
Size: 552 939 1420 767 522 1356 326 3074
Fragments arranged by size:
3074 1420 1356 939 767 552 522 326
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4677 5193 6983 7000 7153 7338 7783
Size: 1255 3097 516 1790 17 153 185 445
Cuts at: 7783 325
Size: 1498
Fragments arranged by size:
3097 1790 1498 1255 516 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5542 7202 8914
Size: 274 80 33 51 1443 3565 1660 1712
Cuts at: 8914 96
Size: 138
Fragments arranged by size:
3565 1712 1660 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4489 4521 5356 5388 8916 8948 4489
Size: 32 835 32 3528 32 4497
Fragments arranged by size:
4497 3528 835 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8956
XbaI T'CTAG_A
Cuts at: 6292 6292
Size: 8956
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 6187 6187
Size: 8956
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8956
XmaI C'CCGG_G
Cuts at: 1870 2397 6281 1870
Size: 527 3884 4545
Fragments arranged by size:
4545 3884 527
XmnI GAAnn'nnTTC
Cuts at: 3204 7085 3204
Size: 3881 5075
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6764 276
Size: 53 83 186 936 5230 2468
Fragments arranged by size:
5230 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BsiWI
BsmBI BstZ17I FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PfoI PmlI PspOMI PspXI RsrII SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI