Restriction map for
pFN21AE9674
(Circular) (Six-base) MAPSORT of: pFN21AE9674.seq Check: 4080 from: 1 to: 7567
pFN21AE9674 7567 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3954 5377 278
Size: 53 83 186 936 2418 1423 2468
Fragments arranged by size:
2468 2418 1423 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4910 1937
Size: 2973 4594
Acc65I G'GTAC_C
Cuts at: 4888 4888
Size: 7567
AclI AA'CG_TT
Cuts at: 1563 5694 6067 1563
Size: 4131 373 3063
Fragments arranged by size:
4131 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2824 3303 3923 4143 4722 5630 7014 7282
Size: 1192 479 620 220 579 908 1384 268
Cuts at: 7282 1632
Size: 1917
Fragments arranged by size:
1917 1384 1192 908 620 579 479 268
220
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7370
AflIII A'CryG_T
Cuts at: 1203 6466 7443 1203
Size: 5263 977 1327
Fragments arranged by size:
5263 1327 977
AgeI A'CCGG_T
Cuts at: 6398 6398
Size: 7567
AhdI GACnn_n'nnGTC
Cuts at: 4803 6296 4803
Size: 1493 6074
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7567
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4107 1184
Size: 2923 4644
AlwNI CAG_nnn'CTG
Cuts at: 2908 5310 6882 2908
Size: 2402 1572 3593
Fragments arranged by size:
3593 2402 1572
ApaLI G'TGCA_C
Cuts at: 4457 5624 6780 4457
Size: 1167 1156 5244
Fragments arranged by size:
5244 1167 1156
ApoI r'AATT_y
Cuts at: 1493 2170 4829 4876 5102 5254 7363 1493
Size: 677 2659 47 226 152 2109 1697
Fragments arranged by size:
2659 2109 1697 677 226 152 47
AseI AT'TA_AT
Cuts at: 160 1032 6121 160
Size: 872 5089 1606
Fragments arranged by size:
5089 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7567
AvaI C'yCGr_G
Cuts at: 1870 1943 2116 4892 1870
Size: 73 173 2776 4545
Fragments arranged by size:
4545 2776 173 73
BamHI G'GATC_C
Cuts at: 4897 5264 4897
Size: 367 7200
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2120 3457 3936 4888 6343
Size: 325 217 627 333 1337 479 952 1455
Cuts at: 6343 618
Size: 1842
Fragments arranged by size:
1842 1455 1337 952 627 479 333 325
217
BanII G_rGCy'C
Cuts at: 1391 3521 3774 4701 4886 1391
Size: 2130 253 927 185 4072
Fragments arranged by size:
4072 2130 927 253 185
BbeI G_GCGC'C
Cuts at: 3940 3940
Size: 7567
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2671 3242 3401 928
Size: 971 772 571 159 5094
Fragments arranged by size:
5094 971 772 571 159
BbvCI CC'TCA_GC
Cuts at: 4446 4446
Size: 7567
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3517 3551 5758 5792 1903
Size: 34 1580 34 2207 34 3678
Fragments arranged by size:
3678 2207 1580 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5459 6669 5459
Size: 1210 6357
BclI T'GATC_A
Cuts at: 1548 3573 1548
Size: 2025 5542
BfrBI ATG'CAT
Cuts at: 3009 3009
Size: 7567
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6178 136
Size: 107 122 71 631 605 4506 1525
Fragments arranged by size:
4506 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4647 7562 4647
Size: 2915 4652
BlpI GC'TnA_GC
Cuts at: 4290 4410 4984 4290
Size: 120 574 6873
Fragments arranged by size:
6873 574 120
Bme1580I G_kGCm'C
Cuts at: 1790 2123 2863 3746 4461 4514 5628 6784 1790
Size: 333 740 883 715 53 1114 1156 2573
Fragments arranged by size:
2573 1156 1114 883 740 715 333 53
BmgBI CAC'GTC
Cuts at: 1318 2832 1318
Size: 1514 6053
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3555 3808 4852
Size: 416 53 83 399 24 2138 253 1044
Cuts at: 4852 5298 6256 442
Size: 446 958 1753
Fragments arranged by size:
2138 1753 1044 958 446 416 399 253
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7567
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2956 2956
Size: 7567
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2379 2654 3004 3351 3935 6227 1239
Size: 1140 275 350 347 584 2292 2579
Fragments arranged by size:
2579 2292 1140 584 350 347 275
Bpu10I CC'TnA_GC
Cuts at: 2448 4446 2448
Size: 1998 5569
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2440 3215 3926 4259 5692 6557 6855 2440
Size: 775 711 333 1433 865 298 3152
Fragments arranged by size:
3152 1433 865 775 711 333 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4577 6230 882
Size: 617 3078 1653 2219
Fragments arranged by size:
3078 2219 1653 617
BsaAI yAC'GTr
Cuts at: 493 1204 2345 4252 5358 493
Size: 711 1141 1907 1106 2702
Fragments arranged by size:
2702 1907 1141 1106 711
BsaBI GATnn'nnATC
Cuts at: 7561 7561
Size: 7567
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3937 3951 5374 5756
Size: 53 83 186 936 2404 14 1423 382
Cuts at: 5756 6391 275
Size: 635 1451
Fragments arranged by size:
2404 1451 1423 936 635 382 186 83
53 14
BsaWI w'CCGG_w
Cuts at: 3429 6000 6398 6672 6819 7230 3429
Size: 2571 398 274 147 411 3766
Fragments arranged by size:
3766 2571 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4289 4319 1178
Size: 30 165 30 2886 30 4426
Fragments arranged by size:
4426 2886 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2039 2364 4375 1186
Size: 853 325 2011 4378
Fragments arranged by size:
4378 2011 853 325
BseYI C'CCAG_C
Cuts at: 2801 3960 6770 7266 2801
Size: 1159 2810 496 3102
Fragments arranged by size:
3102 2810 1159 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 4274 1163
Size: 573 2538 4456
Fragments arranged by size:
4456 2538 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2743 5017 5778 6806 1540
Size: 275 186 742 2274 761 1028 2301
Fragments arranged by size:
2301 2274 1028 761 742 275 186
BsiHKAI G_wGCw'C
Cuts at: 3141 3774 4461 4701 5628 5713 6784 3141
Size: 633 687 240 927 85 1071 3924
Fragments arranged by size:
3924 1071 927 687 633 240 85
BsmI GAATG_Cn'
Cuts at: 4488 5078 5171 4488
Size: 590 93 6884
Fragments arranged by size:
6884 590 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4863 4863
Size: 7567
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2123 2863 3141 3521 3746 3774 4461
Size: 399 333 740 278 380 225 28 687
Cuts at: 4461 4514 4701 4886 5628 5713 6784 1391
Size: 53 187 185 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 740 687 399 380 333
278 225 187 185 85 53 28
BspEI T'CCGG_A
Cuts at: 7230 7230
Size: 7567
BspHI T'CATG_A
Cuts at: 2263 5456 7186 2263
Size: 3193 1730 2644
Fragments arranged by size:
3193 2644 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2907 4922 844
Size: 2063 2015 3489
Fragments arranged by size:
3489 2063 2015
BsrBI CCG'CTC
Cuts at: 1394 1823 3445 4294 5454 1394
Size: 429 1622 849 1160 3507
Fragments arranged by size:
3507 1622 1160 849 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2127 2778 6062 6236 65
Size: 1183 879 651 3284 174 1396
Fragments arranged by size:
3284 1396 1183 879 651 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2963 3703 6211 6398 1700
Size: 110 1153 740 2508 187 2869
Fragments arranged by size:
2869 2508 1153 740 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7567
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7567
BssSI C'ACGA_G
Cuts at: 3299 4499 5627 6639 3299
Size: 1200 1128 1012 4227
Fragments arranged by size:
4227 1200 1128 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7567
BstBI TT'CG_AA
Cuts at: 6371 6371
Size: 7567
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7567
BstXI CCAn_nnnn'nTGG
Cuts at: 4483 4483
Size: 7567
BstYI r'GATC_y
Cuts at: 1972 4647 4897 5264 5649 5666 7118 7204 7216
Size: 2675 250 367 385 17 1452 86 12
Cuts at: 7216 7562 1972
Size: 346 1977
Fragments arranged by size:
2675 1977 1452 385 367 346 250 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2203 2889 4482 513
Size: 552 1138 686 1593 3598
Fragments arranged by size:
3598 1593 1138 686 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3019 3400 3772 487
Size: 683 56 1793 381 372 4282
Fragments arranged by size:
4282 1793 683 381 372 56
BtsI GCAGTG_nn'
Cuts at: 2790 2956 5083 5877 5897 2790
Size: 166 2127 794 20 4460
Fragments arranged by size:
4460 2127 794 166 20
ClaI AT'CG_AT
Cuts at: 1554 4269 1554
Size: 2715 4852
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7532
DraI TTT'AAA
Cuts at: 3076 3634 4871 3076
Size: 558 1237 5772
Fragments arranged by size:
5772 1237 558
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6574 809
Size: 507 5258 1802
Fragments arranged by size:
5258 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2505 3440 4341
Size: 54 1425 50 275 18 675 935 901
Cuts at: 4341 5014 5903 7532 8
Size: 673 889 1629 43
Fragments arranged by size:
1629 1425 935 901 889 675 673 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5014 1537
Size: 275 3202 4090
Fragments arranged by size:
4090 3202 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2040 4109 4171 5497 1353
Size: 687 2069 62 1326 3423
Fragments arranged by size:
3423 2069 1326 687 62
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6154 6528 6674 387
Size: 1064 138 4565 374 146 1280
Fragments arranged by size:
4565 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3772 4699 3772
Size: 927 6640
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2379 2654 2824 3004 3303 3351 3923
Size: 393 747 275 170 180 299 48 572
Cuts at: 3923 3935 4143 4722 5630 6227 7014 7282 1239
Size: 12 208 579 908 597 787 268 1524
Fragments arranged by size:
1524 908 787 747 597 579 572 393
299 275 268 208 180 170 48 12
EcoNI CCTnn'n_nnAGG
Cuts at: 3914 4625 3914
Size: 711 6856
EcoO109I rG'GnC_Cy
Cuts at: 2401 2685 3024 3058 4300 4630 2401
Size: 284 339 34 1242 330 5338
Fragments arranged by size:
5338 1242 339 330 284 34
EcoRI G'AATT_C
Cuts at: 4876 5254 4876
Size: 378 7189
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2202 3139 4592 2202
Size: 937 1453 5177
Fragments arranged by size:
5177 1453 937
FseI GG_CCGG'CC
Cuts at: 2967 2967
Size: 7567
FspI TGC'GCA
Cuts at: 6073 6073
Size: 7567
HaeII r_GCGC'y
Cuts at: 1678 3940 6714 1678
Size: 2262 2774 2531
Fragments arranged by size:
2774 2531 2262
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2521 2553 2997 3029 3087
Size: 32 135 32 949 32 444 32 58
Cuts at: 3087 3119 3318 3350 4775 4789 4807 4821 6208
Size: 32 199 32 1425 14 18 14 1387
Cuts at: 6208 6240 6282 6314 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1425 1387 949 444 199 135 58
42 32 32 32 32 32 32 32
32 18 14 14
HincII GTy'rAC
Cuts at: 669 1657 1938 2896 4911 5157 669
Size: 988 281 958 2015 246 3079
Fragments arranged by size:
3079 2015 988 958 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7567
HpaI GTT'AAC
Cuts at: 5157 5157
Size: 7567
KasI G'GCGC_C
Cuts at: 3936 3936
Size: 7567
KpnI G_GTAC'C
Cuts at: 4892 4892
Size: 7567
MfeI C'AATT_G
Cuts at: 5166 5166
Size: 7567
MluI A'CGCG_T
Cuts at: 7443 7443
Size: 7567
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3588 3912 3972 4305 4342 6681 6865 1305
Size: 2283 324 60 333 37 2339 184 2007
Fragments arranged by size:
2339 2283 2007 333 324 184 60 37
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2507 10
Size: 54 1425 343 675 5070
Fragments arranged by size:
5070 1425 675 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2783 5525 5884 6043 518
Size: 648 1617 2742 359 159 2042
Fragments arranged by size:
2742 2042 1617 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3184 3566 3687 3964 4121 4289 4361 4517
Size: 1578 382 121 277 157 168 72 156
Cuts at: 4517 4983 5660 6808 7053 1606
Size: 466 677 1148 245 2120
Fragments arranged by size:
2120 1578 1148 677 466 382 277 245
168 157 156 121 72
NaeI GCC'GGC
Cuts at: 2965 2965
Size: 7567
NarI GG'CG_CC
Cuts at: 3937 3937
Size: 7567
NcoI C'CATG_G
Cuts at: 513 1065 2203 513
Size: 552 1138 5877
Fragments arranged by size:
5877 1138 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7567
NgoMIV G'CCGG_C
Cuts at: 2963 2963
Size: 7567
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7567
NotI GC'GGCC_GC
Cuts at: 5014 5014
Size: 7567
NsiI A_TGCA'T
Cuts at: 3011 3011
Size: 7567
NspI r_CATG'y
Cuts at: 1132 1229 3013 4337 4925 6470 1132
Size: 97 1784 1324 588 1545 2229
Fragments arranged by size:
2229 1784 1545 1324 588 97
PasI CC'CwG_GG
Cuts at: 1348 2559 4427 1348
Size: 1211 1868 4488
Fragments arranged by size:
4488 1868 1211
PciI A'CATG_T
Cuts at: 6466 6466
Size: 7567
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2832 3117 4210 4768 1108
Size: 1724 285 1093 558 3907
Fragments arranged by size:
3907 1724 1093 558 285
PfoI T'CCnGG_A
Cuts at: 4215 4701 4215
Size: 486 7081
PmeI GTTT'AAAC
Cuts at: 4871 4871
Size: 7567
PmlI CAC'GTG
Cuts at: 4252 4252
Size: 7567
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2453 2485 5636 5668 7149 7181 2453
Size: 32 3151 32 1481 32 2839
Fragments arranged by size:
3151 2839 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2401 2685 3058 4300 4630 2401
Size: 284 373 1242 330 5338
Fragments arranged by size:
5338 1242 373 330 284
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7567
PsiI TTA'TAA
Cuts at: 5137 5137
Size: 7567
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2603 2635 2603
Size: 32 7535
PstI C_TGCA'G
Cuts at: 830 2904 4045 4919 830
Size: 2074 1141 874 3478
Fragments arranged by size:
3478 2074 1141 874
PvuI CG_AT'CG
Cuts at: 2001 2743 2001
Size: 742 6825
PvuII CAG'CTG
Cuts at: 3184 3566 3964 4121 4289 4517 3184
Size: 382 398 157 168 228 6234
Fragments arranged by size:
6234 398 382 228 168 157
SacI G_AGCT'C
Cuts at: 3774 4701 3774
Size: 927 6640
SalI G'TCGA_C
Cuts at: 1936 4909 1936
Size: 2973 4594
SbfI CC_TGCA'GG
Cuts at: 4919 4919
Size: 7567
ScaI AGT'ACT
Cuts at: 5815 7527 5815
Size: 1712 5855
SexAI A'CCwGG_T
Cuts at: 4395 4395
Size: 7567
SfcI C'TryA_G
Cuts at: 826 1045 2854 2900 4041 4915 6050 6731 6922
Size: 219 1809 46 1141 874 1135 681 191
Cuts at: 6922 826
Size: 1471
Fragments arranged by size:
1809 1471 1141 1135 874 681 219 191
46
SfoI GGC'GCC
Cuts at: 3938 3938
Size: 7567
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7567
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7567
SmaI CCC'GGG
Cuts at: 1872 4894 1872
Size: 3022 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2419 3230 3941 4238 5671 6572
Size: 197 926 476 811 711 297 1433 901
Cuts at: 6572 6834 820
Size: 262 1553
Fragments arranged by size:
1553 1433 926 901 811 711 476 297
262 197
SnaBI TAC'GTA
Cuts at: 493 5358 493
Size: 4865 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7567
SphI G_CATG'C
Cuts at: 1132 3013 4337 4925 1132
Size: 1881 1324 588 3774
Fragments arranged by size:
3774 1881 1324 588
SspI AAT'ATT
Cuts at: 5 52 5491 5
Size: 47 5439 2081
Fragments arranged by size:
5439 2081 47
StuI AGG'CCT
Cuts at: 1517 3491 1517
Size: 1974 5593
StyI C'CwwG_G
Cuts at: 513 1065 2177 2203 2379 3860 4047 4062 5006
Size: 552 1112 26 176 1481 187 15 944
Cuts at: 5006 513
Size: 3074
Fragments arranged by size:
3074 1481 1112 944 552 187 176 26
15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 5594 5611 5764 5949 6394 325
Size: 1255 4014 17 153 185 445 1498
Fragments arranged by size:
4014 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 5813 7525 96
Size: 274 80 33 51 1443 3836 1712 138
Fragments arranged by size:
3836 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4289 4321 7527 7559 4289
Size: 32 3206 32 4297
Fragments arranged by size:
4297 3206 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7567
XbaI T'CTAG_A
Cuts at: 4903 4903
Size: 7567
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2756 4030 2756
Size: 1274 6293
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7567
XmaI C'CCGG_G
Cuts at: 1870 4892 1870
Size: 3022 4545
XmnI GAAnn'nnTTC
Cuts at: 4735 4756 5696 4735
Size: 21 940 6606
Fragments arranged by size:
6606 940 21
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3952 5375 276
Size: 53 83 186 936 2418 1423 2468
Fragments arranged by size:
2468 2418 1423 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI
PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BsiWI
BstZ17I Bsu36I DraIII EcoRV FspAI NruI PacI PspOMI
PspXI RsrII SacII SanDI SapI SfiI SrfI SwaI