Restriction map for
pFN21AE9672
(Circular) (Six-base) MAPSORT of: pFN21AE9672.seq Check: 8321 from: 1 to: 9199
pFN21AE9672 9199 bp
With 182 enzymes: SgfI *
March 28, 2012 12:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4162 4459 4502 5941 4162
Size: 297 43 1439 7420
Fragments arranged by size:
7420 1439 297 43
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7009 278
Size: 53 83 186 936 5473 2468
Fragments arranged by size:
5473 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6542 1937
Size: 4605 4594
Acc65I G'GTAC_C
Cuts at: 3506 6520 3506
Size: 3014 6185
AclI AA'CG_TT
Cuts at: 1563 3672 6498 7326 7699 1563
Size: 2109 2826 828 373 3063
Fragments arranged by size:
3063 2826 2109 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4985 5159 5648 7262 8646 8914 1632
Size: 3353 174 489 1614 1384 268 1917
Fragments arranged by size:
3353 1917 1614 1384 489 268 174
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9002
AflIII A'CryG_T
Cuts at: 1203 5436 8098 9075 1203
Size: 4233 2662 977 1327
Fragments arranged by size:
4233 2662 1327 977
AgeI A'CCGG_T
Cuts at: 8030 8030
Size: 9199
AhdI GACnn_n'nnGTC
Cuts at: 7928 7928
Size: 9199
AleI CACnn'nnGTG
Cuts at: 1166 3084 1166
Size: 1918 7281
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3102 1184
Size: 1918 7281
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2240 2272 5778 5810 2240
Size: 32 3506 32 5629
Fragments arranged by size:
5629 3506 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2108 3785 3943 4045 5903 6942 8514 2108
Size: 1677 158 102 1858 1039 1572 2793
Fragments arranged by size:
2793 1858 1677 1572 1039 158 102
ApaLI G'TGCA_C
Cuts at: 7256 8412 7256
Size: 1156 8043
ApoI r'AATT_y
Cuts at: 1493 2637 2668 3520 4094 5030 5306 5590 6070
Size: 1144 31 852 574 936 276 284 480
Cuts at: 6070 6145 6508 6734 6886 8995 1493
Size: 75 363 226 152 2109 1697
Fragments arranged by size:
2109 1697 1144 936 852 574 480 363
284 276 226 152 75 31
AseI AT'TA_AT
Cuts at: 160 1032 2927 7753 160
Size: 872 1895 4826 1606
Fragments arranged by size:
4826 1895 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9199
AvaI C'yCGr_G
Cuts at: 1870 1943 2522 2622 4323 4850 6251 6524 1870
Size: 73 579 100 1701 527 1401 273 4545
Fragments arranged by size:
4545 1701 1401 579 527 273 100 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3490 3523 3490
Size: 33 9166
BamHI G'GATC_C
Cuts at: 2119 2948 6529 6896 2119
Size: 829 3581 367 4422
Fragments arranged by size:
4422 3581 829 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3373 3506 4150 5857 6520
Size: 325 217 627 1586 133 644 1707 663
Cuts at: 6520 7975 618
Size: 1455 1842
Fragments arranged by size:
1842 1707 1586 1455 663 644 627 325
217 133
BanII G_rGCy'C
Cuts at: 1391 2510 2803 3462 4851 5051 5718 6518 1391
Size: 1119 293 659 1389 200 667 800 4072
Fragments arranged by size:
4072 1389 1119 800 667 659 293 200
BbeI G_GCGC'C
Cuts at: 3377 3377
Size: 9199
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3671 3747 4560 5636 6174 928
Size: 971 1772 76 813 1076 538 3953
Fragments arranged by size:
3953 1772 1076 971 813 538 76
BbvCI CC'TCA_GC
Cuts at: 5450 5850 6405 5450
Size: 400 555 8244
Fragments arranged by size:
8244 555 400
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5062 5096 6095 6129 7390 7424 1903
Size: 34 3125 34 999 34 1261 34 3678
Fragments arranged by size:
3678 3125 1261 999 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3461 7091 8301 3461
Size: 3630 1210 4359
Fragments arranged by size:
4359 3630 1210
BclI T'GATC_A
Cuts at: 1548 4386 1548
Size: 2838 6361
BfrBI ATG'CAT
Cuts at: 2433 2433
Size: 9199
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 5837 7810
Size: 107 122 71 631 605 337 3828 1973
Cuts at: 7810 136
Size: 1525
Fragments arranged by size:
3828 1973 1525 631 605 337 122 107
71
BglII A'GATC_T
Cuts at: 5483 9194 5483
Size: 3711 5488
BlpI GC'TnA_GC
Cuts at: 3831 6616 3831
Size: 2785 6414
Bme1580I G_kGCm'C
Cuts at: 1790 2962 7260 8416 1790
Size: 1172 4298 1156 2573
Fragments arranged by size:
4298 2573 1172 1156
BmgBI CAC'GTC
Cuts at: 1318 4867 1318
Size: 3549 5650
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2408 3050 3124
Size: 416 53 83 399 24 991 642 74
Cuts at: 3124 5227 6930 7888 442
Size: 2103 1703 958 1753
Fragments arranged by size:
2103 1753 1703 991 958 642 416 399
83 74 53 24
BmtI G_CTAG'C
Cuts at: 1056 5813 1056
Size: 4757 4442
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5446 5732 5446
Size: 286 8913
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2223 3440 3476 3636 3788 3944 5660 5862
Size: 984 1217 36 160 152 156 1716 202
Cuts at: 5862 6432 7859 1239
Size: 570 1427 2579
Fragments arranged by size:
2579 1716 1427 1217 984 570 202 160
156 152 36
Bpu10I CC'TnA_GC
Cuts at: 5450 5850 6050 6121 6405 5450
Size: 400 200 71 284 8244
Fragments arranged by size:
8244 400 284 200 71
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4508 4674 5702 5780 7324 8189 8487 4508
Size: 166 1028 78 1544 865 298 5220
Fragments arranged by size:
5220 1544 1028 865 298 166 78
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4380 6429 7862 882
Size: 617 2881 2049 1433 2219
Fragments arranged by size:
2881 2219 2049 1433 617
BsaAI yAC'GTr
Cuts at: 493 1204 6226 6990 493
Size: 711 5022 764 2702
Fragments arranged by size:
5022 2702 764 711
BsaBI GATnn'nnATC
Cuts at: 9193 9193
Size: 9199
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3374 4404 5415 7006
Size: 53 83 186 936 1841 1030 1011 1591
Cuts at: 7006 7388 8023 275
Size: 382 635 1451
Fragments arranged by size:
1841 1591 1451 1030 1011 936 635 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 3789 4986 7632 8030 8304 8451 8862 3789
Size: 1197 2646 398 274 147 411 4126
Fragments arranged by size:
4126 2646 1197 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2871 2901 3188 3218 3281
Size: 30 165 30 1468 30 287 30 63
Cuts at: 3281 3311 4493 4523 6188 6218 6273 6303 1178
Size: 30 1182 30 1665 30 55 30 4074
Fragments arranged by size:
4074 1665 1468 1182 287 165 63 55
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 4013 4177 4463 1186
Size: 2827 164 286 5922
Fragments arranged by size:
5922 2827 286 164
BseYI C'CCAG_C
Cuts at: 2123 2354 4596 4708 4831 5656 8402 8898 2123
Size: 231 2242 112 123 825 2746 496 2424
Fragments arranged by size:
2746 2424 2242 825 496 231 123 112
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3359 3669 3866 3929 5935 6190 1163
Size: 573 1623 310 197 63 2006 255 4172
Fragments arranged by size:
4172 2006 1623 573 310 255 197 63
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6649 7410 8438 1540
Size: 275 186 4648 761 1028 2301
Fragments arranged by size:
4648 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2510 2803 2861 3462 3905 4322 4503 4768 4851
Size: 293 58 601 443 417 181 265 83
Cuts at: 4851 5322 5718 6056 6225 7260 7345 8416 2510
Size: 471 396 338 169 1035 85 1071 3293
Fragments arranged by size:
3293 1071 1035 601 471 443 417 396
338 293 265 181 169 85 83 58
BsmI GAATG_Cn'
Cuts at: 2162 2433 4911 6710 6803 2162
Size: 271 2478 1799 93 4558
Fragments arranged by size:
4558 2478 1799 271 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4719 5281 4719
Size: 562 8637
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2510 2803 2861 2962 3462 3905 4322
Size: 399 720 293 58 101 500 443 417
Cuts at: 4322 4503 4768 4851 5051 5322 5718 6056 6225
Size: 181 265 83 200 271 396 338 169
Cuts at: 6225 6518 7260 7345 8416 1391
Size: 293 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 720 500 443 417 399
396 338 293 293 271 265 200 181
169 101 85 83 58
BspEI T'CCGG_A
Cuts at: 3789 4986 8862 3789
Size: 1197 3876 4126
Fragments arranged by size:
4126 3876 1197
BspHI T'CATG_A
Cuts at: 2773 7088 8818 2773
Size: 4315 1730 3154
Fragments arranged by size:
4315 3154 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3682 4162 4459 4502 5941 6554 844
Size: 2838 480 297 43 1439 613 3489
Fragments arranged by size:
3489 2838 1439 613 480 297 43
BsrBI CCG'CTC
Cuts at: 1394 1823 7086 1394
Size: 429 5263 3507
Fragments arranged by size:
5263 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3183 3642 5097 5484 6011 6057 7694
Size: 1183 1935 459 1455 387 527 46 1637
Cuts at: 7694 7868 65
Size: 174 1396
Fragments arranged by size:
1935 1637 1455 1396 1183 527 459 387
174 46
BsrFI r'CCGG_y
Cuts at: 1700 1810 7843 8030 1700
Size: 110 6033 187 2869
Fragments arranged by size:
6033 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2323 2719 96
Size: 2227 396 6576
Fragments arranged by size:
6576 2227 396
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9199
BssSI C'ACGA_G
Cuts at: 4805 7259 8271 4805
Size: 2454 1012 5733
Fragments arranged by size:
5733 2454 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4459 5819 5873 6022 794
Size: 3665 1360 54 149 3971
Fragments arranged by size:
3971 3665 1360 149 54
BstBI TT'CG_AA
Cuts at: 2641 8003 2641
Size: 5362 3837
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9199
BstXI CCAn_nnnn'nTGG
Cuts at: 4930 4930
Size: 9199
BstYI r'GATC_y
Cuts at: 1972 2056 2119 2213 2948 3219 3611 3849 4810
Size: 84 63 94 735 271 392 238 961
Cuts at: 4810 5483 5652 6529 6896 7281 7298 8750 8836
Size: 673 169 877 367 385 17 1452 86
Cuts at: 8836 8848 9194 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 961 877 735 673 392 385
367 346 271 238 169 94 86 84
63 17 12
Bsu36I CC'TnA_GG
Cuts at: 3571 4267 4582 4783 3571
Size: 696 315 201 7987
Fragments arranged by size:
7987 696 315 201
BtgI C'CryG_G
Cuts at: 513 1065 2004 6087 6308 513
Size: 552 939 4083 221 3404
Fragments arranged by size:
4083 3404 939 552 221
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8460
Fragments arranged by size:
8460 683 56
BtsI GCAGTG_nn'
Cuts at: 2395 3601 3686 3786 4705 6377 6416 6715 7509
Size: 1206 85 100 919 1672 39 299 794
Cuts at: 7509 7529 2395
Size: 20 4065
Fragments arranged by size:
4065 1672 1206 919 794 299 100 85
39 20
ClaI AT'CG_AT
Cuts at: 1554 5082 1554
Size: 3528 5671
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9164
DraI TTT'AAA
Cuts at: 2664 4059 6144 6503 2664
Size: 1395 2085 359 5360
Fragments arranged by size:
5360 2085 1395 359
DraIII CAC_nnn'GTG
Cuts at: 2085 2416 2717 2085
Size: 331 301 8567
Fragments arranged by size:
8567 331 301
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3995 4871 6319 8206 809
Size: 507 2679 876 1448 1887 1802
Fragments arranged by size:
2679 1887 1802 1448 876 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 5829 5908 5971
Size: 54 1425 50 275 18 3999 79 63
Cuts at: 5971 6646 7535 9164 8
Size: 675 889 1629 43
Fragments arranged by size:
3999 1629 1425 889 675 275 79 63
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6646 1537
Size: 275 4834 4090
Fragments arranged by size:
4834 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2444 4817 5135 5579 6251 7129 1353
Size: 1091 2373 318 444 672 878 3423
Fragments arranged by size:
3423 2373 1091 878 672 444 318
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7786 8160 8306 387
Size: 1064 138 6197 374 146 1280
Fragments arranged by size:
6197 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2508 2801 3460 4849 5716 2508
Size: 293 659 1389 867 5991
Fragments arranged by size:
5991 1389 867 659 293
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2223 3440 3476 3636 3788 3944 4985
Size: 393 591 1217 36 160 152 156 1041
Cuts at: 4985 5159 5648 5660 5862 6432 7262 7859 8646
Size: 174 489 12 202 570 830 597 787
Cuts at: 8646 8914 1239
Size: 268 1524
Fragments arranged by size:
1524 1217 1041 830 787 597 591 570
489 393 268 202 174 160 156 152
36 12
EcoNI CCTnn'n_nnAGG
Cuts at: 3338 3541 4192 4411 6003 6457 3338
Size: 203 651 219 1592 454 6080
Fragments arranged by size:
6080 1592 651 454 219 203
EcoO109I rG'GnC_Cy
Cuts at: 2020 2626 3772 3799 3919 3997 4705 5446 6465
Size: 606 1146 27 120 78 708 741 1019
Cuts at: 6465 2020
Size: 4754
Fragments arranged by size:
4754 1146 1019 741 708 606 120 78
27
EcoRI G'AATT_C
Cuts at: 6070 6508 6886 6070
Size: 438 378 8383
Fragments arranged by size:
8383 438 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2713 4645 4813 5522 5743 2713
Size: 1932 168 709 221 6169
Fragments arranged by size:
6169 1932 709 221 168
FspI TGC'GCA
Cuts at: 7705 7705
Size: 9199
HaeII r_GCGC'y
Cuts at: 1678 3024 3377 4013 8346 1678
Size: 1346 353 636 4333 2531
Fragments arranged by size:
4333 2531 1346 636 353
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2871 2903 3457 3489 4263
Size: 32 135 32 1299 32 554 32 774
Cuts at: 4263 4295 4472 4504 4728 4760 5331 5363 6091
Size: 32 177 32 224 32 571 32 728
Cuts at: 6091 6123 6188 6220 6273 6305 7840 7872 7914
Size: 32 65 32 53 32 1535 32 42
Cuts at: 7914 7946 1373
Size: 32 2626
Fragments arranged by size:
2626 1535 1299 774 728 571 554 224
177 135 65 53 42 32 32 32
32 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2077 3426 4402 5737 6543 6789
Size: 988 281 139 1349 976 1335 806 246
Cuts at: 6789 669
Size: 3079
Fragments arranged by size:
3079 1349 1335 988 976 806 281 246
139
HindIII A'AGCT_T
Cuts at: 748 5468 748
Size: 4720 4479
HpaI GTT'AAC
Cuts at: 6789 6789
Size: 9199
KasI G'GCGC_C
Cuts at: 3373 3373
Size: 9199
KpnI G_GTAC'C
Cuts at: 3510 6524 3510
Size: 3014 6185
MfeI C'AATT_G
Cuts at: 6798 6798
Size: 9199
MluI A'CGCG_T
Cuts at: 9075 9075
Size: 9199
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3423 3589 4536 4998 5188 6095 8313 8497
Size: 2118 166 947 462 190 907 2218 184
Cuts at: 8497 1305
Size: 2007
Fragments arranged by size:
2218 2118 2007 947 907 462 190 184
166
MscI TGG'CCA
Cuts at: 10 64 1489 1832 5910 5973 10
Size: 54 1425 343 4078 63 3236
Fragments arranged by size:
4078 3236 1425 343 63 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3084 3635 3720 4895 4925 4928 4955
Size: 648 1918 551 85 1175 30 3 27
Cuts at: 4955 5823 7157 7516 7675 518
Size: 868 1334 359 159 2042
Fragments arranged by size:
2042 1918 1334 1175 868 648 551 359
159 85 30 27 3
MspA1I CmG'CkG
Cuts at: 1606 2053 2354 3145 3940 4397 4600 5746 5791
Size: 447 301 791 795 457 203 1146 45
Cuts at: 5791 6615 7292 8440 8685 1606
Size: 824 677 1148 245 2120
Fragments arranged by size:
2120 1148 1146 824 795 791 677 457
447 301 245 203 45
NarI GG'CG_CC
Cuts at: 3374 3374
Size: 9199
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 7708
Fragments arranged by size:
7708 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9199
NheI G'CTAG_C
Cuts at: 1052 5809 1052
Size: 4757 4442
NotI GC'GGCC_GC
Cuts at: 6646 6646
Size: 9199
NsiI A_TGCA'T
Cuts at: 2435 2435
Size: 9199
NspI r_CATG'y
Cuts at: 1132 1229 4772 5822 6557 8102 1132
Size: 97 3543 1050 735 1545 2229
Fragments arranged by size:
3543 2229 1545 1050 735 97
PasI CC'CwG_GG
Cuts at: 1348 5958 1348
Size: 4610 4589
PciI A'CATG_T
Cuts at: 8098 8098
Size: 9199
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 9199
PfoI T'CCnGG_A
Cuts at: 3453 3930 5796 3453
Size: 477 1866 6856
Fragments arranged by size:
6856 1866 477
PmeI GTTT'AAAC
Cuts at: 6503 6503
Size: 9199
PmlI CAC'GTG
Cuts at: 6226 6226
Size: 9199
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 7268 7300 8781 8813 7268
Size: 32 1481 32 7654
Fragments arranged by size:
7654 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2626 3772 3997 5446 6465 2626
Size: 1146 225 1449 1019 5360
Fragments arranged by size:
5360 1449 1146 1019 225
PshAI GACnn'nnGTC
Cuts at: 1613 3765 1613
Size: 2152 7047
PsiI TTA'TAA
Cuts at: 4112 6769 4112
Size: 2657 6542
PspXI vC'TCGA_Gb
Cuts at: 4850 4850
Size: 9199
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5210 5242 5210
Size: 32 9167
PstI C_TGCA'G
Cuts at: 830 2040 3078 3693 3759 3781 3951 4435 6458
Size: 1210 1038 615 66 22 170 484 2023
Cuts at: 6458 6551 830
Size: 93 3478
Fragments arranged by size:
3478 2023 1210 1038 615 484 170 93
66 22
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9199
PvuII CAG'CTG
Cuts at: 3145 3940 4397 4600 5746 5791 3145
Size: 795 457 203 1146 45 6553
Fragments arranged by size:
6553 1146 795 457 203 45
SacI G_AGCT'C
Cuts at: 2510 2803 3462 4851 5718 2510
Size: 293 659 1389 867 5991
Fragments arranged by size:
5991 1389 867 659 293
SalI G'TCGA_C
Cuts at: 1936 6541 1936
Size: 4605 4594
SanDI GG'GwC_CC
Cuts at: 3772 3772
Size: 9199
SapI GCTCTTCn'nnn_
Cuts at: 2444 4817 6251 2444
Size: 2373 1434 5392
Fragments arranged by size:
5392 2373 1434
SbfI CC_TGCA'GG
Cuts at: 6551 6551
Size: 9199
ScaI AGT'ACT
Cuts at: 7447 9159 7447
Size: 1712 7487
SfcI C'TryA_G
Cuts at: 826 1045 2036 2992 3074 3689 3755 3777 3947
Size: 219 991 956 82 615 66 22 170
Cuts at: 3947 4204 4431 6454 6547 7682 8363 8554 826
Size: 257 227 2023 93 1135 681 191 1471
Fragments arranged by size:
2023 1471 1135 991 956 681 615 257
227 219 191 170 93 82 66 22
SfoI GGC'GCC
Cuts at: 3375 3375
Size: 9199
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9199
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9199
SmaI CCC'GGG
Cuts at: 1872 2524 2624 4325 6526 1872
Size: 652 100 1701 2201 4545
Fragments arranged by size:
4545 2201 1701 652 100
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4523 4689 4850 5717 5759 7303
Size: 197 926 2580 166 161 867 42 1544
Cuts at: 7303 8204 8466 820
Size: 901 262 1553
Fragments arranged by size:
2580 1553 1544 926 901 867 262 197
166 161 42
SnaBI TAC'GTA
Cuts at: 493 6990 493
Size: 6497 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9199
SphI G_CATG'C
Cuts at: 1132 6557 1132
Size: 5425 3774
SspI AAT'ATT
Cuts at: 5 52 2608 2925 5605 7123 5
Size: 47 2556 317 2680 1518 2081
Fragments arranged by size:
2680 2556 2081 1518 317 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9199
StyI C'CwwG_G
Cuts at: 513 1065 2004 2023 3090 3300 5529 6638 513
Size: 552 939 19 1067 210 2229 1109 3074
Fragments arranged by size:
3074 2229 1109 1067 939 552 210 19
SwaI ATTT'AAAT
Cuts at: 4059 4059
Size: 9199
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2427 2566 4117 4207 5106 5971 7226
Size: 1255 847 139 1551 90 899 865 1255
Cuts at: 7226 7243 7396 7581 8026 325
Size: 17 153 185 445 1498
Fragments arranged by size:
1551 1498 1255 1255 899 865 847 445
185 153 139 90 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2264 2323 2719
Size: 274 80 33 51 1443 287 59 396
Cuts at: 2719 4483 4569 5217 7445 9157 96
Size: 1764 86 648 2228 1712 138
Fragments arranged by size:
2228 1764 1712 1443 648 396 287 274
138 86 80 59 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3731 3763 4493 4525 9159 9191 3731
Size: 32 730 32 4634 32 3739
Fragments arranged by size:
4634 3739 730 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 6316 1316
Size: 5000 4199
XbaI T'CTAG_A
Cuts at: 4378 5314 6535 4378
Size: 936 1221 7042
Fragments arranged by size:
7042 1221 936
XhoI C'TCGA_G
Cuts at: 1943 4850 1943
Size: 2907 6292
XmaI C'CCGG_G
Cuts at: 1870 2522 2622 4323 6524 1870
Size: 652 100 1701 2201 4545
Fragments arranged by size:
4545 2201 1701 652 100
XmnI GAAnn'nnTTC
Cuts at: 7328 7328
Size: 9199
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7007 276
Size: 53 83 186 936 5473 2468
Fragments arranged by size:
5473 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PstI PvuI PvuII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI SwaI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BsiWI BstZ17I EcoRV FseI
FspAI NaeI NgoMIV NruI PacI PspOMI RsrII SacII
SexAI SfiI SrfI XcmI