Restriction map for
pFN21AE9667
(Circular) (Six-base) MAPSORT of: pFN21AE9667.seq Check: 542 from: 1 to: 8383
pFN21AE9667 8383 bp
With 182 enzymes: SgfI *
September 1, 2019 16:46 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6193 278
Size: 53 83 186 936 4657 2468
Fragments arranged by size:
4657 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4245 5726 1937
Size: 2308 1481 4594
Fragments arranged by size:
4594 2308 1481
Acc65I G'GTAC_C
Cuts at: 5426 5704 5426
Size: 278 8105
AclI AA'CG_TT
Cuts at: 1563 6510 6883 1563
Size: 4947 373 3063
Fragments arranged by size:
4947 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2859 3527 3672 3801 4107 5633 6446 7830
Size: 1227 668 145 129 306 1526 813 1384
Cuts at: 7830 8098 1632
Size: 268 1917
Fragments arranged by size:
1917 1526 1384 1227 813 668 306 268
145 129
AflII C'TTAA_G
Cuts at: 820 1017 2126 820
Size: 197 1109 7077
Fragments arranged by size:
7077 1109 197
AflIII A'CryG_T
Cuts at: 1203 7282 8259 1203
Size: 6079 977 1327
Fragments arranged by size:
6079 1327 977
AgeI A'CCGG_T
Cuts at: 2167 4551 7214 2167
Size: 2384 2663 3336
Fragments arranged by size:
3336 2663 2384
AhdI GACnn_n'nnGTC
Cuts at: 7112 7112
Size: 8383
AleI CACnn'nnGTG
Cuts at: 1166 5012 1166
Size: 3846 4537
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2372 1184
Size: 1188 7195
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3924 3956 3924
Size: 32 8351
AlwNI CAG_nnn'CTG
Cuts at: 2401 6126 7698 2401
Size: 3725 1572 3086
Fragments arranged by size:
3725 3086 1572
ApaLI G'TGCA_C
Cuts at: 6440 7596 6440
Size: 1156 7227
ApoI r'AATT_y
Cuts at: 1493 2138 2953 3611 3896 4232 5692 5918 6070
Size: 645 815 658 285 336 1460 226 152
Cuts at: 6070 8179 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1460 815 658 645 336 285
226 152
AseI AT'TA_AT
Cuts at: 160 1032 6937 160
Size: 872 5905 1606
Fragments arranged by size:
5905 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8383
AvaI C'yCGr_G
Cuts at: 1870 1943 2677 2985 5084 5466 5708 1870
Size: 73 734 308 2099 382 242 4545
Fragments arranged by size:
4545 2099 734 382 308 242 73
BamHI G'GATC_C
Cuts at: 2807 5713 6080 2807
Size: 2906 367 5110
Fragments arranged by size:
5110 2906 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4910 5426 5704 7159 618
Size: 325 217 627 3123 516 278 1455 1842
Fragments arranged by size:
3123 1842 1455 627 516 325 278 217
BanII G_rGCy'C
Cuts at: 1391 2316 2665 3848 3967 4161 4272 5702 1391
Size: 925 349 1183 119 194 111 1430 4072
Fragments arranged by size:
4072 1430 1183 925 349 194 119 111
BbeI G_GCGC'C
Cuts at: 4914 4914
Size: 8383
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2700 2847 3794 4969 5369 928
Size: 971 801 147 947 1175 400 3942
Fragments arranged by size:
3942 1175 971 947 801 400 147
BbvCI CC'TCA_GC
Cuts at: 2030 2030
Size: 8383
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5065 5099 6574 6608 1903
Size: 34 3128 34 1475 34 3678
Fragments arranged by size:
3678 3128 1475 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2030 3410 6275 7485 2030
Size: 1380 2865 1210 2928
Fragments arranged by size:
2928 2865 1380 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8383
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4037 6994 136
Size: 107 122 71 631 605 2365 2957 1525
Fragments arranged by size:
2957 2365 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2722 8378 2722
Size: 5656 2727
BlpI GC'TnA_GC
Cuts at: 4544 5800 4544
Size: 1256 7127
Bme1580I G_kGCm'C
Cuts at: 1790 5158 6444 7600 1790
Size: 3368 1286 1156 2573
Fragments arranged by size:
3368 2573 1286 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 8383
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2106 3535 3972
Size: 416 53 83 399 24 689 1429 437
Cuts at: 3972 4424 5672 6114 7072 442
Size: 452 1248 442 958 1753
Fragments arranged by size:
1753 1429 1248 958 689 452 442 437
416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8383
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3460 4715 4865 3460
Size: 1255 150 6978
Fragments arranged by size:
6978 1255 150
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2230 2300 3117 5432 7043 1239
Size: 991 70 817 2315 1611 2579
Fragments arranged by size:
2579 2315 1611 991 817 70
Bpu10I CC'TnA_GC
Cuts at: 2030 2754 4833 2030
Size: 724 2079 5580
Fragments arranged by size:
5580 2079 724
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2129 2750 2897 3884 4456 6508 7373 7671 2129
Size: 621 147 987 572 2052 865 298 2841
Fragments arranged by size:
2841 2052 987 865 621 572 298 147
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2605 3143 5476 7046 882
Size: 617 1106 538 2333 1570 2219
Fragments arranged by size:
2333 2219 1570 1106 617 538
BsaAI yAC'GTr
Cuts at: 493 1204 2345 5067 6174 493
Size: 711 1141 2722 1107 2702
Fragments arranged by size:
2722 2702 1141 1107 711
BsaBI GATnn'nnATC
Cuts at: 3095 8377 3095
Size: 5282 3101
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4911 6190 6572 7207
Size: 53 83 186 936 3378 1279 382 635
Cuts at: 7207 275
Size: 1451
Fragments arranged by size:
3378 1451 1279 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2167 2689 4253 4551 6816 7214 7488 7635 8046
Size: 522 1564 298 2265 398 274 147 411
Cuts at: 8046 2167
Size: 2504
Fragments arranged by size:
2504 2265 1564 522 411 398 298 274
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2190 2220 2687 2717 2834
Size: 30 165 30 787 30 467 30 117
Cuts at: 2834 2864 3037 3067 4196 4226 4704 4734 1178
Size: 30 173 30 1129 30 478 30 4827
Fragments arranged by size:
4827 1129 787 478 467 173 165 117
30 30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2932 4251 4454 4457 4854 1186
Size: 1746 1319 203 3 397 4715
Fragments arranged by size:
4715 1746 1319 397 203 3
BseYI C'CCAG_C
Cuts at: 3646 3992 4312 5140 7586 8082 3646
Size: 346 320 828 2446 496 3947
Fragments arranged by size:
3947 2446 828 496 346 320
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 7810
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4600 4939 5833 6594 7622 1540
Size: 275 186 2599 339 894 761 1028 2301
Fragments arranged by size:
2599 2301 1028 894 761 339 275 186
BsiHKAI G_wGCw'C
Cuts at: 2070 3991 4161 6444 6529 7600 2070
Size: 1921 170 2283 85 1071 2853
Fragments arranged by size:
2853 2283 1921 1071 170 85
BsmI GAATG_Cn'
Cuts at: 5076 5894 5987 5076
Size: 818 93 7472
Fragments arranged by size:
7472 818 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3908 5576 3908
Size: 1668 6715
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2070 2316 2665 3848 3967 3991 4161
Size: 399 280 246 349 1183 119 24 170
Cuts at: 4161 4272 5158 5702 6444 6529 7600 1391
Size: 111 886 544 742 85 1071 2174
Fragments arranged by size:
2174 1183 1071 886 742 544 399 349
280 246 170 119 111 85 24
BspEI T'CCGG_A
Cuts at: 4253 8046 4253
Size: 3793 4590
BspHI T'CATG_A
Cuts at: 6272 8002 6272
Size: 1730 6653
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4837 5738 844
Size: 3993 901 3489
Fragments arranged by size:
3993 3489 901
BsrBI CCG'CTC
Cuts at: 1394 1823 3131 4212 6270 1394
Size: 429 1308 1081 2058 3507
Fragments arranged by size:
3507 2058 1308 1081 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6878 7052 65
Size: 1183 5630 174 1396
Fragments arranged by size:
5630 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2167 4551 7027 7214 1700
Size: 110 357 2384 2476 187 2869
Fragments arranged by size:
2869 2476 2384 357 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 8383
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8383
BssSI C'ACGA_G
Cuts at: 4160 4265 6443 7455 4160
Size: 105 2178 1012 5088
Fragments arranged by size:
5088 2178 1012 105
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4361 794
Size: 3567 4816
BstBI TT'CG_AA
Cuts at: 7187 7187
Size: 8383
BstEII G'GTnAC_C
Cuts at: 1184 3214 4423 5207 1184
Size: 2030 1209 784 4360
Fragments arranged by size:
4360 2030 1209 784
BstXI CCAn_nnnn'nTGG
Cuts at: 2093 4066 4186 2093
Size: 1973 120 6290
Fragments arranged by size:
6290 1973 120
BstYI r'GATC_y
Cuts at: 1972 2102 2430 2722 2807 2869 5415 5530 5713
Size: 130 328 292 85 62 2546 115 183
Cuts at: 5713 6080 6465 6482 7934 8020 8032 8378 1972
Size: 367 385 17 1452 86 12 346 1977
Fragments arranged by size:
2546 1977 1452 385 367 346 328 292
183 130 115 86 85 62 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2012 3467 4065 4216 5450 513
Size: 552 947 1455 598 151 1234 3446
Fragments arranged by size:
3446 1455 1234 947 598 552 151
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7644
Fragments arranged by size:
7644 683 56
BtsI GCAGTG_nn'
Cuts at: 2192 3857 3963 4890 5899 6693 6713 2192
Size: 1665 106 927 1009 794 20 3862
Fragments arranged by size:
3862 1665 1009 927 794 106 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8383
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3718 3753 3844 3879 5022 5057 529
Size: 35 3154 35 91 35 1143 35 3855
Fragments arranged by size:
3855 3154 1143 91 35 35 35 35
DraI TTT'AAA
Cuts at: 3610 5687 3610
Size: 2077 6306
DraIII CAC_nnn'GTG
Cuts at: 2345 3416 2345
Size: 1071 7312
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2646 2793 3796 4649 7390 809
Size: 507 1330 147 1003 853 2741 1802
Fragments arranged by size:
2741 1802 1330 1003 853 507 147
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2219 2892 3358
Size: 54 1425 50 275 18 389 673 466
Cuts at: 3358 3631 3672 4017 4597 4611 5586 5830 6719
Size: 273 41 345 580 14 975 244 889
Cuts at: 6719 8348 8
Size: 1629 43
Fragments arranged by size:
1629 1425 975 889 673 580 466 389
345 275 273 244 54 50 43 41
18 14
EagI C'GGCC_G
Cuts at: 1537 1812 4597 5830 1537
Size: 275 2785 1233 4090
Fragments arranged by size:
4090 2785 1233 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2442 2930 3836 6313 1353
Size: 1089 488 906 2477 3423
Fragments arranged by size:
3423 2477 1089 906 488
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6970 7344 7490 387
Size: 1064 138 5381 374 146 1280
Fragments arranged by size:
5381 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4159 4159
Size: 8383
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2230 2300 2859 3117 3527 3672 3801
Size: 393 598 70 559 258 410 145 129
Cuts at: 3801 4107 5432 5633 6446 7043 7830 8098 1239
Size: 306 1325 201 813 597 787 268 1524
Fragments arranged by size:
1524 1325 813 787 598 597 559 410
393 306 268 258 201 145 129 70
EcoNI CCTnn'n_nnAGG
Cuts at: 2097 2978 2097
Size: 881 7502
EcoO109I rG'GnC_Cy
Cuts at: 3920 4039 4476 3920
Size: 119 437 7827
Fragments arranged by size:
7827 437 119
EcoRI G'AATT_C
Cuts at: 3896 4232 5692 6070 3896
Size: 336 1460 378 6209
Fragments arranged by size:
6209 1460 378 336
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2744 2891 5614 2744
Size: 147 2723 5513
Fragments arranged by size:
5513 2723 147
FspI TGC'GCA
Cuts at: 4324 6889 4324
Size: 2565 5818
HaeII r_GCGC'y
Cuts at: 1678 4914 7530 1678
Size: 3236 2616 2531
Fragments arranged by size:
3236 2616 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2687 2719 2834 2866 3037
Size: 32 135 32 1115 32 115 32 171
Cuts at: 3037 3069 3764 3796 4489 4521 4704 4736 4746
Size: 32 695 32 693 32 183 32 10
Cuts at: 4746 4778 7024 7056 7098 7130 1373
Size: 32 2246 32 42 32 2626
Fragments arranged by size:
2626 2246 1115 695 693 183 171 135
115 42 32 32 32 32 32 32
32 32 32 32 32 10
HincII GTy'rAC
Cuts at: 669 1657 1938 3346 4246 5163 5308 5383 5727
Size: 988 281 1408 900 917 145 75 344
Cuts at: 5727 5973 669
Size: 246 3079
Fragments arranged by size:
3079 1408 988 917 900 344 281 246
145 75
HindIII A'AGCT_T
Cuts at: 748 748
Size: 8383
HpaI GTT'AAC
Cuts at: 5973 5973
Size: 8383
KasI G'GCGC_C
Cuts at: 4910 4910
Size: 8383
KpnI G_GTAC'C
Cuts at: 5430 5708 5430
Size: 278 8105
MfeI C'AATT_G
Cuts at: 5982 5982
Size: 8383
MluI A'CGCG_T
Cuts at: 8259 8259
Size: 8383
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7497 7681 1305
Size: 6192 184 2007
Fragments arranged by size:
6192 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3360 3674 10
Size: 54 1425 343 1528 314 4719
Fragments arranged by size:
4719 1528 1425 343 314 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3318 3437 4622 5012 6341 6700 6859
Size: 648 2152 119 1185 390 1329 359 159
Cuts at: 6859 518
Size: 2042
Fragments arranged by size:
2152 2042 1329 1185 648 390 359 159
119
MspA1I CmG'CkG
Cuts at: 1606 2014 2034 2628 2775 3137 3505 3522 3650
Size: 408 20 594 147 362 368 17 128
Cuts at: 3650 5799 6476 7624 7869 1606
Size: 2149 677 1148 245 2120
Fragments arranged by size:
2149 2120 1148 677 594 408 368 362
245 147 128 20 17
NarI GG'CG_CC
Cuts at: 4911 4911
Size: 8383
NcoI C'CATG_G
Cuts at: 513 1065 4065 4216 513
Size: 552 3000 151 4680
Fragments arranged by size:
4680 3000 552 151
NdeI CA'TA_TG
Cuts at: 387 3059 387
Size: 2672 5711
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8383
NotI GC'GGCC_GC
Cuts at: 5830 5830
Size: 8383
NspI r_CATG'y
Cuts at: 1132 1229 5741 7286 1132
Size: 97 4512 1545 2229
Fragments arranged by size:
4512 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2286 1348
Size: 938 7445
PciI A'CATG_T
Cuts at: 7282 7282
Size: 8383
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 8383
PfoI T'CCnGG_A
Cuts at: 2394 2442 4748 5357 5418 2394
Size: 48 2306 609 61 5359
Fragments arranged by size:
5359 2306 609 61 48
PmeI GTTT'AAAC
Cuts at: 5687 5687
Size: 8383
PmlI CAC'GTG
Cuts at: 2345 2345
Size: 8383
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4259 4291 6452 6484 7965 7997 4259
Size: 32 2161 32 1481 32 4645
Fragments arranged by size:
4645 2161 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 4476 4476
Size: 8383
PshAI GACnn'nnGTC
Cuts at: 1613 5378 1613
Size: 3765 4618
PsiI TTA'TAA
Cuts at: 5953 5953
Size: 8383
PstI C_TGCA'G
Cuts at: 830 3796 4934 5178 5735 830
Size: 2966 1138 244 557 3478
Fragments arranged by size:
3478 2966 1138 557 244
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8383
PvuII CAG'CTG
Cuts at: 3137 3505 3650 3137
Size: 368 145 7870
Fragments arranged by size:
7870 368 145
SacI G_AGCT'C
Cuts at: 4161 4161
Size: 8383
SacII CC_GC'GG
Cuts at: 2015 2015
Size: 8383
SalI G'TCGA_C
Cuts at: 1936 4244 5725 1936
Size: 2308 1481 4594
Fragments arranged by size:
4594 2308 1481
SapI GCTCTTCn'nnn_
Cuts at: 2442 3836 2442
Size: 1394 6989
SbfI CC_TGCA'GG
Cuts at: 5735 5735
Size: 8383
ScaI AGT'ACT
Cuts at: 2235 6631 8343 2235
Size: 4396 1712 2275
Fragments arranged by size:
4396 2275 1712
SexAI A'CCwGG_T
Cuts at: 3412 3412
Size: 8383
SfcI C'TryA_G
Cuts at: 826 1045 2558 3034 3451 3792 4774 4930 5110
Size: 219 1513 476 417 341 982 156 180
Cuts at: 5110 5174 5731 6866 7547 7738 826
Size: 64 557 1135 681 191 1471
Fragments arranged by size:
1513 1471 1135 982 681 557 476 417
341 219 191 180 156 64
SfoI GGC'GCC
Cuts at: 4912 4912
Size: 8383
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8383
SgrAI Cr'CCGG_yG
Cuts at: 1700 2167 4551 1700
Size: 467 2384 5532
Fragments arranged by size:
5532 2384 467
SmaI CCC'GGG
Cuts at: 1872 5468 5710 1872
Size: 3596 242 4545
Fragments arranged by size:
4545 3596 242
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2126 2144 2729 2876 3863 4471
Size: 197 926 183 18 585 147 987 608
Cuts at: 4471 6487 7388 7650 820
Size: 2016 901 262 1553
Fragments arranged by size:
2016 1553 987 926 901 608 585 262
197 183 147 18
SnaBI TAC'GTA
Cuts at: 493 6174 493
Size: 5681 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8383
SphI G_CATG'C
Cuts at: 1132 5741 1132
Size: 4609 3774
SspI AAT'ATT
Cuts at: 5 52 2968 6307 5
Size: 47 2916 3339 2081
Fragments arranged by size:
3339 2916 2081 47
StuI AGG'CCT
Cuts at: 1517 5610 1517
Size: 4093 4290
StyI C'CwwG_G
Cuts at: 513 1065 2222 2656 2803 2862 2979 3729 4065
Size: 552 1157 434 147 59 117 750 336
Cuts at: 4065 4216 5010 5221 5316 5822 513
Size: 151 794 211 95 506 3074
Fragments arranged by size:
3074 1157 794 750 552 506 434 336
211 151 147 117 95 59
SwaI ATTT'AAAT
Cuts at: 3610 3610
Size: 8383
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2060 4178 4637 6410 6427 6580 6765
Size: 1255 480 2118 459 1773 17 153 185
Cuts at: 6765 7210 325
Size: 445 1498
Fragments arranged by size:
2118 1773 1498 1255 480 459 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2233 6629 8341
Size: 274 80 33 51 1443 256 4396 1712
Cuts at: 8341 96
Size: 138
Fragments arranged by size:
4396 1712 1443 274 256 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2190 2222 4666 4698 8343 8375 2190
Size: 32 2444 32 3645 32 2198
Fragments arranged by size:
3645 2444 2198 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2188 4867 1316
Size: 872 2679 4832
Fragments arranged by size:
4832 2679 872
XbaI T'CTAG_A
Cuts at: 5719 5719
Size: 8383
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2860 3226 4000 4292 5318 5437 2860
Size: 366 774 292 1026 119 5806
Fragments arranged by size:
5806 1026 774 366 292 119
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8383
XmaI C'CCGG_G
Cuts at: 1870 5466 5708 1870
Size: 3596 242 4545
Fragments arranged by size:
4545 3596 242
XmnI GAAnn'nnTTC
Cuts at: 2724 5597 6512 2724
Size: 2873 915 4595
Fragments arranged by size:
4595 2873 915
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6191 276
Size: 53 83 186 936 4657 2468
Fragments arranged by size:
4657 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NarI NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SacII SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BfrBI BsiWI
BstZ17I Bsu36I EcoRV FseI FspAI NaeI NgoMIV NruI
NsiI PacI PspOMI PspXI PsrI RsrII SanDI SfiI
SrfI