Restriction map for
pFN21AE9663
(Circular) (Six-base) MAPSORT of: pFN21AE9663.seq Check: 1502 from: 1 to: 5821
pFN21AE9663 5821 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2548 2548
Size: 5821
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2045 3631 278
Size: 53 83 186 936 509 1586 2468
Fragments arranged by size:
2468 1586 936 509 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3023 3164 1937
Size: 1086 141 4594
Fragments arranged by size:
4594 1086 141
Acc65I G'GTAC_C
Cuts at: 3142 3142
Size: 5821
AclI AA'CG_TT
Cuts at: 1563 3120 3948 4321 1563
Size: 1557 828 373 3063
Fragments arranged by size:
3063 1557 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3017 3884 5268 5536 1632
Size: 1385 867 1384 268 1917
Fragments arranged by size:
1917 1385 1384 867 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5624
AflIII A'CryG_T
Cuts at: 1203 4720 5697 1203
Size: 3517 977 1327
Fragments arranged by size:
3517 1327 977
AgeI A'CCGG_T
Cuts at: 4652 4652
Size: 5821
AhdI GACnn_n'nnGTC
Cuts at: 4550 4550
Size: 5821
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5821
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3107 1184
Size: 1923 3898
AlwNI CAG_nnn'CTG
Cuts at: 2593 3564 5136 2593
Size: 971 1572 3278
Fragments arranged by size:
3278 1572 971
ApaI G_GGCC'C
Cuts at: 2470 2766 2470
Size: 296 5525
ApaLI G'TGCA_C
Cuts at: 3878 5034 3878
Size: 1156 4665
ApoI r'AATT_y
Cuts at: 1493 3130 3356 3508 5617 1493
Size: 1637 226 152 2109 1697
Fragments arranged by size:
2109 1697 1637 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4375 160
Size: 872 3343 1606
Fragments arranged by size:
3343 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
AvaI C'yCGr_G
Cuts at: 1870 1943 2336 3146 1870
Size: 73 393 810 4545
Fragments arranged by size:
4545 810 393 73
AvrII C'CTAG_G
Cuts at: 2689 2689
Size: 5821
BamHI G'GATC_C
Cuts at: 2360 3151 3518 2360
Size: 791 367 4663
Fragments arranged by size:
4663 791 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2137 3142 4597 618
Size: 325 217 627 350 1005 1455 1842
Fragments arranged by size:
1842 1455 1005 627 350 325 217
BanII G_rGCy'C
Cuts at: 1391 2470 2766 3140 1391
Size: 1079 296 374 4072
Fragments arranged by size:
4072 1079 374 296
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4850
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4012 4046 1903
Size: 34 2075 34 3678
Fragments arranged by size:
3678 2075 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3713 4923 3713
Size: 1210 4611
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5821
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2408 4432 136
Size: 107 122 71 631 605 736 2024 1525
Fragments arranged by size:
2024 1525 736 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5816 5816
Size: 5821
BlpI GC'TnA_GC
Cuts at: 2985 3238 2985
Size: 253 5568
Bme1580I G_kGCm'C
Cuts at: 1790 2140 2470 2537 2766 3882 5038 1790
Size: 350 330 67 229 1116 1156 2573
Fragments arranged by size:
2573 1156 1116 350 330 229 67
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5821
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3552 4510 442
Size: 416 53 83 399 24 2135 958 1753
Fragments arranged by size:
2135 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2348 1056
Size: 1292 4529
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2207 2723 2799 2992 4481 1239
Size: 968 516 76 193 1489 2579
Fragments arranged by size:
2579 1489 968 516 193 76
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3946 4811 5109 3946
Size: 865 298 4658
Fragments arranged by size:
4658 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4484 882
Size: 617 2985 2219
Fragments arranged by size:
2985 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3612 493
Size: 711 2408 2702
Fragments arranged by size:
2702 2408 711
BsaBI GATnn'nnATC
Cuts at: 5815 5815
Size: 5821
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2042 3114 3628 4010
Size: 53 83 186 936 509 1072 514 382
Cuts at: 4010 4645 275
Size: 635 1451
Fragments arranged by size:
1451 1072 936 635 514 509 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2748 4254 4652 4926 5073 5484 2748
Size: 1506 398 274 147 411 3085
Fragments arranged by size:
3085 1506 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2772 2802 1178
Size: 30 165 30 1369 30 4197
Fragments arranged by size:
4197 1369 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5821
BseYI C'CCAG_C
Cuts at: 2398 5024 5520 2398
Size: 2626 496 2699
Fragments arranged by size:
2699 2626 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2871 3113 1163
Size: 573 1135 242 3871
Fragments arranged by size:
3871 1135 573 242
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3271 4032 5060 1540
Size: 275 186 1270 761 1028 2301
Fragments arranged by size:
2301 1270 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3882 3967 5038 3882
Size: 85 1071 4665
Fragments arranged by size:
4665 1071 85
BsmI GAATG_Cn'
Cuts at: 3332 3425 3332
Size: 93 5728
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2140 2470 2537 2766 3140 3882 3967
Size: 399 350 330 67 229 374 742 85
Cuts at: 3967 5038 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 374 350 330 229
85 67
BspEI T'CCGG_A
Cuts at: 2748 5484 2748
Size: 2736 3085
BspHI T'CATG_A
Cuts at: 3710 5440 3710
Size: 1730 4091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2548 3176 844
Size: 1704 628 3489
Fragments arranged by size:
3489 1704 628
BsrBI CCG'CTC
Cuts at: 1394 1823 3708 1394
Size: 429 1885 3507
Fragments arranged by size:
3507 1885 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2206 2928 4316 4490 65
Size: 1183 958 722 1388 174 1396
Fragments arranged by size:
1396 1388 1183 958 722 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2627 4465 4652 1700
Size: 110 817 1838 187 2869
Fragments arranged by size:
2869 1838 817 187 110
BsrGI T'GTAC_A
Cuts at: 96 2145 96
Size: 2049 3772
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5821
BssSI C'ACGA_G
Cuts at: 3881 4893 3881
Size: 1012 4809
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2248 794
Size: 1454 4367
BstBI TT'CG_AA
Cuts at: 4625 4625
Size: 5821
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5821
BstYI r'GATC_y
Cuts at: 1972 2360 3151 3518 3903 3920 5372 5458 5470
Size: 388 791 367 385 17 1452 86 12
Cuts at: 5470 5816 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 791 388 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2014 2076 2800 513
Size: 552 949 62 724 3534
Fragments arranged by size:
3534 949 724 552 62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5082
Fragments arranged by size:
5082 683 56
BtsI GCAGTG_nn'
Cuts at: 3337 4131 4151 3337
Size: 794 20 5007
Fragments arranged by size:
5007 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5821
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5786
DraI TTT'AAA
Cuts at: 3125 3125
Size: 5821
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2047 4828 809
Size: 507 731 2781 1802
Fragments arranged by size:
2781 1802 731 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2151 2259 2584
Size: 54 1425 50 275 18 321 108 325
Cuts at: 2584 3102 3268 4157 5786 8
Size: 518 166 889 1629 43
Fragments arranged by size:
1629 1425 889 518 325 321 275 166
108 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3268 1537
Size: 275 1456 4090
Fragments arranged by size:
4090 1456 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2265 2582 3040 3751 1353
Size: 912 317 458 711 3423
Fragments arranged by size:
3423 912 711 458 317
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2098 4408 4782 4928 387
Size: 1064 138 509 2310 374 146 1280
Fragments arranged by size:
2310 1280 1064 509 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2207 2723 2799 2992 3017 3884 4481
Size: 393 575 516 76 193 25 867 597
Cuts at: 4481 5268 5536 1239
Size: 787 268 1524
Fragments arranged by size:
1524 867 787 597 575 516 393 268
193 76 25
EcoO109I rG'GnC_Cy
Cuts at: 2466 2763 2466
Size: 297 5524
EcoRI G'AATT_C
Cuts at: 3130 3508 3130
Size: 378 5443
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2593 2593
Size: 5821
FspI TGC'GCA
Cuts at: 4327 4327
Size: 5821
HaeII r_GCGC'y
Cuts at: 1678 2517 4968 1678
Size: 839 2451 2531
Fragments arranged by size:
2531 2451 839
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2772 2804 2968 3000 4462
Size: 32 135 32 1200 32 164 32 1462
Cuts at: 4462 4494 4536 4568 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1462 1200 164 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3165 3411 669
Size: 988 281 1227 246 3079
Fragments arranged by size:
3079 1227 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5821
HpaI GTT'AAC
Cuts at: 3411 3411
Size: 5821
KpnI G_GTAC'C
Cuts at: 3146 3146
Size: 5821
MfeI C'AATT_G
Cuts at: 3420 3420
Size: 5821
MluI A'CGCG_T
Cuts at: 5697 5697
Size: 5821
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2355 4935 5119 1305
Size: 1050 2580 184 2007
Fragments arranged by size:
2580 2007 1050 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2261 2586 3104 10
Size: 54 1425 343 429 325 518 2727
Fragments arranged by size:
2727 1425 518 429 343 325 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3089 3779 4138 4297 518
Size: 648 1923 690 359 159 2042
Fragments arranged by size:
2042 1923 690 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2016 2177 2284 2952 2989 3237 3914 5062
Size: 410 161 107 668 37 248 677 1148
Cuts at: 5062 5307 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 668 410 248 245 161
107 37
NaeI GCC'GGC
Cuts at: 2629 2629
Size: 5821
NcoI C'CATG_G
Cuts at: 513 1065 2800 513
Size: 552 1735 3534
Fragments arranged by size:
3534 1735 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5821
NgoMIV G'CCGG_C
Cuts at: 2627 2627
Size: 5821
NheI G'CTAG_C
Cuts at: 1052 2344 1052
Size: 1292 4529
NotI GC'GGCC_GC
Cuts at: 3268 3268
Size: 5821
NspI r_CATG'y
Cuts at: 1132 1229 3179 4724 1132
Size: 97 1950 1545 2229
Fragments arranged by size:
2229 1950 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5821
PciI A'CATG_T
Cuts at: 4720 4720
Size: 5821
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5821
PfoI T'CCnGG_A
Cuts at: 2160 2739 2160
Size: 579 5242
PmeI GTTT'AAAC
Cuts at: 3125 3125
Size: 5821
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3890 3922 5403 5435 3890
Size: 32 1481 32 4276
Fragments arranged by size:
4276 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5821
PsiI TTA'TAA
Cuts at: 3391 3391
Size: 5821
PspOMI G'GGCC_C
Cuts at: 2466 2762 2466
Size: 296 5525
PstI C_TGCA'G
Cuts at: 830 3173 830
Size: 2343 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5821
PvuII CAG'CTG
Cuts at: 2284 2989 2284
Size: 705 5116
SacII CC_GC'GG
Cuts at: 2017 2017
Size: 5821
SalI G'TCGA_C
Cuts at: 1936 3163 1936
Size: 1227 4594
SapI GCTCTTCn'nnn_
Cuts at: 2265 2265
Size: 5821
SbfI CC_TGCA'GG
Cuts at: 3173 3173
Size: 5821
ScaI AGT'ACT
Cuts at: 4069 5781 4069
Size: 1712 4109
SfcI C'TryA_G
Cuts at: 826 1045 3169 4304 4985 5176 826
Size: 219 2124 1135 681 191 1471
Fragments arranged by size:
2124 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5821
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5821
SmaI CCC'GGG
Cuts at: 1872 3148 1872
Size: 1276 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3925 4826 5088 820
Size: 197 926 1982 901 262 1553
Fragments arranged by size:
1982 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3612 493
Size: 3119 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5821
SphI G_CATG'C
Cuts at: 1132 3179 1132
Size: 2047 3774
SspI AAT'ATT
Cuts at: 5 52 3745 5
Size: 47 3693 2081
Fragments arranged by size:
3693 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5821
StyI C'CwwG_G
Cuts at: 513 1065 2581 2689 2766 2800 3260 513
Size: 552 1516 108 77 34 460 3074
Fragments arranged by size:
3074 1516 552 460 108 77 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3848 3865 4018 4203 4648 325
Size: 1255 2268 17 153 185 445 1498
Fragments arranged by size:
2268 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2118 2145 3051
Size: 274 80 33 51 1443 141 27 906
Cuts at: 3051 4067 5779 96
Size: 1016 1712 138
Fragments arranged by size:
1712 1443 1016 906 274 141 138 80
51 33 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2868 2900 5781 5813 2868
Size: 32 2881 32 2876
Fragments arranged by size:
2881 2876 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5821
XbaI T'CTAG_A
Cuts at: 3157 3157
Size: 5821
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2371 2371
Size: 5821
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5821
XmaI C'CCGG_G
Cuts at: 1870 3146 1870
Size: 1276 4545
XmnI GAAnn'nnTTC
Cuts at: 2868 3950 2868
Size: 1082 4739
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2043 3629 276
Size: 53 83 186 936 509 1586 2468
Fragments arranged by size:
2468 1586 936 509 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SacII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI BaeI BbeI BbvCI BfrBI BplI
Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI
PacI PmlI PpuMI PspXI PsrI RsrII SacI SanDI
SexAI SfiI SfoI SrfI SwaI