Restriction map for
pFN21AE9662
(Circular) (Six-base) MAPSORT of: pFN21AE9662.seq Check: 8984 from: 1 to: 5767
pFN21AE9662 5767 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3577 278
Size: 53 83 186 936 2041 2468
Fragments arranged by size:
2468 2041 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2319 2780 3110 1937
Size: 382 461 330 4594
Fragments arranged by size:
4594 461 382 330
Acc65I G'GTAC_C
Cuts at: 2909 3088 2909
Size: 179 5588
AclI AA'CG_TT
Cuts at: 1563 3894 4267 1563
Size: 2331 373 3063
Fragments arranged by size:
3063 2331 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2079 3026 3830 5214 5482 1632
Size: 447 947 804 1384 268 1917
Fragments arranged by size:
1917 1384 947 804 447 268
AfeI AGC'GCT
Cuts at: 2878 2878
Size: 5767
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5570
AflIII A'CryG_T
Cuts at: 1203 3033 4666 5643 1203
Size: 1830 1633 977 1327
Fragments arranged by size:
1830 1633 1327 977
AgeI A'CCGG_T
Cuts at: 4598 4598
Size: 5767
AhdI GACnn_n'nnGTC
Cuts at: 4496 4496
Size: 5767
AleI CACnn'nnGTG
Cuts at: 1166 2699 1166
Size: 1533 4234
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2831 1184
Size: 1647 4120
AlwNI CAG_nnn'CTG
Cuts at: 3061 3510 5082 3061
Size: 449 1572 3746
Fragments arranged by size:
3746 1572 449
ApaI G_GGCC'C
Cuts at: 2764 2764
Size: 5767
ApaLI G'TGCA_C
Cuts at: 3824 4980 3824
Size: 1156 4611
ApoI r'AATT_y
Cuts at: 1493 3076 3302 3454 5563 1493
Size: 1583 226 152 2109 1697
Fragments arranged by size:
2109 1697 1583 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4321 160
Size: 872 3289 1606
Fragments arranged by size:
3289 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5767
AvaI C'yCGr_G
Cuts at: 1870 1943 2885 3092 1870
Size: 73 942 207 4545
Fragments arranged by size:
4545 942 207 73
BamHI G'GATC_C
Cuts at: 2733 3097 3464 2733
Size: 364 367 5036
Fragments arranged by size:
5036 367 364
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2208 2606 2909 3088 4543
Size: 325 217 627 421 398 303 179 1455
Cuts at: 4543 618
Size: 1842
Fragments arranged by size:
1842 1455 627 421 398 325 303 217
179
BanII G_rGCy'C
Cuts at: 1391 2188 2277 2437 2764 3086 1391
Size: 797 89 160 327 322 4072
Fragments arranged by size:
4072 797 327 322 160 89
BbeI G_GCGC'C
Cuts at: 2212 2212
Size: 5767
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4796
BbvCI CC'TCA_GC
Cuts at: 2418 2418
Size: 5767
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3958 3992 1903
Size: 34 50 34 1937 34 3678
Fragments arranged by size:
3678 1937 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3659 4869 3659
Size: 1210 4557
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5767
BfrBI ATG'CAT
Cuts at: 2998 2998
Size: 5767
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2441 4378 136
Size: 107 122 71 631 605 769 1937 1525
Fragments arranged by size:
1937 1525 769 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5762 5762
Size: 5767
BlpI GC'TnA_GC
Cuts at: 3184 3184
Size: 5767
Bme1580I G_kGCm'C
Cuts at: 1790 2503 2764 3828 4984 1790
Size: 713 261 1064 1156 2573
Fragments arranged by size:
2573 1156 1064 713 261
BmgBI CAC'GTC
Cuts at: 1318 2227 1318
Size: 909 4858
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3498 4456 442
Size: 416 53 83 399 24 2081 958 1753
Fragments arranged by size:
2081 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5767
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2541 2546 2651 4427 1239
Size: 1302 5 105 1776 2579
Fragments arranged by size:
2579 1776 1302 105 5
Bpu10I CC'TnA_GC
Cuts at: 2418 2418
Size: 5767
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3892 4757 5055 3892
Size: 865 298 4604
Fragments arranged by size:
4604 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4430 882
Size: 617 2931 2219
Fragments arranged by size:
2931 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2104 3558 493
Size: 711 900 1454 2702
Fragments arranged by size:
2702 1454 900 711
BsaBI GATnn'nnATC
Cuts at: 5761 5761
Size: 5767
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2209 3574 3956 4591
Size: 53 83 186 936 676 1365 382 635
Cuts at: 4591 275
Size: 1451
Fragments arranged by size:
1451 1365 936 676 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2572 2603 4200 4598 4872 5019 5430 2572
Size: 31 1597 398 274 147 411 2909
Fragments arranged by size:
2909 1597 411 398 274 147 31
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5542
Fragments arranged by size:
5542 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2435 2726 1186
Size: 1249 291 4227
Fragments arranged by size:
4227 1249 291
BseYI C'CCAG_C
Cuts at: 2140 2676 2790 4970 5466 2140
Size: 536 114 2180 496 2441
Fragments arranged by size:
2441 2180 536 496 114
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2801 3047 1163
Size: 573 1065 246 3883
Fragments arranged by size:
3883 1065 573 246
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2346 3217 3978 5006 1540
Size: 275 186 345 871 761 1028 2301
Fragments arranged by size:
2301 1028 871 761 345 275 186
BsiHKAI G_wGCw'C
Cuts at: 2188 2277 2922 3828 3913 4984 2188
Size: 89 645 906 85 1071 2971
Fragments arranged by size:
2971 1071 906 645 89 85
BsiWI C'GTAC_G
Cuts at: 2326 2326
Size: 5767
BsmI GAATG_Cn'
Cuts at: 3278 3371 3278
Size: 93 5674
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2188 2277 2437 2503 2764 2922 3086
Size: 399 398 89 160 66 261 158 164
Cuts at: 3086 3828 3913 4984 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 398 261 164 160
158 89 85 66
BspEI T'CCGG_A
Cuts at: 2572 5430 2572
Size: 2858 2909
BspHI T'CATG_A
Cuts at: 3656 5386 3656
Size: 1730 4037
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3122 844
Size: 2278 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2011 2308 2739 3654 1394
Size: 429 188 297 431 915 3507
Fragments arranged by size:
3507 915 431 429 297 188
BsrDI GCAATG_nn'
Cuts at: 65 1248 2174 4262 4436 65
Size: 1183 926 2088 174 1396
Fragments arranged by size:
2088 1396 1183 926 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2049 2682 4411 4598 1700
Size: 110 239 633 1729 187 2869
Fragments arranged by size:
2869 1729 633 239 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5767
BssHII G'CGCG_C
Cuts at: 1925 2297 1925
Size: 372 5395
BssSI C'ACGA_G
Cuts at: 2012 2836 3827 4839 2012
Size: 824 991 1012 2940
Fragments arranged by size:
2940 1012 991 824
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5767
BstBI TT'CG_AA
Cuts at: 4571 4571
Size: 5767
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5767
BstXI CCAn_nnnn'nTGG
Cuts at: 2524 2524
Size: 5767
BstYI r'GATC_y
Cuts at: 1972 2733 3097 3464 3849 3866 5318 5404 5416
Size: 761 364 367 385 17 1452 86 12
Cuts at: 5416 5762 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 761 385 367 364 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2355 2459 2355
Size: 104 5663
BtgI C'CryG_G
Cuts at: 513 1065 2199 2946 513
Size: 552 1134 747 3334
Fragments arranged by size:
3334 1134 747 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5028
Fragments arranged by size:
5028 683 56
BtsI GCAGTG_nn'
Cuts at: 3283 4077 4097 3283
Size: 794 20 4953
Fragments arranged by size:
4953 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5767
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5732
DraI TTT'AAA
Cuts at: 3071 3071
Size: 5767
DraIII CAC_nnn'GTG
Cuts at: 2227 2227
Size: 5767
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2120 4774 809
Size: 507 804 2654 1802
Fragments arranged by size:
2654 1802 804 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2383 2408 2850
Size: 54 1425 50 275 18 553 25 442
Cuts at: 2850 3027 3214 4103 5732 8
Size: 177 187 889 1629 43
Fragments arranged by size:
1629 1425 889 553 442 275 187 177
54 50 43 25 18
EagI C'GGCC_G
Cuts at: 1537 1812 3214 1537
Size: 275 1402 4090
Fragments arranged by size:
4090 1402 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2055 2867 3697 1353
Size: 702 812 830 3423
Fragments arranged by size:
3423 830 812 702
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4354 4728 4874 387
Size: 1064 138 2765 374 146 1280
Fragments arranged by size:
2765 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2186 2275 2186
Size: 89 5678
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2079 2541 2546 2651 3026 3830 4427
Size: 393 447 462 5 105 375 804 597
Cuts at: 4427 5214 5482 1239
Size: 787 268 1524
Fragments arranged by size:
1524 804 787 597 462 447 393 375
268 105 5
EcoO109I rG'GnC_Cy
Cuts at: 2137 2351 2635 2761 2137
Size: 214 284 126 5143
Fragments arranged by size:
5143 284 214 126
EcoRI G'AATT_C
Cuts at: 3076 3454 3076
Size: 378 5389
FspI TGC'GCA
Cuts at: 4273 4273
Size: 5767
HaeII r_GCGC'y
Cuts at: 1678 2078 2212 2880 4914 1678
Size: 400 134 668 2034 2531
Fragments arranged by size:
2531 2034 668 400 134
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4408 4440 4482 4514 1373
Size: 32 135 32 2836 32 42 32 2626
Fragments arranged by size:
2836 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3111 3357 669
Size: 988 281 1173 246 3079
Fragments arranged by size:
3079 1173 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5767
HpaI GTT'AAC
Cuts at: 3357 3357
Size: 5767
KasI G'GCGC_C
Cuts at: 2208 2208
Size: 5767
KpnI G_GTAC'C
Cuts at: 2913 3092 2913
Size: 179 5588
MfeI C'AATT_G
Cuts at: 3366 3366
Size: 5767
MluI A'CGCG_T
Cuts at: 5643 5643
Size: 5767
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2805 4881 5065 1305
Size: 1500 2076 184 2007
Fragments arranged by size:
2076 2007 1500 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3029 10
Size: 54 1425 343 1197 2748
Fragments arranged by size:
2748 1425 1197 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2699 2993 3725 4084 4243 518
Size: 648 1533 294 732 359 159 2042
Fragments arranged by size:
2042 1533 732 648 359 294 159
MspA1I CmG'CkG
Cuts at: 1606 2040 2120 2144 3183 3860 5008 5253 1606
Size: 434 80 24 1039 677 1148 245 2120
Fragments arranged by size:
2120 1148 1039 677 434 245 80 24
NaeI GCC'GGC
Cuts at: 2051 2051
Size: 5767
NarI GG'CG_CC
Cuts at: 2209 2209
Size: 5767
NcoI C'CATG_G
Cuts at: 513 1065 2946 513
Size: 552 1881 3334
Fragments arranged by size:
3334 1881 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5767
NgoMIV G'CCGG_C
Cuts at: 2049 2049
Size: 5767
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5767
NotI GC'GGCC_GC
Cuts at: 3214 3214
Size: 5767
NsiI A_TGCA'T
Cuts at: 3000 3000
Size: 5767
NspI r_CATG'y
Cuts at: 1132 1229 3037 3125 4670 1132
Size: 97 1808 88 1545 2229
Fragments arranged by size:
2229 1808 1545 97 88
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5767
PciI A'CATG_T
Cuts at: 3033 4666 3033
Size: 1633 4134
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2227 1108
Size: 1119 4648
PfoI T'CCnGG_A
Cuts at: 2235 2628 2235
Size: 393 5374
PmeI GTTT'AAAC
Cuts at: 3071 3071
Size: 5767
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3836 3868 5349 5381 3836
Size: 32 1481 32 4222
Fragments arranged by size:
4222 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2137 2351 2137
Size: 214 5553
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5767
PsiI TTA'TAA
Cuts at: 3337 3337
Size: 5767
PspOMI G'GGCC_C
Cuts at: 2760 2760
Size: 5767
PstI C_TGCA'G
Cuts at: 830 3119 830
Size: 2289 3478
PvuI CG_AT'CG
Cuts at: 2001 2346 2001
Size: 345 5422
PvuII CAG'CTG
Cuts at: 2120 2120
Size: 5767
SacI G_AGCT'C
Cuts at: 2188 2277 2188
Size: 89 5678
SalI G'TCGA_C
Cuts at: 1936 3109 1936
Size: 1173 4594
SapI GCTCTTCn'nnn_
Cuts at: 2867 2867
Size: 5767
SbfI CC_TGCA'GG
Cuts at: 3119 3119
Size: 5767
ScaI AGT'ACT
Cuts at: 2847 4015 5727 2847
Size: 1168 1712 2887
Fragments arranged by size:
2887 1712 1168
SexAI A'CCwGG_T
Cuts at: 2829 2829
Size: 5767
SfcI C'TryA_G
Cuts at: 826 1045 2484 3115 4250 4931 5122 826
Size: 219 1439 631 1135 681 191 1471
Fragments arranged by size:
1471 1439 1135 681 631 219 191
SfoI GGC'GCC
Cuts at: 2210 2210
Size: 5767
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5767
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5767
SmaI CCC'GGG
Cuts at: 1872 2887 3094 1872
Size: 1015 207 4545
Fragments arranged by size:
4545 1015 207
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3871 4772 5034 820
Size: 197 926 1928 901 262 1553
Fragments arranged by size:
1928 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3558 493
Size: 3065 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5767
SphI G_CATG'C
Cuts at: 1132 3125 1132
Size: 1993 3774
SspI AAT'ATT
Cuts at: 5 52 3691 5
Size: 47 3639 2081
Fragments arranged by size:
3639 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5767
StyI C'CwwG_G
Cuts at: 513 1065 2517 2946 3206 513
Size: 552 1452 429 260 3074
Fragments arranged by size:
3074 1452 552 429 260
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3794 3811 3964 4149 4594 325
Size: 1255 2214 17 153 185 445 1498
Fragments arranged by size:
2214 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2845 4013 5725
Size: 274 80 33 51 1443 868 1168 1712
Cuts at: 5725 96
Size: 138
Fragments arranged by size:
1712 1443 1168 868 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2343 2375 2380 2412 5727 5759 2343
Size: 32 5 32 3315 32 2351
Fragments arranged by size:
3315 2351 32 32 32 5
Tth111I GACn'n_nGTC
Cuts at: 1316 2228 2831 1316
Size: 912 603 4252
Fragments arranged by size:
4252 912 603
XbaI T'CTAG_A
Cuts at: 3103 3103
Size: 5767
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2011
Size: 5767
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5767
XmaI C'CCGG_G
Cuts at: 1870 2885 3092 1870
Size: 1015 207 4545
Fragments arranged by size:
4545 1015 207
XmnI GAAnn'nnTTC
Cuts at: 3896 3896
Size: 5767
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3575 276
Size: 53 83 186 936 2041 2468
Fragments arranged by size:
2468 2041 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BplI BsmBI BstZ17I
EcoNI EcoRV FalI FseI FspAI NruI PacI PmlI
PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI