Restriction map for
pFN21AE9660
(Circular) (Six-base) MAPSORT of: pFN21AE9660.seq Check: 5185 from: 1 to: 5446
pFN21AE9660 5446 bp
With 182 enzymes: SgfI *
December 28, 2011 15:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3256 278
Size: 53 83 186 936 1720 2468
Fragments arranged by size:
2468 1720 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2789 1937
Size: 852 4594
Acc65I G'GTAC_C
Cuts at: 2767 2767
Size: 5446
AclI AA'CG_TT
Cuts at: 1563 3573 3946 1563
Size: 2010 373 3063
Fragments arranged by size:
3063 2010 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2490 3509 4893 5161 1632
Size: 858 1019 1384 268 1917
Fragments arranged by size:
1917 1384 1019 858 268
AflII C'TTAA_G
Cuts at: 820 1017 2702 820
Size: 197 1685 3564
Fragments arranged by size:
3564 1685 197
AflIII A'CryG_T
Cuts at: 1203 2644 4345 5322 1203
Size: 1441 1701 977 1327
Fragments arranged by size:
1701 1441 1327 977
AgeI A'CCGG_T
Cuts at: 4277 4277
Size: 5446
AhdI GACnn_n'nnGTC
Cuts at: 4175 4175
Size: 5446
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5446
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2144 1184
Size: 960 4486
AlwNI CAG_nnn'CTG
Cuts at: 3189 4761 3189
Size: 1572 3874
ApaLI G'TGCA_C
Cuts at: 3503 4659 3503
Size: 1156 4290
ApoI r'AATT_y
Cuts at: 1493 2755 2981 3133 5242 1493
Size: 1262 226 152 2109 1697
Fragments arranged by size:
2109 1697 1262 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2420 4000 160
Size: 872 1388 1580 1606
Fragments arranged by size:
1606 1580 1388 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5446
AvaI C'yCGr_G
Cuts at: 1870 1943 2771 1870
Size: 73 828 4545
Fragments arranged by size:
4545 828 73
BamHI G'GATC_C
Cuts at: 2692 2776 3143 2692
Size: 84 367 4995
Fragments arranged by size:
4995 367 84
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2093 2767 4222 618
Size: 325 217 627 306 674 1455 1842
Fragments arranged by size:
1842 1455 674 627 325 306 217
BanII G_rGCy'C
Cuts at: 1391 2043 2765 1391
Size: 652 722 4072
Fragments arranged by size:
4072 722 652
BbeI G_GCGC'C
Cuts at: 2097 2097
Size: 5446
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2259 928
Size: 971 360 4115
Fragments arranged by size:
4115 971 360
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3637 3671 1903
Size: 34 1700 34 3678
Fragments arranged by size:
3678 1700 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3338 4548 3338
Size: 1210 4236
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5446
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2070 2102 4057
Size: 107 122 71 631 605 398 32 1955
Cuts at: 4057 136
Size: 1525
Fragments arranged by size:
1955 1525 631 605 398 122 107 71
32
BglII A'GATC_T
Cuts at: 5441 5441
Size: 5446
BlpI GC'TnA_GC
Cuts at: 2863 2863
Size: 5446
Bme1580I G_kGCm'C
Cuts at: 1790 3507 4663 1790
Size: 1717 1156 2573
Fragments arranged by size:
2573 1717 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5446
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3177 4135 442
Size: 416 53 83 399 24 1760 958 1753
Fragments arranged by size:
1760 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5446
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2193 2372 4106 1239
Size: 954 179 1734 2579
Fragments arranged by size:
2579 1734 954 179
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2744 3571 4436 4734 2744
Size: 827 865 298 3456
Fragments arranged by size:
3456 865 827 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4109 882
Size: 617 2610 2219
Fragments arranged by size:
2610 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3237 493
Size: 711 2033 2702
Fragments arranged by size:
2702 2033 711
BsaBI GATnn'nnATC
Cuts at: 2591 5440 2591
Size: 2849 2597
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2031 2094 3253 3635
Size: 53 83 186 936 498 63 1159 382
Cuts at: 3635 4270 275
Size: 635 1451
Fragments arranged by size:
1451 1159 936 635 498 382 186 83
63 53
BsaWI w'CCGG_w
Cuts at: 2051 3879 4277 4551 4698 5109 2051
Size: 1828 398 274 147 411 2388
Fragments arranged by size:
2388 1828 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5221
Fragments arranged by size:
5221 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2123 1186
Size: 838 99 4509
Fragments arranged by size:
4509 838 99
BseYI C'CCAG_C
Cuts at: 4649 5145 4649
Size: 496 4950
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2128 2319 1163
Size: 573 392 191 4290
Fragments arranged by size:
4290 573 392 191
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2896 3657 4685 1540
Size: 275 186 895 761 1028 2301
Fragments arranged by size:
2301 1028 895 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2149 2382 3507 3592 4663 2149
Size: 233 1125 85 1071 2932
Fragments arranged by size:
2932 1125 1071 233 85
BsmI GAATG_Cn'
Cuts at: 2957 3050 2957
Size: 93 5353
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2043 2149 2382 2765 3507 3592 4663
Size: 399 253 106 233 383 742 85 1071
Cuts at: 4663 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 383 253 233 106
85
BspEI T'CCGG_A
Cuts at: 2051 5109 2051
Size: 3058 2388
BspHI T'CATG_A
Cuts at: 3335 5065 3335
Size: 1730 3716
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2074 2801 844
Size: 1230 727 3489
Fragments arranged by size:
3489 1230 727
BsrBI CCG'CTC
Cuts at: 1394 1823 3333 1394
Size: 429 1510 3507
Fragments arranged by size:
3507 1510 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3941 4115 65
Size: 1183 2693 174 1396
Fragments arranged by size:
2693 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2090 4090 4277 1700
Size: 110 280 2000 187 2869
Fragments arranged by size:
2869 2000 280 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5446
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5446
BssSI C'ACGA_G
Cuts at: 3506 4518 3506
Size: 1012 4434
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5446
BstBI TT'CG_AA
Cuts at: 4250 4250
Size: 5446
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5446
BstYI r'GATC_y
Cuts at: 1972 2692 2776 3143 3528 3545 4997 5083 5095
Size: 720 84 367 385 17 1452 86 12
Cuts at: 5095 5441 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 720 385 367 346 86 84
17 12
Bsu36I CC'TnA_GG
Cuts at: 2135 2135
Size: 5446
BtgI C'CryG_G
Cuts at: 513 1065 2004 2043 513
Size: 552 939 39 3916
Fragments arranged by size:
3916 939 552 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4707
Fragments arranged by size:
4707 683 56
BtsI GCAGTG_nn'
Cuts at: 2962 3756 3776 2962
Size: 794 20 4632
Fragments arranged by size:
4632 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5446
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5411
DraI TTT'AAA
Cuts at: 2750 2750
Size: 5446
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4453 809
Size: 507 3137 1802
Fragments arranged by size:
3137 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2893 3782 5411
Size: 54 1425 50 275 18 1063 889 1629
Cuts at: 5411 8
Size: 43
Fragments arranged by size:
1629 1425 1063 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2893 1537
Size: 275 1081 4090
Fragments arranged by size:
4090 1081 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2268 2477 3376 1353
Size: 915 209 899 3423
Fragments arranged by size:
3423 915 899 209
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4033 4407 4553 387
Size: 1064 138 2444 374 146 1280
Fragments arranged by size:
2444 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2193 2372 2490 3509 4106 4893 5161
Size: 393 561 179 118 1019 597 787 268
Cuts at: 5161 1239
Size: 1524
Fragments arranged by size:
1524 1019 787 597 561 393 268 179
118
EcoRI G'AATT_C
Cuts at: 2755 3133 2755
Size: 378 5068
FspI TGC'GCA
Cuts at: 3952 3952
Size: 5446
HaeII r_GCGC'y
Cuts at: 1678 2097 4593 1678
Size: 419 2496 2531
Fragments arranged by size:
2531 2496 419
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4087 4119 4161 4193 1373
Size: 32 135 32 2515 32 42 32 2626
Fragments arranged by size:
2626 2515 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2737 2790 3036 669
Size: 988 281 799 53 246 3079
Fragments arranged by size:
3079 988 799 281 246 53
HindIII A'AGCT_T
Cuts at: 748 2303 2502 2516 748
Size: 1555 199 14 3678
Fragments arranged by size:
3678 1555 199 14
HpaI GTT'AAC
Cuts at: 3036 3036
Size: 5446
KasI G'GCGC_C
Cuts at: 2093 2093
Size: 5446
KpnI G_GTAC'C
Cuts at: 2771 2771
Size: 5446
MfeI C'AATT_G
Cuts at: 2680 3045 2680
Size: 365 5081
MluI A'CGCG_T
Cuts at: 5322 5322
Size: 5446
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4560 4744 1305
Size: 3255 184 2007
Fragments arranged by size:
3255 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3624
Fragments arranged by size:
3624 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3404 3763 3922 518
Size: 648 2238 359 159 2042
Fragments arranged by size:
2238 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2045 2862 3539 4687 4932 1606
Size: 439 817 677 1148 245 2120
Fragments arranged by size:
2120 1148 817 677 439 245
NaeI GCC'GGC
Cuts at: 2092 2092
Size: 5446
NarI GG'CG_CC
Cuts at: 2094 2094
Size: 5446
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3955
Fragments arranged by size:
3955 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5446
NgoMIV G'CCGG_C
Cuts at: 2090 2090
Size: 5446
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5446
NotI GC'GGCC_GC
Cuts at: 2893 2893
Size: 5446
NspI r_CATG'y
Cuts at: 1132 1229 2648 2804 4349 1132
Size: 97 1419 156 1545 2229
Fragments arranged by size:
2229 1545 1419 156 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5446
PciI A'CATG_T
Cuts at: 2644 4345 2644
Size: 1701 3745
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5446
PmeI GTTT'AAAC
Cuts at: 2750 2750
Size: 5446
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3515 3547 5028 5060 3515
Size: 32 1481 32 3901
Fragments arranged by size:
3901 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5446
PsiI TTA'TAA
Cuts at: 3016 3016
Size: 5446
PstI C_TGCA'G
Cuts at: 830 2721 2798 830
Size: 1891 77 3478
Fragments arranged by size:
3478 1891 77
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5446
SacII CC_GC'GG
Cuts at: 2046 2046
Size: 5446
SalI G'TCGA_C
Cuts at: 1936 2788 1936
Size: 852 4594
SbfI CC_TGCA'GG
Cuts at: 2798 2798
Size: 5446
ScaI AGT'ACT
Cuts at: 3694 5406 3694
Size: 1712 3734
SfcI C'TryA_G
Cuts at: 826 1045 2609 2717 2794 3929 4610 4801 826
Size: 219 1564 108 77 1135 681 191 1471
Fragments arranged by size:
1564 1471 1135 681 219 191 108 77
SfoI GGC'GCC
Cuts at: 2095 2095
Size: 5446
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5446
SgrAI Cr'CCGG_yG
Cuts at: 1700 2090 1700
Size: 390 5056
SmaI CCC'GGG
Cuts at: 1872 2773 1872
Size: 901 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2702 2723 3550 4451 4713 820
Size: 197 926 759 21 827 901 262 1553
Fragments arranged by size:
1553 926 901 827 759 262 197 21
SnaBI TAC'GTA
Cuts at: 493 3237 493
Size: 2744 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5446
SphI G_CATG'C
Cuts at: 1132 2804 1132
Size: 1672 3774
SspI AAT'ATT
Cuts at: 5 52 3370 5
Size: 47 3318 2081
Fragments arranged by size:
3318 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5446
StyI C'CwwG_G
Cuts at: 513 1065 2004 2885 513
Size: 552 939 881 3074
Fragments arranged by size:
3074 939 881 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3473 3490 3643 3828 4273 325
Size: 1255 1893 17 153 185 445 1498
Fragments arranged by size:
1893 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3692 5404 96
Size: 274 80 33 51 1443 1715 1712 138
Fragments arranged by size:
1715 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5406 5438 5406
Size: 32 5414
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5446
XbaI T'CTAG_A
Cuts at: 2782 2782
Size: 5446
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5446
XmaI C'CCGG_G
Cuts at: 1870 2771 1870
Size: 901 4545
XmnI GAAnn'nnTTC
Cuts at: 2712 3575 2712
Size: 863 4583
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3254 276
Size: 53 83 186 936 1720 2468
Fragments arranged by size:
2468 1720 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SacII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SanDI SapI SexAI SfiI SrfI
SwaI XcmI