Restriction map for
pFN21AE9659
(Circular) (Six-base) MAPSORT of: pFN21AE9659.seq Check: 6872 from: 1 to: 5371
pFN21AE9659 5371 bp
With 182 enzymes: SgfI *
July 6, 2012 17:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3181 278
Size: 53 83 186 936 1645 2468
Fragments arranged by size:
2468 1645 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2714 1937
Size: 777 4594
Acc65I G'GTAC_C
Cuts at: 2692 2692
Size: 5371
AclI AA'CG_TT
Cuts at: 1563 3498 3871 1563
Size: 1935 373 3063
Fragments arranged by size:
3063 1935 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2375 3434 4818 5086 1632
Size: 743 1059 1384 268 1917
Fragments arranged by size:
1917 1384 1059 743 268
AflII C'TTAA_G
Cuts at: 820 1017 2651 820
Size: 197 1634 3540
Fragments arranged by size:
3540 1634 197
AflIII A'CryG_T
Cuts at: 1203 4270 5247 1203
Size: 3067 977 1327
Fragments arranged by size:
3067 1327 977
AgeI A'CCGG_T
Cuts at: 4202 4202
Size: 5371
AhdI GACnn_n'nnGTC
Cuts at: 4100 4100
Size: 5371
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5371
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5371
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2000 2032 2000
Size: 32 5339
AlwNI CAG_nnn'CTG
Cuts at: 2506 3114 4686 2506
Size: 608 1572 3191
Fragments arranged by size:
3191 1572 608
ApaI G_GGCC'C
Cuts at: 2068 2068
Size: 5371
ApaLI G'TGCA_C
Cuts at: 3428 4584 3428
Size: 1156 4215
ApoI r'AATT_y
Cuts at: 1493 2680 2906 3058 5167 1493
Size: 1187 226 152 2109 1697
Fragments arranged by size:
2109 1697 1187 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3925 160
Size: 872 2893 1606
Fragments arranged by size:
2893 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5371
AvaI C'yCGr_G
Cuts at: 1870 1943 2696 1870
Size: 73 753 4545
Fragments arranged by size:
4545 753 73
BamHI G'GATC_C
Cuts at: 2701 3068 2701
Size: 367 5004
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2022 2336 2692 4147 618
Size: 325 217 627 235 314 356 1455 1842
Fragments arranged by size:
1842 1455 627 356 325 314 235 217
BanII G_rGCy'C
Cuts at: 1391 2068 2567 2690 1391
Size: 677 499 123 4072
Fragments arranged by size:
4072 677 499 123
BbeI G_GCGC'C
Cuts at: 2026 2026
Size: 5371
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2036 928
Size: 971 137 4263
Fragments arranged by size:
4263 971 137
BbvCI CC'TCA_GC
Cuts at: 2611 2611
Size: 5371
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3562 3596 1903
Size: 34 1625 34 3678
Fragments arranged by size:
3678 1625 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3263 4473 3263
Size: 1210 4161
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5371
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3982 136
Size: 107 122 71 631 605 2310 1525
Fragments arranged by size:
2310 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5366 5366
Size: 5371
BlpI GC'TnA_GC
Cuts at: 2788 2788
Size: 5371
Bme1580I G_kGCm'C
Cuts at: 1790 2068 2339 3432 4588 1790
Size: 278 271 1093 1156 2573
Fragments arranged by size:
2573 1156 1093 278 271
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5371
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2568 3102 4060
Size: 416 53 83 399 24 1151 534 958
Cuts at: 4060 442
Size: 1753
Fragments arranged by size:
1753 1151 958 534 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5371
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2660 4031 1239
Size: 1421 1371 2579
Fragments arranged by size:
2579 1421 1371
Bpu10I CC'TnA_GC
Cuts at: 2611 2611
Size: 5371
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2252 2267 3496 4361 4659 2252
Size: 15 1229 865 298 2964
Fragments arranged by size:
2964 1229 865 298 15
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2108 4034 882
Size: 617 609 1926 2219
Fragments arranged by size:
2219 1926 617 609
BsaAI yAC'GTr
Cuts at: 493 1204 3162 493
Size: 711 1958 2702
Fragments arranged by size:
2702 1958 711
BsaBI GATnn'nnATC
Cuts at: 5365 5365
Size: 5371
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2023 2033 3178 3560
Size: 53 83 186 936 490 10 1145 382
Cuts at: 3560 4195 275
Size: 635 1451
Fragments arranged by size:
1451 1145 936 635 490 382 186 83
53 10
BsaWI w'CCGG_w
Cuts at: 3804 4202 4476 4623 5034 3804
Size: 398 274 147 411 4141
Fragments arranged by size:
4141 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5146
Fragments arranged by size:
5146 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2054 2438 2492 1186
Size: 868 384 54 4065
Fragments arranged by size:
4065 868 384 54
BseYI C'CCAG_C
Cuts at: 2515 4574 5070 2515
Size: 2059 496 2816
Fragments arranged by size:
2816 2059 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4798
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2821 3582 4610 1540
Size: 275 186 820 761 1028 2301
Fragments arranged by size:
2301 1028 820 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3432 3517 4588 3432
Size: 85 1071 4215
Fragments arranged by size:
4215 1071 85
BsmI GAATG_Cn'
Cuts at: 2882 2975 2882
Size: 93 5278
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2068 2339 2567 2690 3432 3517 4588
Size: 399 278 271 228 123 742 85 1071
Cuts at: 4588 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 399 278 271 228 123
85
BspEI T'CCGG_A
Cuts at: 5034 5034
Size: 5371
BspHI T'CATG_A
Cuts at: 2379 3260 4990 2379
Size: 881 1730 2760
Fragments arranged by size:
2760 1730 881
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2667 2726 844
Size: 1823 59 3489
Fragments arranged by size:
3489 1823 59
BsrBI CCG'CTC
Cuts at: 1394 1823 2395 3258 1394
Size: 429 572 863 3507
Fragments arranged by size:
3507 863 572 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2133 3866 4040 65
Size: 1183 885 1733 174 1396
Fragments arranged by size:
1733 1396 1183 885 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4015 4202 1700
Size: 110 2205 187 2869
Fragments arranged by size:
2869 2205 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5371
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5371
BssSI C'ACGA_G
Cuts at: 3431 4443 3431
Size: 1012 4359
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5371
BstBI TT'CG_AA
Cuts at: 4175 4175
Size: 5371
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5371
BstXI CCAn_nnnn'nTGG
Cuts at: 2332 2332
Size: 5371
BstYI r'GATC_y
Cuts at: 1972 2271 2367 2701 3068 3453 3470 4922 5008
Size: 299 96 334 367 385 17 1452 86
Cuts at: 5008 5020 5366 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 334 299 96
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2331 2454 513
Size: 552 939 327 123 3430
Fragments arranged by size:
3430 939 552 327 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4632
Fragments arranged by size:
4632 683 56
BtsI GCAGTG_nn'
Cuts at: 2887 3681 3701 2887
Size: 794 20 4557
Fragments arranged by size:
4557 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5371
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5336
DraI TTT'AAA
Cuts at: 2675 2675
Size: 5371
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2284 4378 809
Size: 507 968 2094 1802
Fragments arranged by size:
2094 1802 968 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2154 2818 3707
Size: 54 1425 50 275 18 324 664 889
Cuts at: 3707 5336 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 664 324 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2818 1537
Size: 275 1006 4090
Fragments arranged by size:
4090 1006 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2469 3301 1353
Size: 1116 832 3423
Fragments arranged by size:
3423 1116 832
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3958 4332 4478 387
Size: 1064 138 2369 374 146 1280
Fragments arranged by size:
2369 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2375 2660 3434 4031 4818 5086 1239
Size: 393 743 285 774 597 787 268 1524
Fragments arranged by size:
1524 787 774 743 597 393 285 268
EcoO109I rG'GnC_Cy
Cuts at: 2064 2065 2064
Size: 1 5370
EcoRI G'AATT_C
Cuts at: 2680 3058 2680
Size: 378 4993
FspI TGC'GCA
Cuts at: 2207 2225 3877 2207
Size: 18 1652 3701
Fragments arranged by size:
3701 1652 18
FspAI rTGC'GCAy
Cuts at: 2207 2207
Size: 5371
HaeII r_GCGC'y
Cuts at: 1678 2026 2218 4518 1678
Size: 348 192 2300 2531
Fragments arranged by size:
2531 2300 348 192
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2525 2557 4012 4044 4086
Size: 32 135 32 953 32 1455 32 42
Cuts at: 4086 4118 1373
Size: 32 2626
Fragments arranged by size:
2626 1455 953 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2715 2961 669
Size: 988 281 777 246 3079
Fragments arranged by size:
3079 988 777 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5371
HpaI GTT'AAC
Cuts at: 2961 2961
Size: 5371
KasI G'GCGC_C
Cuts at: 2022 2022
Size: 5371
KpnI G_GTAC'C
Cuts at: 2696 2696
Size: 5371
MfeI C'AATT_G
Cuts at: 2970 2970
Size: 5371
MluI A'CGCG_T
Cuts at: 5247 5247
Size: 5371
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2313 4485 4669 1305
Size: 1008 2172 184 2007
Fragments arranged by size:
2172 2007 1008 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2156 10
Size: 54 1425 343 324 3225
Fragments arranged by size:
3225 1425 343 324 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2414 3329 3688 3847 518
Size: 648 1248 915 359 159 2042
Fragments arranged by size:
2042 1248 915 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2353 2787 3464 4612 4857 1606
Size: 747 434 677 1148 245 2120
Fragments arranged by size:
2120 1148 747 677 434 245
NarI GG'CG_CC
Cuts at: 2023 2023
Size: 5371
NcoI C'CATG_G
Cuts at: 513 1065 2004 2331 2454 513
Size: 552 939 327 123 3430
Fragments arranged by size:
3430 939 552 327 123
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5371
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5371
NotI GC'GGCC_GC
Cuts at: 2818 2818
Size: 5371
NspI r_CATG'y
Cuts at: 1132 1229 2729 4274 1132
Size: 97 1500 1545 2229
Fragments arranged by size:
2229 1545 1500 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5371
PciI A'CATG_T
Cuts at: 4270 4270
Size: 5371
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2506 1108
Size: 1398 3973
PmeI GTTT'AAAC
Cuts at: 2675 2675
Size: 5371
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2537 2569 3440 3472 4953 4985 2537
Size: 32 871 32 1481 32 2923
Fragments arranged by size:
2923 1481 871 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5371
PsiI TTA'TAA
Cuts at: 2941 2941
Size: 5371
PspOMI G'GGCC_C
Cuts at: 2064 2064
Size: 5371
PstI C_TGCA'G
Cuts at: 830 2723 830
Size: 1893 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5371
PvuII CAG'CTG
Cuts at: 2353 2353
Size: 5371
SalI G'TCGA_C
Cuts at: 1936 2713 1936
Size: 777 4594
SbfI CC_TGCA'GG
Cuts at: 2723 2723
Size: 5371
ScaI AGT'ACT
Cuts at: 3619 5331 3619
Size: 1712 3659
SfcI C'TryA_G
Cuts at: 826 1045 2719 3854 4535 4726 826
Size: 219 1674 1135 681 191 1471
Fragments arranged by size:
1674 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2024 2024
Size: 5371
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5371
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5371
SmaI CCC'GGG
Cuts at: 1872 2698 1872
Size: 826 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2267 2282 2651 3475 4376 4638
Size: 197 926 324 15 369 824 901 262
Cuts at: 4638 820
Size: 1553
Fragments arranged by size:
1553 926 901 824 369 324 262 197
15
SnaBI TAC'GTA
Cuts at: 493 3162 493
Size: 2669 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5371
SphI G_CATG'C
Cuts at: 1132 2729 1132
Size: 1597 3774
SspI AAT'ATT
Cuts at: 5 52 3295 5
Size: 47 3243 2081
Fragments arranged by size:
3243 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5371
StyI C'CwwG_G
Cuts at: 513 1065 2004 2325 2331 2454 2810 513
Size: 552 939 321 6 123 356 3074
Fragments arranged by size:
3074 939 552 356 321 123 6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2447 3398 3415 3568 3753 4198 325
Size: 1255 867 951 17 153 185 445 1498
Fragments arranged by size:
1498 1255 951 867 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3617 5329 96
Size: 274 80 33 51 1443 1640 1712 138
Fragments arranged by size:
1712 1640 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5331 5363 5331
Size: 32 5339
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5371
XbaI T'CTAG_A
Cuts at: 2707 2707
Size: 5371
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2086 2296 2086
Size: 210 5161
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5371
XmaI C'CCGG_G
Cuts at: 1870 2696 1870
Size: 826 4545
XmnI GAAnn'nnTTC
Cuts at: 3500 3500
Size: 5371
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3179 276
Size: 53 83 186 936 1645 2468
Fragments arranged by size:
2468 1645 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI NaeI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI
SexAI SfiI SrfI SwaI