Restriction map for
pFN21AE9653
(Circular) (Six-base) MAPSORT of: pFN21AE9653.seq Check: 4836 from: 1 to: 11731
pFN21AE9653 11731 bp
With 182 enzymes: SgfI *
March 28, 2012 12:08 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 9541 278
Size: 53 83 186 936 8005 2468
Fragments arranged by size:
8005 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4101 5865 9074 1937
Size: 2164 1764 3209 4594
Fragments arranged by size:
4594 3209 2164 1764
Acc65I G'GTAC_C
Cuts at: 9052 9052
Size: 11731
AclI AA'CG_TT
Cuts at: 1563 9858 10231 1563
Size: 8295 373 3063
Fragments arranged by size:
8295 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2004 2090 2691 3138 3891 6623 7242 7497
Size: 372 86 601 447 753 2732 619 255
Cuts at: 7497 7576 7994 8295 8394 8993 9794 11178 11446
Size: 79 418 301 99 599 801 1384 268
Cuts at: 11446 1632
Size: 1917
Fragments arranged by size:
2732 1917 1384 801 753 619 601 599
447 418 372 301 268 255 99 86
79
AflII C'TTAA_G
Cuts at: 820 1017 2864 5285 8138 820
Size: 197 1847 2421 2853 4413
Fragments arranged by size:
4413 2853 2421 1847 197
AflIII A'CryG_T
Cuts at: 1203 4805 8648 10630 11607 1203
Size: 3602 3843 1982 977 1327
Fragments arranged by size:
3843 3602 1982 1327 977
AgeI A'CCGG_T
Cuts at: 10562 10562
Size: 11731
AhdI GACnn_n'nnGTC
Cuts at: 2547 3405 3810 10460 2547
Size: 858 405 6650 3818
Fragments arranged by size:
6650 3818 858 405
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 11731
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 8090 8349 1184
Size: 6906 259 4566
Fragments arranged by size:
6906 4566 259
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3822 3854 3822
Size: 32 11699
AlwNI CAG_nnn'CTG
Cuts at: 3733 6716 9474 11046 3733
Size: 2983 2758 1572 4418
Fragments arranged by size:
4418 2983 2758 1572
ApaI G_GGCC'C
Cuts at: 5162 5162
Size: 11731
ApaLI G'TGCA_C
Cuts at: 8702 9788 10944 8702
Size: 1086 1156 9489
Fragments arranged by size:
9489 1156 1086
ApoI r'AATT_y
Cuts at: 1493 2680 2794 2926 3433 3573 4267 4592 4620
Size: 1187 114 132 507 140 694 325 28
Cuts at: 4620 5645 5819 6520 7986 8185 8687 8991 9040
Size: 1025 174 701 1466 199 502 304 49
Cuts at: 9040 9266 9418 11527 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 1466 1187 1025 701 694 507
502 325 304 226 199 174 152 140
132 114 49 28
AseI AT'TA_AT
Cuts at: 160 1032 8784 10285 160
Size: 872 7752 1501 1606
Fragments arranged by size:
7752 1606 1501 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11731
AvaI C'yCGr_G
Cuts at: 1870 1943 9056 1870
Size: 73 7113 4545
Fragments arranged by size:
7113 4545 73
AvrII C'CTAG_G
Cuts at: 5029 5029
Size: 11731
BamHI G'GATC_C
Cuts at: 5972 6262 9061 9428 5972
Size: 290 2799 367 8275
Fragments arranged by size:
8275 2799 367 290
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2269 4768 9052 10507 618
Size: 325 217 627 482 2499 4284 1455 1842
Fragments arranged by size:
4284 2499 1842 1455 627 482 325 217
BanII G_rGCy'C
Cuts at: 1391 5162 9050 1391
Size: 3771 3888 4072
Fragments arranged by size:
4072 3888 3771
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2010 2071 3483 4686 7800 8026 8651
Size: 971 111 61 1412 1203 3114 226 625
Cuts at: 8651 928
Size: 4008
Fragments arranged by size:
4008 3114 1412 1203 971 625 226 111
61
BbvCI CC'TCA_GC
Cuts at: 2321 6405 2321
Size: 4084 7647
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2298 2332 9922 9956 1903
Size: 34 361 34 7590 34 3678
Fragments arranged by size:
7590 3678 361 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 9623 10833 9623
Size: 1210 10521
BclI T'GATC_A
Cuts at: 1548 7465 8431 1548
Size: 5917 966 4848
Fragments arranged by size:
5917 4848 966
BfrBI ATG'CAT
Cuts at: 2854 6874 2854
Size: 4020 7711
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 10342 136
Size: 107 122 71 631 605 8670 1525
Fragments arranged by size:
8670 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3066 6250 6307 9001 11726 3066
Size: 3184 57 2694 2725 3071
Fragments arranged by size:
3184 3071 2725 2694 57
BlpI GC'TnA_GC
Cuts at: 4281 9148 4281
Size: 4867 6864
Bme1580I G_kGCm'C
Cuts at: 1790 5162 5386 8706 9792 10948 1790
Size: 3372 224 3320 1086 1156 2573
Fragments arranged by size:
3372 3320 2573 1156 1086 224
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 11731
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2412 2929 6077
Size: 416 53 83 399 24 995 517 3148
Cuts at: 6077 9462 10420 442
Size: 3385 958 1753
Fragments arranged by size:
3385 3148 1753 995 958 517 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 11731
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3004 3004
Size: 11731
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5156 5408 6720 6807 6901 7697 7773 8330
Size: 3917 252 1312 87 94 796 76 557
Cuts at: 8330 8978 10391 1239
Size: 648 1413 2579
Fragments arranged by size:
3917 2579 1413 1312 796 648 557 252
94 87 76
Bpu10I CC'TnA_GC
Cuts at: 2321 6405 7523 2321
Size: 4084 1118 6529
Fragments arranged by size:
6529 4084 1118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2644 7678 9856 10721 11019 2644
Size: 5034 2178 865 298 3356
Fragments arranged by size:
5034 3356 2178 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2047 2975 7153 10394 882
Size: 617 548 928 4178 3241 2219
Fragments arranged by size:
4178 3241 2219 928 617 548
BsaAI yAC'GTr
Cuts at: 493 1204 9522 493
Size: 711 8318 2702
Fragments arranged by size:
8318 2702 711
BsaBI GATnn'nnATC
Cuts at: 4068 6249 7125 8568 11725 4068
Size: 2181 876 1443 3157 4074
Fragments arranged by size:
4074 3157 2181 1443 876
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 9538 9920 10555 275
Size: 53 83 186 936 8005 382 635 1451
Fragments arranged by size:
8005 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3518 3865 10164 10562 10836 10983 11394 3518
Size: 347 6299 398 274 147 411 3855
Fragments arranged by size:
6299 3855 411 398 347 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 5129 5159 6899 6929 8720
Size: 30 165 30 3726 30 1740 30 1791
Cuts at: 8720 8750 1178
Size: 30 4159
Fragments arranged by size:
4159 3726 1791 1740 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3440 3539 4041 5078 8297 8568 8741 1186
Size: 2254 99 502 1037 3219 271 173 4176
Fragments arranged by size:
4176 3219 2254 1037 502 271 173 99
BseYI C'CCAG_C
Cuts at: 8658 10934 11430 8658
Size: 2276 496 8959
Fragments arranged by size:
8959 2276 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3597 6221 6513 8096 1163
Size: 573 1861 2624 292 1583 4798
Fragments arranged by size:
4798 2624 1861 1583 573 292
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 9181 9942 10970 1540
Size: 275 186 7180 761 1028 2301
Fragments arranged by size:
7180 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 8706 9792 9877 10948 8706
Size: 1086 85 1071 9489
Fragments arranged by size:
9489 1086 1071 85
BsmI GAATG_Cn'
Cuts at: 2071 3121 9242 9335 2071
Size: 1050 6121 93 4467
Fragments arranged by size:
6121 4467 1050 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 5162 5386 8706 9050 9792 9877 10948
Size: 399 3372 224 3320 344 742 85 1071
Cuts at: 10948 1391
Size: 2174
Fragments arranged by size:
3372 3320 2174 1071 742 399 344 224
85
BspEI T'CCGG_A
Cuts at: 3518 11394 3518
Size: 7876 3855
BspHI T'CATG_A
Cuts at: 4219 9620 11350 4219
Size: 5401 1730 4600
Fragments arranged by size:
5401 4600 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3082 7172 9086 844
Size: 2238 4090 1914 3489
Fragments arranged by size:
4090 3489 2238 1914
BsrBI CCG'CTC
Cuts at: 1394 1823 9618 1394
Size: 429 7795 3507
Fragments arranged by size:
7795 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2271 2414 3034 6928 8707 10226 10400
Size: 1183 1023 143 620 3894 1779 1519 174
Cuts at: 10400 65
Size: 1396
Fragments arranged by size:
3894 1779 1519 1396 1183 1023 620 174
143
BsrFI r'CCGG_y
Cuts at: 1700 1810 7144 10375 10562 1700
Size: 110 5334 3231 187 2869
Fragments arranged by size:
5334 3231 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 4579 5224 5876 8651 96
Size: 4483 645 652 2775 3176
Fragments arranged by size:
4483 3176 2775 652 645
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 11731
BssSI C'ACGA_G
Cuts at: 9791 10803 9791
Size: 1012 10719
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2290 6880 8828 794
Size: 1496 4590 1948 3697
Fragments arranged by size:
4590 3697 1948 1496
BstBI TT'CG_AA
Cuts at: 10535 10535
Size: 11731
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 11731
BstYI r'GATC_y
Cuts at: 1972 3066 4369 5972 6250 6262 6307 6712 7172
Size: 1094 1303 1603 278 12 45 405 460
Cuts at: 7172 9001 9061 9428 9813 9830 11282 11368 11380
Size: 1829 60 367 385 17 1452 86 12
Cuts at: 11380 11726 1972
Size: 346 1977
Fragments arranged by size:
1977 1829 1603 1452 1303 1094 460 405
385 367 346 278 86 60 45 17
12 12
Bsu36I CC'TnA_GG
Cuts at: 5688 5688
Size: 11731
BtgI C'CryG_G
Cuts at: 513 1065 2004 2688 3120 3390 5532 513
Size: 552 939 684 432 270 2142 6712
Fragments arranged by size:
6712 2142 939 684 552 432 270
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 10992
Fragments arranged by size:
10992 683 56
BtsI GCAGTG_nn'
Cuts at: 2266 6233 7954 9247 10041 10061 2266
Size: 3967 1721 1293 794 20 3936
Fragments arranged by size:
3967 3936 1721 1293 794 20
ClaI AT'CG_AT
Cuts at: 1554 8148 1554
Size: 6594 5137
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 11696
DraI TTT'AAA
Cuts at: 2640 3580 6507 7006 8742 9035 2640
Size: 940 2927 499 1736 293 5336
Fragments arranged by size:
5336 2927 1736 940 499 293
DraIII CAC_nnn'GTG
Cuts at: 2937 2937
Size: 11731
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2507 10738 809
Size: 507 1191 8231 1802
Fragments arranged by size:
8231 1802 1191 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4456 9178 10067
Size: 54 1425 50 275 18 2626 4722 889
Cuts at: 10067 11696 8
Size: 1629 43
Fragments arranged by size:
4722 2626 1629 1425 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 9178 1537
Size: 275 7366 4090
Fragments arranged by size:
7366 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2109 2842 3910 4350 7473 7644 8193 8274
Size: 756 733 1068 440 3123 171 549 81
Cuts at: 8274 8370 8481 9661 1353
Size: 96 111 1180 3423
Fragments arranged by size:
3423 3123 1180 1068 756 733 549 440
171 111 96 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 10318 10692 10838 387
Size: 1064 138 8729 374 146 1280
Fragments arranged by size:
8729 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2004 2090 2691 3138 3891 5156 5408
Size: 393 372 86 601 447 753 1265 252
Cuts at: 5408 6623 6720 6807 6901 7242 7497 7576 7697
Size: 1215 97 87 94 341 255 79 121
Cuts at: 7697 7773 7994 8295 8330 8394 8978 8993 9794
Size: 76 221 301 35 64 584 15 801
Cuts at: 9794 10391 11178 11446 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1265 1215 801 787 753 601 597
584 447 393 372 341 301 268 255
252 221 121 97 94 87 86 79
76 64 35 15
EcoNI CCTnn'n_nnAGG
Cuts at: 3078 6444 8723 3078
Size: 3366 2279 6086
Fragments arranged by size:
6086 3366 2279
EcoO109I rG'GnC_Cy
Cuts at: 2110 5158 5159 7530 7578 2110
Size: 3048 1 2371 48 6263
Fragments arranged by size:
6263 3048 2371 48 1
EcoRI G'AATT_C
Cuts at: 4267 8687 9040 9418 4267
Size: 4420 353 378 6580
Fragments arranged by size:
6580 4420 378 353
EcoRV GAT'ATC
Cuts at: 4702 4702
Size: 11731
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 8303 8303
Size: 11731
FspI TGC'GCA
Cuts at: 10237 10237
Size: 11731
HaeII r_GCGC'y
Cuts at: 1678 10878 1678
Size: 9200 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2000 2032 2203 2235 2528
Size: 32 135 32 428 32 171 32 293
Cuts at: 2528 2533 2560 2565 2606 2638 3274 3306 3391
Size: 5 27 5 41 32 636 32 85
Cuts at: 3391 3423 3473 3505 3796 3828 5038 5070 5129
Size: 32 50 32 291 32 1210 32 59
Cuts at: 5129 5161 6138 6170 6247 6279 7435 7450 7467
Size: 32 977 32 77 32 1156 15 17
Cuts at: 7467 7482 7565 7597 7792 7824 8320 8352 8416
Size: 15 83 32 195 32 496 32 64
Cuts at: 8416 8448 10372 10404 10446 10478 1373
Size: 32 1924 32 42 32 2626
Fragments arranged by size:
2626 1924 1210 1156 977 636 496 428
293 291 195 171 135 85 83 77
64 59 50 42 41 32 32 32
32 32 32 32 32 32 32 32
32 32 32 32 32 32 32 32
27 17 15 15 5 5
HincII GTy'rAC
Cuts at: 669 1657 1938 9075 9321 669
Size: 988 281 7137 246 3079
Fragments arranged by size:
7137 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2642 3150 6353 7886 8603 748
Size: 1894 508 3203 1533 717 3876
Fragments arranged by size:
3876 3203 1894 1533 717 508
HpaI GTT'AAC
Cuts at: 9321 9321
Size: 11731
KpnI G_GTAC'C
Cuts at: 9056 9056
Size: 11731
MfeI C'AATT_G
Cuts at: 9330 9330
Size: 11731
MluI A'CGCG_T
Cuts at: 11607 11607
Size: 11731
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 10845 11029 1305
Size: 9540 184 2007
Fragments arranged by size:
9540 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4458 10
Size: 54 1425 343 2626 7283
Fragments arranged by size:
7283 2626 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2478 2496 2558 3395 5331 6535 6693
Size: 648 1312 18 62 837 1936 1204 158
Cuts at: 6693 8165 9689 10048 10207 518
Size: 1472 1524 359 159 2042
Fragments arranged by size:
2042 1936 1524 1472 1312 1204 837 648
359 159 158 62 18
MspA1I CmG'CkG
Cuts at: 1606 2281 3611 4061 8266 9147 9824 10972 11217
Size: 675 1330 450 4205 881 677 1148 245
Cuts at: 11217 1606
Size: 2120
Fragments arranged by size:
4205 2120 1330 1148 881 677 675 450
245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3120 3390 5532 513
Size: 552 939 1116 270 2142 6712
Fragments arranged by size:
6712 2142 1116 939 552 270
NdeI CA'TA_TG
Cuts at: 387 2223 6536 7327 387
Size: 1836 4313 791 4791
Fragments arranged by size:
4791 4313 1836 791
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 11731
NotI GC'GGCC_GC
Cuts at: 9178 9178
Size: 11731
NsiI A_TGCA'T
Cuts at: 2856 6876 2856
Size: 4020 7711
NspI r_CATG'y
Cuts at: 1132 1229 2495 2858 4809 8652 9089 10634 1132
Size: 97 1266 363 1951 3843 437 1545 2229
Fragments arranged by size:
3843 2229 1951 1545 1266 437 363 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 11731
PciI A'CATG_T
Cuts at: 4805 8648 10630 4805
Size: 3843 1982 5906
Fragments arranged by size:
5906 3843 1982
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5087 1108
Size: 3979 7752
PfoI T'CCnGG_A
Cuts at: 5080 5233 8511 8636 5080
Size: 153 3278 125 8175
Fragments arranged by size:
8175 3278 153 125
PmeI GTTT'AAAC
Cuts at: 9035 9035
Size: 11731
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2819 2851 9800 9832 11313 11345 2819
Size: 32 6949 32 1481 32 3205
Fragments arranged by size:
6949 3205 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2110 7530 7578 2110
Size: 5420 48 6263
Fragments arranged by size:
6263 5420 48
PshAI GACnn'nnGTC
Cuts at: 1613 8030 1613
Size: 6417 5314
PsiI TTA'TAA
Cuts at: 3855 6230 8763 9301 3855
Size: 2375 2533 538 6285
Fragments arranged by size:
6285 2533 2375 538
PspOMI G'GGCC_C
Cuts at: 5158 5158
Size: 11731
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 8298 8330 8298
Size: 32 11699
PstI C_TGCA'G
Cuts at: 830 2261 3223 6586 8248 9083 830
Size: 1431 962 3363 1662 835 3478
Fragments arranged by size:
3478 3363 1662 1431 962 835
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 11731
PvuII CAG'CTG
Cuts at: 2281 3611 4061 8266 2281
Size: 1330 450 4205 5746
Fragments arranged by size:
5746 4205 1330 450
SalI G'TCGA_C
Cuts at: 1936 9073 1936
Size: 7137 4594
SapI GCTCTTCn'nnn_
Cuts at: 3910 4350 8370 3910
Size: 440 4020 7271
Fragments arranged by size:
7271 4020 440
SbfI CC_TGCA'GG
Cuts at: 9083 9083
Size: 11731
ScaI AGT'ACT
Cuts at: 9979 11691 9979
Size: 1712 10019
SfcI C'TryA_G
Cuts at: 826 1045 2257 3219 4908 5163 5736 6582 6888
Size: 219 1212 962 1689 255 573 846 306
Cuts at: 6888 8244 9079 10214 10895 11086 826
Size: 1356 835 1135 681 191 1471
Fragments arranged by size:
1689 1471 1356 1212 1135 962 846 835
681 573 306 255 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 11731
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 11731
SmaI CCC'GGG
Cuts at: 1872 9058 1872
Size: 7186 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2659 2864 5285 7657 8138 9835
Size: 197 926 716 205 2421 2372 481 1697
Cuts at: 9835 10736 10998 820
Size: 901 262 1553
Fragments arranged by size:
2421 2372 1697 1553 926 901 716 481
262 205 197
SnaBI TAC'GTA
Cuts at: 493 9522 493
Size: 9029 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 11731
SphI G_CATG'C
Cuts at: 1132 9089 1132
Size: 7957 3774
SspI AAT'ATT
Cuts at: 5 52 2082 2890 2953 3306 6128 6511 6613
Size: 47 2030 808 63 353 2822 383 102
Cuts at: 6613 6904 9655 5
Size: 291 2751 2081
Fragments arranged by size:
2822 2751 2081 2030 808 383 353 291
102 63 47
StuI AGG'CCT
Cuts at: 1517 2574 4894 5873 7292 7979 1517
Size: 1057 2320 979 1419 687 5269
Fragments arranged by size:
5269 2320 1419 1057 979 687
StyI C'CwwG_G
Cuts at: 513 1065 2004 2093 2817 3120 3390 5029 5532
Size: 552 939 89 724 303 270 1639 503
Cuts at: 5532 8044 8719 9170 513
Size: 2512 675 451 3074
Fragments arranged by size:
3074 2512 1639 939 724 675 552 503
451 303 270 89
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2948 5663 6547 9758 9775 9928 10113
Size: 1255 1368 2715 884 3211 17 153 185
Cuts at: 10113 10558 325
Size: 445 1498
Fragments arranged by size:
3211 2715 1498 1368 1255 884 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4255 4579 5051
Size: 274 80 33 51 1443 2278 324 472
Cuts at: 5051 5224 5876 6014 8651 9977 11689 96
Size: 173 652 138 2637 1326 1712 138
Fragments arranged by size:
2637 2278 1712 1443 1326 652 472 324
274 173 138 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 11691 11723 11691
Size: 32 11699
Tth111I GACn'n_nGTC
Cuts at: 1316 2215 4948 1316
Size: 899 2733 8099
Fragments arranged by size:
8099 2733 899
XbaI T'CTAG_A
Cuts at: 6640 9067 6640
Size: 2427 9304
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 11731
XmaI C'CCGG_G
Cuts at: 1870 9056 1870
Size: 7186 4545
XmnI GAAnn'nnTTC
Cuts at: 2133 2352 3948 6192 6612 7101 7227 7392 9860
Size: 219 1596 2244 420 489 126 165 2468
Cuts at: 9860 2133
Size: 4004
Fragments arranged by size:
4004 2468 2244 1596 489 420 219 165
126
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 9539 276
Size: 53 83 186 936 8005 2468
Fragments arranged by size:
8005 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PsrI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI BaeI BbeI BsiWI BsmBI BstXI
BstZ17I EcoICRI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PmlI PspXI RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI