Restriction map for
pFN21AE9647
(Circular) (Six-base) MAPSORT of: pFN21AE9647.seq Check: 118 from: 1 to: 9106
pFN21AE9647 9106 bp
With 182 enzymes: SgfI *
March 28, 2012 12:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 5797 5797
Size: 9106
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2967 3627 6916 278
Size: 53 83 186 936 1431 660 3289 2468
Fragments arranged by size:
3289 2468 1431 936 660 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3262 3776 4554 6449 1937
Size: 1325 514 778 1895 4594
Fragments arranged by size:
4594 1895 1325 778 514
Acc65I G'GTAC_C
Cuts at: 3480 3639 4304 6427 3480
Size: 159 665 2123 6159
Fragments arranged by size:
6159 2123 665 159
AclI AA'CG_TT
Cuts at: 1563 7233 7606 1563
Size: 5670 373 3063
Fragments arranged by size:
5670 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2232 3008 3087 3114 3260 3461 3725 3884
Size: 600 776 79 27 146 201 264 159
Cuts at: 3884 4829 4999 5409 5525 7169 8553 8821 1632
Size: 945 170 410 116 1644 1384 268 1917
Fragments arranged by size:
1917 1644 1384 945 776 600 410 268
264 201 170 159 146 116 79 27
AflII C'TTAA_G
Cuts at: 820 1017 3839 5755 820
Size: 197 2822 1916 4171
Fragments arranged by size:
4171 2822 1916 197
AflIII A'CryG_T
Cuts at: 1203 3048 3570 6261 8005 8982 1203
Size: 1845 522 2691 1744 977 1327
Fragments arranged by size:
2691 1845 1744 1327 977 522
AgeI A'CCGG_T
Cuts at: 7937 7937
Size: 9106
AhdI GACnn_n'nnGTC
Cuts at: 7835 7835
Size: 9106
AleI CACnn'nnGTG
Cuts at: 1166 4327 1166
Size: 3161 5945
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 9106
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2270 2302 3570 3602 2270
Size: 32 1268 32 7774
Fragments arranged by size:
7774 1268 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3439 4996 5876 6092 6849 8421 3439
Size: 1557 880 216 757 1572 4124
Fragments arranged by size:
4124 1572 1557 880 757 216
ApaLI G'TGCA_C
Cuts at: 2215 7163 8319 2215
Size: 4948 1156 3002
Fragments arranged by size:
4948 3002 1156
ApoI r'AATT_y
Cuts at: 1493 4268 6415 6641 6793 8902 1493
Size: 2775 2147 226 152 2109 1697
Fragments arranged by size:
2775 2147 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4398 5280 7660 160
Size: 872 3366 882 2380 1606
Fragments arranged by size:
3366 2380 1606 882 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9106
AvaI C'yCGr_G
Cuts at: 1870 1943 2529 5568 5651 6431 1870
Size: 73 586 3039 83 780 4545
Fragments arranged by size:
4545 3039 780 586 83 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 5317 5350 5849 5882 5317
Size: 33 499 33 8541
Fragments arranged by size:
8541 499 33 33
BamHI G'GATC_C
Cuts at: 2838 6436 6803 2838
Size: 3598 367 5141
Fragments arranged by size:
5141 3598 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2262 2699 3480 3548 3639
Size: 325 217 627 475 437 781 68 91
Cuts at: 3639 4304 4484 5739 6046 6310 6427 7882 618
Size: 665 180 1255 307 264 117 1455 1842
Fragments arranged by size:
1842 1455 1255 781 665 627 475 437
325 307 264 217 180 117 91 68
BanII G_rGCy'C
Cuts at: 1391 2314 2621 5588 6425 1391
Size: 923 307 2967 837 4072
Fragments arranged by size:
4072 2967 923 837 307
BbeI G_GCGC'C
Cuts at: 2266 2266
Size: 9106
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2136 2779 3656 4932 928
Size: 971 237 643 877 1276 5102
Fragments arranged by size:
5102 1276 971 877 643 237
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3317 3351 7297 7331 1903
Size: 34 1380 34 3946 34 3678
Fragments arranged by size:
3946 3678 1380 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5877 6129 6998 8208 5877
Size: 252 869 1210 6775
Fragments arranged by size:
6775 1210 869 252
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9106
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2271 3077 3369
Size: 107 122 71 631 605 599 806 292
Cuts at: 3369 7717 136
Size: 4348 1525
Fragments arranged by size:
4348 1525 806 631 605 599 292 122
107 71
BglII A'GATC_T
Cuts at: 3864 4623 9101 3864
Size: 759 4478 3869
Fragments arranged by size:
4478 3869 759
BlpI GC'TnA_GC
Cuts at: 6523 6523
Size: 9106
Bme1580I G_kGCm'C
Cuts at: 1790 2219 3970 7167 8323 1790
Size: 429 1751 3197 1156 2573
Fragments arranged by size:
3197 2573 1751 1156 429
BmgBI CAC'GTC
Cuts at: 1318 2053 3937 1318
Size: 735 1884 6487
Fragments arranged by size:
6487 1884 735
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2176 3287 3486
Size: 416 53 83 399 24 759 1111 199
Cuts at: 3486 5738 6408 6837 7795 442
Size: 2252 670 429 958 1753
Fragments arranged by size:
2252 1753 1111 958 759 670 429 416
399 199 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9106
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2081 2444 2492 2643 2754 2894 3689 4167
Size: 842 363 48 151 111 140 795 478
Cuts at: 4167 5131 7766 1239
Size: 964 2635 2579
Fragments arranged by size:
2635 2579 964 842 795 478 363 151
140 111 48
Bpu10I CC'TnA_GC
Cuts at: 2258 2258
Size: 9106
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 7231 8096 8394 7231
Size: 865 298 7943
Fragments arranged by size:
7943 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 7769 882
Size: 617 6270 2219
Fragments arranged by size:
6270 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 6897 493
Size: 711 5693 2702
Fragments arranged by size:
5693 2702 711
BsaBI GATnn'nnATC
Cuts at: 4680 9100 4680
Size: 4420 4686
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2263 2733 2964 3624
Size: 53 83 186 936 730 470 231 660
Cuts at: 3624 6913 7295 7930 275
Size: 3289 382 635 1451
Fragments arranged by size:
3289 1451 936 730 660 635 470 382
231 186 83 53
BsaWI w'CCGG_w
Cuts at: 2841 3642 7539 7937 8211 8358 8769 2841
Size: 801 3897 398 274 147 411 3178
Fragments arranged by size:
3897 3178 801 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2270 2300 5118 5148 5190
Size: 30 165 30 867 30 2818 30 42
Cuts at: 5190 5220 6156 6186 1178
Size: 30 936 30 4098
Fragments arranged by size:
4098 2818 936 867 165 42 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2090 2093 2096 2099 2102 2105 2120 2517
Size: 904 3 3 3 3 3 15 397
Cuts at: 2517 2642 2789 3221 3700 1186
Size: 125 147 432 479 6592
Fragments arranged by size:
6592 904 479 432 397 147 125 15
3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 3823 4415 5942 6143 6221 6283 8309 8805 3823
Size: 592 1527 201 78 62 2026 496 4124
Fragments arranged by size:
4124 2026 1527 592 496 201 78 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2624 3641 3701 4709 5102 5214 5228
Size: 573 888 1017 60 1008 393 112 14
Cuts at: 5228 5867 1163
Size: 639 4402
Fragments arranged by size:
4402 1017 1008 888 639 573 393 112
60 14
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6556 7317 8345 1540
Size: 275 186 4555 761 1028 2301
Fragments arranged by size:
4555 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2219 2314 2621 3144 4324 5588 7167 7252 8323
Size: 95 307 523 1180 1264 1579 85 1071
Cuts at: 8323 2219
Size: 3002
Fragments arranged by size:
3002 1579 1264 1180 1071 523 307 95
85
BsmI GAATG_Cn'
Cuts at: 3845 6617 6710 3845
Size: 2772 93 6241
Fragments arranged by size:
6241 2772 93
BsmBI CGTCTCn'nnnn_
Cuts at: 5512 5905 5512
Size: 393 8713
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2219 2314 2621 3144 3970 4324 5588
Size: 399 429 95 307 523 826 354 1264
Cuts at: 5588 6425 7167 7252 8323 1391
Size: 837 742 85 1071 2174
Fragments arranged by size:
2174 1264 1071 837 826 742 523 429
399 354 307 95 85
BspEI T'CCGG_A
Cuts at: 8769 8769
Size: 9106
BspHI T'CATG_A
Cuts at: 6328 6995 8725 6328
Size: 667 1730 6709
Fragments arranged by size:
6709 1730 667
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2142 5797 6461 844
Size: 1298 3655 664 3489
Fragments arranged by size:
3655 3489 1298 664
BsrBI CCG'CTC
Cuts at: 1394 1823 3453 4390 6993 1394
Size: 429 1630 937 2603 3507
Fragments arranged by size:
3507 2603 1630 937 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3605 4853 5689 7601 7775 65
Size: 1183 2357 1248 836 1912 174 1396
Fragments arranged by size:
2357 1912 1396 1248 1183 836 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3010 5811 7750 7937 1700
Size: 110 1200 2801 1939 187 2869
Fragments arranged by size:
2869 2801 1939 1200 187 110
BsrGI T'GTAC_A
Cuts at: 96 3222 5266 96
Size: 3126 2044 3936
Fragments arranged by size:
3936 3126 2044
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9106
BssSI C'ACGA_G
Cuts at: 3159 7166 8178 3159
Size: 4007 1012 4087
Fragments arranged by size:
4087 4007 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3161 5746 794
Size: 2367 2585 4154
Fragments arranged by size:
4154 2585 2367
BstBI TT'CG_AA
Cuts at: 4336 7910 4336
Size: 3574 5532
BstEII G'GTnAC_C
Cuts at: 1184 5218 1184
Size: 4034 5072
BstXI CCAn_nnnn'nTGG
Cuts at: 4495 5729 6001 4495
Size: 1234 272 7600
Fragments arranged by size:
7600 1234 272
BstYI r'GATC_y
Cuts at: 1972 2838 3759 3864 4221 4623 5349 6436 6803
Size: 866 921 105 357 402 726 1087 367
Cuts at: 6803 7188 7205 8657 8743 8755 9101 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1087 921 866 726 402 385
367 357 346 105 86 17 12
BstZ17I GTA'TAC
Cuts at: 3777 4555 3777
Size: 778 8328
Bsu36I CC'TnA_GG
Cuts at: 2023 2484 3618 5410 5487 2023
Size: 461 1134 1792 77 5642
Fragments arranged by size:
5642 1792 1134 461 77
BtgI C'CryG_G
Cuts at: 513 1065 2210 5308 6075 513
Size: 552 1145 3098 767 3544
Fragments arranged by size:
3544 3098 1145 767 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8367
Fragments arranged by size:
8367 683 56
BtsI GCAGTG_nn'
Cuts at: 2106 2575 2581 2662 2899 5647 6090 6622 7416
Size: 469 6 81 237 2748 443 532 794
Cuts at: 7416 7436 2106
Size: 20 3776
Fragments arranged by size:
3776 2748 794 532 469 443 237 81
20 6
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9106
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9071
DraI TTT'AAA
Cuts at: 3118 6410 3118
Size: 3292 5814
DraIII CAC_nnn'GTG
Cuts at: 2647 3700 5746 2647
Size: 1053 2046 6007
Fragments arranged by size:
6007 2046 1053
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5685 8113 809
Size: 507 4369 2428 1802
Fragments arranged by size:
4369 2428 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3915 4564 5064
Size: 54 1425 50 275 18 2085 649 500
Cuts at: 5064 5496 5719 5778 6553 7442 9071 8
Size: 432 223 59 775 889 1629 43
Fragments arranged by size:
2085 1629 1425 889 775 649 500 432
275 223 59 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 6553 1537
Size: 275 4741 4090
Fragments arranged by size:
4741 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2067 2300 2394 2682 2975 3027 6076 7036
Size: 714 233 94 288 293 52 3049 960
Cuts at: 7036 1353
Size: 3423
Fragments arranged by size:
3423 3049 960 714 293 288 233 94
52
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2963 3617 7693 8067 8213 387
Size: 1064 138 1374 654 4076 374 146 1280
Fragments arranged by size:
4076 1374 1280 1064 654 374 146 138
EcoICRI GAG'CTC
Cuts at: 2312 2619 5586 2312
Size: 307 2967 5832
Fragments arranged by size:
5832 2967 307
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2081 2232 2444 2492 2643 2754 2894
Size: 393 449 151 212 48 151 111 140
Cuts at: 2894 3008 3087 3114 3260 3461 3689 3725 3884
Size: 114 79 27 146 201 228 36 159
Cuts at: 3884 4167 4829 4999 5131 5409 5525 7169 7766
Size: 283 662 170 132 278 116 1644 597
Cuts at: 7766 8553 8821 1239
Size: 787 268 1524
Fragments arranged by size:
1644 1524 787 662 597 449 393 283
278 268 228 212 201 170 159 151
151 146 140 132 116 114 111 79
48 36 27
EcoNI CCTnn'n_nnAGG
Cuts at: 2483 3902 4193 4381 5679 2483
Size: 1419 291 188 1298 5910
Fragments arranged by size:
5910 1419 1298 291 188
EcoO109I rG'GnC_Cy
Cuts at: 2293 2746 3928 4123 5423 6056 2293
Size: 453 1182 195 1300 633 5343
Fragments arranged by size:
5343 1300 1182 633 453 195
EcoRI G'AATT_C
Cuts at: 4268 6415 6793 4268
Size: 2147 378 6581
Fragments arranged by size:
6581 2147 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3928 3928
Size: 9106
FspI TGC'GCA
Cuts at: 4968 7612 4968
Size: 2644 6462
HaeII r_GCGC'y
Cuts at: 1678 2266 2422 8253 1678
Size: 588 156 5831 2531
Fragments arranged by size:
5831 2531 588 156
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2132 2164 3142 3170 3174
Size: 32 135 32 560 32 978 28 4
Cuts at: 3174 3202 3390 3422 3856 3888 4835 4867 7747
Size: 28 188 32 434 32 947 32 2880
Cuts at: 7747 7779 7821 7853 1373
Size: 32 42 32 2626
Fragments arranged by size:
2880 2626 978 947 560 434 188 135
42 32 32 32 32 32 32 32
32 28 28 4
HincII GTy'rAC
Cuts at: 669 1657 1938 2755 6450 6696 669
Size: 988 281 817 3695 246 3079
Fragments arranged by size:
3695 3079 988 817 281 246
HindIII A'AGCT_T
Cuts at: 748 4208 748
Size: 3460 5646
HpaI GTT'AAC
Cuts at: 6696 6696
Size: 9106
KasI G'GCGC_C
Cuts at: 2262 2262
Size: 9106
KpnI G_GTAC'C
Cuts at: 3484 3643 4308 6431 3484
Size: 159 665 2123 6159
Fragments arranged by size:
6159 2123 665 159
MfeI C'AATT_G
Cuts at: 2366 6705 2366
Size: 4339 4767
MluI A'CGCG_T
Cuts at: 8982 8982
Size: 9106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2385 5378 8220 8404 1305
Size: 1080 2993 2842 184 2007
Fragments arranged by size:
2993 2842 2007 1080 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3917 4566 5066 5498 5721
Size: 54 1425 343 2085 649 500 432 223
Cuts at: 5721 5780 10
Size: 59 3336
Fragments arranged by size:
3336 2085 1425 649 500 432 343 223
59 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2709 3879 4111 4327 5099 7064 7423
Size: 648 1543 1170 232 216 772 1965 359
Cuts at: 7423 7582 518
Size: 159 2042
Fragments arranged by size:
2042 1965 1543 1170 772 648 359 232
216 159
MspA1I CmG'CkG
Cuts at: 1606 2212 2470 3816 3972 5989 6287 6522 7199
Size: 606 258 1346 156 2017 298 235 677
Cuts at: 7199 8347 8592 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 2017 1346 1148 677 606 298 258
245 235 156
NaeI GCC'GGC
Cuts at: 5813 5813
Size: 9106
NarI GG'CG_CC
Cuts at: 2263 2263
Size: 9106
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 8554
NdeI CA'TA_TG
Cuts at: 387 4108 387
Size: 3721 5385
NgoMIV G'CCGG_C
Cuts at: 5811 5811
Size: 9106
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9106
NotI GC'GGCC_GC
Cuts at: 6553 6553
Size: 9106
NspI r_CATG'y
Cuts at: 1132 1229 2164 2180 3052 3574 4657 5117 6244
Size: 97 935 16 872 522 1083 460 1127
Cuts at: 6244 6265 6275 6464 8009 1132
Size: 21 10 189 1545 2229
Fragments arranged by size:
2229 1545 1127 1083 935 872 522 460
189 97 21 16 10
PasI CC'CwG_GG
Cuts at: 1348 5419 1348
Size: 4071 5035
PciI A'CATG_T
Cuts at: 3048 3570 6261 8005 3048
Size: 522 2691 1744 4149
Fragments arranged by size:
4149 2691 1744 522
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2923 5632 5876 6290 1108
Size: 1815 2709 244 414 3924
Fragments arranged by size:
3924 2709 1815 414 244
PfoI T'CCnGG_A
Cuts at: 2355 3798 4080 4514 5242 5819 2355
Size: 1443 282 434 728 577 5642
Fragments arranged by size:
5642 1443 728 577 434 282
PmeI GTTT'AAAC
Cuts at: 6410 6410
Size: 9106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2270 2302 5659 5691 6332 6364 7175 7207 8688
Size: 32 3357 32 641 32 811 32 1481
Cuts at: 8688 8720 2270
Size: 32 2656
Fragments arranged by size:
3357 2656 1481 811 641 32 32 32
32 32
PpuMI rG'GwC_Cy
Cuts at: 2293 2746 3928 4123 5423 2293
Size: 453 1182 195 1300 5976
Fragments arranged by size:
5976 1300 1182 453 195
PshAI GACnn'nnGTC
Cuts at: 1613 4148 1613
Size: 2535 6571
PsiI TTA'TAA
Cuts at: 6676 6676
Size: 9106
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3767 3799 3767
Size: 32 9074
PstI C_TGCA'G
Cuts at: 830 3958 6458 830
Size: 3128 2500 3478
Fragments arranged by size:
3478 3128 2500
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9106
PvuII CAG'CTG
Cuts at: 5989 6287 5989
Size: 298 8808
SacI G_AGCT'C
Cuts at: 2314 2621 5588 2314
Size: 307 2967 5832
Fragments arranged by size:
5832 2967 307
SacII CC_GC'GG
Cuts at: 2213 2213
Size: 9106
SalI G'TCGA_C
Cuts at: 1936 6448 1936
Size: 4512 4594
SbfI CC_TGCA'GG
Cuts at: 6458 6458
Size: 9106
ScaI AGT'ACT
Cuts at: 3509 7354 9066 3509
Size: 3845 1712 3549
Fragments arranged by size:
3845 3549 1712
SexAI A'CCwGG_T
Cuts at: 4300 4300
Size: 9106
SfcI C'TryA_G
Cuts at: 826 1045 3954 4055 6454 7589 8270 8461 826
Size: 219 2909 101 2399 1135 681 191 1471
Fragments arranged by size:
2909 2399 1471 1135 681 219 191 101
SfoI GGC'GCC
Cuts at: 2264 2264
Size: 9106
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9106
SgrAI Cr'CCGG_yG
Cuts at: 1700 5811 1700
Size: 4111 4995
SmaI CCC'GGG
Cuts at: 1872 5570 5653 6433 1872
Size: 3698 83 780 4545
Fragments arranged by size:
4545 3698 780 83
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3839 5755 7210 8111 8373 820
Size: 197 926 1896 1916 1455 901 262 1553
Fragments arranged by size:
1916 1896 1553 1455 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 6897 493
Size: 6404 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9106
SphI G_CATG'C
Cuts at: 1132 2180 6464 1132
Size: 1048 4284 3774
Fragments arranged by size:
4284 3774 1048
SspI AAT'ATT
Cuts at: 5 52 7030 5
Size: 47 6978 2081
Fragments arranged by size:
6978 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9106
StyI C'CwwG_G
Cuts at: 513 1065 2269 2737 3544 3723 4014 4119 4251
Size: 552 1204 468 807 179 291 105 132
Cuts at: 4251 4273 4830 4887 6545 513
Size: 22 557 57 1658 3074
Fragments arranged by size:
3074 1658 1204 807 557 552 468 291
179 132 105 57 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2717 3172 3280 3306 4457 5485 5757
Size: 1255 1137 455 108 26 1151 1028 272
Cuts at: 5757 7133 7150 7303 7488 7933 325
Size: 1376 17 153 185 445 1498
Fragments arranged by size:
1498 1376 1255 1151 1137 1028 455 445
272 185 153 108 26 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3222 3507 5266
Size: 274 80 33 51 1443 1245 285 1759
Cuts at: 5266 7352 9064 96
Size: 2086 1712 138
Fragments arranged by size:
2086 1759 1712 1443 1245 285 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2147 2179 5190 5222 6156 6188 9066 9098 2147
Size: 32 3011 32 934 32 2878 32 2155
Fragments arranged by size:
3011 2878 2155 934 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 9106
XbaI T'CTAG_A
Cuts at: 3307 4612 6442 3307
Size: 1305 1830 5971
Fragments arranged by size:
5971 1830 1305
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4495 5306 4495
Size: 811 8295
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9106
XmaI C'CCGG_G
Cuts at: 1870 5568 5651 6431 1870
Size: 3698 83 780 4545
Fragments arranged by size:
4545 3698 780 83
XmnI GAAnn'nnTTC
Cuts at: 3897 7235 3897
Size: 3338 5768
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2965 3625 6914 276
Size: 53 83 186 936 1431 660 3289 2468
Fragments arranged by size:
3289 2468 1431 936 660 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI
PvuII SacI SacII SalI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BbvCI BfrBI BplI BsiWI
EcoRV FseI FspAI NruI NsiI PacI PmlI PspOMI
PspXI RsrII SanDI SapI SfiI SrfI SwaI