(Circular) (Six-base) MAPSORT of: pFN21AE9647.seq Check: 118 from: 1 to: 9106 pFN21AE9647 9106 bp With 182 enzymes: SgfI * March 28, 2012 12:19 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5797 5797 Size: 9106 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2967 3627 6916 278 Size: 53 83 186 936 1431 660 3289 2468 Fragments arranged by size: 3289 2468 1431 936 660 186 83 53 AccI GT'mk_AC Cuts at: 1937 3262 3776 4554 6449 1937 Size: 1325 514 778 1895 4594 Fragments arranged by size: 4594 1895 1325 778 514 Acc65I G'GTAC_C Cuts at: 3480 3639 4304 6427 3480 Size: 159 665 2123 6159 Fragments arranged by size: 6159 2123 665 159 AclI AA'CG_TT Cuts at: 1563 7233 7606 1563 Size: 5670 373 3063 Fragments arranged by size: 5670 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2232 3008 3087 3114 3260 3461 3725 3884 Size: 600 776 79 27 146 201 264 159 Cuts at: 3884 4829 4999 5409 5525 7169 8553 8821 1632 Size: 945 170 410 116 1644 1384 268 1917 Fragments arranged by size: 1917 1644 1384 945 776 600 410 268 264 201 170 159 146 116 79 27 AflII C'TTAA_G Cuts at: 820 1017 3839 5755 820 Size: 197 2822 1916 4171 Fragments arranged by size: 4171 2822 1916 197 AflIII A'CryG_T Cuts at: 1203 3048 3570 6261 8005 8982 1203 Size: 1845 522 2691 1744 977 1327 Fragments arranged by size: 2691 1845 1744 1327 977 522 AgeI A'CCGG_T Cuts at: 7937 7937 Size: 9106 AhdI GACnn_n'nnGTC Cuts at: 7835 7835 Size: 9106 AleI CACnn'nnGTG Cuts at: 1166 4327 1166 Size: 3161 5945 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9106 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2270 2302 3570 3602 2270 Size: 32 1268 32 7774 Fragments arranged by size: 7774 1268 32 32 AlwNI CAG_nnn'CTG Cuts at: 3439 4996 5876 6092 6849 8421 3439 Size: 1557 880 216 757 1572 4124 Fragments arranged by size: 4124 1572 1557 880 757 216 ApaLI G'TGCA_C Cuts at: 2215 7163 8319 2215 Size: 4948 1156 3002 Fragments arranged by size: 4948 3002 1156 ApoI r'AATT_y Cuts at: 1493 4268 6415 6641 6793 8902 1493 Size: 2775 2147 226 152 2109 1697 Fragments arranged by size: 2775 2147 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4398 5280 7660 160 Size: 872 3366 882 2380 1606 Fragments arranged by size: 3366 2380 1606 882 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9106 AvaI C'yCGr_G Cuts at: 1870 1943 2529 5568 5651 6431 1870 Size: 73 586 3039 83 780 4545 Fragments arranged by size: 4545 3039 780 586 83 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 5317 5350 5849 5882 5317 Size: 33 499 33 8541 Fragments arranged by size: 8541 499 33 33 BamHI G'GATC_C Cuts at: 2838 6436 6803 2838 Size: 3598 367 5141 Fragments arranged by size: 5141 3598 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2262 2699 3480 3548 3639 Size: 325 217 627 475 437 781 68 91 Cuts at: 3639 4304 4484 5739 6046 6310 6427 7882 618 Size: 665 180 1255 307 264 117 1455 1842 Fragments arranged by size: 1842 1455 1255 781 665 627 475 437 325 307 264 217 180 117 91 68 BanII G_rGCy'C Cuts at: 1391 2314 2621 5588 6425 1391 Size: 923 307 2967 837 4072 Fragments arranged by size: 4072 2967 923 837 307 BbeI G_GCGC'C Cuts at: 2266 2266 Size: 9106 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2136 2779 3656 4932 928 Size: 971 237 643 877 1276 5102 Fragments arranged by size: 5102 1276 971 877 643 237 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3317 3351 7297 7331 1903 Size: 34 1380 34 3946 34 3678 Fragments arranged by size: 3946 3678 1380 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5877 6129 6998 8208 5877 Size: 252 869 1210 6775 Fragments arranged by size: 6775 1210 869 252 BclI T'GATC_A Cuts at: 1548 1548 Size: 9106 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2271 3077 3369 Size: 107 122 71 631 605 599 806 292 Cuts at: 3369 7717 136 Size: 4348 1525 Fragments arranged by size: 4348 1525 806 631 605 599 292 122 107 71 BglII A'GATC_T Cuts at: 3864 4623 9101 3864 Size: 759 4478 3869 Fragments arranged by size: 4478 3869 759 BlpI GC'TnA_GC Cuts at: 6523 6523 Size: 9106 Bme1580I G_kGCm'C Cuts at: 1790 2219 3970 7167 8323 1790 Size: 429 1751 3197 1156 2573 Fragments arranged by size: 3197 2573 1751 1156 429 BmgBI CAC'GTC Cuts at: 1318 2053 3937 1318 Size: 735 1884 6487 Fragments arranged by size: 6487 1884 735 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2176 3287 3486 Size: 416 53 83 399 24 759 1111 199 Cuts at: 3486 5738 6408 6837 7795 442 Size: 2252 670 429 958 1753 Fragments arranged by size: 2252 1753 1111 958 759 670 429 416 399 199 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9106 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2081 2444 2492 2643 2754 2894 3689 4167 Size: 842 363 48 151 111 140 795 478 Cuts at: 4167 5131 7766 1239 Size: 964 2635 2579 Fragments arranged by size: 2635 2579 964 842 795 478 363 151 140 111 48 Bpu10I CC'TnA_GC Cuts at: 2258 2258 Size: 9106 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 7231 8096 8394 7231 Size: 865 298 7943 Fragments arranged by size: 7943 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 7769 882 Size: 617 6270 2219 Fragments arranged by size: 6270 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 6897 493 Size: 711 5693 2702 Fragments arranged by size: 5693 2702 711 BsaBI GATnn'nnATC Cuts at: 4680 9100 4680 Size: 4420 4686 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2263 2733 2964 3624 Size: 53 83 186 936 730 470 231 660 Cuts at: 3624 6913 7295 7930 275 Size: 3289 382 635 1451 Fragments arranged by size: 3289 1451 936 730 660 635 470 382 231 186 83 53 BsaWI w'CCGG_w Cuts at: 2841 3642 7539 7937 8211 8358 8769 2841 Size: 801 3897 398 274 147 411 3178 Fragments arranged by size: 3897 3178 801 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2270 2300 5118 5148 5190 Size: 30 165 30 867 30 2818 30 42 Cuts at: 5190 5220 6156 6186 1178 Size: 30 936 30 4098 Fragments arranged by size: 4098 2818 936 867 165 42 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2090 2093 2096 2099 2102 2105 2120 2517 Size: 904 3 3 3 3 3 15 397 Cuts at: 2517 2642 2789 3221 3700 1186 Size: 125 147 432 479 6592 Fragments arranged by size: 6592 904 479 432 397 147 125 15 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 3823 4415 5942 6143 6221 6283 8309 8805 3823 Size: 592 1527 201 78 62 2026 496 4124 Fragments arranged by size: 4124 2026 1527 592 496 201 78 62 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2624 3641 3701 4709 5102 5214 5228 Size: 573 888 1017 60 1008 393 112 14 Cuts at: 5228 5867 1163 Size: 639 4402 Fragments arranged by size: 4402 1017 1008 888 639 573 393 112 60 14 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6556 7317 8345 1540 Size: 275 186 4555 761 1028 2301 Fragments arranged by size: 4555 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2219 2314 2621 3144 4324 5588 7167 7252 8323 Size: 95 307 523 1180 1264 1579 85 1071 Cuts at: 8323 2219 Size: 3002 Fragments arranged by size: 3002 1579 1264 1180 1071 523 307 95 85 BsmI GAATG_Cn' Cuts at: 3845 6617 6710 3845 Size: 2772 93 6241 Fragments arranged by size: 6241 2772 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5512 5905 5512 Size: 393 8713 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2219 2314 2621 3144 3970 4324 5588 Size: 399 429 95 307 523 826 354 1264 Cuts at: 5588 6425 7167 7252 8323 1391 Size: 837 742 85 1071 2174 Fragments arranged by size: 2174 1264 1071 837 826 742 523 429 399 354 307 95 85 BspEI T'CCGG_A Cuts at: 8769 8769 Size: 9106 BspHI T'CATG_A Cuts at: 6328 6995 8725 6328 Size: 667 1730 6709 Fragments arranged by size: 6709 1730 667 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2142 5797 6461 844 Size: 1298 3655 664 3489 Fragments arranged by size: 3655 3489 1298 664 BsrBI CCG'CTC Cuts at: 1394 1823 3453 4390 6993 1394 Size: 429 1630 937 2603 3507 Fragments arranged by size: 3507 2603 1630 937 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3605 4853 5689 7601 7775 65 Size: 1183 2357 1248 836 1912 174 1396 Fragments arranged by size: 2357 1912 1396 1248 1183 836 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3010 5811 7750 7937 1700 Size: 110 1200 2801 1939 187 2869 Fragments arranged by size: 2869 2801 1939 1200 187 110 BsrGI T'GTAC_A Cuts at: 96 3222 5266 96 Size: 3126 2044 3936 Fragments arranged by size: 3936 3126 2044 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9106 BssSI C'ACGA_G Cuts at: 3159 7166 8178 3159 Size: 4007 1012 4087 Fragments arranged by size: 4087 4007 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3161 5746 794 Size: 2367 2585 4154 Fragments arranged by size: 4154 2585 2367 BstBI TT'CG_AA Cuts at: 4336 7910 4336 Size: 3574 5532 BstEII G'GTnAC_C Cuts at: 1184 5218 1184 Size: 4034 5072 BstXI CCAn_nnnn'nTGG Cuts at: 4495 5729 6001 4495 Size: 1234 272 7600 Fragments arranged by size: 7600 1234 272 BstYI r'GATC_y Cuts at: 1972 2838 3759 3864 4221 4623 5349 6436 6803 Size: 866 921 105 357 402 726 1087 367 Cuts at: 6803 7188 7205 8657 8743 8755 9101 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1087 921 866 726 402 385 367 357 346 105 86 17 12 BstZ17I GTA'TAC Cuts at: 3777 4555 3777 Size: 778 8328 Bsu36I CC'TnA_GG Cuts at: 2023 2484 3618 5410 5487 2023 Size: 461 1134 1792 77 5642 Fragments arranged by size: 5642 1792 1134 461 77 BtgI C'CryG_G Cuts at: 513 1065 2210 5308 6075 513 Size: 552 1145 3098 767 3544 Fragments arranged by size: 3544 3098 1145 767 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8367 Fragments arranged by size: 8367 683 56 BtsI GCAGTG_nn' Cuts at: 2106 2575 2581 2662 2899 5647 6090 6622 7416 Size: 469 6 81 237 2748 443 532 794 Cuts at: 7416 7436 2106 Size: 20 3776 Fragments arranged by size: 3776 2748 794 532 469 443 237 81 20 6 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9106 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9071 DraI TTT'AAA Cuts at: 3118 6410 3118 Size: 3292 5814 DraIII CAC_nnn'GTG Cuts at: 2647 3700 5746 2647 Size: 1053 2046 6007 Fragments arranged by size: 6007 2046 1053 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5685 8113 809 Size: 507 4369 2428 1802 Fragments arranged by size: 4369 2428 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3915 4564 5064 Size: 54 1425 50 275 18 2085 649 500 Cuts at: 5064 5496 5719 5778 6553 7442 9071 8 Size: 432 223 59 775 889 1629 43 Fragments arranged by size: 2085 1629 1425 889 775 649 500 432 275 223 59 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6553 1537 Size: 275 4741 4090 Fragments arranged by size: 4741 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2067 2300 2394 2682 2975 3027 6076 7036 Size: 714 233 94 288 293 52 3049 960 Cuts at: 7036 1353 Size: 3423 Fragments arranged by size: 3423 3049 960 714 293 288 233 94 52 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2963 3617 7693 8067 8213 387 Size: 1064 138 1374 654 4076 374 146 1280 Fragments arranged by size: 4076 1374 1280 1064 654 374 146 138 EcoICRI GAG'CTC Cuts at: 2312 2619 5586 2312 Size: 307 2967 5832 Fragments arranged by size: 5832 2967 307 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2081 2232 2444 2492 2643 2754 2894 Size: 393 449 151 212 48 151 111 140 Cuts at: 2894 3008 3087 3114 3260 3461 3689 3725 3884 Size: 114 79 27 146 201 228 36 159 Cuts at: 3884 4167 4829 4999 5131 5409 5525 7169 7766 Size: 283 662 170 132 278 116 1644 597 Cuts at: 7766 8553 8821 1239 Size: 787 268 1524 Fragments arranged by size: 1644 1524 787 662 597 449 393 283 278 268 228 212 201 170 159 151 151 146 140 132 116 114 111 79 48 36 27 EcoNI CCTnn'n_nnAGG Cuts at: 2483 3902 4193 4381 5679 2483 Size: 1419 291 188 1298 5910 Fragments arranged by size: 5910 1419 1298 291 188 EcoO109I rG'GnC_Cy Cuts at: 2293 2746 3928 4123 5423 6056 2293 Size: 453 1182 195 1300 633 5343 Fragments arranged by size: 5343 1300 1182 633 453 195 EcoRI G'AATT_C Cuts at: 4268 6415 6793 4268 Size: 2147 378 6581 Fragments arranged by size: 6581 2147 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3928 3928 Size: 9106 FspI TGC'GCA Cuts at: 4968 7612 4968 Size: 2644 6462 HaeII r_GCGC'y Cuts at: 1678 2266 2422 8253 1678 Size: 588 156 5831 2531 Fragments arranged by size: 5831 2531 588 156 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2132 2164 3142 3170 3174 Size: 32 135 32 560 32 978 28 4 Cuts at: 3174 3202 3390 3422 3856 3888 4835 4867 7747 Size: 28 188 32 434 32 947 32 2880 Cuts at: 7747 7779 7821 7853 1373 Size: 32 42 32 2626 Fragments arranged by size: 2880 2626 978 947 560 434 188 135 42 32 32 32 32 32 32 32 32 28 28 4 HincII GTy'rAC Cuts at: 669 1657 1938 2755 6450 6696 669 Size: 988 281 817 3695 246 3079 Fragments arranged by size: 3695 3079 988 817 281 246 HindIII A'AGCT_T Cuts at: 748 4208 748 Size: 3460 5646 HpaI GTT'AAC Cuts at: 6696 6696 Size: 9106 KasI G'GCGC_C Cuts at: 2262 2262 Size: 9106 KpnI G_GTAC'C Cuts at: 3484 3643 4308 6431 3484 Size: 159 665 2123 6159 Fragments arranged by size: 6159 2123 665 159 MfeI C'AATT_G Cuts at: 2366 6705 2366 Size: 4339 4767 MluI A'CGCG_T Cuts at: 8982 8982 Size: 9106 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2385 5378 8220 8404 1305 Size: 1080 2993 2842 184 2007 Fragments arranged by size: 2993 2842 2007 1080 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3917 4566 5066 5498 5721 Size: 54 1425 343 2085 649 500 432 223 Cuts at: 5721 5780 10 Size: 59 3336 Fragments arranged by size: 3336 2085 1425 649 500 432 343 223 59 54 MslI CAynn'nnrTG Cuts at: 518 1166 2709 3879 4111 4327 5099 7064 7423 Size: 648 1543 1170 232 216 772 1965 359 Cuts at: 7423 7582 518 Size: 159 2042 Fragments arranged by size: 2042 1965 1543 1170 772 648 359 232 216 159 MspA1I CmG'CkG Cuts at: 1606 2212 2470 3816 3972 5989 6287 6522 7199 Size: 606 258 1346 156 2017 298 235 677 Cuts at: 7199 8347 8592 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 2017 1346 1148 677 606 298 258 245 235 156 NaeI GCC'GGC Cuts at: 5813 5813 Size: 9106 NarI GG'CG_CC Cuts at: 2263 2263 Size: 9106 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 8554 NdeI CA'TA_TG Cuts at: 387 4108 387 Size: 3721 5385 NgoMIV G'CCGG_C Cuts at: 5811 5811 Size: 9106 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9106 NotI GC'GGCC_GC Cuts at: 6553 6553 Size: 9106 NspI r_CATG'y Cuts at: 1132 1229 2164 2180 3052 3574 4657 5117 6244 Size: 97 935 16 872 522 1083 460 1127 Cuts at: 6244 6265 6275 6464 8009 1132 Size: 21 10 189 1545 2229 Fragments arranged by size: 2229 1545 1127 1083 935 872 522 460 189 97 21 16 10 PasI CC'CwG_GG Cuts at: 1348 5419 1348 Size: 4071 5035 PciI A'CATG_T Cuts at: 3048 3570 6261 8005 3048 Size: 522 2691 1744 4149 Fragments arranged by size: 4149 2691 1744 522 PflMI CCAn_nnn'nTGG Cuts at: 1108 2923 5632 5876 6290 1108 Size: 1815 2709 244 414 3924 Fragments arranged by size: 3924 2709 1815 414 244 PfoI T'CCnGG_A Cuts at: 2355 3798 4080 4514 5242 5819 2355 Size: 1443 282 434 728 577 5642 Fragments arranged by size: 5642 1443 728 577 434 282 PmeI GTTT'AAAC Cuts at: 6410 6410 Size: 9106 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2270 2302 5659 5691 6332 6364 7175 7207 8688 Size: 32 3357 32 641 32 811 32 1481 Cuts at: 8688 8720 2270 Size: 32 2656 Fragments arranged by size: 3357 2656 1481 811 641 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2293 2746 3928 4123 5423 2293 Size: 453 1182 195 1300 5976 Fragments arranged by size: 5976 1300 1182 453 195 PshAI GACnn'nnGTC Cuts at: 1613 4148 1613 Size: 2535 6571 PsiI TTA'TAA Cuts at: 6676 6676 Size: 9106 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3767 3799 3767 Size: 32 9074 PstI C_TGCA'G Cuts at: 830 3958 6458 830 Size: 3128 2500 3478 Fragments arranged by size: 3478 3128 2500 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9106 PvuII CAG'CTG Cuts at: 5989 6287 5989 Size: 298 8808 SacI G_AGCT'C Cuts at: 2314 2621 5588 2314 Size: 307 2967 5832 Fragments arranged by size: 5832 2967 307 SacII CC_GC'GG Cuts at: 2213 2213 Size: 9106 SalI G'TCGA_C Cuts at: 1936 6448 1936 Size: 4512 4594 SbfI CC_TGCA'GG Cuts at: 6458 6458 Size: 9106 ScaI AGT'ACT Cuts at: 3509 7354 9066 3509 Size: 3845 1712 3549 Fragments arranged by size: 3845 3549 1712 SexAI A'CCwGG_T Cuts at: 4300 4300 Size: 9106 SfcI C'TryA_G Cuts at: 826 1045 3954 4055 6454 7589 8270 8461 826 Size: 219 2909 101 2399 1135 681 191 1471 Fragments arranged by size: 2909 2399 1471 1135 681 219 191 101 SfoI GGC'GCC Cuts at: 2264 2264 Size: 9106 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9106 SgrAI Cr'CCGG_yG Cuts at: 1700 5811 1700 Size: 4111 4995 SmaI CCC'GGG Cuts at: 1872 5570 5653 6433 1872 Size: 3698 83 780 4545 Fragments arranged by size: 4545 3698 780 83 SmlI C'TyrA_G Cuts at: 820 1017 1943 3839 5755 7210 8111 8373 820 Size: 197 926 1896 1916 1455 901 262 1553 Fragments arranged by size: 1916 1896 1553 1455 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 6897 493 Size: 6404 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9106 SphI G_CATG'C Cuts at: 1132 2180 6464 1132 Size: 1048 4284 3774 Fragments arranged by size: 4284 3774 1048 SspI AAT'ATT Cuts at: 5 52 7030 5 Size: 47 6978 2081 Fragments arranged by size: 6978 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 9106 StyI C'CwwG_G Cuts at: 513 1065 2269 2737 3544 3723 4014 4119 4251 Size: 552 1204 468 807 179 291 105 132 Cuts at: 4251 4273 4830 4887 6545 513 Size: 22 557 57 1658 3074 Fragments arranged by size: 3074 1658 1204 807 557 552 468 291 179 132 105 57 22 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2717 3172 3280 3306 4457 5485 5757 Size: 1255 1137 455 108 26 1151 1028 272 Cuts at: 5757 7133 7150 7303 7488 7933 325 Size: 1376 17 153 185 445 1498 Fragments arranged by size: 1498 1376 1255 1151 1137 1028 455 445 272 185 153 108 26 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3222 3507 5266 Size: 274 80 33 51 1443 1245 285 1759 Cuts at: 5266 7352 9064 96 Size: 2086 1712 138 Fragments arranged by size: 2086 1759 1712 1443 1245 285 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2147 2179 5190 5222 6156 6188 9066 9098 2147 Size: 32 3011 32 934 32 2878 32 2155 Fragments arranged by size: 3011 2878 2155 934 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 9106 XbaI T'CTAG_A Cuts at: 3307 4612 6442 3307 Size: 1305 1830 5971 Fragments arranged by size: 5971 1830 1305 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4495 5306 4495 Size: 811 8295 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9106 XmaI C'CCGG_G Cuts at: 1870 5568 5651 6431 1870 Size: 3698 83 780 4545 Fragments arranged by size: 4545 3698 780 83 XmnI GAAnn'nnTTC Cuts at: 3897 7235 3897 Size: 3338 5768 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2965 3625 6914 276 Size: 53 83 186 936 1431 660 3289 2468 Fragments arranged by size: 3289 2468 1431 936 660 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BbvCI BfrBI BplI BsiWI EcoRV FseI FspAI NruI NsiI PacI PmlI PspOMI PspXI RsrII SanDI SapI SfiI SrfI SwaI