Restriction map for
pFN21AE9641
(Circular) (Six-base) MAPSORT of: pFN21AE9641.seq Check: 4825 from: 1 to: 5893
pFN21AE9641 5893 bp
With 182 enzymes: SgfI *
March 28, 2012 12:19 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2867 3703 278
Size: 53 83 186 936 1331 836 2468
Fragments arranged by size:
2468 1331 936 836 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2343 3021 3236 1937
Size: 406 678 215 4594
Fragments arranged by size:
4594 678 406 215
Acc65I G'GTAC_C
Cuts at: 3214 3214
Size: 5893
AclI AA'CG_TT
Cuts at: 1563 4020 4393 1563
Size: 2457 373 3063
Fragments arranged by size:
3063 2457 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2199 2711 3956 5340 5608 1632
Size: 567 512 1245 1384 268 1917
Fragments arranged by size:
1917 1384 1245 567 512 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5696
AflIII A'CryG_T
Cuts at: 1203 4792 5769 1203
Size: 3589 977 1327
Fragments arranged by size:
3589 1327 977
AgeI A'CCGG_T
Cuts at: 4724 4724
Size: 5893
AhdI GACnn_n'nnGTC
Cuts at: 4622 4622
Size: 5893
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5893
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2226 1184
Size: 1042 4851
AlwNI CAG_nnn'CTG
Cuts at: 2689 2774 3636 5208 2689
Size: 85 862 1572 3374
Fragments arranged by size:
3374 1572 862 85
ApaLI G'TGCA_C
Cuts at: 3950 5106 3950
Size: 1156 4737
ApoI r'AATT_y
Cuts at: 1493 3202 3428 3580 5689 1493
Size: 1709 226 152 2109 1697
Fragments arranged by size:
2109 1709 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4447 160
Size: 872 3415 1606
Fragments arranged by size:
3415 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5893
AvaI C'yCGr_G
Cuts at: 1870 1943 3218 1870
Size: 73 1275 4545
Fragments arranged by size:
4545 1275 73
BamHI G'GATC_C
Cuts at: 3223 3590 3223
Size: 367 5526
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3214 4669 618
Size: 325 217 627 1427 1455 1842
Fragments arranged by size:
1842 1455 1427 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3212 1391
Size: 1821 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4922
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4084 4118 1903
Size: 34 2147 34 3678
Fragments arranged by size:
3678 2147 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3785 4995 3785
Size: 1210 4683
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5893
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4504 136
Size: 107 122 71 631 605 2832 1525
Fragments arranged by size:
2832 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2140 5888 2140
Size: 3748 2145
BlpI GC'TnA_GC
Cuts at: 3310 3310
Size: 5893
Bme1580I G_kGCm'C
Cuts at: 1790 3954 5110 1790
Size: 2164 1156 2573
Fragments arranged by size:
2573 2164 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5893
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3624 4582 442
Size: 416 53 83 399 24 2207 958 1753
Fragments arranged by size:
2207 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5893
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2907 3061 4553 1239
Size: 1668 154 1492 2579
Fragments arranged by size:
2579 1668 1492 154
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2279 3075 3148 4018 4883 5181 2279
Size: 796 73 870 865 298 2991
Fragments arranged by size:
2991 870 865 796 298 73
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4556 882
Size: 617 3057 2219
Fragments arranged by size:
3057 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2989 3684 493
Size: 711 1785 695 2702
Fragments arranged by size:
2702 1785 711 695
BsaBI GATnn'nnATC
Cuts at: 5887 5887
Size: 5893
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2864 3700 4082 4717
Size: 53 83 186 936 1331 836 382 635
Cuts at: 4717 275
Size: 1451
Fragments arranged by size:
1451 1331 936 836 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4326 4724 4998 5145 5556 4326
Size: 398 274 147 411 4663
Fragments arranged by size:
4663 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2117 2147 3059 3089 1178
Size: 30 165 30 714 30 912 30 3982
Fragments arranged by size:
3982 912 714 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2603 2839 2972 2988 3110 1186
Size: 1417 236 133 16 122 3969
Fragments arranged by size:
3969 1417 236 133 122 16
BseYI C'CCAG_C
Cuts at: 2347 5096 5592 2347
Size: 2749 496 2648
Fragments arranged by size:
2749 2648 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2196 1163
Size: 573 460 4860
Fragments arranged by size:
4860 573 460
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3343 4104 5132 1540
Size: 275 186 1342 761 1028 2301
Fragments arranged by size:
2301 1342 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3954 4039 5110 3954
Size: 85 1071 4737
Fragments arranged by size:
4737 1071 85
BsmI GAATG_Cn'
Cuts at: 2496 3404 3497 2496
Size: 908 93 4892
Fragments arranged by size:
4892 908 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3212 3954 4039 5110 1391
Size: 399 1422 742 85 1071 2174
Fragments arranged by size:
2174 1422 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5556 5556
Size: 5893
BspHI T'CATG_A
Cuts at: 3082 3782 5512 3082
Size: 700 1730 3463
Fragments arranged by size:
3463 1730 700
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3248 844
Size: 2404 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3780 1394
Size: 429 1957 3507
Fragments arranged by size:
3507 1957 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4388 4562 65
Size: 1183 3140 174 1396
Fragments arranged by size:
3140 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4537 4724 1700
Size: 110 2727 187 2869
Fragments arranged by size:
2869 2727 187 110
BsrGI T'GTAC_A
Cuts at: 96 2113 3175 96
Size: 2017 1062 2814
Fragments arranged by size:
2814 2017 1062
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5893
BssSI C'ACGA_G
Cuts at: 3953 4965 3953
Size: 1012 4881
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5893
BstBI TT'CG_AA
Cuts at: 4697 4697
Size: 5893
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5893
BstYI r'GATC_y
Cuts at: 1972 2140 3223 3590 3975 3992 5444 5530 5542
Size: 168 1083 367 385 17 1452 86 12
Cuts at: 5542 5888 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1083 385 367 346 168 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 3030 513
Size: 552 1965 3376
Fragments arranged by size:
3376 1965 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5154
Fragments arranged by size:
5154 683 56
BtsI GCAGTG_nn'
Cuts at: 3043 3111 3409 4203 4223 3043
Size: 68 298 794 20 4713
Fragments arranged by size:
4713 794 298 68 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5893
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5858
DraI TTT'AAA
Cuts at: 3197 3197
Size: 5893
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2869 4900 809
Size: 507 1553 2031 1802
Fragments arranged by size:
2031 1802 1553 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3340 4229 5858
Size: 54 1425 50 275 18 1510 889 1629
Cuts at: 5858 8
Size: 43
Fragments arranged by size:
1629 1510 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3340 1537
Size: 275 1528 4090
Fragments arranged by size:
4090 1528 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2028 3823 1353
Size: 675 1795 3423
Fragments arranged by size:
3423 1795 675
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4480 4854 5000 387
Size: 1064 138 2891 374 146 1280
Fragments arranged by size:
2891 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2199 2711 2907 3061 3956 4553 5340
Size: 393 567 512 196 154 895 597 787
Cuts at: 5340 5608 1239
Size: 268 1524
Fragments arranged by size:
1524 895 787 597 567 512 393 268
196 154
EcoRI G'AATT_C
Cuts at: 3202 3580 3202
Size: 378 5515
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2039 2039
Size: 5893
FspI TGC'GCA
Cuts at: 4399 4399
Size: 5893
HaeII r_GCGC'y
Cuts at: 1678 5040 1678
Size: 3362 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2132 2164 3059 3091 4534
Size: 32 135 32 560 32 895 32 1443
Cuts at: 4534 4566 4608 4640 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1443 895 560 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3237 3483 669
Size: 988 281 1299 246 3079
Fragments arranged by size:
3079 1299 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2118 748
Size: 1370 4523
HpaI GTT'AAC
Cuts at: 3483 3483
Size: 5893
KpnI G_GTAC'C
Cuts at: 3218 3218
Size: 5893
MfeI C'AATT_G
Cuts at: 3492 3492
Size: 5893
MluI A'CGCG_T
Cuts at: 5769 5769
Size: 5893
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2970 5007 5191 1305
Size: 1665 2037 184 2007
Fragments arranged by size:
2037 2007 1665 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4071
Fragments arranged by size:
4071 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3851 4210 4369 518
Size: 648 2685 359 159 2042
Fragments arranged by size:
2685 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2527 2689 3309 3986 5134 5379 1606
Size: 921 162 620 677 1148 245 2120
Fragments arranged by size:
2120 1148 921 677 620 245 162
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5341
NdeI CA'TA_TG
Cuts at: 387 2110 387
Size: 1723 4170
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5893
NotI GC'GGCC_GC
Cuts at: 3340 3340
Size: 5893
NspI r_CATG'y
Cuts at: 1132 1229 3251 4796 1132
Size: 97 2022 1545 2229
Fragments arranged by size:
2229 2022 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2533 1348
Size: 1185 4708
PciI A'CATG_T
Cuts at: 4792 4792
Size: 5893
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2710 2840 2930 1108
Size: 1602 130 90 4071
Fragments arranged by size:
4071 1602 130 90
PfoI T'CCnGG_A
Cuts at: 2499 2499
Size: 5893
PmeI GTTT'AAAC
Cuts at: 3197 3197
Size: 5893
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3962 3994 5475 5507 3962
Size: 32 1481 32 4348
Fragments arranged by size:
4348 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2814 3078 1613
Size: 1201 264 4428
Fragments arranged by size:
4428 1201 264
PsiI TTA'TAA
Cuts at: 3463 3463
Size: 5893
PstI C_TGCA'G
Cuts at: 830 2682 2816 3245 830
Size: 1852 134 429 3478
Fragments arranged by size:
3478 1852 429 134
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5893
PvuII CAG'CTG
Cuts at: 2527 2689 2527
Size: 162 5731
SalI G'TCGA_C
Cuts at: 1936 3235 1936
Size: 1299 4594
SbfI CC_TGCA'GG
Cuts at: 3245 3245
Size: 5893
ScaI AGT'ACT
Cuts at: 4141 5853 4141
Size: 1712 4181
SfcI C'TryA_G
Cuts at: 826 1045 2678 2766 2781 2812 3241 4376 5057
Size: 219 1633 88 15 31 429 1135 681
Cuts at: 5057 5248 826
Size: 191 1471
Fragments arranged by size:
1633 1471 1135 681 429 219 191 88
31 15
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5893
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5893
SmaI CCC'GGG
Cuts at: 1872 3220 1872
Size: 1348 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2294 3090 3163 3997 4898 5160
Size: 197 926 351 796 73 834 901 262
Cuts at: 5160 820
Size: 1553
Fragments arranged by size:
1553 926 901 834 796 351 262 197
73
SnaBI TAC'GTA
Cuts at: 493 2989 3684 493
Size: 2496 695 2702
Fragments arranged by size:
2702 2496 695
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5893
SphI G_CATG'C
Cuts at: 1132 3251 1132
Size: 2119 3774
SspI AAT'ATT
Cuts at: 5 52 3817 5
Size: 47 3765 2081
Fragments arranged by size:
3765 2081 47
StuI AGG'CCT
Cuts at: 1517 2741 1517
Size: 1224 4669
StyI C'CwwG_G
Cuts at: 513 1065 2250 3332 513
Size: 552 1185 1082 3074
Fragments arranged by size:
3074 1185 1082 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3920 3937 4090 4275 4720 325
Size: 1255 2340 17 153 185 445 1498
Fragments arranged by size:
2340 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2113 2133 2273
Size: 274 80 33 51 1443 136 20 140
Cuts at: 2273 3175 4139 5851 96
Size: 902 964 1712 138
Fragments arranged by size:
1712 1443 964 902 274 140 138 136
80 51 33 20
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5853 5885 5853
Size: 32 5861
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5893
XbaI T'CTAG_A
Cuts at: 3229 3229
Size: 5893
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2864 3149 2864
Size: 285 5608
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5893
XmaI C'CCGG_G
Cuts at: 1870 3218 1870
Size: 1348 4545
XmnI GAAnn'nnTTC
Cuts at: 2022 2621 4022 2022
Size: 599 1401 3893
Fragments arranged by size:
3893 1401 599
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2865 3701 276
Size: 53 83 186 936 1331 836 2468
Fragments arranged by size:
2468 1331 936 836 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI