Restriction map for
pFN21AE9635
(Circular) (Six-base) MAPSORT of: pFN21AE9635.seq Check: 3566 from: 1 to: 9337
pFN21AE9635 9337 bp
With 182 enzymes: SgfI *
March 28, 2012 14:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2779 4825 7147 278
Size: 53 83 186 936 1243 2046 2322 2468
Fragments arranged by size:
2468 2322 2046 1243 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 6680 1937
Size: 4743 4594
Acc65I G'GTAC_C
Cuts at: 5620 6658 5620
Size: 1038 8299
AclI AA'CG_TT
Cuts at: 1563 7464 7837 1563
Size: 5901 373 3063
Fragments arranged by size:
5901 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 5051 5406 5705 6254 7400 8784 9052 1632
Size: 3419 355 299 549 1146 1384 268 1917
Fragments arranged by size:
3419 1917 1384 1146 549 355 299 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9140
AflIII A'CryG_T
Cuts at: 1203 2368 5098 8236 9213 1203
Size: 1165 2730 3138 977 1327
Fragments arranged by size:
3138 2730 1327 1165 977
AgeI A'CCGG_T
Cuts at: 8168 8168
Size: 9337
AhdI GACnn_n'nnGTC
Cuts at: 8066 8066
Size: 9337
AleI CACnn'nnGTG
Cuts at: 1166 2095 3543 1166
Size: 929 1448 6960
Fragments arranged by size:
6960 1448 929
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2458 1184
Size: 1274 8063
AlwNI CAG_nnn'CTG
Cuts at: 2272 4894 5061 5641 5809 7080 8652 2272
Size: 2622 167 580 168 1271 1572 2957
Fragments arranged by size:
2957 2622 1572 1271 580 168 167
ApaI G_GGCC'C
Cuts at: 2063 2069 2063
Size: 6 9331
ApaLI G'TGCA_C
Cuts at: 7394 8550 7394
Size: 1156 8181
ApoI r'AATT_y
Cuts at: 1493 2308 2361 2535 3230 3302 3382 4373 4700
Size: 815 53 174 695 72 80 991 327
Cuts at: 4700 5164 5245 5697 5831 6090 6646 6872 7024
Size: 464 81 452 134 259 556 226 152
Cuts at: 7024 9133 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 991 815 695 556 464 452
327 259 226 174 152 134 81 80
72 53
AseI AT'TA_AT
Cuts at: 160 1032 7891 160
Size: 872 6859 1606
Fragments arranged by size:
6859 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9337
AvaI C'yCGr_G
Cuts at: 1870 1943 2054 2062 2093 2738 6662 1870
Size: 73 111 8 31 645 3924 4545
Fragments arranged by size:
4545 3924 645 111 73 31 8
AvrII C'CTAG_G
Cuts at: 4834 4834
Size: 9337
BamHI G'GATC_C
Cuts at: 6667 7034 6667
Size: 367 8970
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 5620 6658 8113 618
Size: 325 217 627 3833 1038 1455 1842
Fragments arranged by size:
3833 1842 1455 1038 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2063 2069 2510 4530 5337 5803 5855 6656
Size: 672 6 441 2020 807 466 52 801
Cuts at: 6656 1391
Size: 4072
Fragments arranged by size:
4072 2020 807 801 672 466 441 52
6
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2098 2973 3513 6444 6594 928
Size: 971 199 875 540 2931 150 3671
Fragments arranged by size:
3671 2931 971 875 540 199 150
BbvCI CC'TCA_GC
Cuts at: 3795 5196 5884 3795
Size: 1401 688 7248
Fragments arranged by size:
7248 1401 688
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 7528 7562 1903
Size: 34 5591 34 3678
Fragments arranged by size:
5591 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2273 4748 5738 6023 6088 7229 8439 2273
Size: 2475 990 285 65 1141 1210 3171
Fragments arranged by size:
3171 2475 1210 1141 990 285 65
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9337
BfrBI ATG'CAT
Cuts at: 4244 4244
Size: 9337
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 2073 2212
Size: 107 122 71 631 605 337 64 139
Cuts at: 2212 7948 136
Size: 5736 1525
Fragments arranged by size:
5736 1525 631 605 337 139 122 107
71 64
BglII A'GATC_T
Cuts at: 2382 9332 2382
Size: 6950 2387
BlpI GC'TnA_GC
Cuts at: 3585 6135 6754 3585
Size: 2550 619 6168
Fragments arranged by size:
6168 2550 619
Bme1580I G_kGCm'C
Cuts at: 1790 2063 2069 2102 2681 7398 8554 1790
Size: 273 6 33 579 4717 1156 2573
Fragments arranged by size:
4717 2573 1156 579 273 33 6
BmgBI CAC'GTC
Cuts at: 1318 5099 1318
Size: 3781 5556
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2344 3550 4924
Size: 416 53 83 399 24 927 1206 1374
Cuts at: 4924 5619 5968 6517 7068 8026 442
Size: 695 349 549 551 958 1753
Fragments arranged by size:
1753 1374 1206 958 927 695 551 549
416 399 349 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9337
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6275 6277 6275
Size: 2 9335
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3076 3535 3603 5112 5408 6056 6247 7997
Size: 1837 459 68 1509 296 648 191 1750
Cuts at: 7997 1239
Size: 2579
Fragments arranged by size:
2579 1837 1750 1509 648 459 296 191
68
Bpu10I CC'TnA_GC
Cuts at: 3078 3795 5196 5854 5884 3078
Size: 717 1401 658 30 6531
Fragments arranged by size:
6531 1401 717 658 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4925 6242 7462 8327 8625 4925
Size: 1317 1220 865 298 5637
Fragments arranged by size:
5637 1317 1220 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6157 8000 882
Size: 617 4658 1843 2219
Fragments arranged by size:
4658 2219 1843 617
BsaAI yAC'GTr
Cuts at: 493 1204 2261 7128 493
Size: 711 1057 4867 2702
Fragments arranged by size:
4867 2702 1057 711
BsaBI GATnn'nnATC
Cuts at: 4483 4736 9331 4483
Size: 253 4595 4489
Fragments arranged by size:
4595 4489 253
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2776 4822 6241 7144
Size: 53 83 186 936 1243 2046 1419 903
Cuts at: 7144 7526 8161 275
Size: 382 635 1451
Fragments arranged by size:
2046 1451 1419 1243 936 903 635 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 2564 7770 8168 8442 8589 9000 2564
Size: 5206 398 274 147 411 2901
Fragments arranged by size:
5206 2901 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 4781 4811 5085 5115 6486
Size: 30 165 30 3378 30 274 30 1371
Cuts at: 6486 6516 1178
Size: 30 3999
Fragments arranged by size:
3999 3378 1371 274 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2756 3272 3374 3638 4928 1186
Size: 1570 516 102 264 1290 5595
Fragments arranged by size:
5595 1570 1290 516 264 102
BseYI C'CCAG_C
Cuts at: 2071 2114 2118 2683 3636 3728 4793 5557 5802
Size: 43 4 565 953 92 1065 764 245
Cuts at: 5802 6063 6167 6466 8540 9036 2071
Size: 261 104 299 2074 496 2372
Fragments arranged by size:
2372 2074 1065 953 764 565 496 299
261 245 104 92 43 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2009 2681 3796 4793 5066 1163
Size: 573 273 672 1115 997 273 5434
Fragments arranged by size:
5434 1115 997 672 573 273 273
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2201 6787 7548 8576 1540
Size: 275 186 200 4586 761 1028 2301
Fragments arranged by size:
4586 2301 1028 761 275 200 186
BsiHKAI G_wGCw'C
Cuts at: 4530 4914 7398 7483 8554 4530
Size: 384 2484 85 1071 5313
Fragments arranged by size:
5313 2484 1071 384 85
BsmI GAATG_Cn'
Cuts at: 4246 6848 6941 4246
Size: 2602 93 6642
Fragments arranged by size:
6642 2602 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2767 4813 4829 6232 2767
Size: 2046 16 1403 5872
Fragments arranged by size:
5872 2046 1403 16
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2063 2069 2102 2510 2681 4530 4914
Size: 399 273 6 33 408 171 1849 384
Cuts at: 4914 5337 5803 5855 6656 7398 7483 8554 1391
Size: 423 466 52 801 742 85 1071 2174
Fragments arranged by size:
2174 1849 1071 801 742 466 423 408
399 384 273 171 85 52 33 6
BspEI T'CCGG_A
Cuts at: 2564 9000 2564
Size: 6436 2901
BspHI T'CATG_A
Cuts at: 3669 3694 7226 8956 3669
Size: 25 3532 1730 4050
Fragments arranged by size:
4050 3532 1730 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 6692 844
Size: 5848 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 6271 7224 1394
Size: 429 4448 953 3507
Fragments arranged by size:
4448 3507 953 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2965 3288 7832 8006 65
Size: 1183 1717 323 4544 174 1396
Fragments arranged by size:
4544 1717 1396 1183 323 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2970 7981 8168 1700
Size: 110 1160 5011 187 2869
Fragments arranged by size:
5011 2869 1160 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9337
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9337
BssSI C'ACGA_G
Cuts at: 2295 5136 7397 8409 2295
Size: 2841 2261 1012 3223
Fragments arranged by size:
3223 2841 2261 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2659 4453 4681 4894 794
Size: 1865 1794 228 213 5237
Fragments arranged by size:
5237 1865 1794 228 213
BstBI TT'CG_AA
Cuts at: 2109 8141 2109
Size: 6032 3305
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 9337
BstXI CCAn_nnnn'nTGG
Cuts at: 2923 3558 5614 6521 2923
Size: 635 2056 907 5739
Fragments arranged by size:
5739 2056 907 635
BstYI r'GATC_y
Cuts at: 1972 2157 2382 3643 4594 5001 6667 7034 7419
Size: 185 225 1261 951 407 1666 367 385
Cuts at: 7419 7436 8888 8974 8986 9332 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1666 1452 1261 951 407 385 367
346 225 185 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2576 3351 5453 5745 2576
Size: 775 2102 292 6168
Fragments arranged by size:
6168 2102 775 292
BtgI C'CryG_G
Cuts at: 513 1065 2004 6022 513
Size: 552 939 4018 3828
Fragments arranged by size:
4018 3828 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 8598
Fragments arranged by size:
8598 683 56
BtsI GCAGTG_nn'
Cuts at: 2021 2508 2959 3316 4869 6178 6853 7647 7667
Size: 487 451 357 1553 1309 675 794 20
Cuts at: 7667 2021
Size: 3691
Fragments arranged by size:
3691 1553 1309 794 675 487 451 357
20
ClaI AT'CG_AT
Cuts at: 1554 3871 4483 1554
Size: 2317 612 6408
Fragments arranged by size:
6408 2317 612
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3199 3234 5591 5626 529
Size: 35 2635 35 2357 35 4240
Fragments arranged by size:
4240 2635 2357 35 35 35
DraI TTT'AAA
Cuts at: 6641 6641
Size: 9337
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2933 8344 809
Size: 507 1617 5411 1802
Fragments arranged by size:
5411 1802 1617 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 2198 3969
Size: 54 1425 50 275 18 183 185 1771
Cuts at: 3969 6784 7673 9302 8
Size: 2815 889 1629 43
Fragments arranged by size:
2815 1771 1629 1425 889 275 185 183
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2198 6784 1537
Size: 275 386 4586 4090
Fragments arranged by size:
4586 4090 386 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2118 2819 3351 4563 4761 4992 6230 7267
Size: 765 701 532 1212 198 231 1238 1037
Cuts at: 7267 1353
Size: 3423
Fragments arranged by size:
3423 1238 1212 1037 765 701 532 231
198
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 7924 8298 8444 387
Size: 1064 138 6335 374 146 1280
Fragments arranged by size:
6335 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4528 4528
Size: 9337
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3076 3535 3603 5051 5112 5406 5408
Size: 393 1444 459 68 1448 61 294 2
Cuts at: 5408 5705 6056 6247 6254 7400 7997 8784 9052
Size: 297 351 191 7 1146 597 787 268
Cuts at: 9052 1239
Size: 1524
Fragments arranged by size:
1524 1448 1444 1146 787 597 459 393
351 297 294 268 191 68 61 7
2
EcoNI CCTnn'n_nnAGG
Cuts at: 5744 5744
Size: 9337
EcoO109I rG'GnC_Cy
Cuts at: 2059 5080 5554 5638 6256 2059
Size: 3021 474 84 618 5140
Fragments arranged by size:
5140 3021 618 474 84
EcoRI G'AATT_C
Cuts at: 2535 5164 5245 6646 7024 2535
Size: 2629 81 1401 378 4848
Fragments arranged by size:
4848 2629 1401 378 81
EcoRV GAT'ATC
Cuts at: 4481 4481
Size: 9337
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4615 5353 4615
Size: 738 8599
FspI TGC'GCA
Cuts at: 4446 7843 4446
Size: 3397 5940
HaeII r_GCGC'y
Cuts at: 1678 2048 8484 1678
Size: 370 6436 2531
Fragments arranged by size:
6436 2531 370
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3013 3045 3278 3310 3993
Size: 32 135 32 1441 32 233 32 683
Cuts at: 3993 4025 4781 4813 6137 6169 7978 8010 8052
Size: 32 756 32 1324 32 1809 32 42
Cuts at: 8052 8084 1373
Size: 32 2626
Fragments arranged by size:
2626 1809 1441 1324 756 683 233 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3805 4515 6681 6927 669
Size: 988 281 1867 710 2166 246 3079
Fragments arranged by size:
3079 2166 1867 988 710 281 246
HindIII A'AGCT_T
Cuts at: 748 3993 4250 4296 4779 748
Size: 3245 257 46 483 5306
Fragments arranged by size:
5306 3245 483 257 46
HpaI GTT'AAC
Cuts at: 3805 6927 3805
Size: 3122 6215
KpnI G_GTAC'C
Cuts at: 5624 6662 5624
Size: 1038 8299
MfeI C'AATT_G
Cuts at: 6936 6936
Size: 9337
MluI A'CGCG_T
Cuts at: 9213 9213
Size: 9337
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2816 5648 8451 8635 1305
Size: 1511 2832 2803 184 2007
Fragments arranged by size:
2832 2803 2007 1511 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3971 10
Size: 54 1425 343 2139 5376
Fragments arranged by size:
5376 2139 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2095 2227 3398 3543 6599 7295 7654
Size: 648 929 132 1171 145 3056 696 359
Cuts at: 7654 7813 518
Size: 159 2042
Fragments arranged by size:
3056 2042 1171 929 696 648 359 159
145 132
MspA1I CmG'CkG
Cuts at: 1606 2071 2118 4549 5561 6067 6470 6528 6753
Size: 465 47 2431 1012 506 403 58 225
Cuts at: 6753 7430 8578 8823 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2431 2120 1148 1012 677 506 465 403
245 225 58 47
NcoI C'CATG_G
Cuts at: 513 1065 2004 6022 513
Size: 552 939 4018 3828
Fragments arranged by size:
4018 3828 939 552
NdeI CA'TA_TG
Cuts at: 387 3201 387
Size: 2814 6523
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9337
NotI GC'GGCC_GC
Cuts at: 2198 6784 2198
Size: 4586 4751
NsiI A_TGCA'T
Cuts at: 4246 4246
Size: 9337
NspI r_CATG'y
Cuts at: 1132 1229 2232 2372 3193 3397 4101 6695 8240
Size: 97 1003 140 821 204 704 2594 1545
Cuts at: 8240 1132
Size: 2229
Fragments arranged by size:
2594 2229 1545 1003 821 704 204 140
97
PasI CC'CwG_GG
Cuts at: 1348 3572 1348
Size: 2224 7113
PciI A'CATG_T
Cuts at: 2368 8236 2368
Size: 5868 3469
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5061 5524 1108
Size: 3953 463 4921
Fragments arranged by size:
4921 3953 463
PmeI GTTT'AAAC
Cuts at: 6641 6641
Size: 9337
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2523 2555 7406 7438 8919 8951 2523
Size: 32 4851 32 1481 32 2909
Fragments arranged by size:
4851 2909 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 5896 1613
Size: 4283 5054
PsiI TTA'TAA
Cuts at: 4188 6907 4188
Size: 2719 6618
PspOMI G'GGCC_C
Cuts at: 2059 2065 2059
Size: 6 9331
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 5184 5216 5184
Size: 32 9305
PstI C_TGCA'G
Cuts at: 830 4677 4890 5088 5591 6486 6689 830
Size: 3847 213 198 503 895 203 3478
Fragments arranged by size:
3847 3478 895 503 213 203 198
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9337
PvuII CAG'CTG
Cuts at: 2118 4549 5561 6067 6470 6528 2118
Size: 2431 1012 506 403 58 4927
Fragments arranged by size:
4927 2431 1012 506 403 58
SacI G_AGCT'C
Cuts at: 4530 4530
Size: 9337
SalI G'TCGA_C
Cuts at: 1936 6679 1936
Size: 4743 4594
SapI GCTCTTCn'nnn_
Cuts at: 4563 4563
Size: 9337
SbfI CC_TGCA'GG
Cuts at: 5088 6689 5088
Size: 1601 7736
ScaI AGT'ACT
Cuts at: 2333 2353 7585 9297 2333
Size: 20 5232 1712 2373
Fragments arranged by size:
5232 2373 1712 20
SexAI A'CCwGG_T
Cuts at: 3557 3557
Size: 9337
SfcI C'TryA_G
Cuts at: 826 1045 4673 4886 5084 5587 6482 6685 7820
Size: 219 3628 213 198 503 895 203 1135
Cuts at: 7820 8501 8692 826
Size: 681 191 1471
Fragments arranged by size:
3628 1471 1135 895 681 503 219 213
203 198 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9337
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9337
SmaI CCC'GGG
Cuts at: 1872 2064 6664 1872
Size: 192 4600 4545
Fragments arranged by size:
4600 4545 192
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4940 6221 7441 8342 8604 820
Size: 197 926 2997 1281 1220 901 262 1553
Fragments arranged by size:
2997 1553 1281 1220 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 7128 493
Size: 6635 2702
SpeI A'CTAG_T
Cuts at: 152 3241 152
Size: 3089 6248
SphI G_CATG'C
Cuts at: 1132 6695 1132
Size: 5563 3774
SrfI GCCC'GGGC
Cuts at: 2064 2064
Size: 9337
SspI AAT'ATT
Cuts at: 5 52 2725 7261 5
Size: 47 2673 4536 2081
Fragments arranged by size:
4536 2673 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 9337
StyI C'CwwG_G
Cuts at: 513 1065 2004 3045 3458 3918 4834 6022 6333
Size: 552 939 1041 413 460 916 1188 311
Cuts at: 6333 6776 513
Size: 443 3074
Fragments arranged by size:
3074 1188 1041 939 916 552 460 443
413 311
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2043 6162 7364 7381 7534 7719 8164
Size: 1255 463 4119 1202 17 153 185 445
Cuts at: 8164 325
Size: 1498
Fragments arranged by size:
4119 1498 1255 1202 463 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2331 2351 2625
Size: 274 80 33 51 1443 354 20 274
Cuts at: 2625 6608 7583 9295 96
Size: 3983 975 1712 138
Fragments arranged by size:
3983 1712 1443 975 354 274 274 138
80 51 33 20
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5085 5117 5203 5235 6486 6518 9297 9329 5085
Size: 32 86 32 1251 32 2779 32 5093
Fragments arranged by size:
5093 2779 1251 86 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 6509 1316
Size: 5193 4144
XbaI T'CTAG_A
Cuts at: 6673 6673
Size: 9337
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2920 2920
Size: 9337
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 9337
XmaI C'CCGG_G
Cuts at: 1870 2062 6662 1870
Size: 192 4600 4545
Fragments arranged by size:
4600 4545 192
XmnI GAAnn'nnTTC
Cuts at: 2535 4373 4617 6347 6381 7466 2535
Size: 1838 244 1730 34 1085 4406
Fragments arranged by size:
4406 1838 1730 1085 244 34
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2777 4823 7145 276
Size: 53 83 186 936 1243 2046 2322 2468
Fragments arranged by size:
2468 2322 2046 1243 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PspOMI PsrI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BsiWI BstZ17I
DraIII FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspXI RsrII SacII SanDI
SfiI SfoI SwaI