Restriction map for
pFN21AE9633
(Circular) (Six-base) MAPSORT of: pFN21AE9633.seq Check: 1136 from: 1 to: 8713
pFN21AE9633 8713 bp
With 182 enzymes: SgfI *
January 16, 2014 19:09 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3403 3403
Size: 8713
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2388 6523 278
Size: 53 83 186 936 852 4135 2468
Fragments arranged by size:
4135 2468 936 852 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2583 2952 2964 3468 6056 1937
Size: 646 369 12 504 2588 4594
Fragments arranged by size:
4594 2588 646 504 369 12
Acc65I G'GTAC_C
Cuts at: 2757 6034 2757
Size: 3277 5436
AclI AA'CG_TT
Cuts at: 1563 6840 7213 1563
Size: 5277 373 3063
Fragments arranged by size:
5277 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2191 2645 2924 3566 3680 4978 5209 6776
Size: 559 454 279 642 114 1298 231 1567
Cuts at: 6776 8160 8428 1632
Size: 1384 268 1917
Fragments arranged by size:
1917 1567 1384 1298 642 559 454 279
268 231 114
AfeI AGC'GCT
Cuts at: 3134 3134
Size: 8713
AflII C'TTAA_G
Cuts at: 820 1017 4103 820
Size: 197 3086 5430
Fragments arranged by size:
5430 3086 197
AflIII A'CryG_T
Cuts at: 1203 2607 3114 7612 8589 1203
Size: 1404 507 4498 977 1327
Fragments arranged by size:
4498 1404 1327 977 507
AgeI A'CCGG_T
Cuts at: 7544 7544
Size: 8713
AhdI GACnn_n'nnGTC
Cuts at: 5381 7442 5381
Size: 2061 6652
AleI CACnn'nnGTG
Cuts at: 1166 3983 1166
Size: 2817 5896
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4308 1184
Size: 3124 5589
AlwNI CAG_nnn'CTG
Cuts at: 2623 4438 4533 5277 5745 5860 6456 8028 2623
Size: 1815 95 744 468 115 596 1572 3308
Fragments arranged by size:
3308 1815 1572 744 596 468 115 95
ApaI G_GGCC'C
Cuts at: 3776 3920 4784 5046 3776
Size: 144 864 262 7443
Fragments arranged by size:
7443 864 262 144
ApaLI G'TGCA_C
Cuts at: 2255 4397 6770 7926 2255
Size: 2142 2373 1156 3042
Fragments arranged by size:
3042 2373 2142 1156
ApoI r'AATT_y
Cuts at: 1493 2111 3062 6022 6248 6400 8509 1493
Size: 618 951 2960 226 152 2109 1697
Fragments arranged by size:
2960 2109 1697 951 618 226 152
AseI AT'TA_AT
Cuts at: 160 1032 7267 160
Size: 872 6235 1606
Fragments arranged by size:
6235 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8713
AvaI C'yCGr_G
Cuts at: 1870 1943 2492 3184 3374 3608 3920 4682 4697
Size: 73 549 692 190 234 312 762 15
Cuts at: 4697 4966 5019 5802 6038 1870
Size: 269 53 783 236 4545
Fragments arranged by size:
4545 783 762 692 549 312 269 236
234 190 73 53 15
BamHI G'GATC_C
Cuts at: 2940 6043 6410 2940
Size: 3103 367 5243
Fragments arranged by size:
5243 3103 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2757 3307 3828 4321 4357
Size: 325 217 627 970 550 521 493 36
Cuts at: 4357 4702 5012 5031 5792 6034 7489 618
Size: 345 310 19 761 242 1455 1842
Fragments arranged by size:
1842 1455 970 761 627 550 521 493
345 325 310 242 217 36 19
BanII G_rGCy'C
Cuts at: 1391 3776 3823 3920 4784 4866 4956 5007 5046
Size: 2385 47 97 864 82 90 51 39
Cuts at: 5046 5457 6032 1391
Size: 411 575 4072
Fragments arranged by size:
4072 2385 864 575 411 97 90 82
51 47 39
BbeI G_GCGC'C
Cuts at: 3832 4325 4706 3832
Size: 493 381 7839
Fragments arranged by size:
7839 493 381
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3734 4023 5215 928
Size: 971 1835 289 1192 4426
Fragments arranged by size:
4426 1835 1192 971 289
BbvCI CC'TCA_GC
Cuts at: 4526 5234 5396 4526
Size: 708 162 7843
Fragments arranged by size:
7843 708 162
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4339 4373 6904 6938 1903
Size: 34 2402 34 2531 34 3678
Fragments arranged by size:
3678 2531 2402 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5120 6605 7815 5120
Size: 1485 1210 6018
Fragments arranged by size:
6018 1485 1210
BclI T'GATC_A
Cuts at: 1548 3250 5851 1548
Size: 1702 2601 4410
Fragments arranged by size:
4410 2601 1702
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4663 4960 5667
Size: 107 122 71 631 605 2991 297 707
Cuts at: 5667 7324 136
Size: 1657 1525
Fragments arranged by size:
2991 1657 1525 707 631 605 297 122
107 71
BglII A'GATC_T
Cuts at: 8708 8708
Size: 8713
BlpI GC'TnA_GC
Cuts at: 4841 4853 5426 6130 4841
Size: 12 573 704 7424
Fragments arranged by size:
7424 704 573 12
Bme1580I G_kGCm'C
Cuts at: 1790 2259 2450 3012 3310 3776 3920 4401 4784
Size: 469 191 562 298 466 144 481 383
Cuts at: 4784 5046 6774 7930 1790
Size: 262 1728 1156 2573
Fragments arranged by size:
2573 1728 1156 562 481 469 466 383
298 262 191 144
BmgBI CAC'GTC
Cuts at: 1318 2179 2422 4396 5194 5755 1318
Size: 861 243 1974 798 561 4276
Fragments arranged by size:
4276 1974 861 798 561 243
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2354 2461 2605
Size: 416 53 83 399 24 937 107 144
Cuts at: 2605 3708 6444 7402 442
Size: 1103 2736 958 1753
Fragments arranged by size:
2736 1753 1103 958 937 416 399 144
107 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 8713
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2322 2324 4527 2322
Size: 2 2203 6508
Fragments arranged by size:
6508 2203 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2234 2573 3125 3248 3659 4723 5497 7373
Size: 995 339 552 123 411 1064 774 1876
Cuts at: 7373 1239
Size: 2579
Fragments arranged by size:
2579 1876 1064 995 774 552 411 339
123
Bpu10I CC'TnA_GC
Cuts at: 4526 5234 5396 4526
Size: 708 162 7843
Fragments arranged by size:
7843 708 162
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4232 6838 7703 8001 4232
Size: 2606 865 298 4944
Fragments arranged by size:
4944 2606 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2865 3514 5500 7376 882
Size: 617 1366 649 1986 1876 2219
Fragments arranged by size:
2219 1986 1876 1366 649 617
BsaAI yAC'GTr
Cuts at: 493 1204 2293 3115 6504 493
Size: 711 1089 822 3389 2702
Fragments arranged by size:
3389 2702 1089 822 711
BsaBI GATnn'nnATC
Cuts at: 8707 8707
Size: 8713
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2385 2489 3829 4322
Size: 53 83 186 936 852 104 1340 493
Cuts at: 4322 4703 6520 6902 7537 275
Size: 381 1817 382 635 1451
Fragments arranged by size:
1817 1451 1340 936 852 635 493 382
381 186 104 83 53
BsaWI w'CCGG_w
Cuts at: 2933 3804 5529 5640 7146 7544 7818 7965 8376
Size: 871 1725 111 1506 398 274 147 411
Cuts at: 8376 2933
Size: 3270
Fragments arranged by size:
3270 1725 1506 871 411 398 274 147
111
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2862 2892 3478 3508 4297
Size: 30 165 30 1459 30 586 30 789
Cuts at: 4297 4327 4531 4561 4721 4751 1178
Size: 30 204 30 160 30 5140
Fragments arranged by size:
5140 1459 789 586 204 165 160 30
30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2198 2231 2843 3251 3254 3281 3284 3287
Size: 1012 33 612 408 3 27 3 3
Cuts at: 3287 3299 4046 4318 4331 4477 4802 4964 1186
Size: 12 747 272 13 146 325 162 4935
Fragments arranged by size:
4935 1012 747 612 408 325 272 162
146 33 27 13 12 3 3 3
BseYI C'CCAG_C
Cuts at: 2499 2616 3129 3194 3769 4152 4760 5038 5045
Size: 117 513 65 575 383 608 278 7
Cuts at: 5045 7916 8412 2499
Size: 2871 496 2800
Fragments arranged by size:
2871 2800 608 575 513 496 383 278
117 65 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2792 2978 3074 1163
Size: 573 1056 186 96 6802
Fragments arranged by size:
6802 1056 573 186 96
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 6163 6924 7952 1540
Size: 275 186 4162 761 1028 2301
Fragments arranged by size:
4162 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2259 2559 3669 3823 4070 4347 4401 4866 6774
Size: 300 1110 154 247 277 54 465 1908
Cuts at: 6774 6859 7930 2259
Size: 85 1071 3042
Fragments arranged by size:
3042 1908 1110 1071 465 300 277 247
154 85 54
BsmI GAATG_Cn'
Cuts at: 6224 6317 6224
Size: 93 8620
BsmBI CGTCTCn'nnnn_
Cuts at: 2376 2480 5389 2376
Size: 104 2909 5700
Fragments arranged by size:
5700 2909 104
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2259 2450 2559 3012 3310 3669 3776
Size: 399 469 191 109 453 298 359 107
Cuts at: 3776 3823 3920 4070 4347 4401 4784 4866 4956
Size: 47 97 150 277 54 383 82 90
Cuts at: 4956 5007 5046 5457 6032 6774 6859 7930 1391
Size: 51 39 411 575 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 575 469 453 411 399
383 359 298 277 191 150 109 107
97 90 85 82 54 51 47 39
BspEI T'CCGG_A
Cuts at: 5529 5640 8376 5529
Size: 111 2736 5866
Fragments arranged by size:
5866 2736 111
BspHI T'CATG_A
Cuts at: 6602 8332 6602
Size: 1730 6983
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3403 4239 4300 4323 5784 6068 844
Size: 2559 836 61 23 1461 284 3489
Fragments arranged by size:
3489 2559 1461 836 284 61 23
BsrBI CCG'CTC
Cuts at: 1394 1823 4737 6600 1394
Size: 429 2914 1863 3507
Fragments arranged by size:
3507 2914 1863 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2655 4084 7208 7382 65
Size: 1183 1407 1429 3124 174 1396
Fragments arranged by size:
3124 1429 1407 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2406 3488 4178 5442 7357 7544 1700
Size: 110 596 1082 690 1264 1915 187 2869
Fragments arranged by size:
2869 1915 1264 1082 690 596 187 110
BsrGI T'GTAC_A
Cuts at: 96 2514 96
Size: 2418 6295
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8713
BssSI C'ACGA_G
Cuts at: 4871 6773 7785 4871
Size: 1902 1012 5799
Fragments arranged by size:
5799 1902 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8713
BstBI TT'CG_AA
Cuts at: 7517 7517
Size: 8713
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8713
BstXI CCAn_nnnn'nTGG
Cuts at: 3460 4152 3460
Size: 692 8021
BstYI r'GATC_y
Cuts at: 1972 2027 2940 3024 4541 4875 5581 6043 6410
Size: 55 913 84 1517 334 706 462 367
Cuts at: 6410 6795 6812 8264 8350 8362 8708 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1517 1452 913 706 462 385 367
346 334 86 84 55 17 12
Bsu36I CC'TnA_GG
Cuts at: 3261 4403 5903 3261
Size: 1142 1500 6071
Fragments arranged by size:
6071 1500 1142
BtgI C'CryG_G
Cuts at: 513 1065 2004 2200 2262 3501 3783 4752 513
Size: 552 939 196 62 1239 282 969 4474
Fragments arranged by size:
4474 1239 969 939 552 282 196 62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3853 3943 4446 487
Size: 683 56 2627 90 503 4754
Fragments arranged by size:
4754 2627 683 503 90 56
BtsI GCAGTG_nn'
Cuts at: 3959 5329 5781 6229 7023 7043 3959
Size: 1370 452 448 794 20 5629
Fragments arranged by size:
5629 1370 794 452 448 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8713
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 5860 5895 529
Size: 35 5296 35 3347
Fragments arranged by size:
5296 3347 35 35
DraI TTT'AAA
Cuts at: 6017 6017
Size: 8713
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7720 809
Size: 507 6404 1802
Fragments arranged by size:
6404 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2121 3462 3690
Size: 54 1425 50 275 18 291 1341 228
Cuts at: 3690 3719 3795 4647 4899 5135 5217 6160 7049
Size: 29 76 852 252 236 82 943 889
Cuts at: 7049 8678 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1341 943 889 852 291 275
252 236 228 82 76 54 50 43
29 18
EagI C'GGCC_G
Cuts at: 1537 1812 6160 1537
Size: 275 4348 4090
Fragments arranged by size:
4348 4090 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3217 4023 6643 1353
Size: 1864 806 2620 3423
Fragments arranged by size:
3423 2620 1864 806
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2997 5696 7300 7674 7820 387
Size: 1064 138 1408 2699 1604 374 146 1280
Fragments arranged by size:
2699 1604 1408 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3821 4864 3821
Size: 1043 7670
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2191 2234 2573 2645 2924 3125 3248
Size: 393 559 43 339 72 279 201 123
Cuts at: 3248 3566 3659 3680 4723 4978 5209 5497 6776
Size: 318 93 21 1043 255 231 288 1279
Cuts at: 6776 7373 8160 8428 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1279 1043 787 597 559 393 339
318 288 279 268 255 231 201 123
93 72 43 21
EcoNI CCTnn'n_nnAGG
Cuts at: 3146 4247 3146
Size: 1101 7612
EcoO109I rG'GnC_Cy
Cuts at: 3370 3550 3622 3703 3916 3917 4506 4757 4780
Size: 180 72 81 213 1 589 251 23
Cuts at: 4780 5042 5274 3370
Size: 262 232 6809
Fragments arranged by size:
6809 589 262 251 232 213 180 81
72 23 1
EcoRI G'AATT_C
Cuts at: 6022 6400 6022
Size: 378 8335
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 4610 4610
Size: 8713
FspI TGC'GCA
Cuts at: 4379 5054 7219 4379
Size: 675 2165 5873
Fragments arranged by size:
5873 2165 675
HaeII r_GCGC'y
Cuts at: 1678 2134 2822 3136 3832 4325 4646 4669 4706
Size: 456 688 314 696 493 321 23 37
Cuts at: 4706 5125 7860 1678
Size: 419 2735 2531
Fragments arranged by size:
2735 2531 696 688 493 456 419 321
314 37 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2156 2188 3194 3226 4331
Size: 32 135 32 584 32 1006 32 1105
Cuts at: 4331 4363 4531 4533 4563 4565 5367 5399 5573
Size: 32 168 2 30 2 802 32 174
Cuts at: 5573 5605 5611 5643 7354 7386 7428 7460 1373
Size: 32 6 32 1711 32 42 32 2626
Fragments arranged by size:
2626 1711 1105 1006 802 584 174 168
135 42 32 32 32 32 32 32
32 32 32 32 30 6 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 4132 6057 6303 669
Size: 988 281 2194 1925 246 3079
Fragments arranged by size:
3079 2194 1925 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2222 4592 748
Size: 1474 2370 4869
Fragments arranged by size:
4869 2370 1474
HpaI GTT'AAC
Cuts at: 6303 6303
Size: 8713
KasI G'GCGC_C
Cuts at: 3828 4321 4702 3828
Size: 493 381 7839
Fragments arranged by size:
7839 493 381
KpnI G_GTAC'C
Cuts at: 2761 6038 2761
Size: 3277 5436
MfeI C'AATT_G
Cuts at: 6312 6312
Size: 8713
MluI A'CGCG_T
Cuts at: 8589 8589
Size: 8713
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2154 2597 2825 2918 3414 4251 4317 4908
Size: 849 443 228 93 496 837 66 591
Cuts at: 4908 7827 8011 1305
Size: 2919 184 2007
Fragments arranged by size:
2919 2007 849 837 591 496 443 228
184 93 66
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3692 3721 4649 4901 10
Size: 54 1425 343 1860 29 928 252 3822
Fragments arranged by size:
3822 1860 1425 928 343 252 54 29
MslI CAynn'nnrTG
Cuts at: 518 1166 2453 3672 3983 4058 6671 7030 7189
Size: 648 1287 1219 311 75 2613 359 159
Cuts at: 7189 518
Size: 2042
Fragments arranged by size:
2613 2042 1287 1219 648 359 311 159
75
MspA1I CmG'CkG
Cuts at: 1606 2202 2236 2503 3019 3785 4754 5038 5286
Size: 596 34 267 516 766 969 284 248
Cuts at: 5286 5400 5425 5524 6129 6806 7954 8199 1606
Size: 114 25 99 605 677 1148 245 2120
Fragments arranged by size:
2120 1148 969 766 677 605 596 516
284 267 248 245 114 99 34 25
NaeI GCC'GGC
Cuts at: 2408 4180 2408
Size: 1772 6941
NarI GG'CG_CC
Cuts at: 3829 4322 4703 3829
Size: 493 381 7839
Fragments arranged by size:
7839 493 381
NcoI C'CATG_G
Cuts at: 513 1065 2004 2262 513
Size: 552 939 258 6964
Fragments arranged by size:
6964 939 552 258
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8713
NgoMIV G'CCGG_C
Cuts at: 2406 4178 2406
Size: 1772 6941
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 8713
NotI GC'GGCC_GC
Cuts at: 6160 6160
Size: 8713
NspI r_CATG'y
Cuts at: 1132 1229 2063 2464 2611 5263 5716 6071 7616
Size: 97 834 401 147 2652 453 355 1545
Cuts at: 7616 1132
Size: 2229
Fragments arranged by size:
2652 2229 1545 834 453 401 355 147
97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8713
PciI A'CATG_T
Cuts at: 2607 7612 2607
Size: 5005 3708
PflMI CCAn_nnn'nTGG
Cuts at: 1108 5551 1108
Size: 4443 4270
PfoI T'CCnGG_A
Cuts at: 3102 3585 3636 4616 5457 3102
Size: 483 51 980 841 6358
Fragments arranged by size:
6358 980 841 483 51
PmeI GTTT'AAAC
Cuts at: 6017 6017
Size: 8713
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6782 6814 8295 8327 6782
Size: 32 1481 32 7168
Fragments arranged by size:
7168 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3622 3703 4757 5274 3622
Size: 81 1054 517 7061
Fragments arranged by size:
7061 1054 517 81
PshAI GACnn'nnGTC
Cuts at: 1613 3684 1613
Size: 2071 6642
PsiI TTA'TAA
Cuts at: 6283 6283
Size: 8713
PspOMI G'GGCC_C
Cuts at: 3772 3916 4780 5042 3772
Size: 144 864 262 7443
Fragments arranged by size:
7443 864 262 144
PstI C_TGCA'G
Cuts at: 830 2035 5273 6065 830
Size: 1205 3238 792 3478
Fragments arranged by size:
3478 3238 1205 792
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8713
PvuII CAG'CTG
Cuts at: 2236 3019 5038 5400 5425 2236
Size: 783 2019 362 25 5524
Fragments arranged by size:
5524 2019 783 362 25
RsrII CG'GwC_CG
Cuts at: 3867 3867
Size: 8713
SacI G_AGCT'C
Cuts at: 3823 4866 3823
Size: 1043 7670
SacII CC_GC'GG
Cuts at: 2203 3786 4755 2203
Size: 1583 969 6161
Fragments arranged by size:
6161 1583 969
SalI G'TCGA_C
Cuts at: 1936 6055 1936
Size: 4119 4594
SanDI GG'GwC_CC
Cuts at: 3703 4757 3703
Size: 1054 7659
SbfI CC_TGCA'GG
Cuts at: 6065 6065
Size: 8713
ScaI AGT'ACT
Cuts at: 2690 6961 8673 2690
Size: 4271 1712 2730
Fragments arranged by size:
4271 2730 1712
SexAI A'CCwGG_T
Cuts at: 2586 2586
Size: 8713
SfcI C'TryA_G
Cuts at: 826 1045 2031 2961 5269 6061 7196 7877 8068
Size: 219 986 930 2308 792 1135 681 191
Cuts at: 8068 826
Size: 1471
Fragments arranged by size:
2308 1471 1135 986 930 792 681 219
191
SfoI GGC'GCC
Cuts at: 3830 4323 4704 3830
Size: 493 381 7839
Fragments arranged by size:
7839 493 381
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8713
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8713
SmaI CCC'GGG
Cuts at: 1872 3922 4968 5021 6040 1872
Size: 2050 1046 53 1019 4545
Fragments arranged by size:
4545 2050 1046 1019 53
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4103 4247 6817 7718 7980 820
Size: 197 926 2160 144 2570 901 262 1553
Fragments arranged by size:
2570 2160 1553 926 901 262 197 144
SnaBI TAC'GTA
Cuts at: 493 2293 6504 493
Size: 1800 4211 2702
Fragments arranged by size:
4211 2702 1800
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8713
SphI G_CATG'C
Cuts at: 1132 5263 6071 1132
Size: 4131 808 3774
Fragments arranged by size:
4131 3774 808
SspI AAT'ATT
Cuts at: 5 52 6637 5
Size: 47 6585 2081
Fragments arranged by size:
6585 2081 47
StuI AGG'CCT
Cuts at: 1517 3245 3857 5670 1517
Size: 1728 612 1813 4560
Fragments arranged by size:
4560 1813 1728 612
StyI C'CwwG_G
Cuts at: 513 1065 2004 2262 3759 5925 6152 513
Size: 552 939 258 1497 2166 227 3074
Fragments arranged by size:
3074 2166 1497 939 552 258 227
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2453 2587 2824 3325 3355 3884 5539
Size: 1255 873 134 237 501 30 529 1655
Cuts at: 5539 6740 6757 6910 7095 7540 325
Size: 1201 17 153 185 445 1498
Fragments arranged by size:
1655 1498 1255 1201 873 529 501 445
237 185 153 134 30 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2514 2688 3117
Size: 274 80 33 51 1443 537 174 429
Cuts at: 3117 6959 8671 96
Size: 3842 1712 138
Fragments arranged by size:
3842 1712 1443 537 429 274 174 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2594 2626 2862 2894 3478 3510 4297 4329 4721
Size: 32 236 32 584 32 787 32 392
Cuts at: 4721 4753 5531 5563 8673 8705 2594
Size: 32 778 32 3110 32 2602
Fragments arranged by size:
3110 2602 787 778 584 392 236 32
32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3421 1316
Size: 2105 6608
XbaI T'CTAG_A
Cuts at: 6049 6049
Size: 8713
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2735 3766 4439 2735
Size: 1031 673 7009
Fragments arranged by size:
7009 1031 673
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8713
XmaI C'CCGG_G
Cuts at: 1870 3920 4966 5019 6038 1870
Size: 2050 1046 53 1019 4545
Fragments arranged by size:
4545 2050 1046 1019 53
XmnI GAAnn'nnTTC
Cuts at: 3908 6842 3908
Size: 2934 5779
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2386 6521 276
Size: 53 83 186 936 852 4135 2468
Fragments arranged by size:
4135 2468 936 852 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII
EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI
PsrI SapI SfiI SrfI SwaI