Restriction map for
pFN21AE9630
(Circular) (Six-base) MAPSORT of: pFN21AE9630.seq Check: 9906 from: 1 to: 7960
pFN21AE9630 7960 bp
With 182 enzymes: SgfI *
July 6, 2012 17:34 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4043 4043
Size: 7960
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5770 278
Size: 53 83 186 936 4234 2468
Fragments arranged by size:
4234 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3827 5303 1937
Size: 1890 1476 4594
Fragments arranged by size:
4594 1890 1476
Acc65I G'GTAC_C
Cuts at: 5281 5281
Size: 7960
AclI AA'CG_TT
Cuts at: 1563 6087 6460 1563
Size: 4524 373 3063
Fragments arranged by size:
4524 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2012 2354 2583 3741 5070 5186 6023 7407
Size: 380 342 229 1158 1329 116 837 1384
Cuts at: 7407 7675 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1329 1158 837 380 342 268
229 116
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7763
AflIII A'CryG_T
Cuts at: 1203 4011 4545 6859 7836 1203
Size: 2808 534 2314 977 1327
Fragments arranged by size:
2808 2314 1327 977 534
AgeI A'CCGG_T
Cuts at: 6791 6791
Size: 7960
AhdI GACnn_n'nnGTC
Cuts at: 6689 6689
Size: 7960
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7960
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2376 1184
Size: 1192 6768
AlwNI CAG_nnn'CTG
Cuts at: 3007 4112 5703 7275 3007
Size: 1105 1591 1572 3692
Fragments arranged by size:
3692 1591 1572 1105
ApaI G_GGCC'C
Cuts at: 5209 5209
Size: 7960
ApaLI G'TGCA_C
Cuts at: 2666 3389 3860 6017 7173 2666
Size: 723 471 2157 1156 3453
Fragments arranged by size:
3453 2157 1156 723 471
ApoI r'AATT_y
Cuts at: 1493 2113 2168 2225 2379 2747 3245 3962 4340
Size: 620 55 57 154 368 498 717 378
Cuts at: 4340 4454 4493 5093 5269 5495 5647 7756 1493
Size: 114 39 600 176 226 152 2109 1697
Fragments arranged by size:
2109 1697 717 620 600 498 378 368
226 176 154 152 114 57 55 39
AseI AT'TA_AT
Cuts at: 160 1032 6514 160
Size: 872 5482 1606
Fragments arranged by size:
5482 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7960
AvaI C'yCGr_G
Cuts at: 1870 1943 5285 1870
Size: 73 3342 4545
Fragments arranged by size:
4545 3342 73
BamHI G'GATC_C
Cuts at: 5290 5657 5290
Size: 367 7593
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3076 3775 3943 5281 6736
Size: 325 217 627 1289 699 168 1338 1455
Cuts at: 6736 618
Size: 1842
Fragments arranged by size:
1842 1455 1338 1289 699 627 325 217
168
BanII G_rGCy'C
Cuts at: 1391 2773 3794 4158 4608 5209 5279 1391
Size: 1382 1021 364 450 601 70 4072
Fragments arranged by size:
4072 1382 1021 601 450 364 70
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2823 3014 928
Size: 971 924 191 5874
Fragments arranged by size:
5874 971 924 191
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6151 6185 1903
Size: 34 4214 34 3678
Fragments arranged by size:
4214 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5852 7062 5852
Size: 1210 6750
BclI T'GATC_A
Cuts at: 1548 2637 1548
Size: 1089 6871
BfrBI ATG'CAT
Cuts at: 3500 3500
Size: 7960
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6571 136
Size: 107 122 71 631 605 4899 1525
Fragments arranged by size:
4899 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 4426 7955 4426
Size: 3529 4431
BlpI GC'TnA_GC
Cuts at: 4934 5377 4934
Size: 443 7517
Bme1580I G_kGCm'C
Cuts at: 1790 2670 3393 3864 5209 6021 7177 1790
Size: 880 723 471 1345 812 1156 2573
Fragments arranged by size:
2573 1345 1156 880 812 723 471
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7960
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3350 3505 3774
Size: 416 53 83 399 24 1933 155 269
Cuts at: 3774 4504 5691 6649 442
Size: 730 1187 958 1753
Fragments arranged by size:
1933 1753 1187 958 730 416 399 269
155 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7960
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3168 3168
Size: 7960
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2607 6620 1239
Size: 1368 4013 2579
Fragments arranged by size:
4013 2579 1368
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2451 4659 6085 6950 7248 2451
Size: 2208 1426 865 298 3163
Fragments arranged by size:
3163 2208 1426 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 6623 882
Size: 617 5124 2219
Fragments arranged by size:
5124 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2975 5213 5751 493
Size: 711 1771 2238 538 2702
Fragments arranged by size:
2702 2238 1771 711 538
BsaBI GATnn'nnATC
Cuts at: 5127 7954 5127
Size: 2827 5133
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5767 6149 6784 275
Size: 53 83 186 936 4234 382 635 1451
Fragments arranged by size:
4234 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 6393 6791 7065 7212 7623 6393
Size: 398 274 147 411 6730
Fragments arranged by size:
6730 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 7735
Fragments arranged by size:
7735 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3144 5049 1186
Size: 1958 1905 4097
Fragments arranged by size:
4097 1958 1905
BseYI C'CCAG_C
Cuts at: 2997 3130 4151 7163 7659 2997
Size: 133 1021 3012 496 3298
Fragments arranged by size:
3298 3012 1021 496 133
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3392 3798 1163
Size: 573 1656 406 5325
Fragments arranged by size:
5325 1656 573 406
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5410 6171 7199 1540
Size: 275 186 3409 761 1028 2301
Fragments arranged by size:
3409 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2670 3393 3864 4608 6021 6106 7177 2670
Size: 723 471 744 1413 85 1071 3453
Fragments arranged by size:
3453 1413 1071 744 723 471 85
BsmI GAATG_Cn'
Cuts at: 4710 5471 5564 4710
Size: 761 93 7106
Fragments arranged by size:
7106 761 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4011 4011
Size: 7960
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2670 2773 3393 3794 3864 4158 4608
Size: 399 880 103 620 401 70 294 450
Cuts at: 4608 5209 5279 6021 6106 7177 1391
Size: 601 70 742 85 1071 2174
Fragments arranged by size:
2174 1071 880 742 620 601 450 401
399 294 103 85 70 70
BspEI T'CCGG_A
Cuts at: 7623 7623
Size: 7960
BspHI T'CATG_A
Cuts at: 3241 3417 5849 7579 3241
Size: 176 2432 1730 3622
Fragments arranged by size:
3622 2432 1730 176
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2692 4043 5315 844
Size: 1848 1351 1272 3489
Fragments arranged by size:
3489 1848 1351 1272
BsrBI CCG'CTC
Cuts at: 1394 1823 5847 1394
Size: 429 4024 3507
Fragments arranged by size:
4024 3507 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2071 2310 2810 3147 4122 4313 6455
Size: 1183 823 239 500 337 975 191 2142
Cuts at: 6455 6629 65
Size: 174 1396
Fragments arranged by size:
2142 1396 1183 975 823 500 337 239
191 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 6604 6791 1700
Size: 110 4794 187 2869
Fragments arranged by size:
4794 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2271 2307 96
Size: 2175 36 5749
Fragments arranged by size:
5749 2175 36
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7960
BssSI C'ACGA_G
Cuts at: 6020 7032 6020
Size: 1012 6948
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3821 794
Size: 3027 4933
BstBI TT'CG_AA
Cuts at: 6764 6764
Size: 7960
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7960
BstXI CCAn_nnnn'nTGG
Cuts at: 2285 2285
Size: 7960
BstYI r'GATC_y
Cuts at: 1972 2932 4132 4426 5290 5657 6042 6059 7511
Size: 960 1200 294 864 367 385 17 1452
Cuts at: 7511 7597 7609 7955 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1200 960 864 385 367 346
294 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2278 4226 4946 513
Size: 552 939 274 1948 720 3527
Fragments arranged by size:
3527 1948 939 720 552 274
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 7221
Fragments arranged by size:
7221 683 56
BtsI GCAGTG_nn'
Cuts at: 3207 4594 5476 6270 6290 3207
Size: 1387 882 794 20 4877
Fragments arranged by size:
4877 1387 882 794 20
ClaI AT'CG_AT
Cuts at: 1554 4962 1554
Size: 3408 4552
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7925
DraI TTT'AAA
Cuts at: 2223 2508 4464 5264 2223
Size: 285 1956 800 4919
Fragments arranged by size:
4919 1956 800 285
DraIII CAC_nnn'GTG
Cuts at: 3260 3260
Size: 7960
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6967 809
Size: 507 5651 1802
Fragments arranged by size:
5651 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3204 5407 6296
Size: 54 1425 50 275 18 1374 2203 889
Cuts at: 6296 7925 8
Size: 1629 43
Fragments arranged by size:
2203 1629 1425 1374 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5407 1537
Size: 275 3595 4090
Fragments arranged by size:
4090 3595 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2103 2626 3571 3717 5890 1353
Size: 750 523 945 146 2173 3423
Fragments arranged by size:
3423 2173 945 750 523 146
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6547 6921 7067 387
Size: 1064 138 4958 374 146 1280
Fragments arranged by size:
4958 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 4606 4606
Size: 7960
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2012 2354 2583 2607 3741 5070 5186
Size: 393 380 342 229 24 1134 1329 116
Cuts at: 5186 6023 6620 7407 7675 1239
Size: 837 597 787 268 1524
Fragments arranged by size:
1524 1329 1134 837 787 597 393 380
342 268 229 116 24
EcoNI CCTnn'n_nnAGG
Cuts at: 4050 4050
Size: 7960
EcoO109I rG'GnC_Cy
Cuts at: 3657 4506 5205 5206 3657
Size: 849 699 1 6411
Fragments arranged by size:
6411 849 699 1
EcoRI G'AATT_C
Cuts at: 2379 3245 4493 5269 5647 2379
Size: 866 1248 776 378 4692
Fragments arranged by size:
4692 1248 866 776 378
EcoRV GAT'ATC
Cuts at: 2595 2595
Size: 7960
FspI TGC'GCA
Cuts at: 6466 6466
Size: 7960
HaeII r_GCGC'y
Cuts at: 1678 3017 7107 1678
Size: 1339 4090 2531
Fragments arranged by size:
4090 2531 1339
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2600 2632 3255 3287 3791
Size: 32 135 32 1028 32 623 32 504
Cuts at: 3791 3823 4270 4302 4802 4834 6601 6633 6675
Size: 32 447 32 500 32 1767 32 42
Cuts at: 6675 6707 1373
Size: 32 2626
Fragments arranged by size:
2626 1767 1028 623 504 500 447 135
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5304 5550 669
Size: 988 281 3366 246 3079
Fragments arranged by size:
3366 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2726 3842 748
Size: 1978 1116 4866
Fragments arranged by size:
4866 1978 1116
HpaI GTT'AAC
Cuts at: 5550 5550
Size: 7960
KpnI G_GTAC'C
Cuts at: 5285 5285
Size: 7960
MfeI C'AATT_G
Cuts at: 4346 5559 4346
Size: 1213 6747
MluI A'CGCG_T
Cuts at: 7836 7836
Size: 7960
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 7074 7258 1305
Size: 5769 184 2007
Fragments arranged by size:
5769 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3206 10
Size: 54 1425 343 1374 4764
Fragments arranged by size:
4764 1425 1374 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2358 3422 3941 4550 4734 5012 5018
Size: 648 1192 1064 519 609 184 278 6
Cuts at: 5018 5918 6277 6436 518
Size: 900 359 159 2042
Fragments arranged by size:
2042 1192 1064 900 648 609 519 359
278 184 159 6
MspA1I CmG'CkG
Cuts at: 1606 5376 6053 7201 7446 1606
Size: 3770 677 1148 245 2120
Fragments arranged by size:
3770 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2278 4226 4946 513
Size: 552 939 274 1948 720 3527
Fragments arranged by size:
3527 1948 939 720 552 274
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7960
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7960
NotI GC'GGCC_GC
Cuts at: 5407 5407
Size: 7960
NsiI A_TGCA'T
Cuts at: 3502 3502
Size: 7960
NspI r_CATG'y
Cuts at: 1132 1229 2050 2357 4015 4549 4999 5318 6863
Size: 97 821 307 1658 534 450 319 1545
Cuts at: 6863 1132
Size: 2229
Fragments arranged by size:
2229 1658 1545 821 534 450 319 307
97
PasI CC'CwG_GG
Cuts at: 1348 4420 4488 1348
Size: 3072 68 4820
Fragments arranged by size:
4820 3072 68
PciI A'CATG_T
Cuts at: 4011 4545 6859 4011
Size: 534 2314 5112
Fragments arranged by size:
5112 2314 534
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3260 4488 4516 1108
Size: 2152 1228 28 4552
Fragments arranged by size:
4552 2152 1228 28
PfoI T'CCnGG_A
Cuts at: 2536 2536
Size: 7960
PmeI GTTT'AAAC
Cuts at: 5264 5264
Size: 7960
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6029 6061 7542 7574 6029
Size: 32 1481 32 6415
Fragments arranged by size:
6415 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3657 4506 3657
Size: 849 7111
PshAI GACnn'nnGTC
Cuts at: 1613 4845 1613
Size: 3232 4728
PsiI TTA'TAA
Cuts at: 5530 5530
Size: 7960
PspOMI G'GGCC_C
Cuts at: 5205 5205
Size: 7960
PstI C_TGCA'G
Cuts at: 830 2352 3118 4054 5312 830
Size: 1522 766 936 1258 3478
Fragments arranged by size:
3478 1522 1258 936 766
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7960
SacI G_AGCT'C
Cuts at: 4608 4608
Size: 7960
SalI G'TCGA_C
Cuts at: 1936 5302 1936
Size: 3366 4594
SanDI GG'GwC_CC
Cuts at: 4506 4506
Size: 7960
SbfI CC_TGCA'GG
Cuts at: 5312 5312
Size: 7960
ScaI AGT'ACT
Cuts at: 3238 6208 7920 3238
Size: 2970 1712 3278
Fragments arranged by size:
3278 2970 1712
SfcI C'TryA_G
Cuts at: 826 1045 2348 3114 4050 4609 5308 6443 7124
Size: 219 1303 766 936 559 699 1135 681
Cuts at: 7124 7315 826
Size: 191 1471
Fragments arranged by size:
1471 1303 1135 936 766 699 681 559
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7960
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7960
SmaI CCC'GGG
Cuts at: 1872 5287 1872
Size: 3415 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2466 4674 6064 6965 7227 820
Size: 197 926 523 2208 1390 901 262 1553
Fragments arranged by size:
2208 1553 1390 926 901 523 262 197
SnaBI TAC'GTA
Cuts at: 493 5213 5751 493
Size: 4720 538 2702
Fragments arranged by size:
4720 2702 538
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7960
SphI G_CATG'C
Cuts at: 1132 2357 4999 5318 1132
Size: 1225 2642 319 3774
Fragments arranged by size:
3774 2642 1225 319
SspI AAT'ATT
Cuts at: 5 52 2660 5116 5884 5
Size: 47 2608 2456 768 2081
Fragments arranged by size:
2608 2456 2081 768 47
StuI AGG'CCT
Cuts at: 1517 3998 1517
Size: 2481 5479
StyI C'CwwG_G
Cuts at: 513 1065 2004 2278 2338 4226 4946 5399 513
Size: 552 939 274 60 1888 720 453 3074
Fragments arranged by size:
3074 1888 939 720 552 453 274 60
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3397 3793 5987 6004 6157 6342 6787
Size: 1255 1817 396 2194 17 153 185 445
Cuts at: 6787 325
Size: 1498
Fragments arranged by size:
2194 1817 1498 1255 445 396 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2235 2271 2307
Size: 274 80 33 51 1443 258 36 36
Cuts at: 2307 3236 5227 6206 7918 96
Size: 929 1991 979 1712 138
Fragments arranged by size:
1991 1712 1443 979 929 274 258 138
80 51 36 36 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7920 7952 7920
Size: 32 7928
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7960
XbaI T'CTAG_A
Cuts at: 3481 5296 3481
Size: 1815 6145
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3199 4379 3199
Size: 1180 6780
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7960
XmaI C'CCGG_G
Cuts at: 1870 5285 1870
Size: 3415 4545
XmnI GAAnn'nnTTC
Cuts at: 4743 5097 6089 4743
Size: 354 992 6614
Fragments arranged by size:
6614 992 354
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5768 276
Size: 53 83 186 936 4234 2468
Fragments arranged by size:
4234 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI SacI SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI Bpu10I
BsiWI BstZ17I Bsu36I FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PmlI PspXI PsrI PvuII
RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI