Restriction map for
pFN21AE9627
(Circular) (Six-base) MAPSORT of: pFN21AE9627.seq Check: 3086 from: 1 to: 7288
pFN21AE9627 7288 bp
With 182 enzymes: SgfI *
July 6, 2012 17:34 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5098 278
Size: 53 83 186 936 3562 2468
Fragments arranged by size:
3562 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4631 1937
Size: 2694 4594
Acc65I G'GTAC_C
Cuts at: 4609 4609
Size: 7288
AclI AA'CG_TT
Cuts at: 1563 5415 5788 1563
Size: 3852 373 3063
Fragments arranged by size:
3852 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2310 2562 2759 3039 3639 3893 4256 4433
Size: 678 252 197 280 600 254 363 177
Cuts at: 4433 5351 6735 7003 1632
Size: 918 1384 268 1917
Fragments arranged by size:
1917 1384 918 678 600 363 280 268
254 252 197 177
AfeI AGC'GCT
Cuts at: 4433 4433
Size: 7288
AflII C'TTAA_G
Cuts at: 820 1017 3628 820
Size: 197 2611 4480
Fragments arranged by size:
4480 2611 197
AflIII A'CryG_T
Cuts at: 1203 6187 7164 1203
Size: 4984 977 1327
Fragments arranged by size:
4984 1327 977
AgeI A'CCGG_T
Cuts at: 6119 6119
Size: 7288
AhdI GACnn_n'nnGTC
Cuts at: 2373 6017 2373
Size: 3644 3644
AleI CACnn'nnGTG
Cuts at: 1166 3396 1166
Size: 2230 5058
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7288
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2837 2869 2837
Size: 32 7256
AlwNI CAG_nnn'CTG
Cuts at: 3140 4015 5031 6603 3140
Size: 875 1016 1572 3825
Fragments arranged by size:
3825 1572 1016 875
ApaI G_GGCC'C
Cuts at: 2044 3119 2044
Size: 1075 6213
ApaLI G'TGCA_C
Cuts at: 3456 5345 6501 3456
Size: 1889 1156 4243
Fragments arranged by size:
4243 1889 1156
ApoI r'AATT_y
Cuts at: 1493 2674 2885 3369 4597 4823 4975 7084 1493
Size: 1181 211 484 1228 226 152 2109 1697
Fragments arranged by size:
2109 1697 1228 1181 484 226 211 152
AseI AT'TA_AT
Cuts at: 160 1032 3464 5842 160
Size: 872 2432 2378 1606
Fragments arranged by size:
2432 2378 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7288
AvaI C'yCGr_G
Cuts at: 1870 1943 2019 4613 1870
Size: 73 76 2594 4545
Fragments arranged by size:
4545 2594 76 73
AvrII C'CTAG_G
Cuts at: 2108 2108
Size: 7288
BamHI G'GATC_C
Cuts at: 4618 4985 4618
Size: 367 6921
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4609 6064 618
Size: 325 217 627 2822 1455 1842
Fragments arranged by size:
2822 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2020 2044 2075 3119 3162 3852 4029 4607
Size: 629 24 31 1044 43 690 177 578
Cuts at: 4607 1391
Size: 4072
Fragments arranged by size:
4072 1044 690 629 578 177 43 31
24
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2440 4273 928
Size: 971 541 1833 3943
Fragments arranged by size:
3943 1833 971 541
BbvCI CC'TCA_GC
Cuts at: 2115 2115
Size: 7288
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5479 5513 1903
Size: 34 3542 34 3678
Fragments arranged by size:
3678 3542 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5180 6390 5180
Size: 1210 6078
BclI T'GATC_A
Cuts at: 1548 1548
Size: 7288
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5899 136
Size: 107 122 71 631 605 4227 1525
Fragments arranged by size:
4227 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2467 7283 2467
Size: 4816 2472
BlpI GC'TnA_GC
Cuts at: 4705 4705
Size: 7288
Bme1580I G_kGCm'C
Cuts at: 1790 2044 3119 3460 5349 6505 1790
Size: 254 1075 341 1889 1156 2573
Fragments arranged by size:
2573 1889 1156 1075 341 254
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7288
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2019 2374 2983
Size: 416 53 83 399 24 602 355 609
Cuts at: 2983 3334 5019 5977 442
Size: 351 1685 958 1753
Fragments arranged by size:
1753 1685 958 609 602 416 399 355
351 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7288
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3866 3866
Size: 7288
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2183 3228 3874 4281 5948 1239
Size: 944 1045 646 407 1667 2579
Fragments arranged by size:
2579 1667 1045 944 646 407
Bpu10I CC'TnA_GC
Cuts at: 2115 3156 2115
Size: 1041 6247
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5413 6278 6576 5413
Size: 865 298 6125
Fragments arranged by size:
6125 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5951 882
Size: 617 4452 2219
Fragments arranged by size:
4452 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 5079 493
Size: 711 3875 2702
Fragments arranged by size:
3875 2702 711
BsaBI GATnn'nnATC
Cuts at: 2585 3417 7282 2585
Size: 832 3865 2591
Fragments arranged by size:
3865 2591 832
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 5095 5477 6112 275
Size: 53 83 186 936 3562 382 635 1451
Fragments arranged by size:
3562 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5721 6119 6393 6540 6951 5721
Size: 398 274 147 411 6058
Fragments arranged by size:
6058 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2181 2211 2213 2243 2559
Size: 30 165 30 778 30 2 30 316
Cuts at: 2559 2589 1178
Size: 30 5877
Fragments arranged by size:
5877 778 316 165 30 30 30 30
30 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2256 3858 1186
Size: 1070 1602 4616
Fragments arranged by size:
4616 1602 1070
BseYI C'CCAG_C
Cuts at: 3920 4085 6491 6987 3920
Size: 165 2406 496 4221
Fragments arranged by size:
4221 2406 496 165
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2785 1163
Size: 573 1049 5666
Fragments arranged by size:
5666 1049 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4738 5499 6527 1540
Size: 275 186 2737 761 1028 2301
Fragments arranged by size:
2737 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2996 3162 3460 3852 5349 5434 6505 2996
Size: 166 298 392 1497 85 1071 3779
Fragments arranged by size:
3779 1497 1071 392 298 166 85
BsmI GAATG_Cn'
Cuts at: 4799 4892 4799
Size: 93 7195
BsmBI CGTCTCn'nnnn_
Cuts at: 4113 4113
Size: 7288
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2020 2044 2075 2996 3119 3162 3460
Size: 399 230 24 31 921 123 43 298
Cuts at: 3460 3852 4029 4607 5349 5434 6505 1391
Size: 392 177 578 742 85 1071 2174
Fragments arranged by size:
2174 1071 921 742 578 399 392 298
230 177 123 85 43 31 24
BspEI T'CCGG_A
Cuts at: 6951 6951
Size: 7288
BspHI T'CATG_A
Cuts at: 2263 2670 5177 6907 2263
Size: 407 2507 1730 2644
Fragments arranged by size:
2644 2507 1730 407
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4643 844
Size: 3799 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 5175 1394
Size: 429 3352 3507
Fragments arranged by size:
3507 3352 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3801 4078 5783 5957 65
Size: 1183 2553 277 1705 174 1396
Fragments arranged by size:
2553 1705 1396 1183 277 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5932 6119 1700
Size: 110 4122 187 2869
Fragments arranged by size:
4122 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3731 96
Size: 3635 3653
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7288
BssSI C'ACGA_G
Cuts at: 3878 5348 6360 3878
Size: 1470 1012 4806
Fragments arranged by size:
4806 1470 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7288
BstBI TT'CG_AA
Cuts at: 2827 6092 2827
Size: 3265 4023
BstEII G'GTnAC_C
Cuts at: 1184 4309 1184
Size: 3125 4163
BstYI r'GATC_y
Cuts at: 1972 2467 4618 4985 5370 5387 6839 6925 6937
Size: 495 2151 367 385 17 1452 86 12
Cuts at: 6937 7283 1972
Size: 346 1977
Fragments arranged by size:
2151 1977 1452 495 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3775 3775
Size: 7288
BtgI C'CryG_G
Cuts at: 513 1065 2044 2149 3567 513
Size: 552 979 105 1418 4234
Fragments arranged by size:
4234 1418 979 552 105
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 4122 487
Size: 683 56 2896 3653
Fragments arranged by size:
3653 2896 683 56
BtsI GCAGTG_nn'
Cuts at: 2076 3128 4804 5598 5618 2076
Size: 1052 1676 794 20 3746
Fragments arranged by size:
3746 1676 1052 794 20
ClaI AT'CG_AT
Cuts at: 1554 3897 1554
Size: 2343 4945
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4146 4181 529
Size: 35 3582 35 3636
Fragments arranged by size:
3636 3582 35 35
DraI TTT'AAA
Cuts at: 3553 4592 3553
Size: 1039 6249
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6295 809
Size: 507 4979 1802
Fragments arranged by size:
4979 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2146 3798 4123
Size: 54 1425 50 275 18 316 1652 325
Cuts at: 4123 4469 4531 4735 5624 7253 8
Size: 346 62 204 889 1629 43
Fragments arranged by size:
1652 1629 1425 889 346 325 316 275
204 62 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4735 1537
Size: 275 2923 4090
Fragments arranged by size:
4090 2923 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2454 5218 1353
Size: 1101 2764 3423
Fragments arranged by size:
3423 2764 1101
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3940 5875 6249 6395 387
Size: 1064 138 2351 1935 374 146 1280
Fragments arranged by size:
2351 1935 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3160 3850 3160
Size: 690 6598
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2183 2310 2562 2759 3039 3228 3639
Size: 393 551 127 252 197 280 189 411
Cuts at: 3639 3874 3893 4256 4281 4433 5351 5948 6735
Size: 235 19 363 25 152 918 597 787
Cuts at: 6735 7003 1239
Size: 268 1524
Fragments arranged by size:
1524 918 787 597 551 411 393 363
280 268 252 235 197 189 152 127
25 19
EcoO109I rG'GnC_Cy
Cuts at: 2040 3115 3116 3137 2040
Size: 1075 1 21 6191
Fragments arranged by size:
6191 1075 21 1
EcoRI G'AATT_C
Cuts at: 4597 4975 4597
Size: 378 6910
EcoRV GAT'ATC
Cuts at: 2205 2205
Size: 7288
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3013 3013
Size: 7288
FspI TGC'GCA
Cuts at: 5794 5794
Size: 7288
HaeII r_GCGC'y
Cuts at: 1678 4435 6435 1678
Size: 2757 2000 2531
Fragments arranged by size:
2757 2531 2000
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2230 2262 2359 2391 3529
Size: 32 135 32 658 32 97 32 1138
Cuts at: 3529 3561 5929 5961 6003 6035 1373
Size: 32 2368 32 42 32 2626
Fragments arranged by size:
2626 2368 1138 658 135 97 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4632 4878 669
Size: 988 281 2694 246 3079
Fragments arranged by size:
3079 2694 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7288
HpaI GTT'AAC
Cuts at: 4878 4878
Size: 7288
KpnI G_GTAC'C
Cuts at: 4613 4613
Size: 7288
MfeI C'AATT_G
Cuts at: 2718 4887 2718
Size: 2169 5119
MluI A'CGCG_T
Cuts at: 7164 7164
Size: 7288
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3369 6402 6586 1305
Size: 2064 3033 184 2007
Fragments arranged by size:
3033 2064 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3800 4471 4533 10
Size: 54 1425 343 1968 671 62 2765
Fragments arranged by size:
2765 1968 1425 671 343 62 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3396 3572 3578 4386 5246 5605 5764
Size: 648 2230 176 6 808 860 359 159
Cuts at: 5764 518
Size: 2042
Fragments arranged by size:
2230 2042 860 808 648 359 176 159
6
MspA1I CmG'CkG
Cuts at: 1606 2046 2065 3920 3975 4129 4407 4704 5381
Size: 440 19 1855 55 154 278 297 677
Cuts at: 5381 6529 6774 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1855 1148 677 440 297 278 245
154 55 19
NcoI C'CATG_G
Cuts at: 513 1065 3567 513
Size: 552 2502 4234
Fragments arranged by size:
4234 2502 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7288
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7288
NotI GC'GGCC_GC
Cuts at: 4735 4735
Size: 7288
NspI r_CATG'y
Cuts at: 1132 1229 3577 4084 4646 6191 1132
Size: 97 2348 507 562 1545 2229
Fragments arranged by size:
2348 2229 1545 562 507 97
PasI CC'CwG_GG
Cuts at: 1348 3119 1348
Size: 1771 5517
PciI A'CATG_T
Cuts at: 6187 6187
Size: 7288
PflMI CCAn_nnn'nTGG
Cuts at: 1108 4286 4516 1108
Size: 3178 230 3880
Fragments arranged by size:
3880 3178 230
PfoI T'CCnGG_A
Cuts at: 4285 4285
Size: 7288
PmeI GTTT'AAAC
Cuts at: 4592 4592
Size: 7288
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5357 5389 6870 6902 5357
Size: 32 1481 32 5743
Fragments arranged by size:
5743 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7288
PsiI TTA'TAA
Cuts at: 2703 4858 2703
Size: 2155 5133
PspOMI G'GGCC_C
Cuts at: 2040 3115 2040
Size: 1075 6213
PstI C_TGCA'G
Cuts at: 830 4640 830
Size: 3810 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7288
PvuII CAG'CTG
Cuts at: 2065 3920 4129 2065
Size: 1855 209 5224
Fragments arranged by size:
5224 1855 209
SacI G_AGCT'C
Cuts at: 3162 3852 3162
Size: 690 6598
SacII CC_GC'GG
Cuts at: 2047 2047
Size: 7288
SalI G'TCGA_C
Cuts at: 1936 4630 1936
Size: 2694 4594
SapI GCTCTTCn'nnn_
Cuts at: 2454 2454
Size: 7288
SbfI CC_TGCA'GG
Cuts at: 4640 4640
Size: 7288
ScaI AGT'ACT
Cuts at: 5536 7248 5536
Size: 1712 5576
SexAI A'CCwGG_T
Cuts at: 2092 2092
Size: 7288
SfcI C'TryA_G
Cuts at: 826 1045 2525 3040 3821 4636 5771 6452 6643
Size: 219 1480 515 781 815 1135 681 191
Cuts at: 6643 826
Size: 1471
Fragments arranged by size:
1480 1471 1135 815 781 681 515 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7288
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7288
SmaI CCC'GGG
Cuts at: 1872 4615 1872
Size: 2743 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3628 5392 6293 6555 820
Size: 197 926 1685 1764 901 262 1553
Fragments arranged by size:
1764 1685 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 5079 493
Size: 4586 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7288
SphI G_CATG'C
Cuts at: 1132 3577 4646 1132
Size: 2445 1069 3774
Fragments arranged by size:
3774 2445 1069
SspI AAT'ATT
Cuts at: 5 52 2644 5212 5
Size: 47 2592 2568 2081
Fragments arranged by size:
2592 2568 2081 47
StuI AGG'CCT
Cuts at: 1517 2113 1517
Size: 596 6692
StyI C'CwwG_G
Cuts at: 513 1065 2108 3394 3567 4727 513
Size: 552 1043 1286 173 1160 3074
Fragments arranged by size:
3074 1286 1160 1043 552 173
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3677 5315 5332 5485 5670 6115 325
Size: 1255 2097 1638 17 153 185 445 1498
Fragments arranged by size:
2097 1638 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3731 4077 5534
Size: 274 80 33 51 1443 1754 346 1457
Cuts at: 5534 7246 96
Size: 1712 138
Fragments arranged by size:
1754 1712 1457 1443 346 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2181 2213 2559 2591 7248 7280 2181
Size: 32 346 32 4657 32 2189
Fragments arranged by size:
4657 2189 346 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7288
XbaI T'CTAG_A
Cuts at: 4624 4624
Size: 7288
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2207 3486 2207
Size: 1279 6009
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7288
XmaI C'CCGG_G
Cuts at: 1870 4613 1870
Size: 2743 4545
XmnI GAAnn'nnTTC
Cuts at: 3387 5417 3387
Size: 2030 5258
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5096 276
Size: 53 83 186 936 3562 2468
Fragments arranged by size:
3562 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI BaeI BbeI BfrBI BsiWI BstXI BstZ17I
DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII
SanDI SfiI SfoI SrfI SwaI