Restriction map for
pFN21AE9615
(Circular) (Six-base) MAPSORT of: pFN21AE9615.seq Check: 3413 from: 1 to: 5749
pFN21AE9615 5749 bp
With 182 enzymes: SgfI *
July 6, 2012 17:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2926 2926
Size: 5749
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3559 278
Size: 53 83 186 936 2023 2468
Fragments arranged by size:
2468 2023 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3092 1937
Size: 1155 4594
Acc65I G'GTAC_C
Cuts at: 3070 3070
Size: 5749
AclI AA'CG_TT
Cuts at: 1563 3876 4249 1563
Size: 2313 373 3063
Fragments arranged by size:
3063 2313 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2111 2714 3812 5196 5464 1632
Size: 479 603 1098 1384 268 1917
Fragments arranged by size:
1917 1384 1098 603 479 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5552
AflIII A'CryG_T
Cuts at: 1203 4648 5625 1203
Size: 3445 977 1327
Fragments arranged by size:
3445 1327 977
AgeI A'CCGG_T
Cuts at: 4580 4580
Size: 5749
AhdI GACnn_n'nnGTC
Cuts at: 4478 4478
Size: 5749
AleI CACnn'nnGTG
Cuts at: 1166 2984 1166
Size: 1818 3931
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5749
AlwNI CAG_nnn'CTG
Cuts at: 2674 3492 5064 2674
Size: 818 1572 3359
Fragments arranged by size:
3359 1572 818
ApaI G_GGCC'C
Cuts at: 2681 2681
Size: 5749
ApaLI G'TGCA_C
Cuts at: 2249 3806 4962 2249
Size: 1557 1156 3036
Fragments arranged by size:
3036 1557 1156
ApoI r'AATT_y
Cuts at: 1493 3058 3284 3436 5545 1493
Size: 1565 226 152 2109 1697
Fragments arranged by size:
2109 1697 1565 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4303 160
Size: 872 3271 1606
Fragments arranged by size:
3271 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5749
AvaI C'yCGr_G
Cuts at: 1870 1943 2134 2214 3074 1870
Size: 73 191 80 860 4545
Fragments arranged by size:
4545 860 191 80 73
BamHI G'GATC_C
Cuts at: 2056 3079 3446 2056
Size: 1023 367 4359
Fragments arranged by size:
4359 1023 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2098 3070 4525 618
Size: 325 217 627 311 972 1455 1842
Fragments arranged by size:
1842 1455 972 627 325 311 217
BanII G_rGCy'C
Cuts at: 1391 2401 2681 2871 3068 1391
Size: 1010 280 190 197 4072
Fragments arranged by size:
4072 1010 280 197 190
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2119 2721 928
Size: 971 220 602 3956
Fragments arranged by size:
3956 971 602 220
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3940 3974 1903
Size: 34 2003 34 3678
Fragments arranged by size:
3678 2003 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3641 4851 3641
Size: 1210 4539
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5749
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2243 4360 136
Size: 107 122 71 631 605 571 2117 1525
Fragments arranged by size:
2117 1525 631 605 571 122 107 71
BglII A'GATC_T
Cuts at: 2880 5744 2880
Size: 2864 2885
BlpI GC'TnA_GC
Cuts at: 3166 3166
Size: 5749
Bme1580I G_kGCm'C
Cuts at: 1790 2253 2467 2681 3810 4966 1790
Size: 463 214 214 1129 1156 2573
Fragments arranged by size:
2573 1156 1129 463 214 214
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5749
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2482 2499 3480
Size: 416 53 83 399 24 1065 17 981
Cuts at: 3480 4438 442
Size: 958 1753
Fragments arranged by size:
1753 1065 981 958 416 399 83 53
24 17
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5749
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2049 2049
Size: 5749
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2794 4409 1239
Size: 1555 1615 2579
Fragments arranged by size:
2579 1615 1555
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2330 3874 4739 5037 2330
Size: 1544 865 298 3042
Fragments arranged by size:
3042 1544 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2770 4412 882
Size: 617 1271 1642 2219
Fragments arranged by size:
2219 1642 1271 617
BsaAI yAC'GTr
Cuts at: 493 1204 3540 493
Size: 711 2336 2702
Fragments arranged by size:
2702 2336 711
BsaBI GATnn'nnATC
Cuts at: 2885 5743 2885
Size: 2858 2891
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2124 3556 3938 4573
Size: 53 83 186 936 591 1432 382 635
Cuts at: 4573 275
Size: 1451
Fragments arranged by size:
1451 1432 936 635 591 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4182 4580 4854 5001 5412 4182
Size: 398 274 147 411 4519
Fragments arranged by size:
4519 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2372 2402 1178
Size: 30 165 30 969 30 4525
Fragments arranged by size:
4525 969 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2663 1186
Size: 1477 4272
BseYI C'CCAG_C
Cuts at: 2266 2401 4952 5448 2266
Size: 135 2551 496 2567
Fragments arranged by size:
2567 2551 496 135
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2072 2141 2171 2303 2318 1163
Size: 573 336 69 30 132 15 4594
Fragments arranged by size:
4594 573 336 132 69 30 15
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3199 3960 4988 1540
Size: 275 186 1198 761 1028 2301
Fragments arranged by size:
2301 1198 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2253 3810 3895 4966 2253
Size: 1557 85 1071 3036
Fragments arranged by size:
3036 1557 1071 85
BsmI GAATG_Cn'
Cuts at: 3260 3353 3260
Size: 93 5656
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2253 2401 2467 2681 2871 3068 3810
Size: 399 463 148 66 214 190 197 742
Cuts at: 3810 3895 4966 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 463 399 214 197 190
148 85 66
BspEI T'CCGG_A
Cuts at: 5412 5412
Size: 5749
BspHI T'CATG_A
Cuts at: 2322 3638 5368 2322
Size: 1316 1730 2703
Fragments arranged by size:
2703 1730 1316
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2926 3104 844
Size: 2082 178 3489
Fragments arranged by size:
3489 2082 178
BsrBI CCG'CTC
Cuts at: 1394 1823 2275 2461 2920 3636 1394
Size: 429 452 186 459 716 3507
Fragments arranged by size:
3507 716 459 452 429 186
BsrDI GCAATG_nn'
Cuts at: 65 1248 2311 4244 4418 65
Size: 1183 1063 1933 174 1396
Fragments arranged by size:
1933 1396 1183 1063 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2095 4393 4580 1700
Size: 110 285 2298 187 2869
Fragments arranged by size:
2869 2298 285 187 110
BsrGI T'GTAC_A
Cuts at: 96 2007 96
Size: 1911 3838
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5749
BssSI C'ACGA_G
Cuts at: 3809 4821 3809
Size: 1012 4737
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5749
BstBI TT'CG_AA
Cuts at: 4553 4553
Size: 5749
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5749
BstXI CCAn_nnnn'nTGG
Cuts at: 2360 2557 2360
Size: 197 5552
BstYI r'GATC_y
Cuts at: 1972 2056 2880 3079 3446 3831 3848 5300 5386
Size: 84 824 199 367 385 17 1452 86
Cuts at: 5386 5398 5744 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 824 385 367 346 199 86
84 17 12
Bsu36I CC'TnA_GG
Cuts at: 2709 3000 2709
Size: 291 5458
BtgI C'CryG_G
Cuts at: 513 1065 2336 513
Size: 552 1271 3926
Fragments arranged by size:
3926 1271 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5010
Fragments arranged by size:
5010 683 56
BtsI GCAGTG_nn'
Cuts at: 3265 4059 4079 3265
Size: 794 20 4935
Fragments arranged by size:
4935 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5749
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5714
DraI TTT'AAA
Cuts at: 3053 3053
Size: 5749
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4756 809
Size: 507 3440 1802
Fragments arranged by size:
3440 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2589 2940 3196
Size: 54 1425 50 275 18 759 351 256
Cuts at: 3196 4085 5714 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 759 351 275 256 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3196 1537
Size: 275 1384 4090
Fragments arranged by size:
4090 1384 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2166 2324 3679 1353
Size: 813 158 1355 3423
Fragments arranged by size:
3423 1355 813 158
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4336 4710 4856 387
Size: 1064 138 2747 374 146 1280
Fragments arranged by size:
2747 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2111 2714 2794 3812 4409 5196 5464
Size: 393 479 603 80 1018 597 787 268
Cuts at: 5464 1239
Size: 1524
Fragments arranged by size:
1524 1018 787 603 597 479 393 268
80
EcoNI CCTnn'n_nnAGG
Cuts at: 2795 2795
Size: 5749
EcoO109I rG'GnC_Cy
Cuts at: 2229 2341 2578 2671 2678 2229
Size: 112 237 93 7 5300
Fragments arranged by size:
5300 237 112 93 7
EcoRI G'AATT_C
Cuts at: 3058 3436 3058
Size: 378 5371
EcoRV GAT'ATC
Cuts at: 2838 2838
Size: 5749
FspI TGC'GCA
Cuts at: 4255 4255
Size: 5749
HaeII r_GCGC'y
Cuts at: 1678 4896 1678
Size: 3218 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2983 3015 4390 4422 4464
Size: 32 135 32 1411 32 1375 32 42
Cuts at: 4464 4496 1373
Size: 32 2626
Fragments arranged by size:
2626 1411 1375 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3093 3339 669
Size: 988 281 1155 246 3079
Fragments arranged by size:
3079 1155 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5749
HpaI GTT'AAC
Cuts at: 3339 3339
Size: 5749
KpnI G_GTAC'C
Cuts at: 3074 3074
Size: 5749
MfeI C'AATT_G
Cuts at: 3348 3348
Size: 5749
MluI A'CGCG_T
Cuts at: 5625 5625
Size: 5749
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2574 4863 5047 1305
Size: 1269 2289 184 2007
Fragments arranged by size:
2289 2007 1269 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2591 10
Size: 54 1425 343 759 3168
Fragments arranged by size:
3168 1425 759 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2984 3707 4066 4225 518
Size: 648 1818 723 359 159 2042
Fragments arranged by size:
2042 1818 723 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2405 2726 3165 3842 4990 5235 1606
Size: 799 321 439 677 1148 245 2120
Fragments arranged by size:
2120 1148 799 677 439 321 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5197
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5749
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5749
NotI GC'GGCC_GC
Cuts at: 3196 3196
Size: 5749
NspI r_CATG'y
Cuts at: 1132 1229 2072 2965 3107 4652 1132
Size: 97 843 893 142 1545 2229
Fragments arranged by size:
2229 1545 893 843 142 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5749
PciI A'CATG_T
Cuts at: 4648 4648
Size: 5749
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2674 1108
Size: 1566 4183
PfoI T'CCnGG_A
Cuts at: 2505 2556 2595 2505
Size: 51 39 5659
Fragments arranged by size:
5659 51 39
PmeI GTTT'AAAC
Cuts at: 3053 3053
Size: 5749
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3818 3850 5331 5363 3818
Size: 32 1481 32 4204
Fragments arranged by size:
4204 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2578 2671 2578
Size: 93 5656
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5749
PsiI TTA'TAA
Cuts at: 3319 3319
Size: 5749
PspOMI G'GGCC_C
Cuts at: 2677 2677
Size: 5749
PstI C_TGCA'G
Cuts at: 830 2937 3101 830
Size: 2107 164 3478
Fragments arranged by size:
3478 2107 164
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5749
PvuII CAG'CTG
Cuts at: 2405 2726 2405
Size: 321 5428
SalI G'TCGA_C
Cuts at: 1936 3091 1936
Size: 1155 4594
SbfI CC_TGCA'GG
Cuts at: 2937 3101 2937
Size: 164 5585
ScaI AGT'ACT
Cuts at: 3997 5709 3997
Size: 1712 4037
SexAI A'CCwGG_T
Cuts at: 2424 2424
Size: 5749
SfcI C'TryA_G
Cuts at: 826 1045 2933 3097 4232 4913 5104 826
Size: 219 1888 164 1135 681 191 1471
Fragments arranged by size:
1888 1471 1135 681 219 191 164
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5749
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5749
SmaI CCC'GGG
Cuts at: 1872 2216 3076 1872
Size: 344 860 4545
Fragments arranged by size:
4545 860 344
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2345 3853 4754 5016 820
Size: 197 926 402 1508 901 262 1553
Fragments arranged by size:
1553 1508 926 901 402 262 197
SnaBI TAC'GTA
Cuts at: 493 3540 493
Size: 3047 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5749
SphI G_CATG'C
Cuts at: 1132 2965 3107 1132
Size: 1833 142 3774
Fragments arranged by size:
3774 1833 142
SspI AAT'ATT
Cuts at: 5 52 3673 5
Size: 47 3621 2081
Fragments arranged by size:
3621 2081 47
StuI AGG'CCT
Cuts at: 1517 2064 1517
Size: 547 5202
StyI C'CwwG_G
Cuts at: 513 1065 2796 3188 513
Size: 552 1731 392 3074
Fragments arranged by size:
3074 1731 552 392
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3776 3793 3946 4131 4576 325
Size: 1255 2196 17 153 185 445 1498
Fragments arranged by size:
2196 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2007 3995 5707
Size: 274 80 33 51 1443 30 1988 1712
Cuts at: 5707 96
Size: 138
Fragments arranged by size:
1988 1712 1443 274 138 80 51 33
30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2002 2034 2372 2404 5709 5741 2002
Size: 32 338 32 3305 32 2010
Fragments arranged by size:
3305 2010 338 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5749
XbaI T'CTAG_A
Cuts at: 3085 3085
Size: 5749
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5749
XmaI C'CCGG_G
Cuts at: 1870 2214 3074 1870
Size: 344 860 4545
Fragments arranged by size:
4545 860 344
XmnI GAAnn'nnTTC
Cuts at: 2525 3878 2525
Size: 1353 4396
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3557 276
Size: 53 83 186 936 2023 2468
Fragments arranged by size:
2468 2023 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SacI SacII SanDI SapI
SfiI SfoI SrfI SwaI XcmI