Restriction map for
pFN21AE9612
(Circular) (Six-base) MAPSORT of: pFN21AE9612.seq Check: 595 from: 1 to: 5641
pFN21AE9612 5641 bp
With 182 enzymes: SgfI *
March 10, 2017 10:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3451 278
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2173 2984 1937
Size: 236 811 4594
Fragments arranged by size:
4594 811 236
Acc65I G'GTAC_C
Cuts at: 2962 2962
Size: 5641
AclI AA'CG_TT
Cuts at: 1563 3768 4141 1563
Size: 2205 373 3063
Fragments arranged by size:
3063 2205 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2700 3704 5088 5356 1632
Size: 1068 1004 1384 268 1917
Fragments arranged by size:
1917 1384 1068 1004 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5444
AflIII A'CryG_T
Cuts at: 1203 2052 2364 4540 5517 1203
Size: 849 312 2176 977 1327
Fragments arranged by size:
2176 1327 977 849 312
AgeI A'CCGG_T
Cuts at: 4472 4472
Size: 5641
AhdI GACnn_n'nnGTC
Cuts at: 4370 4370
Size: 5641
AleI CACnn'nnGTG
Cuts at: 1166 2273 1166
Size: 1107 4534
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2402 1184
Size: 1218 4423
AlwNI CAG_nnn'CTG
Cuts at: 2182 3384 4956 2182
Size: 1202 1572 2867
Fragments arranged by size:
2867 1572 1202
ApaLI G'TGCA_C
Cuts at: 3698 4854 3698
Size: 1156 4485
ApoI r'AATT_y
Cuts at: 1493 2950 3176 3328 5437 1493
Size: 1457 226 152 2109 1697
Fragments arranged by size:
2109 1697 1457 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4195 160
Size: 872 3163 1606
Fragments arranged by size:
3163 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
AvaI C'yCGr_G
Cuts at: 1870 1943 2565 2966 1870
Size: 73 622 401 4545
Fragments arranged by size:
4545 622 401 73
BamHI G'GATC_C
Cuts at: 2278 2971 3338 2278
Size: 693 367 4581
Fragments arranged by size:
4581 693 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2039 2962 4417 618
Size: 325 217 627 252 923 1455 1842
Fragments arranged by size:
1842 1455 923 627 325 252 217
BanII G_rGCy'C
Cuts at: 1391 2960 1391
Size: 1569 4072
BbeI G_GCGC'C
Cuts at: 2043 2043
Size: 5641
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2246 2495 2822 928
Size: 971 347 249 327 3747
Fragments arranged by size:
3747 971 347 327 249
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3832 3866 1903
Size: 34 1895 34 3678
Fragments arranged by size:
3678 1895 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3533 4743 3533
Size: 1210 4431
BclI T'GATC_A
Cuts at: 1548 2344 1548
Size: 796 4845
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4252 136
Size: 107 122 71 631 605 2580 1525
Fragments arranged by size:
2580 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5636 5636
Size: 5641
BlpI GC'TnA_GC
Cuts at: 3058 3058
Size: 5641
Bme1580I G_kGCm'C
Cuts at: 1790 3702 4858 1790
Size: 1912 1156 2573
Fragments arranged by size:
2573 1912 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5641
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3372 4330 442
Size: 416 53 83 399 24 1955 958 1753
Fragments arranged by size:
1955 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5641
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2273 2273
Size: 5641
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4301 1239
Size: 3062 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2426 3766 4631 4929 2426
Size: 1340 865 298 3138
Fragments arranged by size:
3138 1340 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4304 882
Size: 617 2805 2219
Fragments arranged by size:
2805 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2053 3432 493
Size: 711 849 1379 2702
Fragments arranged by size:
2702 1379 849 711
BsaBI GATnn'nnATC
Cuts at: 5635 5635
Size: 5641
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2040 3448 3830 4465
Size: 53 83 186 936 507 1408 382 635
Cuts at: 4465 275
Size: 1451
Fragments arranged by size:
1451 1408 936 635 507 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4074 4472 4746 4893 5304 4074
Size: 398 274 147 411 4411
Fragments arranged by size:
4411 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2315 2345 1178
Size: 30 165 30 912 30 4474
Fragments arranged by size:
4474 912 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5641
BseYI C'CCAG_C
Cuts at: 4844 5340 4844
Size: 496 5145
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5068
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3091 3852 4880 1540
Size: 275 186 1090 761 1028 2301
Fragments arranged by size:
2301 1090 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3702 3787 4858 3702
Size: 85 1071 4485
Fragments arranged by size:
4485 1071 85
BsmI GAATG_Cn'
Cuts at: 3152 3245 3152
Size: 93 5548
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2960 3702 3787 4858 1391
Size: 399 1170 742 85 1071 2174
Fragments arranged by size:
2174 1170 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5304 5304
Size: 5641
BspHI T'CATG_A
Cuts at: 3530 5260 3530
Size: 1730 3911
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2996 844
Size: 2152 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3528 1394
Size: 429 1705 3507
Fragments arranged by size:
3507 1705 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2723 4136 4310 65
Size: 1183 1475 1413 174 1396
Fragments arranged by size:
1475 1413 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4285 4472 1700
Size: 110 2475 187 2869
Fragments arranged by size:
2869 2475 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5641
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5641
BssSI C'ACGA_G
Cuts at: 3701 4713 3701
Size: 1012 4629
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5641
BstBI TT'CG_AA
Cuts at: 4445 4445
Size: 5641
BstEII G'GTnAC_C
Cuts at: 1184 2594 1184
Size: 1410 4231
BstXI CCAn_nnnn'nTGG
Cuts at: 2275 2275
Size: 5641
BstYI r'GATC_y
Cuts at: 1972 2278 2971 3338 3723 3740 5192 5278 5290
Size: 306 693 367 385 17 1452 86 12
Cuts at: 5290 5636 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 693 385 367 346 306 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2641 513
Size: 552 939 637 3513
Fragments arranged by size:
3513 939 637 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4902
Fragments arranged by size:
4902 683 56
BtsI GCAGTG_nn'
Cuts at: 3157 3951 3971 3157
Size: 794 20 4827
Fragments arranged by size:
4827 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5641
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5606
DraI TTT'AAA
Cuts at: 2076 2945 2076
Size: 869 4772
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4648 809
Size: 507 3332 1802
Fragments arranged by size:
3332 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 3088 3977
Size: 54 1425 50 275 18 177 1081 889
Cuts at: 3977 5606 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1081 889 275 177 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3088 1537
Size: 275 1276 4090
Fragments arranged by size:
4090 1276 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2613 3571 1353
Size: 1260 958 3423
Fragments arranged by size:
3423 1260 958
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4228 4602 4748 387
Size: 1064 138 2639 374 146 1280
Fragments arranged by size:
2639 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2700 3704 4301 5088 5356 1239
Size: 393 1068 1004 597 787 268 1524
Fragments arranged by size:
1524 1068 1004 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2309 2309
Size: 5641
EcoRI G'AATT_C
Cuts at: 2950 3328 2950
Size: 378 5263
EcoRV GAT'ATC
Cuts at: 2652 2652
Size: 5641
FspI TGC'GCA
Cuts at: 4147 4147
Size: 5641
HaeII r_GCGC'y
Cuts at: 1678 2043 4788 1678
Size: 365 2745 2531
Fragments arranged by size:
2745 2531 365
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2672 2704 2833 2865 4282
Size: 32 135 32 1100 32 129 32 1417
Cuts at: 4282 4314 4356 4388 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1417 1100 135 129 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2128 2577 2872 2985 3231 669
Size: 988 281 190 449 295 113 246 3079
Fragments arranged by size:
3079 988 449 295 281 246 190 113
HindIII A'AGCT_T
Cuts at: 748 2601 2702 748
Size: 1853 101 3687
Fragments arranged by size:
3687 1853 101
HpaI GTT'AAC
Cuts at: 2128 3231 2128
Size: 1103 4538
KasI G'GCGC_C
Cuts at: 2039 2039
Size: 5641
KpnI G_GTAC'C
Cuts at: 2966 2966
Size: 5641
MfeI C'AATT_G
Cuts at: 3240 3240
Size: 5641
MluI A'CGCG_T
Cuts at: 5517 5517
Size: 5641
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4755 4939 1305
Size: 3450 184 2007
Fragments arranged by size:
3450 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3819
Fragments arranged by size:
3819 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2273 2497 3599 3958 4117 518
Size: 648 1107 224 1102 359 159 2042
Fragments arranged by size:
2042 1107 1102 648 359 224 159
MspA1I CmG'CkG
Cuts at: 1606 2012 2182 3057 3734 4882 5127 1606
Size: 406 170 875 677 1148 245 2120
Fragments arranged by size:
2120 1148 875 677 406 245 170
NarI GG'CG_CC
Cuts at: 2040 2040
Size: 5641
NcoI C'CATG_G
Cuts at: 513 1065 2004 2641 513
Size: 552 939 637 3513
Fragments arranged by size:
3513 939 637 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5641
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5641
NotI GC'GGCC_GC
Cuts at: 3088 3088
Size: 5641
NspI r_CATG'y
Cuts at: 1132 1229 2999 4544 1132
Size: 97 1770 1545 2229
Fragments arranged by size:
2229 1770 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5641
PciI A'CATG_T
Cuts at: 4540 4540
Size: 5641
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5641
PfoI T'CCnGG_A
Cuts at: 2398 2398
Size: 5641
PmeI GTTT'AAAC
Cuts at: 2945 2945
Size: 5641
PmlI CAC'GTG
Cuts at: 2053 2053
Size: 5641
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3710 3742 5223 5255 3710
Size: 32 1481 32 4096
Fragments arranged by size:
4096 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5641
PsiI TTA'TAA
Cuts at: 2228 3211 2228
Size: 983 4658
PstI C_TGCA'G
Cuts at: 830 2848 2993 830
Size: 2018 145 3478
Fragments arranged by size:
3478 2018 145
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5641
PvuII CAG'CTG
Cuts at: 2182 2182
Size: 5641
SalI G'TCGA_C
Cuts at: 1936 2983 1936
Size: 1047 4594
SbfI CC_TGCA'GG
Cuts at: 2993 2993
Size: 5641
ScaI AGT'ACT
Cuts at: 3889 5601 3889
Size: 1712 3929
SfcI C'TryA_G
Cuts at: 826 1045 2174 2769 2844 2989 4124 4805 4996
Size: 219 1129 595 75 145 1135 681 191
Cuts at: 4996 826
Size: 1471
Fragments arranged by size:
1471 1135 1129 681 595 219 191 145
75
SfoI GGC'GCC
Cuts at: 2041 2041
Size: 5641
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5641
SmaI CCC'GGG
Cuts at: 1872 2567 2968 1872
Size: 695 401 4545
Fragments arranged by size:
4545 695 401
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2405 3745 4646 4908 820
Size: 197 926 462 1340 901 262 1553
Fragments arranged by size:
1553 1340 926 901 462 262 197
SnaBI TAC'GTA
Cuts at: 493 3432 493
Size: 2939 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5641
SphI G_CATG'C
Cuts at: 1132 2999 1132
Size: 1867 3774
SspI AAT'ATT
Cuts at: 5 52 2072 2468 3565 5
Size: 47 2020 396 1097 2081
Fragments arranged by size:
2081 2020 1097 396 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5641
StyI C'CwwG_G
Cuts at: 513 1065 2004 2641 3080 513
Size: 552 939 637 439 3074
Fragments arranged by size:
3074 939 637 552 439
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3668 3685 3838 4023 4468 325
Size: 1255 2088 17 153 185 445 1498
Fragments arranged by size:
2088 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2475 3887 5599
Size: 274 80 33 51 1443 498 1412 1712
Cuts at: 5599 96
Size: 138
Fragments arranged by size:
1712 1443 1412 498 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5601 5633 5601
Size: 32 5609
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5641
XbaI T'CTAG_A
Cuts at: 2977 2977
Size: 5641
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5641
XmaI C'CCGG_G
Cuts at: 1870 2565 2966 1870
Size: 695 401 4545
Fragments arranged by size:
4545 695 401
XmnI GAAnn'nnTTC
Cuts at: 3770 3770
Size: 5641
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3449 276
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI EcoRV FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI
BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoO109I FalI FseI FspAI NaeI NgoMIV NruI NsiI
PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SrfI SwaI XcmI