Restriction map for
pFN21AE9610
(Circular) (Six-base) MAPSORT of: pFN21AE9610.seq Check: 8442 from: 1 to: 5602
pFN21AE9610 5602 bp
With 182 enzymes: SgfI *
March 10, 2017 10:57 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3412 278
Size: 53 83 186 936 1876 2468
Fragments arranged by size:
2468 1876 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2142 2945 1937
Size: 205 803 4594
Fragments arranged by size:
4594 803 205
Acc65I G'GTAC_C
Cuts at: 2923 2923
Size: 5602
AclI AA'CG_TT
Cuts at: 1563 3729 4102 1563
Size: 2166 373 3063
Fragments arranged by size:
3063 2166 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3665 5049 5317 1632
Size: 2033 1384 268 1917
Fragments arranged by size:
2033 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5405
AflIII A'CryG_T
Cuts at: 1203 4501 5478 1203
Size: 3298 977 1327
Fragments arranged by size:
3298 1327 977
AgeI A'CCGG_T
Cuts at: 4433 4433
Size: 5602
AhdI GACnn_n'nnGTC
Cuts at: 4331 4331
Size: 5602
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5602
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5602
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2669 2701 2669
Size: 32 5570
AlwNI CAG_nnn'CTG
Cuts at: 2369 2544 3345 4917 2369
Size: 175 801 1572 3054
Fragments arranged by size:
3054 1572 801 175
ApaI G_GGCC'C
Cuts at: 2123 2135 2123
Size: 12 5590
ApaLI G'TGCA_C
Cuts at: 3659 4815 3659
Size: 1156 4446
ApoI r'AATT_y
Cuts at: 1493 2911 3137 3289 5398 1493
Size: 1418 226 152 2109 1697
Fragments arranged by size:
2109 1697 1418 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4156 160
Size: 872 3124 1606
Fragments arranged by size:
3124 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5602
AvaI C'yCGr_G
Cuts at: 1870 1943 2477 2559 2927 1870
Size: 73 534 82 368 4545
Fragments arranged by size:
4545 534 368 82 73
BamHI G'GATC_C
Cuts at: 2932 3299 2932
Size: 367 5235
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2923 4378 618
Size: 325 217 627 1136 1455 1842
Fragments arranged by size:
1842 1455 1136 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2123 2135 2921 1391
Size: 732 12 786 4072
Fragments arranged by size:
4072 786 732 12
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4631
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3793 3827 1903
Size: 34 1856 34 3678
Fragments arranged by size:
3678 1856 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2605 3494 4704 2605
Size: 889 1210 3503
Fragments arranged by size:
3503 1210 889
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5602
BfrBI ATG'CAT
Cuts at: 2387 2387
Size: 5602
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2555 4213 136
Size: 107 122 71 631 605 883 1658 1525
Fragments arranged by size:
1658 1525 883 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2179 5597 2179
Size: 3418 2184
BlpI GC'TnA_GC
Cuts at: 3019 3019
Size: 5602
Bme1580I G_kGCm'C
Cuts at: 1790 2067 2123 2135 3663 4819 1790
Size: 277 56 12 1528 1156 2573
Fragments arranged by size:
2573 1528 1156 277 56 12
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5602
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2379 2692 3333
Size: 416 53 83 399 24 962 313 641
Cuts at: 3333 4291 442
Size: 958 1753
Fragments arranged by size:
1753 962 958 641 416 399 313 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5602
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2042 2204 2759 2903 4262 1239
Size: 803 162 555 144 1359 2579
Fragments arranged by size:
2579 1359 803 555 162 144
Bpu10I CC'TnA_GC
Cuts at: 2626 2626
Size: 5602
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3727 4592 4890 3727
Size: 865 298 4439
Fragments arranged by size:
4439 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4265 882
Size: 617 2766 2219
Fragments arranged by size:
2766 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2674 3393 493
Size: 711 1470 719 2702
Fragments arranged by size:
2702 1470 719 711
BsaBI GATnn'nnATC
Cuts at: 5596 5596
Size: 5602
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2100 3409 3791 4426
Size: 53 83 186 936 567 1309 382 635
Cuts at: 4426 275
Size: 1451
Fragments arranged by size:
1451 1309 936 635 567 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2706 4035 4433 4707 4854 5265 2706
Size: 1329 398 274 147 411 3043
Fragments arranged by size:
3043 1329 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2330 2360 2669 2699 1178
Size: 30 165 30 927 30 309 30 4081
Fragments arranged by size:
4081 927 309 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5602
BseYI C'CCAG_C
Cuts at: 2128 2274 4805 5301 2128
Size: 146 2531 496 2429
Fragments arranged by size:
2531 2429 496 146
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5029
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3052 3813 4841 1540
Size: 275 186 1051 761 1028 2301
Fragments arranged by size:
2301 1051 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2673 3663 3748 4819 2673
Size: 990 85 1071 3456
Fragments arranged by size:
3456 1071 990 85
BsmI GAATG_Cn'
Cuts at: 3113 3206 3113
Size: 93 5509
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2067 2123 2135 2673 2921 3663 3748
Size: 399 277 56 12 538 248 742 85
Cuts at: 3748 4819 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 538 399 277 248 85
56 12
BspEI T'CCGG_A
Cuts at: 5265 5265
Size: 5602
BspHI T'CATG_A
Cuts at: 3491 5221 3491
Size: 1730 3872
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2957 844
Size: 2113 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2230 3489 1394
Size: 429 407 1259 3507
Fragments arranged by size:
3507 1259 429 407
BsrDI GCAATG_nn'
Cuts at: 65 1248 4097 4271 65
Size: 1183 2849 174 1396
Fragments arranged by size:
2849 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4246 4433 1700
Size: 110 2436 187 2869
Fragments arranged by size:
2869 2436 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5602
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5602
BssSI C'ACGA_G
Cuts at: 3662 4674 3662
Size: 1012 4590
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5602
BstBI TT'CG_AA
Cuts at: 4406 4406
Size: 5602
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5602
BstYI r'GATC_y
Cuts at: 1972 2008 2179 2932 3299 3684 3701 5153 5239
Size: 36 171 753 367 385 17 1452 86
Cuts at: 5239 5251 5597 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 753 385 367 346 171 86
36 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2067 2136 513
Size: 552 939 63 69 3979
Fragments arranged by size:
3979 939 552 69 63
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4863
Fragments arranged by size:
4863 683 56
BtsI GCAGTG_nn'
Cuts at: 3118 3912 3932 3118
Size: 794 20 4788
Fragments arranged by size:
4788 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5602
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5567
DraI TTT'AAA
Cuts at: 2906 2906
Size: 5602
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4609 809
Size: 507 3293 1802
Fragments arranged by size:
3293 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3049 3938 5567
Size: 54 1425 50 275 18 1219 889 1629
Cuts at: 5567 8
Size: 43
Fragments arranged by size:
1629 1425 1219 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3049 1537
Size: 275 1237 4090
Fragments arranged by size:
4090 1237 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3532 1353
Size: 2179 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2765 4189 4563 4709 387
Size: 1064 138 1176 1424 374 146 1280
Fragments arranged by size:
1424 1280 1176 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2042 2204 2759 2903 3665 4262 5049
Size: 393 410 162 555 144 762 597 787
Cuts at: 5049 5317 1239
Size: 268 1524
Fragments arranged by size:
1524 787 762 597 555 410 393 268
162 144
EcoO109I rG'GnC_Cy
Cuts at: 2119 2120 2132 2832 2119
Size: 1 12 700 4889
Fragments arranged by size:
4889 700 12 1
EcoRI G'AATT_C
Cuts at: 2911 3289 2911
Size: 378 5224
FspI TGC'GCA
Cuts at: 4108 4108
Size: 5602
HaeII r_GCGC'y
Cuts at: 1678 2192 2282 4749 1678
Size: 514 90 2467 2531
Fragments arranged by size:
2531 2467 514 90
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2276 2308 2609 2641 2762
Size: 32 135 32 704 32 301 32 121
Cuts at: 2762 2794 4243 4275 4317 4349 1373
Size: 32 1449 32 42 32 2626
Fragments arranged by size:
2626 1449 704 301 135 121 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2946 3192 669
Size: 988 281 1008 246 3079
Fragments arranged by size:
3079 1008 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5602
HpaI GTT'AAC
Cuts at: 3192 3192
Size: 5602
KpnI G_GTAC'C
Cuts at: 2927 2927
Size: 5602
MfeI C'AATT_G
Cuts at: 3201 3201
Size: 5602
MluI A'CGCG_T
Cuts at: 5478 5478
Size: 5602
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4716 4900 1305
Size: 3411 184 2007
Fragments arranged by size:
3411 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3780
Fragments arranged by size:
3780 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3560 3919 4078 518
Size: 648 2394 359 159 2042
Fragments arranged by size:
2394 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2043 2069 2715 3018 3695 4843 5088 1606
Size: 437 26 646 303 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 646 437 303 245 26
NcoI C'CATG_G
Cuts at: 513 1065 2004 2136 513
Size: 552 939 132 3979
Fragments arranged by size:
3979 939 552 132
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5602
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5602
NotI GC'GGCC_GC
Cuts at: 3049 3049
Size: 5602
NsiI A_TGCA'T
Cuts at: 2389 2389
Size: 5602
NspI r_CATG'y
Cuts at: 1132 1229 2320 2960 4505 1132
Size: 97 1091 640 1545 2229
Fragments arranged by size:
2229 1545 1091 640 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5602
PciI A'CATG_T
Cuts at: 4501 4501
Size: 5602
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5602
PfoI T'CCnGG_A
Cuts at: 2736 2868 2736
Size: 132 5470
PmeI GTTT'AAAC
Cuts at: 2906 2906
Size: 5602
PmlI CAC'GTG
Cuts at: 2674 2674
Size: 5602
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2669 2701 3671 3703 5184 5216 2669
Size: 32 970 32 1481 32 3055
Fragments arranged by size:
3055 1481 970 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2832 2832
Size: 5602
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5602
PsiI TTA'TAA
Cuts at: 3172 3172
Size: 5602
PspOMI G'GGCC_C
Cuts at: 2119 2131 2119
Size: 12 5590
PstI C_TGCA'G
Cuts at: 830 2466 2604 2954 830
Size: 1636 138 350 3478
Fragments arranged by size:
3478 1636 350 138
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5602
PvuII CAG'CTG
Cuts at: 2715 2715
Size: 5602
SacII CC_GC'GG
Cuts at: 2070 2070
Size: 5602
SalI G'TCGA_C
Cuts at: 1936 2944 1936
Size: 1008 4594
SanDI GG'GwC_CC
Cuts at: 2832 2832
Size: 5602
SbfI CC_TGCA'GG
Cuts at: 2954 2954
Size: 5602
ScaI AGT'ACT
Cuts at: 3850 5562 3850
Size: 1712 3890
SfcI C'TryA_G
Cuts at: 826 1045 2462 2600 2950 4085 4766 4957 826
Size: 219 1417 138 350 1135 681 191 1471
Fragments arranged by size:
1471 1417 1135 681 350 219 191 138
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5602
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5602
SmaI CCC'GGG
Cuts at: 1872 2479 2561 2929 1872
Size: 607 82 368 4545
Fragments arranged by size:
4545 607 368 82
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3706 4607 4869 820
Size: 197 926 1763 901 262 1553
Fragments arranged by size:
1763 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3393 493
Size: 2900 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5602
SphI G_CATG'C
Cuts at: 1132 2320 2960 1132
Size: 1188 640 3774
Fragments arranged by size:
3774 1188 640
SspI AAT'ATT
Cuts at: 5 52 3526 5
Size: 47 3474 2081
Fragments arranged by size:
3474 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5602
StyI C'CwwG_G
Cuts at: 513 1065 2004 2136 2550 3041 513
Size: 552 939 132 414 491 3074
Fragments arranged by size:
3074 939 552 491 414 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2426 3629 3646 3799 3984 4429 325
Size: 1255 846 1203 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1203 846 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3848 5560 96
Size: 274 80 33 51 1443 1871 1712 138
Fragments arranged by size:
1871 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5562 5594 5562
Size: 32 5570
Tth111I GACn'n_nGTC
Cuts at: 1316 2267 1316
Size: 951 4651
XbaI T'CTAG_A
Cuts at: 2938 2938
Size: 5602
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5602
XmaI C'CCGG_G
Cuts at: 1870 2477 2559 2927 1870
Size: 607 82 368 4545
Fragments arranged by size:
4545 607 368 82
XmnI GAAnn'nnTTC
Cuts at: 3731 3731
Size: 5602
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3410 276
Size: 53 83 186 936 1876 2468
Fragments arranged by size:
2468 1876 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BplI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI PacI PspXI PsrI RsrII SacI SapI SexAI
SfiI SfoI SrfI SwaI XcmI