Restriction map for
pFN21AE9577
(Circular) (Six-base) MAPSORT of: pFN21AE9577.seq Check: 5864 from: 1 to: 6334
pFN21AE9577 6334 bp
With 182 enzymes: SgfI *
January 30, 2014 17:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3018 3391 3018
Size: 373 5961
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4144 278
Size: 53 83 186 936 2608 2468
Fragments arranged by size:
2608 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3369 3677 1937
Size: 1432 308 4594
Fragments arranged by size:
4594 1432 308
Acc65I G'GTAC_C
Cuts at: 3655 3655
Size: 6334
AclI AA'CG_TT
Cuts at: 1563 4461 4834 1563
Size: 2898 373 3063
Fragments arranged by size:
3063 2898 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2602 3035 3347 4397 5781 6049 1632
Size: 970 433 312 1050 1384 268 1917
Fragments arranged by size:
1917 1384 1050 970 433 312 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6137
AflIII A'CryG_T
Cuts at: 1203 2859 5233 6210 1203
Size: 1656 2374 977 1327
Fragments arranged by size:
2374 1656 1327 977
AgeI A'CCGG_T
Cuts at: 5165 5165
Size: 6334
AhdI GACnn_n'nnGTC
Cuts at: 5063 5063
Size: 6334
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6334
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6334
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3194 3226 3563 3595 3194
Size: 32 337 32 5933
Fragments arranged by size:
5933 337 32 32
AlwNI CAG_nnn'CTG
Cuts at: 3133 3430 4077 5649 3133
Size: 297 647 1572 3818
Fragments arranged by size:
3818 1572 647 297
ApaLI G'TGCA_C
Cuts at: 4391 5547 4391
Size: 1156 5178
ApoI r'AATT_y
Cuts at: 1493 3643 3869 4021 6130 1493
Size: 2150 226 152 2109 1697
Fragments arranged by size:
2150 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4888 160
Size: 872 3856 1606
Fragments arranged by size:
3856 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6334
AvaI C'yCGr_G
Cuts at: 1870 1943 2298 2426 3659 1870
Size: 73 355 128 1233 4545
Fragments arranged by size:
4545 1233 355 128 73
BamHI G'GATC_C
Cuts at: 3664 4031 3664
Size: 367 5967
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3020 3176 3236 3655 5110
Size: 325 217 627 1233 156 60 419 1455
Cuts at: 5110 618
Size: 1842
Fragments arranged by size:
1842 1455 1233 627 419 325 217 156
60
BanII G_rGCy'C
Cuts at: 1391 2031 2045 2433 2463 3455 3653 1391
Size: 640 14 388 30 992 198 4072
Fragments arranged by size:
4072 992 640 388 198 30 14
BbeI G_GCGC'C
Cuts at: 3024 3180 3024
Size: 156 6178
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2414 2863 928
Size: 971 515 449 4399
Fragments arranged by size:
4399 971 515 449
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2966 3000 4525 4559 1903
Size: 34 1029 34 1525 34 3678
Fragments arranged by size:
3678 1525 1029 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4226 5436 4226
Size: 1210 5124
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6334
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3143 4945 136
Size: 107 122 71 631 605 1471 1802 1525
Fragments arranged by size:
1802 1525 1471 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6329 6329
Size: 6334
BlpI GC'TnA_GC
Cuts at: 3751 3751
Size: 6334
Bme1580I G_kGCm'C
Cuts at: 1790 3414 4395 5551 1790
Size: 1624 981 1156 2573
Fragments arranged by size:
2573 1624 1156 981
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6334
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4065 5023 442
Size: 416 53 83 399 24 2648 958 1753
Fragments arranged by size:
2648 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6334
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2937 2937
Size: 6334
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2204 2306 3260 4994 1239
Size: 965 102 954 1734 2579
Fragments arranged by size:
2579 1734 965 954 102
Bpu10I CC'TnA_GC
Cuts at: 2591 3055 2591
Size: 464 5870
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4459 5324 5622 4459
Size: 865 298 5171
Fragments arranged by size:
5171 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4997 882
Size: 617 3498 2219
Fragments arranged by size:
3498 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2629 2862 3190 4125 493
Size: 711 1425 233 328 935 2702
Fragments arranged by size:
2702 1425 935 711 328 233
BsaBI GATnn'nnATC
Cuts at: 3116 6328 3116
Size: 3212 3122
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3021 3177 4141 4523
Size: 53 83 186 936 1488 156 964 382
Cuts at: 4523 5158 275
Size: 635 1451
Fragments arranged by size:
1488 1451 964 936 635 382 186 156
83 53
BsaWI w'CCGG_w
Cuts at: 4767 5165 5439 5586 5997 4767
Size: 398 274 147 411 5104
Fragments arranged by size:
5104 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2177 2207 2445 2475 3194
Size: 30 165 30 774 30 238 30 719
Cuts at: 3194 3224 1178
Size: 30 4288
Fragments arranged by size:
4288 774 719 238 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2035 2423 2915 3017 3554 1186
Size: 849 388 492 102 537 3966
Fragments arranged by size:
3966 849 537 492 388 102
BseYI C'CCAG_C
Cuts at: 2209 3060 5537 6033 2209
Size: 851 2477 496 2510
Fragments arranged by size:
2510 2477 851 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5761
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2035 3784 4545 5573 1540
Size: 275 186 34 1749 761 1028 2301
Fragments arranged by size:
2301 1749 1028 761 275 186 34
BsiHKAI G_wGCw'C
Cuts at: 3477 4395 4480 5551 3477
Size: 918 85 1071 4260
Fragments arranged by size:
4260 1071 918 85
BsmI GAATG_Cn'
Cuts at: 3845 3938 3845
Size: 93 6241
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2031 2045 2433 2463 3414 3455 3477
Size: 399 241 14 388 30 951 41 22
Cuts at: 3477 3653 4395 4480 5551 1391
Size: 176 742 85 1071 2174
Fragments arranged by size:
2174 1071 951 742 399 388 241 176
85 41 30 22 14
BspEI T'CCGG_A
Cuts at: 5997 5997
Size: 6334
BspHI T'CATG_A
Cuts at: 4223 5953 4223
Size: 1730 4604
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2368 3018 3118 3391 3525 3689 844
Size: 1524 650 100 273 134 164 3489
Fragments arranged by size:
3489 1524 650 273 164 134 100
BsrBI CCG'CTC
Cuts at: 1394 1823 2138 4221 1394
Size: 429 315 2083 3507
Fragments arranged by size:
3507 2083 429 315
BsrDI GCAATG_nn'
Cuts at: 65 1248 4829 5003 65
Size: 1183 3581 174 1396
Fragments arranged by size:
3581 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2109 4978 5165 1700
Size: 110 299 2869 187 2869
Fragments arranged by size:
2869 2869 299 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6334
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6334
BssSI C'ACGA_G
Cuts at: 4394 5406 4394
Size: 1012 5322
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6334
BstBI TT'CG_AA
Cuts at: 5138 5138
Size: 6334
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6334
BstYI r'GATC_y
Cuts at: 1972 2371 2877 3664 4031 4416 4433 5885 5971
Size: 399 506 787 367 385 17 1452 86
Cuts at: 5971 5983 6329 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 787 506 399 385 367 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2035 2046 513
Size: 552 939 31 11 4801
Fragments arranged by size:
4801 939 552 31 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2986 3025 3370 487
Size: 683 56 1760 39 345 3451
Fragments arranged by size:
3451 1760 683 345 56 39
BtsI GCAGTG_nn'
Cuts at: 2016 2550 3315 3431 3850 4644 4664 2016
Size: 534 765 116 419 794 20 3686
Fragments arranged by size:
3686 794 765 534 419 116 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6334
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6299
DraI TTT'AAA
Cuts at: 3638 3638
Size: 6334
DraIII CAC_nnn'GTG
Cuts at: 2632 2632
Size: 6334
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5341 809
Size: 507 4025 1802
Fragments arranged by size:
4025 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2032 2820 3135
Size: 54 1425 50 275 18 202 788 315
Cuts at: 3135 3318 3781 4670 6299 8
Size: 183 463 889 1629 43
Fragments arranged by size:
1629 1425 889 788 463 315 275 202
183 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2032 3781 1537
Size: 275 220 1749 4090
Fragments arranged by size:
4090 1749 275 220
EarI CTCTTCn'nnn_
Cuts at: 1353 2223 2933 3035 4264 1353
Size: 870 710 102 1229 3423
Fragments arranged by size:
3423 1229 870 710 102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2517 2663 2797 4921 5295 5441
Size: 1064 138 928 146 134 2124 374 146
Cuts at: 5441 387
Size: 1280
Fragments arranged by size:
2124 1280 1064 928 374 146 146 138
134
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2204 2306 2602 3035 3260 3347 4397
Size: 393 572 102 296 433 225 87 1050
Cuts at: 4397 4994 5781 6049 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1050 787 597 572 433 393 296
268 225 102 87
EcoNI CCTnn'n_nnAGG
Cuts at: 2219 3014 3398 2219
Size: 795 384 5155
Fragments arranged by size:
5155 795 384
EcoO109I rG'GnC_Cy
Cuts at: 2508 2555 2575 3130 2508
Size: 47 20 555 5712
Fragments arranged by size:
5712 555 47 20
EcoRI G'AATT_C
Cuts at: 3643 4021 3643
Size: 378 5956
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3121 3121
Size: 6334
FspI TGC'GCA
Cuts at: 4840 4840
Size: 6334
HaeII r_GCGC'y
Cuts at: 1678 2120 3024 3180 5481 1678
Size: 442 904 156 2301 2531
Fragments arranged by size:
2531 2301 904 442 156
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2127 2159 2539 2571 2626
Size: 32 135 32 555 32 380 32 55
Cuts at: 2626 2658 4975 5007 5049 5081 1373
Size: 32 2317 32 42 32 2626
Fragments arranged by size:
2626 2317 555 380 135 55 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2535 3604 3678 3924 669
Size: 988 281 597 1069 74 246 3079
Fragments arranged by size:
3079 1069 988 597 281 246 74
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6334
HpaI GTT'AAC
Cuts at: 3924 3924
Size: 6334
KasI G'GCGC_C
Cuts at: 3020 3176 3020
Size: 156 6178
KpnI G_GTAC'C
Cuts at: 3659 3659
Size: 6334
MfeI C'AATT_G
Cuts at: 3933 3933
Size: 6334
MluI A'CGCG_T
Cuts at: 6210 6210
Size: 6334
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3066 5448 5632 1305
Size: 1761 2382 184 2007
Fragments arranged by size:
2382 2007 1761 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3137 3320 10
Size: 54 1425 343 1305 183 3024
Fragments arranged by size:
3024 1425 1305 343 183 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2726 2729 2801 3345 4292 4651
Size: 648 843 717 3 72 544 947 359
Cuts at: 4651 4810 518
Size: 159 2042
Fragments arranged by size:
2042 947 843 717 648 544 359 159
72 3
MspA1I CmG'CkG
Cuts at: 1606 2037 2048 2062 2149 2771 2825 3523 3618
Size: 431 11 14 87 622 54 698 95
Cuts at: 3618 3750 4427 5575 5820 1606
Size: 132 677 1148 245 2120
Fragments arranged by size:
2120 1148 698 677 622 431 245 132
95 87 54 14 11
NarI GG'CG_CC
Cuts at: 3021 3177 3021
Size: 156 6178
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4843
Fragments arranged by size:
4843 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6334
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6334
NotI GC'GGCC_GC
Cuts at: 3781 3781
Size: 6334
NspI r_CATG'y
Cuts at: 1132 1229 3692 5237 1132
Size: 97 2463 1545 2229
Fragments arranged by size:
2463 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6334
PciI A'CATG_T
Cuts at: 5233 5233
Size: 6334
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2113 3442 1108
Size: 1005 1329 4000
Fragments arranged by size:
4000 1329 1005
PfoI T'CCnGG_A
Cuts at: 2217 2307 2436 2736 3276 3582 2217
Size: 90 129 300 540 306 4969
Fragments arranged by size:
4969 540 306 300 129 90
PmeI GTTT'AAAC
Cuts at: 3638 3638
Size: 6334
PmlI CAC'GTG
Cuts at: 2629 2629
Size: 6334
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3194 3226 4403 4435 5916 5948 3194
Size: 32 1177 32 1481 32 3580
Fragments arranged by size:
3580 1481 1177 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2508 2555 2575 3130 2508
Size: 47 20 555 5712
Fragments arranged by size:
5712 555 47 20
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6334
PsiI TTA'TAA
Cuts at: 3904 3904
Size: 6334
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3497 3529 3497
Size: 32 6302
PstI C_TGCA'G
Cuts at: 830 2379 2518 2855 3402 3522 3686 830
Size: 1549 139 337 547 120 164 3478
Fragments arranged by size:
3478 1549 547 337 164 139 120
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6334
PvuII CAG'CTG
Cuts at: 2149 3523 2149
Size: 1374 4960
SacII CC_GC'GG
Cuts at: 2038 2049 2038
Size: 11 6323
SalI G'TCGA_C
Cuts at: 1936 3676 1936
Size: 1740 4594
SbfI CC_TGCA'GG
Cuts at: 3686 3686
Size: 6334
ScaI AGT'ACT
Cuts at: 4582 6294 4582
Size: 1712 4622
SexAI A'CCwGG_T
Cuts at: 2577 2577
Size: 6334
SfcI C'TryA_G
Cuts at: 826 1045 2375 2514 2851 3398 3518 3682 4817
Size: 219 1330 139 337 547 120 164 1135
Cuts at: 4817 5498 5689 826
Size: 681 191 1471
Fragments arranged by size:
1471 1330 1135 681 547 337 219 191
164 139 120
SfiI GGCCn_nnn'nGGCC
Cuts at: 3143 3143
Size: 6334
SfoI GGC'GCC
Cuts at: 3022 3178 3022
Size: 156 6178
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6334
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6334
SmaI CCC'GGG
Cuts at: 1872 2300 2428 3661 1872
Size: 428 128 1233 4545
Fragments arranged by size:
4545 1233 428 128
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4438 5339 5601 820
Size: 197 926 2495 901 262 1553
Fragments arranged by size:
2495 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4125 493
Size: 3632 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6334
SphI G_CATG'C
Cuts at: 1132 3692 1132
Size: 2560 3774
SspI AAT'ATT
Cuts at: 5 52 4258 5
Size: 47 4206 2081
Fragments arranged by size:
4206 2081 47
StuI AGG'CCT
Cuts at: 1517 2982 3591 1517
Size: 1465 609 4260
Fragments arranged by size:
4260 1465 609
StyI C'CwwG_G
Cuts at: 513 1065 2004 2367 2898 3773 513
Size: 552 939 363 531 875 3074
Fragments arranged by size:
3074 939 875 552 531 363
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2075 2259 2827 4361 4378 4531 4716
Size: 1255 495 184 568 1534 17 153 185
Cuts at: 4716 5161 325
Size: 445 1498
Fragments arranged by size:
1534 1498 1255 568 495 445 185 184
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2661 2994 4580
Size: 274 80 33 51 1443 684 333 1586
Cuts at: 4580 6292 96
Size: 1712 138
Fragments arranged by size:
1712 1586 1443 684 333 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6294 6326 6294
Size: 32 6302
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6334
XbaI T'CTAG_A
Cuts at: 3670 3670
Size: 6334
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2179 3130 3284 2179
Size: 951 154 5229
Fragments arranged by size:
5229 951 154
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6334
XmaI C'CCGG_G
Cuts at: 1870 2298 2426 3659 1870
Size: 428 128 1233 4545
Fragments arranged by size:
4545 1233 428 128
XmnI GAAnn'nnTTC
Cuts at: 2156 3332 4463 2156
Size: 1176 1131 4027
Fragments arranged by size:
4027 1176 1131
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4142 276
Size: 53 83 186 936 2608 2468
Fragments arranged by size:
2608 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI PvuII SacII SalI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BsiWI
BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI
NaeI NgoMIV NruI NsiI PacI PspOMI PspXI RsrII
SacI SanDI SapI SrfI SwaI