Restriction map for
pFN21AE9573
(Circular) (Six-base) MAPSORT of: pFN21AE9573.seq Check: 908 from: 1 to: 6127
pFN21AE9573 6127 bp
With 182 enzymes: SgfI *
September 11, 2018 19:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3937 278
Size: 53 83 186 936 2401 2468
Fragments arranged by size:
2468 2401 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3470 1937
Size: 1533 4594
Acc65I G'GTAC_C
Cuts at: 3448 3448
Size: 6127
AclI AA'CG_TT
Cuts at: 1563 4254 4627 1563
Size: 2691 373 3063
Fragments arranged by size:
3063 2691 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2450 3041 4190 5574 5842 1632
Size: 818 591 1149 1384 268 1917
Fragments arranged by size:
1917 1384 1149 818 591 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5930
AflIII A'CryG_T
Cuts at: 1203 2562 3012 5026 6003 1203
Size: 1359 450 2014 977 1327
Fragments arranged by size:
2014 1359 1327 977 450
AgeI A'CCGG_T
Cuts at: 4958 4958
Size: 6127
AhdI GACnn_n'nnGTC
Cuts at: 4856 4856
Size: 6127
AleI CACnn'nnGTG
Cuts at: 1166 2492 1166
Size: 1326 4801
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2684 1184
Size: 1500 4627
AlwNI CAG_nnn'CTG
Cuts at: 3296 3343 3870 5442 3296
Size: 47 527 1572 3981
Fragments arranged by size:
3981 1572 527 47
ApaI G_GGCC'C
Cuts at: 3226 3226
Size: 6127
ApaLI G'TGCA_C
Cuts at: 4184 5340 4184
Size: 1156 4971
ApoI r'AATT_y
Cuts at: 1493 2584 3234 3436 3662 3814 5923 1493
Size: 1091 650 202 226 152 2109 1697
Fragments arranged by size:
2109 1697 1091 650 226 202 152
AseI AT'TA_AT
Cuts at: 160 1032 4681 160
Size: 872 3649 1606
Fragments arranged by size:
3649 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6127
AvaI C'yCGr_G
Cuts at: 1870 1943 3452 1870
Size: 73 1509 4545
Fragments arranged by size:
4545 1509 73
BamHI G'GATC_C
Cuts at: 3457 3824 3457
Size: 367 5760
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2818 3261 3448 4903 618
Size: 325 217 627 1031 443 187 1455 1842
Fragments arranged by size:
1842 1455 1031 627 443 325 217 187
BanII G_rGCy'C
Cuts at: 1391 2112 2637 3226 3446 1391
Size: 721 525 589 220 4072
Fragments arranged by size:
4072 721 589 525 220
BbeI G_GCGC'C
Cuts at: 3265 3265
Size: 6127
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5156
BbvCI CC'TCA_GC
Cuts at: 3344 3344
Size: 6127
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3362 3396 4318 4352 1903
Size: 34 1425 34 922 34 3678
Fragments arranged by size:
3678 1425 922 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4019 5229 4019
Size: 1210 4917
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6127
BfrBI ATG'CAT
Cuts at: 2438 2438
Size: 6127
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4738 136
Size: 107 122 71 631 605 3066 1525
Fragments arranged by size:
3066 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6122 6122
Size: 6127
BlpI GC'TnA_GC
Cuts at: 3544 3544
Size: 6127
Bme1580I G_kGCm'C
Cuts at: 1790 3054 3226 4188 5344 1790
Size: 1264 172 962 1156 2573
Fragments arranged by size:
2573 1264 1156 962 172
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6127
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3858 4816 442
Size: 416 53 83 399 24 2441 958 1753
Fragments arranged by size:
2441 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6127
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2286 3106 4787 1239
Size: 1047 820 1681 2579
Fragments arranged by size:
2579 1681 1047 820
Bpu10I CC'TnA_GC
Cuts at: 3344 3344
Size: 6127
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4252 5117 5415 4252
Size: 865 298 4964
Fragments arranged by size:
4964 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3082 4790 882
Size: 617 1583 1708 2219
Fragments arranged by size:
2219 1708 1583 617
BsaAI yAC'GTr
Cuts at: 493 1204 3127 3918 493
Size: 711 1923 791 2702
Fragments arranged by size:
2702 1923 791 711
BsaBI GATnn'nnATC
Cuts at: 2310 2597 6121 2310
Size: 287 3524 2316
Fragments arranged by size:
3524 2316 287
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3262 3934 4316 4951
Size: 53 83 186 936 1729 672 382 635
Cuts at: 4951 275
Size: 1451
Fragments arranged by size:
1729 1451 936 672 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2302 4560 4958 5232 5379 5790 2302
Size: 2258 398 274 147 411 2639
Fragments arranged by size:
2639 2258 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2622 2652 1178
Size: 30 165 30 1219 30 4653
Fragments arranged by size:
4653 1219 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3389 1186
Size: 2203 3924
BseYI C'CCAG_C
Cuts at: 2247 2802 3058 5330 5826 2247
Size: 555 256 2272 496 2548
Fragments arranged by size:
2548 2272 555 496 256
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2537 1163
Size: 573 801 4753
Fragments arranged by size:
4753 801 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3577 4338 5366 1540
Size: 275 186 1576 761 1028 2301
Fragments arranged by size:
2301 1576 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2637 4188 4273 5344 2637
Size: 1551 85 1071 3420
Fragments arranged by size:
3420 1551 1071 85
BsmI GAATG_Cn'
Cuts at: 2184 2440 3638 3731 2184
Size: 256 1198 93 4580
Fragments arranged by size:
4580 1198 256 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2112 2637 3054 3226 3446 4188 4273
Size: 399 322 525 417 172 220 742 85
Cuts at: 4273 5344 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 525 417 399 322 220
172 85
BspEI T'CCGG_A
Cuts at: 2302 5790 2302
Size: 3488 2639
BspHI T'CATG_A
Cuts at: 4016 5746 4016
Size: 1730 4397
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3482 844
Size: 2638 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3276 4014 1394
Size: 429 1453 738 3507
Fragments arranged by size:
3507 1453 738 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2413 4622 4796 65
Size: 1183 1165 2209 174 1396
Fragments arranged by size:
2209 1396 1183 1165 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2025 2260 4771 4958 1700
Size: 110 215 235 2511 187 2869
Fragments arranged by size:
2869 2511 235 215 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6127
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6127
BssSI C'ACGA_G
Cuts at: 2636 4187 5199 2636
Size: 1551 1012 3564
Fragments arranged by size:
3564 1551 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2779 3296 794
Size: 1985 517 3625
Fragments arranged by size:
3625 1985 517
BstBI TT'CG_AA
Cuts at: 4931 4931
Size: 6127
BstEII G'GTnAC_C
Cuts at: 1184 2545 1184
Size: 1361 4766
BstYI r'GATC_y
Cuts at: 1972 2727 3457 3824 4209 4226 5678 5764 5776
Size: 755 730 367 385 17 1452 86 12
Cuts at: 5776 6122 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 755 730 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2908 513
Size: 552 1843 3732
Fragments arranged by size:
3732 1843 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5388
Fragments arranged by size:
5388 683 56
BtsI GCAGTG_nn'
Cuts at: 3643 4437 4457 3643
Size: 794 20 5313
Fragments arranged by size:
5313 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6127
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6092
DraI TTT'AAA
Cuts at: 3431 3431
Size: 6127
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5134 809
Size: 507 3818 1802
Fragments arranged by size:
3818 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3574 4463 6092
Size: 54 1425 50 275 18 1744 889 1629
Cuts at: 6092 8
Size: 43
Fragments arranged by size:
1744 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3574 1537
Size: 275 1762 4090
Fragments arranged by size:
4090 1762 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4057 1353
Size: 2704 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3017 4714 5088 5234 387
Size: 1064 138 1428 1697 374 146 1280
Fragments arranged by size:
1697 1428 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2635 2635
Size: 6127
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2286 2450 3041 3106 4190 4787 5574
Size: 393 654 164 591 65 1084 597 787
Cuts at: 5574 5842 1239
Size: 268 1524
Fragments arranged by size:
1524 1084 787 654 597 591 393 268
164 65
EcoO109I rG'GnC_Cy
Cuts at: 2647 2647
Size: 6127
EcoRI G'AATT_C
Cuts at: 2584 3436 3814 2584
Size: 852 378 4897
Fragments arranged by size:
4897 852 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2794 2794
Size: 6127
FspI TGC'GCA
Cuts at: 4633 4633
Size: 6127
HaeII r_GCGC'y
Cuts at: 1678 3217 3265 5274 1678
Size: 1539 48 2009 2531
Fragments arranged by size:
2531 2009 1539 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2990 3022 3121 3153 3401
Size: 32 135 32 1418 32 99 32 248
Cuts at: 3401 3433 4768 4800 4842 4874 1373
Size: 32 1335 32 42 32 2626
Fragments arranged by size:
2626 1418 1335 248 135 99 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3471 3717 669
Size: 988 281 1533 246 3079
Fragments arranged by size:
3079 1533 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6127
HpaI GTT'AAC
Cuts at: 3717 3717
Size: 6127
KasI G'GCGC_C
Cuts at: 3261 3261
Size: 6127
KpnI G_GTAC'C
Cuts at: 3452 3452
Size: 6127
MfeI C'AATT_G
Cuts at: 3726 3726
Size: 6127
MluI A'CGCG_T
Cuts at: 6003 6003
Size: 6127
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2123 2415 3120 3294 5241 5425 1305
Size: 818 292 705 174 1947 184 2007
Fragments arranged by size:
2007 1947 818 705 292 184 174
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4305
Fragments arranged by size:
4305 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2492 2750 4085 4444 4603 518
Size: 648 1326 258 1335 359 159 2042
Fragments arranged by size:
2042 1335 1326 648 359 258 159
MspA1I CmG'CkG
Cuts at: 1606 2815 3296 3543 4220 5368 5613 1606
Size: 1209 481 247 677 1148 245 2120
Fragments arranged by size:
2120 1209 1148 677 481 247 245
NarI GG'CG_CC
Cuts at: 3262 3262
Size: 6127
NcoI C'CATG_G
Cuts at: 513 1065 2908 513
Size: 552 1843 3732
Fragments arranged by size:
3732 1843 552
NdeI CA'TA_TG
Cuts at: 387 2440 387
Size: 2053 4074
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6127
NotI GC'GGCC_GC
Cuts at: 3574 3574
Size: 6127
NsiI A_TGCA'T
Cuts at: 2440 2440
Size: 6127
NspI r_CATG'y
Cuts at: 1132 1229 2566 3016 3485 5030 1132
Size: 97 1337 450 469 1545 2229
Fragments arranged by size:
2229 1545 1337 469 450 97
PasI CC'CwG_GG
Cuts at: 1348 2503 1348
Size: 1155 4972
PciI A'CATG_T
Cuts at: 2562 3012 5026 2562
Size: 450 2014 3663
Fragments arranged by size:
3663 2014 450
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6127
PmeI GTTT'AAAC
Cuts at: 3431 3431
Size: 6127
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4196 4228 5709 5741 4196
Size: 32 1481 32 4582
Fragments arranged by size:
4582 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2647 2647
Size: 6127
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6127
PsiI TTA'TAA
Cuts at: 3697 3697
Size: 6127
PspOMI G'GGCC_C
Cuts at: 3222 3222
Size: 6127
PstI C_TGCA'G
Cuts at: 830 3292 3479 830
Size: 2462 187 3478
Fragments arranged by size:
3478 2462 187
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6127
SacI G_AGCT'C
Cuts at: 2637 2637
Size: 6127
SalI G'TCGA_C
Cuts at: 1936 3469 1936
Size: 1533 4594
SanDI GG'GwC_CC
Cuts at: 2647 2647
Size: 6127
SbfI CC_TGCA'GG
Cuts at: 3479 3479
Size: 6127
ScaI AGT'ACT
Cuts at: 4375 6087 4375
Size: 1712 4415
SexAI A'CCwGG_T
Cuts at: 2384 2607 2384
Size: 223 5904
SfcI C'TryA_G
Cuts at: 826 1045 3288 3475 4610 5291 5482 826
Size: 219 2243 187 1135 681 191 1471
Fragments arranged by size:
2243 1471 1135 681 219 191 187
SfoI GGC'GCC
Cuts at: 3263 3263
Size: 6127
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6127
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6127
SmaI CCC'GGG
Cuts at: 1872 3454 1872
Size: 1582 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4231 5132 5394 820
Size: 197 926 2288 901 262 1553
Fragments arranged by size:
2288 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3918 493
Size: 3425 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6127
SphI G_CATG'C
Cuts at: 1132 3485 1132
Size: 2353 3774
SspI AAT'ATT
Cuts at: 5 52 4051 5
Size: 47 3999 2081
Fragments arranged by size:
3999 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6127
StyI C'CwwG_G
Cuts at: 513 1065 2200 2530 2908 3367 3566 513
Size: 552 1135 330 378 459 199 3074
Fragments arranged by size:
3074 1135 552 459 378 330 199
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2073 2190 4154 4171 4324 4509 4954
Size: 1255 493 117 1964 17 153 185 445
Cuts at: 4954 325
Size: 1498
Fragments arranged by size:
1964 1498 1255 493 445 185 153 117
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2160 2448 2685
Size: 274 80 33 51 1443 183 288 237
Cuts at: 2685 2853 4373 6085 96
Size: 168 1520 1712 138
Fragments arranged by size:
1712 1520 1443 288 274 237 183 168
138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2622 2654 2704 2736 6087 6119 2622
Size: 32 50 32 3351 32 2630
Fragments arranged by size:
3351 2630 50 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6127
XbaI T'CTAG_A
Cuts at: 3463 3463
Size: 6127
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6127
XmaI C'CCGG_G
Cuts at: 1870 3452 1870
Size: 1582 4545
XmnI GAAnn'nnTTC
Cuts at: 4256 4256
Size: 6127
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3935 276
Size: 53 83 186 936 2401 2468
Fragments arranged by size:
2468 2401 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
SacI SalI SanDI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BplI BsiWI
BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI
FspAI NaeI NgoMIV NruI PacI PfoI PmlI PspXI
PsrI PvuII RsrII SacII SapI SfiI SrfI SwaI
XcmI