Restriction map for
pFN21AE9572
(Circular) (Six-base) MAPSORT of: pFN21AE9572.seq Check: 2861 from: 1 to: 5827
pFN21AE9572 5827 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2342 2375 2433 2342
Size: 33 58 5736
Fragments arranged by size:
5736 58 33
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3637 278
Size: 53 83 186 936 2101 2468
Fragments arranged by size:
2468 2101 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3170 1937
Size: 1233 4594
Acc65I G'GTAC_C
Cuts at: 3148 3148
Size: 5827
AclI AA'CG_TT
Cuts at: 1563 3954 4327 1563
Size: 2391 373 3063
Fragments arranged by size:
3063 2391 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2146 2534 3890 5274 5542 1632
Size: 514 388 1356 1384 268 1917
Fragments arranged by size:
1917 1384 1356 514 388 268
AfeI AGC'GCT
Cuts at: 2321 2455 2321
Size: 134 5693
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5630
AflIII A'CryG_T
Cuts at: 1203 4726 5703 1203
Size: 3523 977 1327
Fragments arranged by size:
3523 1327 977
AgeI A'CCGG_T
Cuts at: 4658 4658
Size: 5827
AhdI GACnn_n'nnGTC
Cuts at: 4556 4556
Size: 5827
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5827
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5827
AlwNI CAG_nnn'CTG
Cuts at: 3570 5142 3570
Size: 1572 4255
ApaI G_GGCC'C
Cuts at: 2901 2901
Size: 5827
ApaLI G'TGCA_C
Cuts at: 2399 3884 5040 2399
Size: 1485 1156 3186
Fragments arranged by size:
3186 1485 1156
ApoI r'AATT_y
Cuts at: 1493 3136 3362 3514 5623 1493
Size: 1643 226 152 2109 1697
Fragments arranged by size:
2109 1697 1643 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4381 160
Size: 872 3349 1606
Fragments arranged by size:
3349 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5827
AvaI C'yCGr_G
Cuts at: 1870 1943 2020 2558 2687 2851 3152 1870
Size: 73 77 538 129 164 301 4545
Fragments arranged by size:
4545 538 301 164 129 77 73
BamHI G'GATC_C
Cuts at: 3157 3524 3157
Size: 367 5460
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2024 2187 2681 3148 4603
Size: 325 217 627 237 163 494 467 1455
Cuts at: 4603 618
Size: 1842
Fragments arranged by size:
1842 1455 627 494 467 325 237 217
163
BanII G_rGCy'C
Cuts at: 1391 2901 3105 3146 1391
Size: 1510 204 41 4072
Fragments arranged by size:
4072 1510 204 41
BbeI G_GCGC'C
Cuts at: 2685 2685
Size: 5827
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2194 928
Size: 971 295 4561
Fragments arranged by size:
4561 971 295
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4018 4052 1903
Size: 34 2081 34 3678
Fragments arranged by size:
3678 2081 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3719 4929 3719
Size: 1210 4617
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5827
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2483 4438 136
Size: 107 122 71 631 605 811 1955 1525
Fragments arranged by size:
1955 1525 811 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5822 5822
Size: 5827
BlpI GC'TnA_GC
Cuts at: 3119 3244 3119
Size: 125 5702
Bme1580I G_kGCm'C
Cuts at: 1790 2403 2793 2901 2982 3888 5044 1790
Size: 613 390 108 81 906 1156 2573
Fragments arranged by size:
2573 1156 906 613 390 108 81
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5827
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3100 3558 4516
Size: 416 53 83 399 24 1683 458 958
Cuts at: 4516 442
Size: 1753
Fragments arranged by size:
1753 1683 958 458 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5827
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2336 2857 2909 4487 1239
Size: 1097 521 52 1578 2579
Fragments arranged by size:
2579 1578 1097 521 52
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3952 4817 5115 3952
Size: 865 298 4664
Fragments arranged by size:
4664 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4490 882
Size: 617 2991 2219
Fragments arranged by size:
2991 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2905 3618 493
Size: 711 1701 713 2702
Fragments arranged by size:
2702 1701 713 711
BsaBI GATnn'nnATC
Cuts at: 5821 5821
Size: 5827
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2682 3042 3634 4016
Size: 53 83 186 936 1149 360 592 382
Cuts at: 4016 4651 275
Size: 635 1451
Fragments arranged by size:
1451 1149 936 635 592 382 360 186
83 53
BsaWI w'CCGG_w
Cuts at: 2169 4260 4658 4932 5079 5490 2169
Size: 2091 398 274 147 411 2506
Fragments arranged by size:
2506 2091 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2291 2321 2765 2795 1178
Size: 30 165 30 888 30 444 30 4210
Fragments arranged by size:
4210 888 444 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5827
BseYI C'CCAG_C
Cuts at: 2344 2463 2893 2981 5030 5526 2344
Size: 119 430 88 2049 496 2645
Fragments arranged by size:
2645 2049 496 430 119 88
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5254
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3277 4038 5066 1540
Size: 275 186 1276 761 1028 2301
Fragments arranged by size:
2301 1276 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2403 2814 2843 3053 3888 3973 5044 2403
Size: 411 29 210 835 85 1071 3186
Fragments arranged by size:
3186 1071 835 411 210 85 29
BsmI GAATG_Cn'
Cuts at: 3338 3431 3338
Size: 93 5734
BsmBI CGTCTCn'nnnn_
Cuts at: 2023 2023
Size: 5827
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2403 2793 2814 2843 2901 2982 3053
Size: 399 613 390 21 29 58 81 71
Cuts at: 3053 3105 3146 3888 3973 5044 1391
Size: 52 41 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 613 399 390 85 81
71 58 52 41 29 21
BspEI T'CCGG_A
Cuts at: 2169 5490 2169
Size: 3321 2506
BspHI T'CATG_A
Cuts at: 3716 5446 3716
Size: 1730 4097
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2238 2342 2349 2375 2433 3182 844
Size: 1394 104 7 26 58 749 3489
Fragments arranged by size:
3489 1394 749 104 58 26 7
BsrBI CCG'CTC
Cuts at: 1394 1823 3714 1394
Size: 429 1891 3507
Fragments arranged by size:
3507 1891 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2146 4322 4496 65
Size: 1183 898 2176 174 1396
Fragments arranged by size:
2176 1396 1183 898 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2459 4471 4658 1700
Size: 110 649 2012 187 2869
Fragments arranged by size:
2869 2012 649 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5827
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5827
BssSI C'ACGA_G
Cuts at: 3887 4899 3887
Size: 1012 4815
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2847 794
Size: 2053 3774
BstBI TT'CG_AA
Cuts at: 4631 4631
Size: 5827
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5827
BstXI CCAn_nnnn'nTGG
Cuts at: 2050 2110 2764 2050
Size: 60 654 5113
Fragments arranged by size:
5113 654 60
BstYI r'GATC_y
Cuts at: 1972 3157 3524 3909 3926 5378 5464 5476 5822
Size: 1185 367 385 17 1452 86 12 346
Cuts at: 5822 1972
Size: 1977
Fragments arranged by size:
1977 1452 1185 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2130 2183 2196 2593 513
Size: 552 939 126 53 13 397 3747
Fragments arranged by size:
3747 939 552 397 126 53 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5088
Fragments arranged by size:
5088 683 56
BtsI GCAGTG_nn'
Cuts at: 2982 3004 3343 4137 4157 2982
Size: 22 339 794 20 4652
Fragments arranged by size:
4652 794 339 22 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5827
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2056 2091 529
Size: 35 1492 35 4265
Fragments arranged by size:
4265 1492 35 35
DraI TTT'AAA
Cuts at: 3131 3131
Size: 5827
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4834 809
Size: 507 3518 1802
Fragments arranged by size:
3518 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2127 2133 2259
Size: 54 1425 50 275 18 297 6 126
Cuts at: 2259 2472 2484 2550 2724 2754 2970 3055 3274
Size: 213 12 66 174 30 216 85 219
Cuts at: 3274 4163 5792 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 297 275 219 216 213
174 126 85 66 54 50 43 30
18 12 6
EagI C'GGCC_G
Cuts at: 1537 1812 3274 1537
Size: 275 1462 4090
Fragments arranged by size:
4090 1462 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2333 2510 3757 1353
Size: 980 177 1247 3423
Fragments arranged by size:
3423 1247 980 177
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4414 4788 4934 387
Size: 1064 138 2825 374 146 1280
Fragments arranged by size:
2825 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2146 2336 2534 2857 2909 3890 4487
Size: 393 514 190 198 323 52 981 597
Cuts at: 4487 5274 5542 1239
Size: 787 268 1524
Fragments arranged by size:
1524 981 787 597 514 393 323 268
198 190 52
EcoO109I rG'GnC_Cy
Cuts at: 2467 2897 2898 2926 2467
Size: 430 1 28 5368
Fragments arranged by size:
5368 430 28 1
EcoRI G'AATT_C
Cuts at: 3136 3514 3136
Size: 378 5449
FspI TGC'GCA
Cuts at: 4333 4333
Size: 5827
HaeII r_GCGC'y
Cuts at: 1678 2182 2293 2323 2457 2685 2836 4974 1678
Size: 504 111 30 134 228 151 2138 2531
Fragments arranged by size:
2531 2138 504 228 151 134 111 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3092 3124 4468 4500 4542
Size: 32 135 32 1520 32 1344 32 42
Cuts at: 4542 4574 1373
Size: 32 2626
Fragments arranged by size:
2626 1520 1344 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3171 3417 669
Size: 988 281 1233 246 3079
Fragments arranged by size:
3079 1233 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5827
HpaI GTT'AAC
Cuts at: 3417 3417
Size: 5827
KasI G'GCGC_C
Cuts at: 2681 2681
Size: 5827
KpnI G_GTAC'C
Cuts at: 3152 3152
Size: 5827
MfeI C'AATT_G
Cuts at: 3426 3426
Size: 5827
MluI A'CGCG_T
Cuts at: 5703 5703
Size: 5827
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4941 5125 1305
Size: 3636 184 2007
Fragments arranged by size:
3636 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2135 2486 2552 2756 3057
Size: 54 1425 343 303 351 66 204 301
Cuts at: 3057 10
Size: 2780
Fragments arranged by size:
2780 1425 351 343 303 301 204 66
54
MslI CAynn'nnrTG
Cuts at: 518 1166 2108 2720 2807 3785 4144 4303 518
Size: 648 942 612 87 978 359 159 2042
Fragments arranged by size:
2042 978 942 648 612 359 159 87
MspA1I CmG'CkG
Cuts at: 1606 2057 2215 2257 2588 2656 2847 3027 3243
Size: 451 158 42 331 68 191 180 216
Cuts at: 3243 3920 5068 5313 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 451 331 245 216 191
180 158 68 42
NaeI GCC'GGC
Cuts at: 2461 2461
Size: 5827
NarI GG'CG_CC
Cuts at: 2682 2682
Size: 5827
NcoI C'CATG_G
Cuts at: 513 1065 2004 2183 513
Size: 552 939 179 4157
Fragments arranged by size:
4157 939 552 179
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5827
NgoMIV G'CCGG_C
Cuts at: 2459 2459
Size: 5827
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5827
NotI GC'GGCC_GC
Cuts at: 3274 3274
Size: 5827
NspI r_CATG'y
Cuts at: 1132 1229 3185 4730 1132
Size: 97 1956 1545 2229
Fragments arranged by size:
2229 1956 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5827
PciI A'CATG_T
Cuts at: 4726 4726
Size: 5827
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2286 2599 1108
Size: 1178 313 4336
Fragments arranged by size:
4336 1178 313
PmeI GTTT'AAAC
Cuts at: 3131 3131
Size: 5827
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3896 3928 5409 5441 3896
Size: 32 1481 32 4282
Fragments arranged by size:
4282 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2467 2467
Size: 5827
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5827
PsiI TTA'TAA
Cuts at: 3397 3397
Size: 5827
PspOMI G'GGCC_C
Cuts at: 2897 2897
Size: 5827
PstI C_TGCA'G
Cuts at: 830 2546 3179 830
Size: 1716 633 3478
Fragments arranged by size:
3478 1716 633
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5827
PvuII CAG'CTG
Cuts at: 2057 2215 3027 2057
Size: 158 812 4857
Fragments arranged by size:
4857 812 158
SalI G'TCGA_C
Cuts at: 1936 3169 1936
Size: 1233 4594
SbfI CC_TGCA'GG
Cuts at: 3179 3179
Size: 5827
ScaI AGT'ACT
Cuts at: 4075 5787 4075
Size: 1712 4115
SexAI A'CCwGG_T
Cuts at: 2913 2913
Size: 5827
SfcI C'TryA_G
Cuts at: 826 1045 2542 2608 3016 3175 4310 4991 5182
Size: 219 1497 66 408 159 1135 681 191
Cuts at: 5182 826
Size: 1471
Fragments arranged by size:
1497 1471 1135 681 408 219 191 159
66
SfoI GGC'GCC
Cuts at: 2683 2683
Size: 5827
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5827
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5827
SmaI CCC'GGG
Cuts at: 1872 3154 1872
Size: 1282 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3931 4832 5094 820
Size: 197 926 1988 901 262 1553
Fragments arranged by size:
1988 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3618 493
Size: 3125 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5827
SphI G_CATG'C
Cuts at: 1132 3185 1132
Size: 2053 3774
SspI AAT'ATT
Cuts at: 5 52 3751 5
Size: 47 3699 2081
Fragments arranged by size:
3699 2081 47
StuI AGG'CCT
Cuts at: 1517 2564 1517
Size: 1047 4780
StyI C'CwwG_G
Cuts at: 513 1065 2004 2183 3266 513
Size: 552 939 179 1083 3074
Fragments arranged by size:
3074 1083 939 552 179
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3854 3871 4024 4209 4654 325
Size: 1255 2274 17 153 185 445 1498
Fragments arranged by size:
2274 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4073 5785 96
Size: 274 80 33 51 1443 2096 1712 138
Fragments arranged by size:
2096 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2081 2113 2291 2323 5787 5819 2081
Size: 32 178 32 3464 32 2089
Fragments arranged by size:
3464 2089 178 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5827
XbaI T'CTAG_A
Cuts at: 3163 3163
Size: 5827
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2143 2884 2143
Size: 741 5086
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5827
XmaI C'CCGG_G
Cuts at: 1870 3152 1870
Size: 1282 4545
XmnI GAAnn'nnTTC
Cuts at: 3956 3956
Size: 5827
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3635 276
Size: 53 83 186 936 2101 2468
Fragments arranged by size:
2468 2101 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI
PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PfoI PmlI PspXI
PsrI RsrII SacI SacII SanDI SapI SfiI SrfI
SwaI