Restriction map for
pFN21AE9570
(Circular) (Six-base) MAPSORT of: pFN21AE9570.seq Check: 6749 from: 1 to: 5845
pFN21AE9570 5845 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3009 3655 278
Size: 53 83 186 936 1473 646 2468
Fragments arranged by size:
2468 1473 936 646 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2241 3188 1937
Size: 304 947 4594
Fragments arranged by size:
4594 947 304
Acc65I G'GTAC_C
Cuts at: 3166 3166
Size: 5845
AclI AA'CG_TT
Cuts at: 1563 3972 4345 1563
Size: 2409 373 3063
Fragments arranged by size:
3063 2409 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3908 5292 5560 1632
Size: 2276 1384 268 1917
Fragments arranged by size:
2276 1917 1384 268
AfeI AGC'GCT
Cuts at: 2704 2704
Size: 5845
AflII C'TTAA_G
Cuts at: 820 1017 2698 820
Size: 197 1681 3967
Fragments arranged by size:
3967 1681 197
AflIII A'CryG_T
Cuts at: 1203 2109 4744 5721 1203
Size: 906 2635 977 1327
Fragments arranged by size:
2635 1327 977 906
AgeI A'CCGG_T
Cuts at: 4676 4676
Size: 5845
AhdI GACnn_n'nnGTC
Cuts at: 4574 4574
Size: 5845
AleI CACnn'nnGTG
Cuts at: 1166 2051 1166
Size: 885 4960
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5845
AlwNI CAG_nnn'CTG
Cuts at: 2218 2873 3588 5160 2218
Size: 655 715 1572 2903
Fragments arranged by size:
2903 1572 715 655
ApaLI G'TGCA_C
Cuts at: 2161 3092 3902 5058 2161
Size: 931 810 1156 2948
Fragments arranged by size:
2948 1156 931 810
ApoI r'AATT_y
Cuts at: 1493 3154 3380 3532 5641 1493
Size: 1661 226 152 2109 1697
Fragments arranged by size:
2109 1697 1661 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4399 160
Size: 872 3367 1606
Fragments arranged by size:
3367 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5845
AvaI C'yCGr_G
Cuts at: 1870 1943 3170 1870
Size: 73 1227 4545
Fragments arranged by size:
4545 1227 73
AvrII C'CTAG_G
Cuts at: 2332 2332
Size: 5845
BamHI G'GATC_C
Cuts at: 3175 3542 3175
Size: 367 5478
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2173 3166 4621 618
Size: 325 217 627 386 993 1455 1842
Fragments arranged by size:
1842 1455 993 627 386 325 217
BanII G_rGCy'C
Cuts at: 1391 3164 1391
Size: 1773 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2553 3005 928
Size: 971 654 452 3768
Fragments arranged by size:
3768 971 654 452
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4036 4070 1903
Size: 34 2099 34 3678
Fragments arranged by size:
3678 2099 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3737 4947 3737
Size: 1210 4635
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5845
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2072 2714 4456
Size: 107 122 71 631 605 400 642 1742
Cuts at: 4456 136
Size: 1525
Fragments arranged by size:
1742 1525 642 631 605 400 122 107
71
BglII A'GATC_T
Cuts at: 5840 5840
Size: 5845
BlpI GC'TnA_GC
Cuts at: 3262 3262
Size: 5845
Bme1580I G_kGCm'C
Cuts at: 1790 2012 2165 3096 3906 5062 1790
Size: 222 153 931 810 1156 2573
Fragments arranged by size:
2573 1156 931 810 222 153
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5845
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2045 3576 4534
Size: 416 53 83 399 24 628 1531 958
Cuts at: 4534 442
Size: 1753
Fragments arranged by size:
1753 1531 958 628 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5845
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2468 3067 4505 1239
Size: 1229 599 1438 2579
Fragments arranged by size:
2579 1438 1229 599
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3970 4835 5133 3970
Size: 865 298 4682
Fragments arranged by size:
4682 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2549 4508 882
Size: 617 1050 1959 2219
Fragments arranged by size:
2219 1959 1050 617
BsaAI yAC'GTr
Cuts at: 493 1204 2929 3636 493
Size: 711 1725 707 2702
Fragments arranged by size:
2702 1725 711 707
BsaBI GATnn'nnATC
Cuts at: 5839 5839
Size: 5845
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3006 3652 4034 4669
Size: 53 83 186 936 1473 646 382 635
Cuts at: 4669 275
Size: 1451
Fragments arranged by size:
1473 1451 936 646 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2664 4278 4676 4950 5097 5508 2664
Size: 1614 398 274 147 411 3001
Fragments arranged by size:
3001 1614 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3032 3062 3065 3095 1178
Size: 30 165 30 1629 30 3 30 3928
Fragments arranged by size:
3928 1629 165 30 30 30 30 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2780 2838 3053 3149 1186
Size: 1594 58 215 96 3882
Fragments arranged by size:
3882 1594 215 96 58
BseYI C'CCAG_C
Cuts at: 2167 5048 5544 2167
Size: 2881 496 2468
Fragments arranged by size:
2881 2468 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5272
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2422 3295 4056 5084 1540
Size: 275 186 421 873 761 1028 2301
Fragments arranged by size:
2301 1028 873 761 421 275 186
BsiHKAI G_wGCw'C
Cuts at: 2165 3096 3906 3991 5062 2165
Size: 931 810 85 1071 2948
Fragments arranged by size:
2948 1071 931 810 85
BsmI GAATG_Cn'
Cuts at: 3356 3449 3356
Size: 93 5752
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2165 3096 3164 3906 3991 5062
Size: 399 222 153 931 68 742 85 1071
Cuts at: 5062 1391
Size: 2174
Fragments arranged by size:
2174 1071 931 742 399 222 153 85
68
BspEI T'CCGG_A
Cuts at: 5508 5508
Size: 5845
BspHI T'CATG_A
Cuts at: 3734 5464 3734
Size: 1730 4115
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2108 2203 2253 2567 3200 844
Size: 1264 95 50 314 633 3489
Fragments arranged by size:
3489 1264 633 314 95 50
BsrBI CCG'CTC
Cuts at: 1394 1823 3732 1394
Size: 429 1909 3507
Fragments arranged by size:
3507 1909 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4340 4514 65
Size: 1183 3092 174 1396
Fragments arranged by size:
3092 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2176 4489 4676 1700
Size: 110 366 2313 187 2869
Fragments arranged by size:
2869 2313 366 187 110
BsrGI T'GTAC_A
Cuts at: 96 2148 2274 2862 96
Size: 2052 126 588 3079
Fragments arranged by size:
3079 2052 588 126
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5845
BssSI C'ACGA_G
Cuts at: 3905 4917 3905
Size: 1012 4833
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2218 2415 794
Size: 1424 197 4224
Fragments arranged by size:
4224 1424 197
BstBI TT'CG_AA
Cuts at: 2118 4649 2118
Size: 2531 3314
BstEII G'GTnAC_C
Cuts at: 1184 2353 3105 1184
Size: 1169 752 3924
Fragments arranged by size:
3924 1169 752
BstXI CCAn_nnnn'nTGG
Cuts at: 2743 2743
Size: 5845
BstYI r'GATC_y
Cuts at: 1972 3175 3542 3927 3944 5396 5482 5494 5840
Size: 1203 367 385 17 1452 86 12 346
Cuts at: 5840 1972
Size: 1977
Fragments arranged by size:
1977 1452 1203 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2069 2433 2895 2937 3054 513
Size: 552 1004 364 462 42 117 3304
Fragments arranged by size:
3304 1004 552 462 364 117 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2632 2722 487
Size: 683 56 1406 90 3610
Fragments arranged by size:
3610 1406 683 90 56
BtsI GCAGTG_nn'
Cuts at: 2061 3361 4155 4175 2061
Size: 1300 794 20 3731
Fragments arranged by size:
3731 1300 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5845
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5810
DraI TTT'AAA
Cuts at: 3149 3149
Size: 5845
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4852 809
Size: 507 3536 1802
Fragments arranged by size:
3536 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2178 2235 2430
Size: 54 1425 50 275 18 348 57 195
Cuts at: 2430 2733 2934 3292 4181 5810 8
Size: 303 201 358 889 1629 43
Fragments arranged by size:
1629 1425 889 358 348 303 275 201
195 57 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3292 1537
Size: 275 1480 4090
Fragments arranged by size:
4090 1480 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2115 2402 3775 1353
Size: 762 287 1373 3423
Fragments arranged by size:
3423 1373 762 287
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2080 2482 4432 4806 4952 387
Size: 1064 138 491 402 1950 374 146 1280
Fragments arranged by size:
1950 1280 1064 491 402 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2468 3067 3908 4505 5292 5560 1239
Size: 393 836 599 841 597 787 268 1524
Fragments arranged by size:
1524 841 836 787 599 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3082 3082
Size: 5845
EcoRI G'AATT_C
Cuts at: 3154 3532 3154
Size: 378 5467
FspI TGC'GCA
Cuts at: 4351 4351
Size: 5845
HaeII r_GCGC'y
Cuts at: 1678 2207 2706 4992 1678
Size: 529 499 2286 2531
Fragments arranged by size:
2531 2286 529 499
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3032 3064 4486 4518 4560
Size: 32 135 32 1460 32 1422 32 42
Cuts at: 4560 4592 1373
Size: 32 2626
Fragments arranged by size:
2626 1460 1422 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3189 3435 669
Size: 988 281 1251 246 3079
Fragments arranged by size:
3079 1251 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5845
HpaI GTT'AAC
Cuts at: 3435 3435
Size: 5845
KpnI G_GTAC'C
Cuts at: 3170 3170
Size: 5845
MfeI C'AATT_G
Cuts at: 3444 3444
Size: 5845
MluI A'CGCG_T
Cuts at: 5721 5721
Size: 5845
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4959 5143 1305
Size: 3654 184 2007
Fragments arranged by size:
3654 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2936 10
Size: 54 1425 343 1104 2919
Fragments arranged by size:
2919 1425 1104 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2051 2741 3803 4162 4321 518
Size: 648 885 690 1062 359 159 2042
Fragments arranged by size:
2042 1062 885 690 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2325 2804 3261 3938 5086 5331 1606
Size: 719 479 457 677 1148 245 2120
Fragments arranged by size:
2120 1148 719 677 479 457 245
NaeI GCC'GGC
Cuts at: 2178 2178
Size: 5845
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5293
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5845
NgoMIV G'CCGG_C
Cuts at: 2176 2176
Size: 5845
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5845
NotI GC'GGCC_GC
Cuts at: 3292 3292
Size: 5845
NspI r_CATG'y
Cuts at: 1132 1229 2572 3203 4748 1132
Size: 97 1343 631 1545 2229
Fragments arranged by size:
2229 1545 1343 631 97
PasI CC'CwG_GG
Cuts at: 1348 2080 1348
Size: 732 5113
PciI A'CATG_T
Cuts at: 4744 4744
Size: 5845
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5845
PmeI GTTT'AAAC
Cuts at: 3149 3149
Size: 5845
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3914 3946 5427 5459 3914
Size: 32 1481 32 4300
Fragments arranged by size:
4300 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3005 1613
Size: 1392 4453
PsiI TTA'TAA
Cuts at: 3415 3415
Size: 5845
PstI C_TGCA'G
Cuts at: 830 2214 2223 2577 3048 3197 830
Size: 1384 9 354 471 149 3478
Fragments arranged by size:
3478 1384 471 354 149 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5845
SalI G'TCGA_C
Cuts at: 1936 3187 1936
Size: 1251 4594
SbfI CC_TGCA'GG
Cuts at: 3197 3197
Size: 5845
ScaI AGT'ACT
Cuts at: 4093 5805 4093
Size: 1712 4133
SexAI A'CCwGG_T
Cuts at: 2781 2781
Size: 5845
SfcI C'TryA_G
Cuts at: 826 1045 2210 2219 2573 2818 3044 3193 4328
Size: 219 1165 9 354 245 226 149 1135
Cuts at: 4328 5009 5200 826
Size: 681 191 1471
Fragments arranged by size:
1471 1165 1135 681 354 245 226 219
191 149 9
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5845
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5845
SmaI CCC'GGG
Cuts at: 1872 3172 1872
Size: 1300 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2698 3949 4850 5112 820
Size: 197 926 755 1251 901 262 1553
Fragments arranged by size:
1553 1251 926 901 755 262 197
SnaBI TAC'GTA
Cuts at: 493 3636 493
Size: 3143 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5845
SphI G_CATG'C
Cuts at: 1132 3203 1132
Size: 2071 3774
SspI AAT'ATT
Cuts at: 5 52 3769 5
Size: 47 3717 2081
Fragments arranged by size:
3717 2081 47
StuI AGG'CCT
Cuts at: 1517 2337 1517
Size: 820 5025
StyI C'CwwG_G
Cuts at: 513 1065 2332 2718 3284 513
Size: 552 1267 386 566 3074
Fragments arranged by size:
3074 1267 566 552 386
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3035 3111 3872 3889 4042 4227 4672
Size: 1255 1455 76 761 17 153 185 445
Cuts at: 4672 325
Size: 1498
Fragments arranged by size:
1498 1455 1255 761 445 185 153 76
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2148 2274 2862
Size: 274 80 33 51 1443 171 126 588
Cuts at: 2862 4091 5803 96
Size: 1229 1712 138
Fragments arranged by size:
1712 1443 1229 588 274 171 138 126
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5805 5837 5805
Size: 32 5813
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5845
XbaI T'CTAG_A
Cuts at: 3181 3181
Size: 5845
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2845 2845
Size: 5845
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5845
XmaI C'CCGG_G
Cuts at: 1870 3170 1870
Size: 1300 4545
XmnI GAAnn'nnTTC
Cuts at: 3974 3974
Size: 5845
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3007 3653 276
Size: 53 83 186 936 1473 646 2468
Fragments arranged by size:
2468 1473 936 646 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoO109I EcoRV FalI FseI FspAI KasI NarI NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SfiI SfoI
SrfI SwaI