Restriction map for
pFN21AE9566
(Circular) (Six-base) MAPSORT of: pFN21AE9566.seq Check: 5168 from: 1 to: 5764
pFN21AE9566 5764 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3574 278
Size: 53 83 186 936 2038 2468
Fragments arranged by size:
2468 2038 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3107 1937
Size: 1170 4594
Acc65I G'GTAC_C
Cuts at: 3085 3085
Size: 5764
AclI AA'CG_TT
Cuts at: 1563 3891 4264 1563
Size: 2328 373 3063
Fragments arranged by size:
3063 2328 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3827 5211 5479 1632
Size: 2195 1384 268 1917
Fragments arranged by size:
2195 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5567
AflIII A'CryG_T
Cuts at: 1203 2388 2634 2901 4663 5640 1203
Size: 1185 246 267 1762 977 1327
Fragments arranged by size:
1762 1327 1185 977 267 246
AgeI A'CCGG_T
Cuts at: 4595 4595
Size: 5764
AhdI GACnn_n'nnGTC
Cuts at: 4493 4493
Size: 5764
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5764
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5764
AlwNI CAG_nnn'CTG
Cuts at: 3507 5079 3507
Size: 1572 4192
ApaLI G'TGCA_C
Cuts at: 3821 4977 3821
Size: 1156 4608
ApoI r'AATT_y
Cuts at: 1493 2183 3073 3299 3451 5560 1493
Size: 690 890 226 152 2109 1697
Fragments arranged by size:
2109 1697 890 690 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4318 160
Size: 872 3286 1606
Fragments arranged by size:
3286 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5764
AvaI C'yCGr_G
Cuts at: 1870 1943 2764 3089 1870
Size: 73 821 325 4545
Fragments arranged by size:
4545 821 325 73
BamHI G'GATC_C
Cuts at: 3094 3461 3094
Size: 367 5397
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2909 3085 4540 618
Size: 325 217 627 1122 176 1455 1842
Fragments arranged by size:
1842 1455 1122 627 325 217 176
BanII G_rGCy'C
Cuts at: 1391 3083 1391
Size: 1692 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2726 928
Size: 971 827 3966
Fragments arranged by size:
3966 971 827
BbvCI CC'TCA_GC
Cuts at: 2987 2987
Size: 5764
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3955 3989 1903
Size: 34 2018 34 3678
Fragments arranged by size:
3678 2018 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3656 4866 3656
Size: 1210 4554
BclI T'GATC_A
Cuts at: 1548 2169 1548
Size: 621 5143
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4375 136
Size: 107 122 71 631 605 2703 1525
Fragments arranged by size:
2703 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5759 5759
Size: 5764
BlpI GC'TnA_GC
Cuts at: 3181 3181
Size: 5764
Bme1580I G_kGCm'C
Cuts at: 1790 3825 4981 1790
Size: 2035 1156 2573
Fragments arranged by size:
2573 2035 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5764
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2273 2507 3495
Size: 416 53 83 399 24 856 234 988
Cuts at: 3495 4453 442
Size: 958 1753
Fragments arranged by size:
1753 988 958 856 416 399 234 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5764
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4424 1239
Size: 3185 2579
Bpu10I CC'TnA_GC
Cuts at: 2987 2987
Size: 5764
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3889 4754 5052 3889
Size: 865 298 4601
Fragments arranged by size:
4601 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4427 882
Size: 617 2928 2219
Fragments arranged by size:
2928 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2635 3555 493
Size: 711 1431 920 2702
Fragments arranged by size:
2702 1431 920 711
BsaBI GATnn'nnATC
Cuts at: 5758 5758
Size: 5764
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2959 3571 3953 4588
Size: 53 83 186 936 1426 612 382 635
Cuts at: 4588 275
Size: 1451
Fragments arranged by size:
1451 1426 936 635 612 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4197 4595 4869 5016 5427 4197
Size: 398 274 147 411 4534
Fragments arranged by size:
4534 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5539
Fragments arranged by size:
5539 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2774 1186
Size: 1588 4176
BseYI C'CCAG_C
Cuts at: 4967 5463 4967
Size: 496 5268
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2837 3060 1163
Size: 573 1101 223 3867
Fragments arranged by size:
3867 1101 573 223
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3214 3975 5003 1540
Size: 275 186 1213 761 1028 2301
Fragments arranged by size:
2301 1213 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3825 3910 4981 3825
Size: 85 1071 4608
Fragments arranged by size:
4608 1071 85
BsmI GAATG_Cn'
Cuts at: 3275 3368 3275
Size: 93 5671
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3083 3825 3910 4981 1391
Size: 399 1293 742 85 1071 2174
Fragments arranged by size:
2174 1293 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5427 5427
Size: 5764
BspHI T'CATG_A
Cuts at: 2397 3653 5383 2397
Size: 1256 1730 2778
Fragments arranged by size:
2778 1730 1256
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2631 3119 844
Size: 1787 488 3489
Fragments arranged by size:
3489 1787 488
BsrBI CCG'CTC
Cuts at: 1394 1823 3651 1394
Size: 429 1828 3507
Fragments arranged by size:
3507 1828 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4259 4433 65
Size: 1183 3011 174 1396
Fragments arranged by size:
3011 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4408 4595 1700
Size: 110 2598 187 2869
Fragments arranged by size:
2869 2598 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5764
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5764
BssSI C'ACGA_G
Cuts at: 3824 4836 3824
Size: 1012 4752
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5764
BstBI TT'CG_AA
Cuts at: 4568 4568
Size: 5764
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5764
BstXI CCAn_nnnn'nTGG
Cuts at: 2365 2479 2593 2365
Size: 114 114 5536
Fragments arranged by size:
5536 114 114
BstYI r'GATC_y
Cuts at: 1972 2628 3094 3461 3846 3863 5315 5401 5413
Size: 656 466 367 385 17 1452 86 12
Cuts at: 5413 5759 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 656 466 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2376 513
Size: 552 1311 3901
Fragments arranged by size:
3901 1311 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2143 2452 487
Size: 683 56 917 309 3799
Fragments arranged by size:
3799 917 683 309 56
BtsI GCAGTG_nn'
Cuts at: 2172 2441 2813 3280 4074 4094 2172
Size: 269 372 467 794 20 3842
Fragments arranged by size:
3842 794 467 372 269 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5764
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5729
DraI TTT'AAA
Cuts at: 3068 3068
Size: 5764
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4771 809
Size: 507 3455 1802
Fragments arranged by size:
3455 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3211 4100 5729
Size: 54 1425 50 275 18 1381 889 1629
Cuts at: 5729 8
Size: 43
Fragments arranged by size:
1629 1425 1381 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3211 1537
Size: 275 1399 4090
Fragments arranged by size:
4090 1399 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2539 3694 1353
Size: 1186 1155 3423
Fragments arranged by size:
3423 1186 1155
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4351 4725 4871 387
Size: 1064 138 2762 374 146 1280
Fragments arranged by size:
2762 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3827 4424 5211 5479 1239
Size: 393 2195 597 787 268 1524
Fragments arranged by size:
2195 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3073 3451 3073
Size: 378 5386
FspI TGC'GCA
Cuts at: 4270 4270
Size: 5764
HaeII r_GCGC'y
Cuts at: 1678 2852 4911 1678
Size: 1174 2059 2531
Fragments arranged by size:
2531 2059 1174
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4405 4437 4479 4511 1373
Size: 32 135 32 2833 32 42 32 2626
Fragments arranged by size:
2833 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2302 3108 3354 669
Size: 988 281 364 806 246 3079
Fragments arranged by size:
3079 988 806 364 281 246
HindIII A'AGCT_T
Cuts at: 748 2090 748
Size: 1342 4422
HpaI GTT'AAC
Cuts at: 3354 3354
Size: 5764
KpnI G_GTAC'C
Cuts at: 3089 3089
Size: 5764
MfeI C'AATT_G
Cuts at: 3363 3363
Size: 5764
MluI A'CGCG_T
Cuts at: 5640 5640
Size: 5764
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2610 4878 5062 1305
Size: 1305 2268 184 2007
Fragments arranged by size:
2268 2007 1305 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3942
Fragments arranged by size:
3942 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2402 2477 2612 2676 3722 4081 4240
Size: 648 1236 75 135 64 1046 359 159
Cuts at: 4240 518
Size: 2042
Fragments arranged by size:
2042 1236 1046 648 359 159 135 75
64
MspA1I CmG'CkG
Cuts at: 1606 2307 3180 3857 5005 5250 1606
Size: 701 873 677 1148 245 2120
Fragments arranged by size:
2120 1148 873 701 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5212
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5764
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5764
NotI GC'GGCC_GC
Cuts at: 3211 3211
Size: 5764
NspI r_CATG'y
Cuts at: 1132 1229 2392 2825 2905 3122 4667 1132
Size: 97 1163 433 80 217 1545 2229
Fragments arranged by size:
2229 1545 1163 433 217 97 80
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5764
PciI A'CATG_T
Cuts at: 2388 2901 4663 2388
Size: 513 1762 3489
Fragments arranged by size:
3489 1762 513
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5764
PmeI GTTT'AAAC
Cuts at: 3068 3068
Size: 5764
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3833 3865 5346 5378 3833
Size: 32 1481 32 4219
Fragments arranged by size:
4219 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5764
PsiI TTA'TAA
Cuts at: 2046 3334 2046
Size: 1288 4476
PstI C_TGCA'G
Cuts at: 830 2804 3116 830
Size: 1974 312 3478
Fragments arranged by size:
3478 1974 312
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5764
SalI G'TCGA_C
Cuts at: 1936 3106 1936
Size: 1170 4594
SapI GCTCTTCn'nnn_
Cuts at: 2539 2539
Size: 5764
SbfI CC_TGCA'GG
Cuts at: 3116 3116
Size: 5764
ScaI AGT'ACT
Cuts at: 4012 5724 4012
Size: 1712 4052
SfcI C'TryA_G
Cuts at: 826 1045 2800 3112 4247 4928 5119 826
Size: 219 1755 312 1135 681 191 1471
Fragments arranged by size:
1755 1471 1135 681 312 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5764
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5764
SmaI CCC'GGG
Cuts at: 1872 2766 3091 1872
Size: 894 325 4545
Fragments arranged by size:
4545 894 325
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3868 4769 5031 820
Size: 197 926 1925 901 262 1553
Fragments arranged by size:
1925 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3555 493
Size: 3062 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5764
SphI G_CATG'C
Cuts at: 1132 3122 1132
Size: 1990 3774
SspI AAT'ATT
Cuts at: 5 52 3688 5
Size: 47 3636 2081
Fragments arranged by size:
3636 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5764
StyI C'CwwG_G
Cuts at: 513 1065 3034 3203 513
Size: 552 1969 169 3074
Fragments arranged by size:
3074 1969 552 169
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2719 3791 3808 3961 4146 4591 325
Size: 1255 1139 1072 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1139 1072 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3012 4010 5722
Size: 274 80 33 51 1443 1035 998 1712
Cuts at: 5722 96
Size: 138
Fragments arranged by size:
1712 1443 1035 998 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5724 5756 5724
Size: 32 5732
Tth111I GACn'n_nGTC
Cuts at: 1316 2581 1316
Size: 1265 4499
XbaI T'CTAG_A
Cuts at: 3100 3100
Size: 5764
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5764
XmaI C'CCGG_G
Cuts at: 1870 2764 3089 1870
Size: 894 325 4545
Fragments arranged by size:
4545 894 325
XmnI GAAnn'nnTTC
Cuts at: 3893 3893
Size: 5764
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3572 276
Size: 53 83 186 936 2038 2468
Fragments arranged by size:
2468 2038 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI