Restriction map for
pFN21AE9563
(Circular) (Six-base) MAPSORT of: pFN21AE9563.seq Check: 8254 from: 1 to: 6142
pFN21AE9563 6142 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2493 3952 278
Size: 53 83 186 936 957 1459 2468
Fragments arranged by size:
2468 1459 957 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3485 1937
Size: 1548 4594
Acc65I G'GTAC_C
Cuts at: 2544 3463 2544
Size: 919 5223
AclI AA'CG_TT
Cuts at: 1563 4269 4642 1563
Size: 2706 373 3063
Fragments arranged by size:
3063 2706 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3281 4205 5589 5857 1632
Size: 1649 924 1384 268 1917
Fragments arranged by size:
1917 1649 1384 924 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5945
AflIII A'CryG_T
Cuts at: 1203 5041 6018 1203
Size: 3838 977 1327
Fragments arranged by size:
3838 1327 977
AgeI A'CCGG_T
Cuts at: 3346 4973 3346
Size: 1627 4515
AhdI GACnn_n'nnGTC
Cuts at: 4871 4871
Size: 6142
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6142
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6142
AlwNI CAG_nnn'CTG
Cuts at: 3885 5457 3885
Size: 1572 4570
ApaLI G'TGCA_C
Cuts at: 4199 5355 4199
Size: 1156 4986
ApoI r'AATT_y
Cuts at: 1493 3451 3677 3829 5938 1493
Size: 1958 226 152 2109 1697
Fragments arranged by size:
2109 1958 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4696 160
Size: 872 3664 1606
Fragments arranged by size:
3664 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6142
AvaI C'yCGr_G
Cuts at: 1870 1943 2841 3467 1870
Size: 73 898 626 4545
Fragments arranged by size:
4545 898 626 73
BamHI G'GATC_C
Cuts at: 3472 3839 3472
Size: 367 5775
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2046 2111 2137 2173 2544
Size: 325 217 627 259 65 26 36 371
Cuts at: 2544 3463 4918 618
Size: 919 1455 1842
Fragments arranged by size:
1842 1455 919 627 371 325 259 217
65 36 26
BanII G_rGCy'C
Cuts at: 1391 2102 2132 2671 3461 1391
Size: 711 30 539 790 4072
Fragments arranged by size:
4072 790 711 539 30
BbeI G_GCGC'C
Cuts at: 2115 2141 2177 2115
Size: 26 36 6080
Fragments arranged by size:
6080 36 26
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2467 928
Size: 971 568 4603
Fragments arranged by size:
4603 971 568
BbvCI CC'TCA_GC
Cuts at: 3299 3299
Size: 6142
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2901 2935 4333 4367 1903
Size: 34 964 34 1398 34 3678
Fragments arranged by size:
3678 1398 964 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4034 5244 4034
Size: 1210 4932
BclI T'GATC_A
Cuts at: 1548 2529 2700 1548
Size: 981 171 4990
Fragments arranged by size:
4990 981 171
BfrBI ATG'CAT
Cuts at: 2598 3250 2598
Size: 652 5490
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2171 4753 136
Size: 107 122 71 631 605 499 2582 1525
Fragments arranged by size:
2582 1525 631 605 499 122 107 71
BglII A'GATC_T
Cuts at: 6137 6137
Size: 6142
BlpI GC'TnA_GC
Cuts at: 3559 3559
Size: 6142
Bme1580I G_kGCm'C
Cuts at: 1790 2049 4203 5359 1790
Size: 259 2154 1156 2573
Fragments arranged by size:
2573 2154 1156 259
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6142
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3873 4831 442
Size: 416 53 83 399 24 2456 958 1753
Fragments arranged by size:
2456 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6142
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2873 3185 3357 4802 1239
Size: 1634 312 172 1445 2579
Fragments arranged by size:
2579 1634 1445 312 172
Bpu10I CC'TnA_GC
Cuts at: 3299 3299
Size: 6142
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2116 2915 4267 5132 5430 2116
Size: 799 1352 865 298 2828
Fragments arranged by size:
2828 1352 865 799 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3277 3322 4805 882
Size: 617 1778 45 1483 2219
Fragments arranged by size:
2219 1778 1483 617 45
BsaAI yAC'GTr
Cuts at: 493 1204 3933 493
Size: 711 2729 2702
Fragments arranged by size:
2729 2702 711
BsaBI GATnn'nnATC
Cuts at: 6136 6136
Size: 6142
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2112 2138 2174 2490
Size: 53 83 186 936 579 26 36 316
Cuts at: 2490 3949 4331 4966 275
Size: 1459 382 635 1451
Fragments arranged by size:
1459 1451 936 635 579 382 316 186
83 53 36 26
BsaWI w'CCGG_w
Cuts at: 3213 3225 3346 4575 4973 5247 5394 5805 3213
Size: 12 121 1229 398 274 147 411 3550
Fragments arranged by size:
3550 1229 411 398 274 147 121 12
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2189 2219 2635 2665 1178
Size: 30 165 30 786 30 416 30 4655
Fragments arranged by size:
4655 786 416 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2918 3029 1186
Size: 1732 111 4299
Fragments arranged by size:
4299 1732 111
BseYI C'CCAG_C
Cuts at: 5345 5841 5345
Size: 496 5646
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2485 3292 1163
Size: 573 749 807 4013
Fragments arranged by size:
4013 807 749 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2029 2124 2292 3592 4353 5381
Size: 275 186 28 95 168 1300 761 1028
Cuts at: 5381 1540
Size: 2301
Fragments arranged by size:
2301 1300 1028 761 275 186 168 95
28
BsiHKAI G_wGCw'C
Cuts at: 4203 4288 5359 4203
Size: 85 1071 4986
Fragments arranged by size:
4986 1071 85
BsmI GAATG_Cn'
Cuts at: 3653 3746 3653
Size: 93 6049
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2049 2102 2132 2671 3461 4203 4288
Size: 399 259 53 30 539 790 742 85
Cuts at: 4288 5359 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 790 742 539 399 259 85
53 30
BspEI T'CCGG_A
Cuts at: 5805 5805
Size: 6142
BspHI T'CATG_A
Cuts at: 2514 3429 3436 4031 5761 2514
Size: 915 7 595 1730 2895
Fragments arranged by size:
2895 1730 915 595 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3497 844
Size: 2653 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2055 4029 1394
Size: 429 232 1974 3507
Fragments arranged by size:
3507 1974 429 232
BsrDI GCAATG_nn'
Cuts at: 65 1248 4637 4811 65
Size: 1183 3389 174 1396
Fragments arranged by size:
3389 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3346 4786 4973 1700
Size: 110 1536 1440 187 2869
Fragments arranged by size:
2869 1536 1440 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6142
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6142
BssSI C'ACGA_G
Cuts at: 4202 5214 4202
Size: 1012 5130
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3075 794
Size: 2281 3861
BstBI TT'CG_AA
Cuts at: 4946 4946
Size: 6142
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6142
BstXI CCAn_nnnn'nTGG
Cuts at: 2714 2989 2714
Size: 275 5867
BstYI r'GATC_y
Cuts at: 1972 2448 3472 3839 4224 4241 5693 5779 5791
Size: 476 1024 367 385 17 1452 86 12
Cuts at: 5791 6137 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1024 476 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3176 3176
Size: 6142
BtgI C'CryG_G
Cuts at: 513 1065 2484 513
Size: 552 1419 4171
Fragments arranged by size:
4171 1419 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2464 2848 487
Size: 683 56 1238 384 3781
Fragments arranged by size:
3781 1238 683 384 56
BtsI GCAGTG_nn'
Cuts at: 2745 3658 4452 4472 2745
Size: 913 794 20 4415
Fragments arranged by size:
4415 913 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6142
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6107
DraI TTT'AAA
Cuts at: 3446 3446
Size: 6142
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5149 809
Size: 507 3833 1802
Fragments arranged by size:
3833 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2026 2379 3589
Size: 54 1425 50 275 18 196 353 1210
Cuts at: 3589 4478 6107 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1210 889 353 275 196 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2026 3589 1537
Size: 275 214 1563 4090
Fragments arranged by size:
4090 1563 275 214
EarI CTCTTCn'nnn_
Cuts at: 1353 2801 2898 4072 1353
Size: 1448 97 1174 3423
Fragments arranged by size:
3423 1448 1174 97
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2330 2623 4729 5103 5249 387
Size: 1064 138 741 293 2106 374 146 1280
Fragments arranged by size:
2106 1280 1064 741 374 293 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2873 3185 3281 3357 4205 4802 5589
Size: 393 1241 312 96 76 848 597 787
Cuts at: 5589 5857 1239
Size: 268 1524
Fragments arranged by size:
1524 1241 848 787 597 393 312 268
96 76
EcoNI CCTnn'n_nnAGG
Cuts at: 3310 3310
Size: 6142
EcoO109I rG'GnC_Cy
Cuts at: 2562 3006 3172 3303 2562
Size: 444 166 131 5401
Fragments arranged by size:
5401 444 166 131
EcoRI G'AATT_C
Cuts at: 3451 3829 3451
Size: 378 5764
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2467 2713 2467
Size: 246 5896
FspI TGC'GCA
Cuts at: 4648 4648
Size: 6142
HaeII r_GCGC'y
Cuts at: 1678 2115 2141 2177 2378 5289 1678
Size: 437 26 36 201 2911 2531
Fragments arranged by size:
2911 2531 437 201 36 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2189 2221 3045 3077 3172
Size: 32 135 32 617 32 824 32 95
Cuts at: 3172 3204 4783 4815 4857 4889 1373
Size: 32 1579 32 42 32 2626
Fragments arranged by size:
2626 1579 824 617 135 95 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2017 3486 3732 669
Size: 988 281 79 1469 246 3079
Fragments arranged by size:
3079 1469 988 281 246 79
HindIII A'AGCT_T
Cuts at: 748 3263 748
Size: 2515 3627
HpaI GTT'AAC
Cuts at: 2017 3732 2017
Size: 1715 4427
KasI G'GCGC_C
Cuts at: 2111 2137 2173 2111
Size: 26 36 6080
Fragments arranged by size:
6080 36 26
KpnI G_GTAC'C
Cuts at: 2548 3467 2548
Size: 919 5223
MfeI C'AATT_G
Cuts at: 3385 3741 3385
Size: 356 5786
MluI A'CGCG_T
Cuts at: 6018 6018
Size: 6142
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2376 5256 5440 1305
Size: 1071 2880 184 2007
Fragments arranged by size:
2880 2007 1071 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4320
Fragments arranged by size:
4320 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2483 3112 4100 4459 4618 518
Size: 648 1317 629 988 359 159 2042
Fragments arranged by size:
2042 1317 988 648 629 359 159
MspA1I CmG'CkG
Cuts at: 1606 2025 3081 3239 3558 4235 5383 5628 1606
Size: 419 1056 158 319 677 1148 245 2120
Fragments arranged by size:
2120 1148 1056 677 419 319 245 158
NarI GG'CG_CC
Cuts at: 2112 2138 2174 2112
Size: 26 36 6080
Fragments arranged by size:
6080 36 26
NcoI C'CATG_G
Cuts at: 513 1065 2484 513
Size: 552 1419 4171
Fragments arranged by size:
4171 1419 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6142
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6142
NotI GC'GGCC_GC
Cuts at: 2026 3589 2026
Size: 1563 4579
NsiI A_TGCA'T
Cuts at: 2600 3252 2600
Size: 652 5490
NspI r_CATG'y
Cuts at: 1132 1229 3250 3500 5045 1132
Size: 97 2021 250 1545 2229
Fragments arranged by size:
2229 2021 1545 250 97
PasI CC'CwG_GG
Cuts at: 1348 2989 1348
Size: 1641 4501
PciI A'CATG_T
Cuts at: 5041 5041
Size: 6142
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2660 1108
Size: 1552 4590
PmeI GTTT'AAAC
Cuts at: 3446 3446
Size: 6142
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4211 4243 5724 5756 4211
Size: 32 1481 32 4597
Fragments arranged by size:
4597 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3172 3172
Size: 6142
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6142
PsiI TTA'TAA
Cuts at: 3712 3712
Size: 6142
PspXI vC'TCGA_Gb
Cuts at: 2841 2841
Size: 6142
PstI C_TGCA'G
Cuts at: 830 3080 3244 3494 830
Size: 2250 164 250 3478
Fragments arranged by size:
3478 2250 250 164
PvuI CG_AT'CG
Cuts at: 2001 2292 2001
Size: 291 5851
PvuII CAG'CTG
Cuts at: 3081 3239 3081
Size: 158 5984
SalI G'TCGA_C
Cuts at: 1936 3484 1936
Size: 1548 4594
SbfI CC_TGCA'GG
Cuts at: 3494 3494
Size: 6142
ScaI AGT'ACT
Cuts at: 4390 6102 4390
Size: 1712 4430
SfcI C'TryA_G
Cuts at: 826 1045 3076 3240 3414 3490 4625 5306 5497
Size: 219 2031 164 174 76 1135 681 191
Cuts at: 5497 826
Size: 1471
Fragments arranged by size:
2031 1471 1135 681 219 191 174 164
76
SfoI GGC'GCC
Cuts at: 2113 2139 2175 2113
Size: 26 36 6080
Fragments arranged by size:
6080 36 26
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6142
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6142
SmaI CCC'GGG
Cuts at: 1872 3469 1872
Size: 1597 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2095 2841 2930 4246 5147 5409
Size: 197 926 152 746 89 1316 901 262
Cuts at: 5409 820
Size: 1553
Fragments arranged by size:
1553 1316 926 901 746 262 197 152
89
SnaBI TAC'GTA
Cuts at: 493 3933 493
Size: 3440 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6142
SphI G_CATG'C
Cuts at: 1132 3250 3500 1132
Size: 2118 250 3774
Fragments arranged by size:
3774 2118 250
SspI AAT'ATT
Cuts at: 5 52 4066 5
Size: 47 4014 2081
Fragments arranged by size:
4014 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6142
StyI C'CwwG_G
Cuts at: 513 1065 2484 2548 3581 513
Size: 552 1419 64 1033 3074
Fragments arranged by size:
3074 1419 1033 552 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3085 3349 4169 4186 4339 4524 4969
Size: 1255 1505 264 820 17 153 185 445
Cuts at: 4969 325
Size: 1498
Fragments arranged by size:
1505 1498 1255 820 445 264 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2778 4388 6100
Size: 274 80 33 51 1443 801 1610 1712
Cuts at: 6100 96
Size: 138
Fragments arranged by size:
1712 1610 1443 801 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2480 2512 2635 2667 6102 6134 2480
Size: 32 123 32 3435 32 2488
Fragments arranged by size:
3435 2488 123 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2120 2858 1316
Size: 804 738 4600
Fragments arranged by size:
4600 804 738
XbaI T'CTAG_A
Cuts at: 3478 3478
Size: 6142
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2661 2661
Size: 6142
XhoI C'TCGA_G
Cuts at: 1943 2841 1943
Size: 898 5244
XmaI C'CCGG_G
Cuts at: 1870 3467 1870
Size: 1597 4545
XmnI GAAnn'nnTTC
Cuts at: 4271 4271
Size: 6142
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2491 3950 276
Size: 53 83 186 936 957 1459 2468
Fragments arranged by size:
2468 1459 957 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI
PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BplI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI
NaeI NgoMIV NruI PacI PfoI PmlI PspOMI PsrI
RsrII SacI SacII SanDI SapI SexAI SfiI SrfI
SwaI