Restriction map for
pFN21AE9562
(Circular) (Six-base) MAPSORT of: pFN21AE9562.seq Check: 9229 from: 1 to: 5791
pFN21AE9562 5791 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2169 3601 278
Size: 53 83 186 936 633 1432 2468
Fragments arranged by size:
2468 1432 936 633 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3134 1937
Size: 1197 4594
Acc65I G'GTAC_C
Cuts at: 3112 3112
Size: 5791
AclI AA'CG_TT
Cuts at: 1563 3918 4291 1563
Size: 2355 373 3063
Fragments arranged by size:
3063 2355 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2672 2750 3854 5238 5506 1632
Size: 1040 78 1104 1384 268 1917
Fragments arranged by size:
1917 1384 1104 1040 268 78
AfeI AGC'GCT
Cuts at: 2130 2130
Size: 5791
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5594
AflIII A'CryG_T
Cuts at: 1203 4690 5667 1203
Size: 3487 977 1327
Fragments arranged by size:
3487 1327 977
AgeI A'CCGG_T
Cuts at: 4622 4622
Size: 5791
AhdI GACnn_n'nnGTC
Cuts at: 4520 4520
Size: 5791
AleI CACnn'nnGTG
Cuts at: 1166 2159 2510 1166
Size: 993 351 4447
Fragments arranged by size:
4447 993 351
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2693 1184
Size: 1509 4282
AlwNI CAG_nnn'CTG
Cuts at: 3534 5106 3534
Size: 1572 4219
ApaI G_GGCC'C
Cuts at: 2326 2326
Size: 5791
ApaLI G'TGCA_C
Cuts at: 3848 5004 3848
Size: 1156 4635
ApoI r'AATT_y
Cuts at: 1493 3100 3326 3478 5587 1493
Size: 1607 226 152 2109 1697
Fragments arranged by size:
2109 1697 1607 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4345 160
Size: 872 3313 1606
Fragments arranged by size:
3313 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5791
AvaI C'yCGr_G
Cuts at: 1870 1943 2062 3116 1870
Size: 73 119 1054 4545
Fragments arranged by size:
4545 1054 119 73
BamHI G'GATC_C
Cuts at: 2967 3121 3488 2967
Size: 154 367 5270
Fragments arranged by size:
5270 367 154
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3112 4567 618
Size: 325 217 627 1325 1455 1842
Fragments arranged by size:
1842 1455 1325 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2326 2800 2934 3047 3110 1391
Size: 935 474 134 113 63 4072
Fragments arranged by size:
4072 935 474 134 113 63
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2380 928
Size: 971 481 4339
Fragments arranged by size:
4339 971 481
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2800 2834 3982 4016 1903
Size: 34 863 34 1148 34 3678
Fragments arranged by size:
3678 1148 863 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3683 4893 3683
Size: 1210 4581
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5791
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4402 136
Size: 107 122 71 631 605 2730 1525
Fragments arranged by size:
2730 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5786 5786
Size: 5791
BlpI GC'TnA_GC
Cuts at: 3208 3208
Size: 5791
Bme1580I G_kGCm'C
Cuts at: 1790 2326 3852 5008 1790
Size: 536 1526 1156 2573
Fragments arranged by size:
2573 1526 1156 536
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5791
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2593 3522 4480
Size: 416 53 83 399 24 1176 929 958
Cuts at: 4480 442
Size: 1753
Fragments arranged by size:
1753 1176 958 929 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5791
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2035 3066 2035
Size: 1031 4760
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2080 2109 2567 4451 1239
Size: 841 29 458 1884 2579
Fragments arranged by size:
2579 1884 841 458 29
Bpu10I CC'TnA_GC
Cuts at: 2201 2369 2201
Size: 168 5623
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3916 4781 5079 3916
Size: 865 298 4628
Fragments arranged by size:
4628 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4454 882
Size: 617 2955 2219
Fragments arranged by size:
2955 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3582 493
Size: 711 2378 2702
Fragments arranged by size:
2702 2378 711
BsaBI GATnn'nnATC
Cuts at: 5785 5785
Size: 5791
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2166 3598 3980 4615
Size: 53 83 186 936 633 1432 382 635
Cuts at: 4615 275
Size: 1451
Fragments arranged by size:
1451 1432 936 635 633 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2217 4224 4622 4896 5043 5454 2217
Size: 2007 398 274 147 411 2554
Fragments arranged by size:
2554 2007 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5566
Fragments arranged by size:
5566 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2348 2492 2624 1186
Size: 1162 144 132 4353
Fragments arranged by size:
4353 1162 144 132
BseYI C'CCAG_C
Cuts at: 2319 2735 2793 3046 4994 5490 2319
Size: 416 58 253 1948 496 2620
Fragments arranged by size:
2620 1948 496 416 253 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2465 2557 3089 1163
Size: 573 729 92 532 3865
Fragments arranged by size:
3865 729 573 532 92
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3241 4002 5030 1540
Size: 275 186 1240 761 1028 2301
Fragments arranged by size:
2301 1240 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2145 2202 2340 3852 3937 5008 2145
Size: 57 138 1512 85 1071 2928
Fragments arranged by size:
2928 1512 1071 138 85 57
BsmI GAATG_Cn'
Cuts at: 2920 3000 3302 3395 2920
Size: 80 302 93 5316
Fragments arranged by size:
5316 302 93 80
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2145 2202 2326 2340 2800 2934 3047
Size: 399 355 57 124 14 460 134 113
Cuts at: 3047 3110 3852 3937 5008 1391
Size: 63 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 460 399 355 134 124
113 85 63 57 14
BspEI T'CCGG_A
Cuts at: 2217 5454 2217
Size: 3237 2554
BspHI T'CATG_A
Cuts at: 3680 5410 3680
Size: 1730 4061
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2216 2673 3146 844
Size: 1372 457 473 3489
Fragments arranged by size:
3489 1372 473 457
BsrBI CCG'CTC
Cuts at: 1394 1823 3678 1394
Size: 429 1855 3507
Fragments arranged by size:
3507 1855 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4286 4460 65
Size: 1183 3038 174 1396
Fragments arranged by size:
3038 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2460 4435 4622 1700
Size: 110 650 1975 187 2869
Fragments arranged by size:
2869 1975 650 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5791
BssHII G'CGCG_C
Cuts at: 1925 2085 1925
Size: 160 5631
BssSI C'ACGA_G
Cuts at: 2297 3851 4863 2297
Size: 1554 1012 3225
Fragments arranged by size:
3225 1554 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2724 794
Size: 1930 3861
BstBI TT'CG_AA
Cuts at: 4595 4595
Size: 5791
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5791
BstXI CCAn_nnnn'nTGG
Cuts at: 2161 2767 2161
Size: 606 5185
BstYI r'GATC_y
Cuts at: 1972 2856 2967 3121 3488 3873 3890 5342 5428
Size: 884 111 154 367 385 17 1452 86
Cuts at: 5428 5440 5786 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 884 385 367 346 154 111
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2862 2862
Size: 5791
BtgI C'CryG_G
Cuts at: 513 1065 2136 2160 2508 2990 513
Size: 552 1071 24 348 482 3314
Fragments arranged by size:
3314 1071 552 482 348 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2389 487
Size: 683 56 1163 3889
Fragments arranged by size:
3889 1163 683 56
BtsI GCAGTG_nn'
Cuts at: 2061 2338 2397 2619 2995 3307 4101 4121 2061
Size: 277 59 222 376 312 794 20 3731
Fragments arranged by size:
3731 794 376 312 277 222 59 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5791
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5756
DraI TTT'AAA
Cuts at: 3095 3095
Size: 5791
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4798 809
Size: 507 3482 1802
Fragments arranged by size:
3482 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2301 2826 3238
Size: 54 1425 50 275 18 471 525 412
Cuts at: 3238 4127 5756 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 525 471 412 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3238 1537
Size: 275 1426 4090
Fragments arranged by size:
4090 1426 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3721 1353
Size: 2368 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4378 4752 4898 387
Size: 1064 138 2789 374 146 1280
Fragments arranged by size:
2789 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2080 2109 2567 2672 2750 3854 4451
Size: 393 448 29 458 105 78 1104 597
Cuts at: 4451 5238 5506 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1104 787 597 458 448 393 268
105 78 29
EcoNI CCTnn'n_nnAGG
Cuts at: 2330 2330
Size: 5791
EcoO109I rG'GnC_Cy
Cuts at: 2100 2323 2541 2100
Size: 223 218 5350
Fragments arranged by size:
5350 223 218
EcoRI G'AATT_C
Cuts at: 3100 3478 3100
Size: 378 5413
FspI TGC'GCA
Cuts at: 2669 4297 2669
Size: 1628 4163
HaeII r_GCGC'y
Cuts at: 1678 2132 2355 2467 4938 1678
Size: 454 223 112 2471 2531
Fragments arranged by size:
2531 2471 454 223 112
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4432 4464 4506 4538 1373
Size: 32 135 32 2860 32 42 32 2626
Fragments arranged by size:
2860 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3135 3381 669
Size: 988 281 1197 246 3079
Fragments arranged by size:
3079 1197 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5791
HpaI GTT'AAC
Cuts at: 3381 3381
Size: 5791
KpnI G_GTAC'C
Cuts at: 3116 3116
Size: 5791
MfeI C'AATT_G
Cuts at: 3390 3390
Size: 5791
MluI A'CGCG_T
Cuts at: 5667 5667
Size: 5791
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4905 5089 1305
Size: 3600 184 2007
Fragments arranged by size:
3600 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2303 2828 10
Size: 54 1425 343 471 525 2973
Fragments arranged by size:
2973 1425 525 471 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2159 2510 2675 2765 3065 3749 4108
Size: 648 993 351 165 90 300 684 359
Cuts at: 4108 4267 518
Size: 159 2042
Fragments arranged by size:
2042 993 684 648 359 351 300 165
159 90
MspA1I CmG'CkG
Cuts at: 1606 2138 2907 3207 3884 5032 5277 1606
Size: 532 769 300 677 1148 245 2120
Fragments arranged by size:
2120 1148 769 677 532 300 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5239
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5791
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5791
NotI GC'GGCC_GC
Cuts at: 3238 3238
Size: 5791
NspI r_CATG'y
Cuts at: 1132 1229 3149 4694 1132
Size: 97 1920 1545 2229
Fragments arranged by size:
2229 1920 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2115 2326 1348
Size: 767 211 4813
Fragments arranged by size:
4813 767 211
PciI A'CATG_T
Cuts at: 4690 4690
Size: 5791
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2440 2793 2845 1108
Size: 1332 353 52 4054
Fragments arranged by size:
4054 1332 353 52
PfoI T'CCnGG_A
Cuts at: 2096 2096
Size: 5791
PmeI GTTT'AAAC
Cuts at: 3095 3095
Size: 5791
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3860 3892 5373 5405 3860
Size: 32 1481 32 4246
Fragments arranged by size:
4246 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2100 2541 2100
Size: 441 5350
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5791
PsiI TTA'TAA
Cuts at: 3361 3361
Size: 5791
PspOMI G'GGCC_C
Cuts at: 2322 2322
Size: 5791
PstI C_TGCA'G
Cuts at: 830 3042 3143 830
Size: 2212 101 3478
Fragments arranged by size:
3478 2212 101
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5791
PvuII CAG'CTG
Cuts at: 2907 2907
Size: 5791
SacII CC_GC'GG
Cuts at: 2139 2139
Size: 5791
SalI G'TCGA_C
Cuts at: 1936 3133 1936
Size: 1197 4594
SanDI GG'GwC_CC
Cuts at: 2541 2541
Size: 5791
SbfI CC_TGCA'GG
Cuts at: 3143 3143
Size: 5791
ScaI AGT'ACT
Cuts at: 4039 5751 4039
Size: 1712 4079
SexAI A'CCwGG_T
Cuts at: 2262 2262
Size: 5791
SfcI C'TryA_G
Cuts at: 826 1045 3038 3139 4274 4955 5146 826
Size: 219 1993 101 1135 681 191 1471
Fragments arranged by size:
1993 1471 1135 681 219 191 101
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5791
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5791
SmaI CCC'GGG
Cuts at: 1872 2064 3118 1872
Size: 192 1054 4545
Fragments arranged by size:
4545 1054 192
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3895 4796 5058 820
Size: 197 926 1952 901 262 1553
Fragments arranged by size:
1952 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3582 493
Size: 3089 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5791
SphI G_CATG'C
Cuts at: 1132 3149 1132
Size: 2017 3774
SrfI GCCC'GGGC
Cuts at: 2064 2064
Size: 5791
SspI AAT'ATT
Cuts at: 5 52 3715 5
Size: 47 3663 2081
Fragments arranged by size:
3663 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5791
StyI C'CwwG_G
Cuts at: 513 1065 3015 3230 513
Size: 552 1950 215 3074
Fragments arranged by size:
3074 1950 552 215
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2107 2612 2665 3070 3818 3835 3988
Size: 1255 527 505 53 405 748 17 153
Cuts at: 3988 4173 4618 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 748 527 505 445 405 185
153 53 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2601 4037 5749
Size: 274 80 33 51 1443 624 1436 1712
Cuts at: 5749 96
Size: 138
Fragments arranged by size:
1712 1443 1436 624 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5751 5783 5751
Size: 32 5759
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5791
XbaI T'CTAG_A
Cuts at: 3127 3127
Size: 5791
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2533 2533
Size: 5791
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5791
XmaI C'CCGG_G
Cuts at: 1870 2062 3116 1870
Size: 192 1054 4545
Fragments arranged by size:
4545 1054 192
XmnI GAAnn'nnTTC
Cuts at: 3920 3920
Size: 5791
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2167 3599 276
Size: 53 83 186 936 633 1432 2468
Fragments arranged by size:
2468 1432 936 633 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII
SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspXI PsrI RsrII SacI SapI SfiI SfoI
SwaI