Restriction map for
pFN21AE9559
(Circular) (Six-base) MAPSORT of: pFN21AE9559.seq Check: 6705 from: 1 to: 5758
pFN21AE9559 5758 bp
With 182 enzymes: SgfI *
August 25, 2011 15:07 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3568 278
Size: 53 83 186 936 2032 2468
Fragments arranged by size:
2468 2032 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3101 1937
Size: 1164 4594
Acc65I G'GTAC_C
Cuts at: 3079 3079
Size: 5758
AclI AA'CG_TT
Cuts at: 1563 3885 4258 1563
Size: 2322 373 3063
Fragments arranged by size:
3063 2322 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3821 5205 5473 1632
Size: 2189 1384 268 1917
Fragments arranged by size:
2189 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5561
AflIII A'CryG_T
Cuts at: 1203 4657 5634 1203
Size: 3454 977 1327
Fragments arranged by size:
3454 1327 977
AgeI A'CCGG_T
Cuts at: 4589 4589
Size: 5758
AhdI GACnn_n'nnGTC
Cuts at: 4487 4487
Size: 5758
AleI CACnn'nnGTG
Cuts at: 1166 2397 1166
Size: 1231 4527
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5758
AlwNI CAG_nnn'CTG
Cuts at: 3501 5073 3501
Size: 1572 4186
ApaLI G'TGCA_C
Cuts at: 3815 4971 3815
Size: 1156 4602
ApoI r'AATT_y
Cuts at: 1493 3067 3293 3445 5554 1493
Size: 1574 226 152 2109 1697
Fragments arranged by size:
2109 1697 1574 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4312 160
Size: 872 3280 1606
Fragments arranged by size:
3280 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5758
AvaI C'yCGr_G
Cuts at: 1870 1943 3023 3083 1870
Size: 73 1080 60 4545
Fragments arranged by size:
4545 1080 73 60
BamHI G'GATC_C
Cuts at: 3088 3455 3088
Size: 367 5391
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2391 2548 2999 3079 4534
Size: 325 217 627 604 157 451 80 1455
Cuts at: 4534 618
Size: 1842
Fragments arranged by size:
1842 1455 627 604 451 325 217 157
80
BanII G_rGCy'C
Cuts at: 1391 2076 2587 3077 1391
Size: 685 511 490 4072
Fragments arranged by size:
4072 685 511 490
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2596 928
Size: 971 697 4090
Fragments arranged by size:
4090 971 697
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3949 3983 1903
Size: 34 2012 34 3678
Fragments arranged by size:
3678 2012 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3650 4860 3650
Size: 1210 4548
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5758
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2582 4369 136
Size: 107 122 71 631 605 910 1787 1525
Fragments arranged by size:
1787 1525 910 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5753 5753
Size: 5758
BlpI GC'TnA_GC
Cuts at: 2075 2558 2714 3175 2075
Size: 483 156 461 4658
Fragments arranged by size:
4658 483 461 156
Bme1580I G_kGCm'C
Cuts at: 1790 2527 3819 4975 1790
Size: 737 1292 1156 2573
Fragments arranged by size:
2573 1292 1156 737
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5758
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2126 3489 4447
Size: 416 53 83 399 24 709 1363 958
Cuts at: 4447 442
Size: 1753
Fragments arranged by size:
1753 1363 958 709 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5758
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2095 2881 2095
Size: 786 4972
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4418 1239
Size: 3179 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2327 3883 4748 5046 2327
Size: 1556 865 298 3039
Fragments arranged by size:
3039 1556 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2030 2699 4421 882
Size: 617 531 669 1722 2219
Fragments arranged by size:
2219 1722 669 617 531
BsaAI yAC'GTr
Cuts at: 493 1204 2647 3549 493
Size: 711 1443 902 2702
Fragments arranged by size:
2702 1443 902 711
BsaBI GATnn'nnATC
Cuts at: 5752 5752
Size: 5758
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3565 3947 4582 275
Size: 53 83 186 936 2032 382 635 1451
Fragments arranged by size:
2032 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4191 4589 4863 5010 5421 4191
Size: 398 274 147 411 4528
Fragments arranged by size:
4528 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5533
Fragments arranged by size:
5533 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5758
BseYI C'CCAG_C
Cuts at: 4961 5457 4961
Size: 496 5262
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2402 2432 2681 1163
Size: 573 666 30 249 4240
Fragments arranged by size:
4240 666 573 249 30
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3208 3969 4997 1540
Size: 275 186 1207 761 1028 2301
Fragments arranged by size:
2301 1207 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2076 3819 3904 4975 2076
Size: 1743 85 1071 2859
Fragments arranged by size:
2859 1743 1071 85
BsmI GAATG_Cn'
Cuts at: 3269 3362 3269
Size: 93 5665
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2076 2527 2587 3077 3819 3904 4975
Size: 399 286 451 60 490 742 85 1071
Cuts at: 4975 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 490 451 399 286 85
60
BspEI T'CCGG_A
Cuts at: 5421 5421
Size: 5758
BspHI T'CATG_A
Cuts at: 3647 5377 3647
Size: 1730 4028
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2220 3113 844
Size: 1376 893 3489
Fragments arranged by size:
3489 1376 893
BsrBI CCG'CTC
Cuts at: 1394 1823 2389 2565 3645 1394
Size: 429 566 176 1080 3507
Fragments arranged by size:
3507 1080 566 429 176
BsrDI GCAATG_nn'
Cuts at: 65 1248 2824 4253 4427 65
Size: 1183 1576 1429 174 1396
Fragments arranged by size:
1576 1429 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4402 4589 1700
Size: 110 2592 187 2869
Fragments arranged by size:
2869 2592 187 110
BsrGI T'GTAC_A
Cuts at: 96 2625 96
Size: 2529 3229
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5758
BssSI C'ACGA_G
Cuts at: 3818 4830 3818
Size: 1012 4746
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2332 794
Size: 1538 4220
BstBI TT'CG_AA
Cuts at: 4562 4562
Size: 5758
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5758
BstXI CCAn_nnnn'nTGG
Cuts at: 2101 2158 2371 2383 2869 2101
Size: 57 213 12 486 4990
Fragments arranged by size:
4990 486 213 57 12
BstYI r'GATC_y
Cuts at: 1972 3088 3455 3840 3857 5309 5395 5407 5753
Size: 1116 367 385 17 1452 86 12 346
Cuts at: 5753 1972
Size: 1977
Fragments arranged by size:
1977 1452 1116 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2229 2382 2434 513
Size: 552 939 225 153 52 3837
Fragments arranged by size:
3837 939 552 225 153 52
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2158 2362 487
Size: 683 56 932 204 3883
Fragments arranged by size:
3883 932 683 204 56
BtsI GCAGTG_nn'
Cuts at: 2349 2366 2404 2512 3274 4068 4088 2349
Size: 17 38 108 762 794 20 4019
Fragments arranged by size:
4019 794 762 108 38 20 17
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5758
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5723
DraI TTT'AAA
Cuts at: 3062 3062
Size: 5758
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4765 809
Size: 507 3449 1802
Fragments arranged by size:
3449 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2226 2361 2373
Size: 54 1425 50 275 18 396 135 12
Cuts at: 2373 2406 2437 2574 2853 3031 3205 4094 5723
Size: 33 31 137 279 178 174 889 1629
Cuts at: 5723 8
Size: 43
Fragments arranged by size:
1629 1425 889 396 279 275 178 174
137 135 54 50 43 33 31 18
12
EagI C'GGCC_G
Cuts at: 1537 1812 3205 1537
Size: 275 1393 4090
Fragments arranged by size:
4090 1393 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2246 2267 3688 1353
Size: 893 21 1421 3423
Fragments arranged by size:
3423 1421 893 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2912 2939 4345 4719 4865 387
Size: 1064 138 1323 27 1406 374 146 1280
Fragments arranged by size:
1406 1323 1280 1064 374 146 138 27
EcoICRI GAG'CTC
Cuts at: 2074 2074
Size: 5758
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3821 4418 5205 5473 1239
Size: 393 2189 597 787 268 1524
Fragments arranged by size:
2189 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2324 2324
Size: 5758
EcoRI G'AATT_C
Cuts at: 3067 3445 3067
Size: 378 5380
FspI TGC'GCA
Cuts at: 4264 4264
Size: 5758
HaeII r_GCGC'y
Cuts at: 1678 4905 1678
Size: 3227 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2522 2554 4399 4431 4473
Size: 32 135 32 950 32 1845 32 42
Cuts at: 4473 4505 1373
Size: 32 2626
Fragments arranged by size:
2626 1845 950 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3102 3348 669
Size: 988 281 1164 246 3079
Fragments arranged by size:
3079 1164 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5758
HpaI GTT'AAC
Cuts at: 3348 3348
Size: 5758
KpnI G_GTAC'C
Cuts at: 3083 3083
Size: 5758
MfeI C'AATT_G
Cuts at: 3357 3357
Size: 5758
MluI A'CGCG_T
Cuts at: 5634 5634
Size: 5758
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2205 4872 5056 1305
Size: 900 2667 184 2007
Fragments arranged by size:
2667 2007 900 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2363 2375 10
Size: 54 1425 343 531 12 3393
Fragments arranged by size:
3393 1425 531 343 54 12
MslI CAynn'nnrTG
Cuts at: 518 1166 2381 2397 3716 4075 4234 518
Size: 648 1215 16 1319 359 159 2042
Fragments arranged by size:
2042 1319 1215 648 359 159 16
MspA1I CmG'CkG
Cuts at: 1606 2231 2416 2535 2685 3174 3851 4999 5244
Size: 625 185 119 150 489 677 1148 245
Cuts at: 5244 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 625 489 245 185 150
119
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4267
Fragments arranged by size:
4267 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5758
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5758
NotI GC'GGCC_GC
Cuts at: 3205 3205
Size: 5758
NspI r_CATG'y
Cuts at: 1132 1229 2681 3116 4661 1132
Size: 97 1452 435 1545 2229
Fragments arranged by size:
2229 1545 1452 435 97
PasI CC'CwG_GG
Cuts at: 1348 2479 2992 1348
Size: 1131 513 4114
Fragments arranged by size:
4114 1131 513
PciI A'CATG_T
Cuts at: 4657 4657
Size: 5758
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5758
PfoI T'CCnGG_A
Cuts at: 2790 2790
Size: 5758
PmeI GTTT'AAAC
Cuts at: 3062 3062
Size: 5758
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2300 2332 3827 3859 5340 5372 2300
Size: 32 1495 32 1481 32 2686
Fragments arranged by size:
2686 1495 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2732 1613
Size: 1119 4639
PsiI TTA'TAA
Cuts at: 3328 3328
Size: 5758
PstI C_TGCA'G
Cuts at: 830 3110 830
Size: 2280 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5758
PvuII CAG'CTG
Cuts at: 2416 2685 2416
Size: 269 5489
SacI G_AGCT'C
Cuts at: 2076 2076
Size: 5758
SacII CC_GC'GG
Cuts at: 2232 2232
Size: 5758
SalI G'TCGA_C
Cuts at: 1936 3100 1936
Size: 1164 4594
SbfI CC_TGCA'GG
Cuts at: 3110 3110
Size: 5758
ScaI AGT'ACT
Cuts at: 2840 4006 5718 2840
Size: 1166 1712 2880
Fragments arranged by size:
2880 1712 1166
SfcI C'TryA_G
Cuts at: 826 1045 2829 3106 4241 4922 5113 826
Size: 219 1784 277 1135 681 191 1471
Fragments arranged by size:
1784 1471 1135 681 277 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5758
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5758
SmaI CCC'GGG
Cuts at: 1872 3085 1872
Size: 1213 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2306 3862 4763 5025 820
Size: 197 926 363 1556 901 262 1553
Fragments arranged by size:
1556 1553 926 901 363 262 197
SnaBI TAC'GTA
Cuts at: 493 3549 493
Size: 3056 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5758
SphI G_CATG'C
Cuts at: 1132 3116 1132
Size: 1984 3774
SspI AAT'ATT
Cuts at: 5 52 3682 5
Size: 47 3630 2081
Fragments arranged by size:
3630 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5758
StyI C'CwwG_G
Cuts at: 513 1065 2004 2094 3197 513
Size: 552 939 90 1103 3074
Fragments arranged by size:
3074 1103 939 552 90
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2458 3051 3785 3802 3955 4140 4585
Size: 1255 878 593 734 17 153 185 445
Cuts at: 4585 325
Size: 1498
Fragments arranged by size:
1498 1255 878 734 593 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2625 2838 2886
Size: 274 80 33 51 1443 648 213 48
Cuts at: 2886 4004 5716 96
Size: 1118 1712 138
Fragments arranged by size:
1712 1443 1118 648 274 213 138 80
51 48 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2966 2998 5718 5750 2966
Size: 32 2720 32 2974
Fragments arranged by size:
2974 2720 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5758
XbaI T'CTAG_A
Cuts at: 3094 3094
Size: 5758
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2716 2011
Size: 705 5053
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5758
XmaI C'CCGG_G
Cuts at: 1870 3083 1870
Size: 1213 4545
XmnI GAAnn'nnTTC
Cuts at: 3887 3887
Size: 5758
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3566 276
Size: 53 83 186 936 2032 2468
Fragments arranged by size:
2468 2032 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI
PvuII SacI SacII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI
PsrI RsrII SanDI SapI SexAI SfiI SfoI SrfI
SwaI