Restriction map for
pFN21AE9554
(Circular) (Six-base) MAPSORT of: pFN21AE9554.seq Check: 9819 from: 1 to: 5752
pFN21AE9554 5752 bp
With 182 enzymes: SgfI *
March 28, 2012 12:01 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3562 278
Size: 53 83 186 936 2026 2468
Fragments arranged by size:
2468 2026 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3095 1937
Size: 1158 4594
Acc65I G'GTAC_C
Cuts at: 3073 3073
Size: 5752
AclI AA'CG_TT
Cuts at: 1563 3879 4252 1563
Size: 2316 373 3063
Fragments arranged by size:
3063 2316 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2739 2839 3815 5199 5467 1632
Size: 1107 100 976 1384 268 1917
Fragments arranged by size:
1917 1384 1107 976 268 100
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5555
AflIII A'CryG_T
Cuts at: 1203 2703 4651 5628 1203
Size: 1500 1948 977 1327
Fragments arranged by size:
1948 1500 1327 977
AgeI A'CCGG_T
Cuts at: 4583 4583
Size: 5752
AhdI GACnn_n'nnGTC
Cuts at: 4481 4481
Size: 5752
AleI CACnn'nnGTG
Cuts at: 1166 2114 2306 2988 1166
Size: 948 192 682 3930
Fragments arranged by size:
3930 948 682 192
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2477 1184
Size: 1293 4459
AlwNI CAG_nnn'CTG
Cuts at: 3495 5067 3495
Size: 1572 4180
ApaLI G'TGCA_C
Cuts at: 2417 2611 3809 4965 2417
Size: 194 1198 1156 3204
Fragments arranged by size:
3204 1198 1156 194
ApoI r'AATT_y
Cuts at: 1493 2929 3061 3287 3439 5548 1493
Size: 1436 132 226 152 2109 1697
Fragments arranged by size:
2109 1697 1436 226 152 132
AseI AT'TA_AT
Cuts at: 160 1032 4306 160
Size: 872 3274 1606
Fragments arranged by size:
3274 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5752
AvaI C'yCGr_G
Cuts at: 1870 1943 2072 3077 1870
Size: 73 129 1005 4545
Fragments arranged by size:
4545 1005 129 73
BamHI G'GATC_C
Cuts at: 3082 3449 3082
Size: 367 5385
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2315 3073 4528 618
Size: 325 217 627 528 758 1455 1842
Fragments arranged by size:
1842 1455 758 627 528 325 217
BanII G_rGCy'C
Cuts at: 1391 2058 2073 3071 1391
Size: 667 15 998 4072
Fragments arranged by size:
4072 998 667 15
BbeI G_GCGC'C
Cuts at: 2319 2319
Size: 5752
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2614 928
Size: 971 715 4066
Fragments arranged by size:
4066 971 715
BbvCI CC'TCA_GC
Cuts at: 2027 2027
Size: 5752
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3943 3977 1903
Size: 34 2006 34 3678
Fragments arranged by size:
3678 2006 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3644 4854 3644
Size: 1210 4542
BclI T'GATC_A
Cuts at: 1548 2166 1548
Size: 618 5134
BfrBI ATG'CAT
Cuts at: 2915 2915
Size: 5752
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2249 4363 136
Size: 107 122 71 631 605 577 2114 1525
Fragments arranged by size:
2114 1525 631 605 577 122 107 71
BglII A'GATC_T
Cuts at: 5747 5747
Size: 5752
BlpI GC'TnA_GC
Cuts at: 3169 3169
Size: 5752
Bme1580I G_kGCm'C
Cuts at: 1790 2421 2615 3813 4969 1790
Size: 631 194 1198 1156 2573
Fragments arranged by size:
2573 1198 1156 631 194
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5752
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3483 4441 442
Size: 416 53 83 399 24 2066 958 1753
Fragments arranged by size:
2066 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5752
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4412 1239
Size: 3173 2579
Bpu10I CC'TnA_GC
Cuts at: 2027 2234 2027
Size: 207 5545
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3877 4742 5040 3877
Size: 865 298 4589
Fragments arranged by size:
4589 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4415 882
Size: 617 2916 2219
Fragments arranged by size:
2916 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2407 2605 3543 493
Size: 711 1203 198 938 2702
Fragments arranged by size:
2702 1203 938 711 198
BsaBI GATnn'nnATC
Cuts at: 5746 5746
Size: 5752
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2316 2837 3559 3941
Size: 53 83 186 936 783 521 722 382
Cuts at: 3941 4576 275
Size: 635 1451
Fragments arranged by size:
1451 936 783 722 635 521 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4185 4583 4857 5004 5415 4185
Size: 398 274 147 411 4522
Fragments arranged by size:
4522 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5527
Fragments arranged by size:
5527 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2422 2425 1186
Size: 1236 3 4513
Fragments arranged by size:
4513 1236 3
BseYI C'CCAG_C
Cuts at: 2140 2149 2311 2464 2565 4955 5451 2140
Size: 9 162 153 101 2390 496 2441
Fragments arranged by size:
2441 2390 496 162 153 101 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2724 1163
Size: 573 988 4191
Fragments arranged by size:
4191 988 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2019 3202 3963 4991 1540
Size: 275 186 18 1183 761 1028 2301
Fragments arranged by size:
2301 1183 1028 761 275 186 18
BsiHKAI G_wGCw'C
Cuts at: 2210 2421 2615 3813 3898 4969 2210
Size: 211 194 1198 85 1071 2993
Fragments arranged by size:
2993 1198 1071 211 194 85
BsmI GAATG_Cn'
Cuts at: 3263 3356 3263
Size: 93 5659
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2058 2073 2210 2421 2615 3071 3813
Size: 399 268 15 137 211 194 456 742
Cuts at: 3813 3898 4969 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 456 399 268 211 194
137 85 15
BspEI T'CCGG_A
Cuts at: 5415 5415
Size: 5752
BspHI T'CATG_A
Cuts at: 3641 5371 3641
Size: 1730 4022
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2265 2640 3107 844
Size: 1421 375 467 3489
Fragments arranged by size:
3489 1421 467 375
BsrBI CCG'CTC
Cuts at: 1394 1823 2641 3639 1394
Size: 429 818 998 3507
Fragments arranged by size:
3507 998 818 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2493 4247 4421 65
Size: 1183 1245 1754 174 1396
Fragments arranged by size:
1754 1396 1245 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2839 4396 4583 1700
Size: 110 1029 1557 187 2869
Fragments arranged by size:
2869 1557 1029 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5752
BssHII G'CGCG_C
Cuts at: 1925 2180 2542 1925
Size: 255 362 5135
Fragments arranged by size:
5135 362 255
BssSI C'ACGA_G
Cuts at: 3812 4824 3812
Size: 1012 4740
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5752
BstBI TT'CG_AA
Cuts at: 4556 4556
Size: 5752
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5752
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2500 2011
Size: 489 5263
BstYI r'GATC_y
Cuts at: 1972 3082 3449 3834 3851 5303 5389 5401 5747
Size: 1110 367 385 17 1452 86 12 346
Cuts at: 5747 1972
Size: 1977
Fragments arranged by size:
1977 1452 1110 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2441 2441
Size: 5752
BtgI C'CryG_G
Cuts at: 513 1065 2004 2201 2394 2499 2511 513
Size: 552 939 197 193 105 12 3754
Fragments arranged by size:
3754 939 552 197 193 105 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2224 2402 487
Size: 683 56 998 178 3837
Fragments arranged by size:
3837 998 683 178 56
BtsI GCAGTG_nn'
Cuts at: 2579 2869 3268 4062 4082 2579
Size: 290 399 794 20 4249
Fragments arranged by size:
4249 794 399 290 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5752
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5717
DraI TTT'AAA
Cuts at: 3056 3056
Size: 5752
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4759 809
Size: 507 3443 1802
Fragments arranged by size:
3443 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2048 2382 2409
Size: 54 1425 50 275 18 218 334 27
Cuts at: 2409 3199 4088 5717 8
Size: 790 889 1629 43
Fragments arranged by size:
1629 1425 889 790 334 275 218 54
50 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 3199 1537
Size: 275 1387 4090
Fragments arranged by size:
4090 1387 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2267 2537 2858 3682 1353
Size: 914 270 321 824 3423
Fragments arranged by size:
3423 914 824 321 270
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4339 4713 4859 387
Size: 1064 138 2750 374 146 1280
Fragments arranged by size:
2750 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2739 2839 3815 4412 5199 5467 1239
Size: 393 1107 100 976 597 787 268 1524
Fragments arranged by size:
1524 1107 976 787 597 393 268 100
EcoNI CCTnn'n_nnAGG
Cuts at: 2030 2440 2030
Size: 410 5342
EcoO109I rG'GnC_Cy
Cuts at: 2527 2671 2527
Size: 144 5608
EcoRI G'AATT_C
Cuts at: 3061 3439 3061
Size: 378 5374
FspI TGC'GCA
Cuts at: 4258 4258
Size: 5752
HaeII r_GCGC'y
Cuts at: 1678 2319 2431 2489 4899 1678
Size: 641 112 58 2410 2531
Fragments arranged by size:
2531 2410 641 112 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4393 4425 4467 4499 1373
Size: 32 135 32 2821 32 42 32 2626
Fragments arranged by size:
2821 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3096 3342 669
Size: 988 281 1158 246 3079
Fragments arranged by size:
3079 1158 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5752
HpaI GTT'AAC
Cuts at: 3342 3342
Size: 5752
KasI G'GCGC_C
Cuts at: 2315 2315
Size: 5752
KpnI G_GTAC'C
Cuts at: 3077 3077
Size: 5752
MfeI C'AATT_G
Cuts at: 3037 3351 3037
Size: 314 5438
MluI A'CGCG_T
Cuts at: 5628 5628
Size: 5752
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4866 5050 1305
Size: 3561 184 2007
Fragments arranged by size:
3561 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2411 10
Size: 54 1425 343 579 3351
Fragments arranged by size:
3351 1425 579 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2093 2114 2306 2363 2498 2988 3710
Size: 648 927 21 192 57 135 490 722
Cuts at: 3710 4069 4228 518
Size: 359 159 2042
Fragments arranged by size:
2042 927 722 648 490 359 192 159
135 57 21
MspA1I CmG'CkG
Cuts at: 1606 2080 2203 2833 3168 3845 4993 5238 1606
Size: 474 123 630 335 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 630 474 335 245 123
NaeI GCC'GGC
Cuts at: 2841 2841
Size: 5752
NarI GG'CG_CC
Cuts at: 2316 2316
Size: 5752
NcoI C'CATG_G
Cuts at: 513 1065 2004 2499 513
Size: 552 939 495 3766
Fragments arranged by size:
3766 939 552 495
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5752
NgoMIV G'CCGG_C
Cuts at: 2839 2839
Size: 5752
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5752
NotI GC'GGCC_GC
Cuts at: 3199 3199
Size: 5752
NsiI A_TGCA'T
Cuts at: 2917 2917
Size: 5752
NspI r_CATG'y
Cuts at: 1132 1229 2707 3110 4655 1132
Size: 97 1478 403 1545 2229
Fragments arranged by size:
2229 1545 1478 403 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5752
PciI A'CATG_T
Cuts at: 2703 4651 2703
Size: 1948 3804
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2875 1108
Size: 1767 3985
PmeI GTTT'AAAC
Cuts at: 3056 3056
Size: 5752
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3821 3853 5334 5366 3821
Size: 32 1481 32 4207
Fragments arranged by size:
4207 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2671 2671
Size: 5752
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5752
PsiI TTA'TAA
Cuts at: 3322 3322
Size: 5752
PstI C_TGCA'G
Cuts at: 830 3104 830
Size: 2274 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5752
SacII CC_GC'GG
Cuts at: 2204 2204
Size: 5752
SalI G'TCGA_C
Cuts at: 1936 3094 1936
Size: 1158 4594
SapI GCTCTTCn'nnn_
Cuts at: 2267 2267
Size: 5752
SbfI CC_TGCA'GG
Cuts at: 3104 3104
Size: 5752
ScaI AGT'ACT
Cuts at: 4000 5712 4000
Size: 1712 4040
SfcI C'TryA_G
Cuts at: 826 1045 3100 4235 4916 5107 826
Size: 219 2055 1135 681 191 1471
Fragments arranged by size:
2055 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2317 2317
Size: 5752
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5752
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5752
SmaI CCC'GGG
Cuts at: 1872 2074 3079 1872
Size: 202 1005 4545
Fragments arranged by size:
4545 1005 202
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3856 4757 5019 820
Size: 197 926 1913 901 262 1553
Fragments arranged by size:
1913 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3543 493
Size: 3050 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5752
SphI G_CATG'C
Cuts at: 1132 3110 1132
Size: 1978 3774
SrfI GCCC'GGGC
Cuts at: 2074 2074
Size: 5752
SspI AAT'ATT
Cuts at: 5 52 3676 5
Size: 47 3624 2081
Fragments arranged by size:
3624 2081 47
StuI AGG'CCT
Cuts at: 1517 2600 2942 1517
Size: 1083 342 4327
Fragments arranged by size:
4327 1083 342
StyI C'CwwG_G
Cuts at: 513 1065 2004 2499 2667 3191 513
Size: 552 939 495 168 524 3074
Fragments arranged by size:
3074 939 552 524 495 168
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2296 3779 3796 3949 4134 4579 325
Size: 1255 716 1483 17 153 185 445 1498
Fragments arranged by size:
1498 1483 1255 716 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3041 3998 5710
Size: 274 80 33 51 1443 1064 957 1712
Cuts at: 5710 96
Size: 138
Fragments arranged by size:
1712 1443 1064 957 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5712 5744 5712
Size: 32 5720
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5752
XbaI T'CTAG_A
Cuts at: 3088 3088
Size: 5752
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2308 2308
Size: 5752
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5752
XmaI C'CCGG_G
Cuts at: 1870 2072 3077 1870
Size: 202 1005 4545
Fragments arranged by size:
4545 1005 202
XmnI GAAnn'nnTTC
Cuts at: 3881 3881
Size: 5752
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3560 276
Size: 53 83 186 936 2026 2468
Fragments arranged by size:
2468 2026 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SacII SalI SapI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BplI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI
FspAI NruI PacI PfoI PmlI PspOMI PspXI PsrI
PvuII RsrII SacI SanDI SexAI SfiI SwaI