Restriction map for
pFN21AE9553
(Circular) (Six-base) MAPSORT of: pFN21AE9553.seq Check: 8177 from: 1 to: 6823
pFN21AE9553 6823 bp
With 182 enzymes: SgfI *
December 28, 2011 15:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4633 278
Size: 53 83 186 936 3097 2468
Fragments arranged by size:
3097 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3903 4166 1937
Size: 1966 263 4594
Fragments arranged by size:
4594 1966 263
Acc65I G'GTAC_C
Cuts at: 4144 4144
Size: 6823
AclI AA'CG_TT
Cuts at: 1563 4950 5323 1563
Size: 3387 373 3063
Fragments arranged by size:
3387 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3247 3677 4011 4886 6270 6538 1632
Size: 1615 430 334 875 1384 268 1917
Fragments arranged by size:
1917 1615 1384 875 430 334 268
AflII C'TTAA_G
Cuts at: 820 1017 2498 820
Size: 197 1481 5145
Fragments arranged by size:
5145 1481 197
AflIII A'CryG_T
Cuts at: 1203 2030 2565 5722 6699 1203
Size: 827 535 3157 977 1327
Fragments arranged by size:
3157 1327 977 827 535
AgeI A'CCGG_T
Cuts at: 5654 5654
Size: 6823
AhdI GACnn_n'nnGTC
Cuts at: 5552 5552
Size: 6823
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6823
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4025 1184
Size: 2841 3982
AlwNI CAG_nnn'CTG
Cuts at: 4121 4566 6138 4121
Size: 445 1572 4806
Fragments arranged by size:
4806 1572 445
ApaLI G'TGCA_C
Cuts at: 4880 6036 4880
Size: 1156 5667
ApoI r'AATT_y
Cuts at: 1493 2268 2429 3239 4132 4358 4510 6619 1493
Size: 775 161 810 893 226 152 2109 1697
Fragments arranged by size:
2109 1697 893 810 775 226 161 152
AseI AT'TA_AT
Cuts at: 160 1032 2330 3455 5377 160
Size: 872 1298 1125 1922 1606
Fragments arranged by size:
1922 1606 1298 1125 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6823
AvaI C'yCGr_G
Cuts at: 1870 1943 4148 1870
Size: 73 2205 4545
Fragments arranged by size:
4545 2205 73
AvrII C'CTAG_G
Cuts at: 2827 3745 2827
Size: 918 5905
BamHI G'GATC_C
Cuts at: 2346 4153 4520 2346
Size: 1807 367 4649
Fragments arranged by size:
4649 1807 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4144 5599 618
Size: 325 217 627 2357 1455 1842
Fragments arranged by size:
2357 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3153 4142 1391
Size: 1762 989 4072
Fragments arranged by size:
4072 1762 989
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3253 3469 928
Size: 971 1354 216 4282
Fragments arranged by size:
4282 1354 971 216
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5014 5048 1903
Size: 34 3077 34 3678
Fragments arranged by size:
3678 3077 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4715 5925 4715
Size: 1210 5613
BclI T'GATC_A
Cuts at: 1548 3358 1548
Size: 1810 5013
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3921 5434 136
Size: 107 122 71 631 605 2249 1513 1525
Fragments arranged by size:
2249 1525 1513 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6818 6818
Size: 6823
BlpI GC'TnA_GC
Cuts at: 4240 4240
Size: 6823
Bme1580I G_kGCm'C
Cuts at: 1790 2061 4884 6040 1790
Size: 271 2823 1156 2573
Fragments arranged by size:
2823 2573 1156 271
BmgBI CAC'GTC
Cuts at: 1318 2031 1318
Size: 713 6110
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4014 4554 5512
Size: 416 53 83 399 24 2597 540 958
Cuts at: 5512 442
Size: 1753
Fragments arranged by size:
2597 1753 958 540 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 3822 1056
Size: 2766 4057
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3865 5483 1239
Size: 2626 1618 2579
Fragments arranged by size:
2626 2579 1618
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4948 5813 6111 4948
Size: 865 298 5660
Fragments arranged by size:
5660 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2692 3358 5486 882
Size: 617 1193 666 2128 2219
Fragments arranged by size:
2219 2128 1193 666 617
BsaAI yAC'GTr
Cuts at: 493 1204 4614 493
Size: 711 3410 2702
Fragments arranged by size:
3410 2702 711
BsaBI GATnn'nnATC
Cuts at: 6817 6817
Size: 6823
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4630 5012 5647 275
Size: 53 83 186 936 3097 382 635 1451
Fragments arranged by size:
3097 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5256 5654 5928 6075 6486 5256
Size: 398 274 147 411 5593
Fragments arranged by size:
5593 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6598
Fragments arranged by size:
6598 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2375 1186
Size: 1189 5634
BseYI C'CCAG_C
Cuts at: 3515 6026 6522 3515
Size: 2511 496 3816
Fragments arranged by size:
3816 2511 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6250
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4273 5034 6062 1540
Size: 275 186 2272 761 1028 2301
Fragments arranged by size:
2301 2272 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4884 4969 6040 4884
Size: 85 1071 5667
Fragments arranged by size:
5667 1071 85
BsmI GAATG_Cn'
Cuts at: 4334 4427 4334
Size: 93 6730
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2061 3153 4142 4884 4969 6040 1391
Size: 399 271 1092 989 742 85 1071 2174
Fragments arranged by size:
2174 1092 1071 989 742 399 271 85
BspEI T'CCGG_A
Cuts at: 6486 6486
Size: 6823
BspHI T'CATG_A
Cuts at: 4712 6442 4712
Size: 1730 5093
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2264 3865 4003 4178 844
Size: 1420 1601 138 175 3489
Fragments arranged by size:
3489 1601 1420 175 138
BsrBI CCG'CTC
Cuts at: 1394 1823 4710 1394
Size: 429 2887 3507
Fragments arranged by size:
3507 2887 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5318 5492 65
Size: 1183 4070 174 1396
Fragments arranged by size:
4070 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5467 5654 1700
Size: 110 3657 187 2869
Fragments arranged by size:
3657 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6823
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6823
BssSI C'ACGA_G
Cuts at: 4883 5895 4883
Size: 1012 5811
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2781 794
Size: 1987 4836
BstBI TT'CG_AA
Cuts at: 3250 5627 3250
Size: 2377 4446
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6823
BstXI CCAn_nnnn'nTGG
Cuts at: 3676 3676
Size: 6823
BstYI r'GATC_y
Cuts at: 1972 2346 3544 4153 4520 4905 4922 6374 6460
Size: 374 1198 609 367 385 17 1452 86
Cuts at: 6460 6472 6818 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1198 609 385 374 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2358 2358
Size: 6823
BtgI C'CryG_G
Cuts at: 513 1065 2004 2166 3675 513
Size: 552 939 162 1509 3661
Fragments arranged by size:
3661 1509 939 552 162
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6084
Fragments arranged by size:
6084 683 56
BtsI GCAGTG_nn'
Cuts at: 2982 3931 4339 5133 5153 2982
Size: 949 408 794 20 4652
Fragments arranged by size:
4652 949 794 408 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6823
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3046 3081 529
Size: 35 2482 35 4271
Fragments arranged by size:
4271 2482 35 35
DraI TTT'AAA
Cuts at: 2575 3526 4127 2575
Size: 951 601 5271
Fragments arranged by size:
5271 951 601
DraIII CAC_nnn'GTG
Cuts at: 2898 2898
Size: 6823
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2822 5830 809
Size: 507 1506 3008 1802
Fragments arranged by size:
3008 1802 1506 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3214 4270 5159
Size: 54 1425 50 275 18 1384 1056 889
Cuts at: 5159 6788 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1384 1056 889 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4270 1537
Size: 275 2458 4090
Fragments arranged by size:
4090 2458 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2072 3162 3559 4753 1353
Size: 719 1090 397 1194 3423
Fragments arranged by size:
3423 1194 1090 719 397
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5410 5784 5930 387
Size: 1064 138 3821 374 146 1280
Fragments arranged by size:
3821 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3247 3677 3865 4011 4886 5483 6270
Size: 393 1615 430 188 146 875 597 787
Cuts at: 6270 6538 1239
Size: 268 1524
Fragments arranged by size:
1615 1524 875 787 597 430 393 268
188 146
EcoO109I rG'GnC_Cy
Cuts at: 4015 4015
Size: 6823
EcoRI G'AATT_C
Cuts at: 4132 4510 4132
Size: 378 6445
FspI TGC'GCA
Cuts at: 5329 5329
Size: 6823
HaeII r_GCGC'y
Cuts at: 1678 5970 1678
Size: 4292 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3294 3326 3534 3566 5464
Size: 32 135 32 1722 32 208 32 1898
Cuts at: 5464 5496 5538 5570 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1898 1722 208 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2028 3904 4167 4413 669
Size: 988 281 90 1876 263 246 3079
Fragments arranged by size:
3079 1876 988 281 263 246 90
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6823
HpaI GTT'AAC
Cuts at: 4413 4413
Size: 6823
KpnI G_GTAC'C
Cuts at: 4148 4148
Size: 6823
MfeI C'AATT_G
Cuts at: 4422 4422
Size: 6823
MluI A'CGCG_T
Cuts at: 6699 6699
Size: 6823
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3466 5937 6121 1305
Size: 2161 2471 184 2007
Fragments arranged by size:
2471 2161 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5001
Fragments arranged by size:
5001 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 4781 5140 5299 518
Size: 648 843 2772 359 159 2042
Fragments arranged by size:
2772 2042 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2168 3267 4239 4916 6064 6309 1606
Size: 562 1099 972 677 1148 245 2120
Fragments arranged by size:
2120 1148 1099 972 677 562 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3675 513
Size: 552 939 1671 3661
Fragments arranged by size:
3661 1671 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6823
NheI G'CTAG_C
Cuts at: 1052 3818 1052
Size: 2766 4057
NotI GC'GGCC_GC
Cuts at: 4270 4270
Size: 6823
NspI r_CATG'y
Cuts at: 1132 1229 2569 3504 4181 5726 1132
Size: 97 1340 935 677 1545 2229
Fragments arranged by size:
2229 1545 1340 935 677 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6823
PciI A'CATG_T
Cuts at: 2565 5722 2565
Size: 3157 3666
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2922 1108
Size: 1814 5009
PmeI GTTT'AAAC
Cuts at: 4127 4127
Size: 6823
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4892 4924 6405 6437 4892
Size: 32 1481 32 5278
Fragments arranged by size:
5278 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 4015 4015
Size: 6823
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6823
PsiI TTA'TAA
Cuts at: 4393 4393
Size: 6823
PstI C_TGCA'G
Cuts at: 830 2938 3721 4175 830
Size: 2108 783 454 3478
Fragments arranged by size:
3478 2108 783 454
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6823
SacII CC_GC'GG
Cuts at: 2169 2169
Size: 6823
SalI G'TCGA_C
Cuts at: 1936 3902 4165 1936
Size: 1966 263 4594
Fragments arranged by size:
4594 1966 263
SbfI CC_TGCA'GG
Cuts at: 4175 4175
Size: 6823
ScaI AGT'ACT
Cuts at: 3570 5071 6783 3570
Size: 1501 1712 3610
Fragments arranged by size:
3610 1712 1501
SexAI A'CCwGG_T
Cuts at: 3007 3007
Size: 6823
SfcI C'TryA_G
Cuts at: 826 1045 2934 3572 3696 3717 4171 5306 5987
Size: 219 1889 638 124 21 454 1135 681
Cuts at: 5987 6178 826
Size: 191 1471
Fragments arranged by size:
1889 1471 1135 681 638 454 219 191
124 21
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6823
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6823
SmaI CCC'GGG
Cuts at: 1872 4150 1872
Size: 2278 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2498 4927 5828 6090 820
Size: 197 926 555 2429 901 262 1553
Fragments arranged by size:
2429 1553 926 901 555 262 197
SnaBI TAC'GTA
Cuts at: 493 4614 493
Size: 4121 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6823
SphI G_CATG'C
Cuts at: 1132 4181 1132
Size: 3049 3774
SspI AAT'ATT
Cuts at: 5 52 2625 4747 5
Size: 47 2573 2122 2081
Fragments arranged by size:
2573 2122 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6823
StyI C'CwwG_G
Cuts at: 513 1065 2004 2489 2827 3675 3745 4262 513
Size: 552 939 485 338 848 70 517 3074
Fragments arranged by size:
3074 939 848 552 517 485 338 70
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2893 4850 4867 5020 5205 5650 325
Size: 1255 1313 1957 17 153 185 445 1498
Fragments arranged by size:
1957 1498 1313 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2745 3115 3568
Size: 274 80 33 51 1443 768 370 453
Cuts at: 3568 5069 6781 96
Size: 1501 1712 138
Fragments arranged by size:
1712 1501 1443 768 453 370 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2997 3029 6783 6815 2997
Size: 32 3754 32 3005
Fragments arranged by size:
3754 3005 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6823
XbaI T'CTAG_A
Cuts at: 4159 4159
Size: 6823
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2471 2471
Size: 6823
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6823
XmaI C'CCGG_G
Cuts at: 1870 4148 1870
Size: 2278 4545
XmnI GAAnn'nnTTC
Cuts at: 2268 2617 4952 2268
Size: 349 2335 4139
Fragments arranged by size:
4139 2335 349
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4631 276
Size: 53 83 186 936 3097 2468
Fragments arranged by size:
3097 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SacII SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI
PvuII RsrII SacI SanDI SapI SfiI SfoI SrfI
SwaI