Restriction map for
pFN21AE9544
(Circular) (Six-base) MAPSORT of: pFN21AE9544.seq Check: 2641 from: 1 to: 6103
pFN21AE9544 6103 bp
With 182 enzymes: SgfI *
July 6, 2012 17:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3913 278
Size: 53 83 186 936 2377 2468
Fragments arranged by size:
2468 2377 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2721 3446 1937
Size: 784 725 4594
Fragments arranged by size:
4594 784 725
Acc65I G'GTAC_C
Cuts at: 2127 2262 2838 3424 2127
Size: 135 576 586 4806
Fragments arranged by size:
4806 586 576 135
AclI AA'CG_TT
Cuts at: 1563 4230 4603 1563
Size: 2667 373 3063
Fragments arranged by size:
3063 2667 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2157 4166 5550 5818 1632
Size: 525 2009 1384 268 1917
Fragments arranged by size:
2009 1917 1384 525 268
AfeI AGC'GCT
Cuts at: 2117 2117
Size: 6103
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5906
AflIII A'CryG_T
Cuts at: 1203 2697 5002 5979 1203
Size: 1494 2305 977 1327
Fragments arranged by size:
2305 1494 1327 977
AgeI A'CCGG_T
Cuts at: 4934 4934
Size: 6103
AhdI GACnn_n'nnGTC
Cuts at: 4832 4832
Size: 6103
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6103
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6103
AlwNI CAG_nnn'CTG
Cuts at: 2032 3365 3846 5418 2032
Size: 1333 481 1572 2717
Fragments arranged by size:
2717 1572 1333 481
ApaI G_GGCC'C
Cuts at: 2803 3295 2803
Size: 492 5611
ApaLI G'TGCA_C
Cuts at: 4160 5316 4160
Size: 1156 4947
ApoI r'AATT_y
Cuts at: 1493 3412 3638 3790 5899 1493
Size: 1919 226 152 2109 1697
Fragments arranged by size:
2109 1919 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 2931 4657 160
Size: 872 1899 1726 1606
Fragments arranged by size:
1899 1726 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6103
AvaI C'yCGr_G
Cuts at: 1870 1943 3099 3313 3346 3428 1870
Size: 73 1156 214 33 82 4545
Fragments arranged by size:
4545 1156 214 82 73 33
BamHI G'GATC_C
Cuts at: 3433 3800 3433
Size: 367 5736
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2127 2262 2274 2687 2838
Size: 325 217 627 340 135 12 413 151
Cuts at: 2838 3043 3104 3225 3424 4879 618
Size: 205 61 121 199 1455 1842
Fragments arranged by size:
1842 1455 627 413 340 325 217 205
199 151 135 121 61 12
BanII G_rGCy'C
Cuts at: 1391 2803 3295 3422 1391
Size: 1412 492 127 4072
Fragments arranged by size:
4072 1412 492 127
BbeI G_GCGC'C
Cuts at: 3108 3108
Size: 6103
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5132
BbvCI CC'TCA_GC
Cuts at: 2978 3014 3143 2978
Size: 36 129 5938
Fragments arranged by size:
5938 129 36
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4294 4328 1903
Size: 34 2357 34 3678
Fragments arranged by size:
3678 2357 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3995 5205 3995
Size: 1210 4893
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6103
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2313 3290 4714
Size: 107 122 71 631 605 641 977 1424
Cuts at: 4714 136
Size: 1525
Fragments arranged by size:
1525 1424 977 641 631 605 122 107
71
BglII A'GATC_T
Cuts at: 3135 6098 3135
Size: 2963 3140
BlpI GC'TnA_GC
Cuts at: 3520 3520
Size: 6103
Bme1580I G_kGCm'C
Cuts at: 1790 2803 3048 3295 4164 5320 1790
Size: 1013 245 247 869 1156 2573
Fragments arranged by size:
2573 1156 1013 869 247 245
BmgBI CAC'GTC
Cuts at: 1318 2538 1318
Size: 1220 4883
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2668 3834 4792
Size: 416 53 83 399 24 1251 1166 958
Cuts at: 4792 442
Size: 1753
Fragments arranged by size:
1753 1251 1166 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2468 1056
Size: 1412 4691
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2098 3039 2098
Size: 941 5162
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2048 4763 1239
Size: 809 2715 2579
Fragments arranged by size:
2715 2579 809
Bpu10I CC'TnA_GC
Cuts at: 2978 3014 3143 2978
Size: 36 129 5938
Fragments arranged by size:
5938 129 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2500 4228 5093 5391 2500
Size: 1728 865 298 3212
Fragments arranged by size:
3212 1728 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2141 2337 2568 4766 882
Size: 617 642 196 231 2198 2219
Fragments arranged by size:
2219 2198 642 617 231 196
BsaAI yAC'GTr
Cuts at: 493 1204 2700 3894 493
Size: 711 1496 1194 2702
Fragments arranged by size:
2702 1496 1194 711
BsaBI GATnn'nnATC
Cuts at: 6097 6097
Size: 6103
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2718 3105 3910 4292
Size: 53 83 186 936 1185 387 805 382
Cuts at: 4292 4927 275
Size: 635 1451
Fragments arranged by size:
1451 1185 936 805 635 387 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4536 4934 5208 5355 5766 4536
Size: 398 274 147 411 4873
Fragments arranged by size:
4873 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1999 2029 3188 3218 3255
Size: 30 165 30 596 30 1159 30 37
Cuts at: 3255 3285 1178
Size: 30 3996
Fragments arranged by size:
3996 1159 596 165 37 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2774 2777 3272 1186
Size: 1588 3 495 4017
Fragments arranged by size:
4017 1588 495 3
BseYI C'CCAG_C
Cuts at: 2118 2214 2793 2862 3052 5306 5802 2118
Size: 96 579 69 190 2254 496 2419
Fragments arranged by size:
2419 2254 579 496 190 96 69
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2211 2711 3221 1163
Size: 573 475 500 510 4045
Fragments arranged by size:
4045 573 510 500 475
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3553 4314 5342 1540
Size: 275 186 1552 761 1028 2301
Fragments arranged by size:
2301 1552 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2745 4164 4249 5320 2745
Size: 1419 85 1071 3528
Fragments arranged by size:
3528 1419 1071 85
BsmI GAATG_Cn'
Cuts at: 2952 2961 3614 3707 2952
Size: 9 653 93 5348
Fragments arranged by size:
5348 653 93 9
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2745 2803 3048 3295 3422 4164 4249
Size: 399 955 58 245 247 127 742 85
Cuts at: 4249 5320 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 955 742 399 247 245 127
85 58
BspEI T'CCGG_A
Cuts at: 5766 5766
Size: 6103
BspHI T'CATG_A
Cuts at: 2916 3992 5722 2916
Size: 1076 1730 3297
Fragments arranged by size:
3297 1730 1076
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2274 2282 2407 2733 2790 3458 844
Size: 1430 8 125 326 57 668 3489
Fragments arranged by size:
3489 1430 668 326 125 57 8
BsrBI CCG'CTC
Cuts at: 1394 1823 2410 3990 1394
Size: 429 587 1580 3507
Fragments arranged by size:
3507 1580 587 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2500 4598 4772 65
Size: 1183 1252 2098 174 1396
Fragments arranged by size:
2098 1396 1252 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3009 4747 4934 1700
Size: 110 1199 1738 187 2869
Fragments arranged by size:
2869 1738 1199 187 110
BsrGI T'GTAC_A
Cuts at: 96 2424 96
Size: 2328 3775
BssHII G'CGCG_C
Cuts at: 1925 3280 1925
Size: 1355 4748
BssSI C'ACGA_G
Cuts at: 2744 3062 4163 5175 2744
Size: 318 1101 1012 3672
Fragments arranged by size:
3672 1101 1012 318
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2032 2139 2234 794
Size: 1238 107 95 4663
Fragments arranged by size:
4663 1238 107 95
BstBI TT'CG_AA
Cuts at: 4907 4907
Size: 6103
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6103
BstYI r'GATC_y
Cuts at: 1972 3135 3210 3433 3800 4185 4202 5654 5740
Size: 1163 75 223 367 385 17 1452 86
Cuts at: 5740 5752 6098 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1163 385 367 346 223 86
75 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2160 3217 3334 513
Size: 552 1095 1057 117 3282
Fragments arranged by size:
3282 1095 1057 552 117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5364
Fragments arranged by size:
5364 683 56
BtsI GCAGTG_nn'
Cuts at: 2238 2323 2456 2864 3229 3619 4413 4433 2238
Size: 85 133 408 365 390 794 20 3908
Fragments arranged by size:
3908 794 408 390 365 133 85 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6103
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6068
DraI TTT'AAA
Cuts at: 3407 3407
Size: 6103
DraIII CAC_nnn'GTG
Cuts at: 2284 2357 2859 3067 2284
Size: 73 502 208 5320
Fragments arranged by size:
5320 502 208 73
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5110 809
Size: 507 3794 1802
Fragments arranged by size:
3794 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2323 2559 3550
Size: 54 1425 50 275 18 493 236 991
Cuts at: 3550 4439 6068 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 991 889 493 275 236 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3550 1537
Size: 275 1738 4090
Fragments arranged by size:
4090 1738 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4033 1353
Size: 2680 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2087 2294 3203 4690 5064 5210
Size: 1064 138 498 207 909 1487 374 146
Cuts at: 5210 387
Size: 1280
Fragments arranged by size:
1487 1280 1064 909 498 374 207 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2048 2157 4166 4763 5550 5818 1239
Size: 393 416 109 2009 597 787 268 1524
Fragments arranged by size:
2009 1524 787 597 416 393 268 109
EcoNI CCTnn'n_nnAGG
Cuts at: 3254 3254
Size: 6103
EcoO109I rG'GnC_Cy
Cuts at: 2193 2617 2638 2799 2800 3292 3322 3351 3362
Size: 424 21 161 1 492 30 29 11
Cuts at: 3362 2193
Size: 4934
Fragments arranged by size:
4934 492 424 161 30 29 21 11
1
EcoRI G'AATT_C
Cuts at: 3412 3790 3412
Size: 378 5725
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3145 3145
Size: 6103
FspI TGC'GCA
Cuts at: 4609 4609
Size: 6103
HaeII r_GCGC'y
Cuts at: 1678 2119 3108 5250 1678
Size: 441 989 2142 2531
Fragments arranged by size:
2531 2142 989 441
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 1999 2031 3188 3220 4744
Size: 32 135 32 427 32 1157 32 1524
Cuts at: 4744 4776 4818 4850 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1524 1157 427 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3447 3693 669
Size: 988 281 1509 246 3079
Fragments arranged by size:
3079 1509 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6103
HpaI GTT'AAC
Cuts at: 3693 3693
Size: 6103
KasI G'GCGC_C
Cuts at: 3104 3104
Size: 6103
KpnI G_GTAC'C
Cuts at: 2131 2266 2842 3428 2131
Size: 135 576 586 4806
Fragments arranged by size:
4806 586 576 135
MfeI C'AATT_G
Cuts at: 3702 3702
Size: 6103
MluI A'CGCG_T
Cuts at: 5979 5979
Size: 6103
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3186 3369 5217 5401 1305
Size: 1881 183 1848 184 2007
Fragments arranged by size:
2007 1881 1848 184 183
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2325 10
Size: 54 1425 343 493 3788
Fragments arranged by size:
3788 1425 493 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2280 2915 4061 4420 4579 518
Size: 648 1114 635 1146 359 159 2042
Fragments arranged by size:
2042 1146 1114 648 635 359 159
MspA1I CmG'CkG
Cuts at: 1606 2029 2162 3336 3519 4196 5344 5589 1606
Size: 423 133 1174 183 677 1148 245 2120
Fragments arranged by size:
2120 1174 1148 677 423 245 183 133
NarI GG'CG_CC
Cuts at: 3105 3105
Size: 6103
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5551
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6103
NheI G'CTAG_C
Cuts at: 1052 2464 1052
Size: 1412 4691
NotI GC'GGCC_GC
Cuts at: 3550 3550
Size: 6103
NspI r_CATG'y
Cuts at: 1132 1229 3461 5006 1132
Size: 97 2232 1545 2229
Fragments arranged by size:
2232 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2823 3359 1348
Size: 1475 536 4092
Fragments arranged by size:
4092 1475 536
PciI A'CATG_T
Cuts at: 5002 5002
Size: 6103
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2284 2892 3067 3365 1108
Size: 1176 608 175 298 3846
Fragments arranged by size:
3846 1176 608 298 175
PfoI T'CCnGG_A
Cuts at: 3273 3273
Size: 6103
PmeI GTTT'AAAC
Cuts at: 3407 3407
Size: 6103
PmlI CAC'GTG
Cuts at: 2700 2700
Size: 6103
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4172 4204 5685 5717 4172
Size: 32 1481 32 4558
Fragments arranged by size:
4558 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2193 3322 3351 2193
Size: 1129 29 4945
Fragments arranged by size:
4945 1129 29
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6103
PsiI TTA'TAA
Cuts at: 3673 3673
Size: 6103
PspOMI G'GGCC_C
Cuts at: 2799 3291 2799
Size: 492 5611
PstI C_TGCA'G
Cuts at: 830 2028 2293 3455 830
Size: 1198 265 1162 3478
Fragments arranged by size:
3478 1198 1162 265
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6103
PvuII CAG'CTG
Cuts at: 2029 2029
Size: 6103
SacII CC_GC'GG
Cuts at: 2163 3337 2163
Size: 1174 4929
SalI G'TCGA_C
Cuts at: 1936 3445 1936
Size: 1509 4594
SanDI GG'GwC_CC
Cuts at: 3351 3351
Size: 6103
SbfI CC_TGCA'GG
Cuts at: 3455 3455
Size: 6103
ScaI AGT'ACT
Cuts at: 2678 4351 6063 2678
Size: 1673 1712 2718
Fragments arranged by size:
2718 1712 1673
SfcI C'TryA_G
Cuts at: 826 1045 2024 2289 3451 4586 5267 5458 826
Size: 219 979 265 1162 1135 681 191 1471
Fragments arranged by size:
1471 1162 1135 979 681 265 219 191
SfoI GGC'GCC
Cuts at: 3106 3106
Size: 6103
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6103
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6103
SmaI CCC'GGG
Cuts at: 1872 3101 3315 3430 1872
Size: 1229 214 115 4545
Fragments arranged by size:
4545 1229 214 115
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2479 4207 5108 5370 820
Size: 197 926 536 1728 901 262 1553
Fragments arranged by size:
1728 1553 926 901 536 262 197
SnaBI TAC'GTA
Cuts at: 493 3894 493
Size: 3401 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6103
SphI G_CATG'C
Cuts at: 1132 3461 1132
Size: 2329 3774
SrfI GCCC'GGGC
Cuts at: 3315 3315
Size: 6103
SspI AAT'ATT
Cuts at: 5 52 4027 5
Size: 47 3975 2081
Fragments arranged by size:
3975 2081 47
StuI AGG'CCT
Cuts at: 1517 2178 2381 1517
Size: 661 203 5239
Fragments arranged by size:
5239 661 203
StyI C'CwwG_G
Cuts at: 513 1065 2151 2605 3542 513
Size: 552 1086 454 937 3074
Fragments arranged by size:
3074 1086 937 552 454
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2245 2675 2815 3044 4130 4147 4300
Size: 1255 665 430 140 229 1086 17 153
Cuts at: 4300 4485 4930 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1086 665 445 430 229 185
153 140 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2424 2676 4349
Size: 274 80 33 51 1443 447 252 1673
Cuts at: 4349 6061 96
Size: 1712 138
Fragments arranged by size:
1712 1673 1443 447 274 252 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6063 6095 6063
Size: 32 6071
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6103
XbaI T'CTAG_A
Cuts at: 3439 3439
Size: 6103
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3037 3385 3037
Size: 348 5755
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6103
XmaI C'CCGG_G
Cuts at: 1870 3099 3313 3428 1870
Size: 1229 214 115 4545
Fragments arranged by size:
4545 1229 214 115
XmnI GAAnn'nnTTC
Cuts at: 4232 4232
Size: 6103
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3911 276
Size: 53 83 186 936 2377 2468
Fragments arranged by size:
2468 2377 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstXI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NaeI
NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacI
SapI SexAI SfiI SwaI