(Circular) (Six-base) MAPSORT of: pFN21AE9541.seq Check: 1742 from: 1 to: 6049 pFN21AE9541 6049 bp With 182 enzymes: SgfI * March 28, 2012 12:07 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2052 2427 2052 Size: 375 5674 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3859 278 Size: 53 83 186 936 2323 2468 Fragments arranged by size: 2468 2323 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3333 3392 1937 Size: 1396 59 4594 Fragments arranged by size: 4594 1396 59 Acc65I G'GTAC_C Cuts at: 3370 3370 Size: 6049 AclI AA'CG_TT Cuts at: 1563 4176 4549 1563 Size: 2613 373 3063 Fragments arranged by size: 3063 2613 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2607 4112 5496 5764 1632 Size: 975 1505 1384 268 1917 Fragments arranged by size: 1917 1505 1384 975 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5852 AflIII A'CryG_T Cuts at: 1203 3306 4948 5925 1203 Size: 2103 1642 977 1327 Fragments arranged by size: 2103 1642 1327 977 AgeI A'CCGG_T Cuts at: 4880 4880 Size: 6049 AhdI GACnn_n'nnGTC Cuts at: 4778 4778 Size: 6049 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6049 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2300 1184 Size: 1116 4933 AlwNI CAG_nnn'CTG Cuts at: 2263 2696 2810 3792 5364 2263 Size: 433 114 982 1572 2948 Fragments arranged by size: 2948 1572 982 433 114 ApaLI G'TGCA_C Cuts at: 4106 5262 4106 Size: 1156 4893 ApoI r'AATT_y Cuts at: 1493 3358 3584 3736 5845 1493 Size: 1865 226 152 2109 1697 Fragments arranged by size: 2109 1865 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4603 160 Size: 872 3571 1606 Fragments arranged by size: 3571 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6049 AvaI C'yCGr_G Cuts at: 1870 1943 2221 2513 3374 1870 Size: 73 278 292 861 4545 Fragments arranged by size: 4545 861 292 278 73 BamHI G'GATC_C Cuts at: 2711 3379 3746 2711 Size: 668 367 5014 Fragments arranged by size: 5014 668 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2301 2540 2789 2840 2931 Size: 325 217 627 514 239 249 51 91 Cuts at: 2931 3113 3269 3275 3370 4825 618 Size: 182 156 6 95 1455 1842 Fragments arranged by size: 1842 1455 627 514 325 249 239 217 182 156 95 91 51 6 BanII G_rGCy'C Cuts at: 1391 2072 2690 3095 3260 3368 1391 Size: 681 618 405 165 108 4072 Fragments arranged by size: 4072 681 618 405 165 108 BbeI G_GCGC'C Cuts at: 2935 2935 Size: 6049 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5078 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4240 4274 1903 Size: 34 2303 34 3678 Fragments arranged by size: 3678 2303 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3941 5151 3941 Size: 1210 4839 BclI T'GATC_A Cuts at: 1548 1548 Size: 6049 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2288 2318 2929 Size: 107 122 71 631 605 616 30 611 Cuts at: 2929 4660 136 Size: 1731 1525 Fragments arranged by size: 1731 1525 631 616 611 605 122 107 71 30 BglII A'GATC_T Cuts at: 6044 6044 Size: 6049 BlpI GC'TnA_GC Cuts at: 3466 3466 Size: 6049 Bme1580I G_kGCm'C Cuts at: 1790 2279 2470 2522 2602 3116 4110 5266 1790 Size: 489 191 52 80 514 994 1156 2573 Fragments arranged by size: 2573 1156 994 514 489 191 80 52 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6049 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2309 2717 3780 Size: 416 53 83 399 24 892 408 1063 Cuts at: 3780 4738 442 Size: 958 1753 Fragments arranged by size: 1753 1063 958 892 416 408 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6049 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4709 1239 Size: 3470 2579 Bpu10I CC'TnA_GC Cuts at: 2411 2568 2689 3089 2411 Size: 157 121 400 5371 Fragments arranged by size: 5371 400 157 121 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4174 5039 5337 4174 Size: 865 298 4886 Fragments arranged by size: 4886 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2465 2648 4712 882 Size: 617 966 183 2064 2219 Fragments arranged by size: 2219 2064 966 617 183 BsaAI yAC'GTr Cuts at: 493 1204 2308 3307 3840 493 Size: 711 1104 999 533 2702 Fragments arranged by size: 2702 1104 999 711 533 BsaBI GATnn'nnATC Cuts at: 6043 6043 Size: 6049 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2932 3856 4238 4873 Size: 53 83 186 936 1399 924 382 635 Cuts at: 4873 275 Size: 1451 Fragments arranged by size: 1451 1399 936 924 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4482 4880 5154 5301 5712 4482 Size: 398 274 147 411 4819 Fragments arranged by size: 4819 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5824 Fragments arranged by size: 5824 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2741 1186 Size: 1555 4494 BseYI C'CCAG_C Cuts at: 2020 2173 2851 2959 3046 5252 5748 2020 Size: 153 678 108 87 2206 496 2321 Fragments arranged by size: 2321 2206 678 496 153 108 87 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2924 3031 1163 Size: 573 1188 107 4181 Fragments arranged by size: 4181 1188 573 107 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3221 3499 4260 5288 1540 Size: 275 186 1220 278 761 1028 2301 Fragments arranged by size: 2301 1220 1028 761 278 275 186 BsiHKAI G_wGCw'C Cuts at: 4110 4195 5266 4110 Size: 85 1071 4893 Fragments arranged by size: 4893 1071 85 BsmI GAATG_Cn' Cuts at: 3560 3653 3560 Size: 93 5956 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2072 2279 2470 2522 2602 2690 3095 Size: 399 282 207 191 52 80 88 405 Cuts at: 3095 3116 3260 3368 4110 4195 5266 1391 Size: 21 144 108 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 405 399 282 207 191 144 108 88 85 80 52 21 BspEI T'CCGG_A Cuts at: 5712 5712 Size: 6049 BspHI T'CATG_A Cuts at: 3938 5668 3938 Size: 1730 4319 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2052 2227 2427 3003 3404 844 Size: 1208 175 200 576 401 3489 Fragments arranged by size: 3489 1208 576 401 200 175 BsrBI CCG'CTC Cuts at: 1394 1823 3936 1394 Size: 429 2113 3507 Fragments arranged by size: 3507 2113 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2376 2759 3224 4544 4718 65 Size: 1183 1128 383 465 1320 174 1396 Fragments arranged by size: 1396 1320 1183 1128 465 383 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3220 3272 4693 4880 1700 Size: 110 1410 52 1421 187 2869 Fragments arranged by size: 2869 1421 1410 187 110 52 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6049 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6049 BssSI C'ACGA_G Cuts at: 4109 5121 4109 Size: 1012 5037 BstAPI GCAn_nnn'nTGC Cuts at: 794 2606 794 Size: 1812 4237 BstBI TT'CG_AA Cuts at: 4853 4853 Size: 6049 BstEII G'GTnAC_C Cuts at: 1184 2197 1184 Size: 1013 5036 BstXI CCAn_nnnn'nTGG Cuts at: 2136 2251 3253 2136 Size: 115 1002 4932 Fragments arranged by size: 4932 1002 115 BstYI r'GATC_y Cuts at: 1972 2091 2711 3379 3746 4131 4148 5600 5686 Size: 119 620 668 367 385 17 1452 86 Cuts at: 5686 5698 6044 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 668 620 385 367 346 119 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2811 2811 Size: 6049 BtgI C'CryG_G Cuts at: 513 1065 2004 2836 2997 513 Size: 552 939 832 161 3565 Fragments arranged by size: 3565 939 832 552 161 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5310 Fragments arranged by size: 5310 683 56 BtsI GCAGTG_nn' Cuts at: 2971 3117 3565 4359 4379 2971 Size: 146 448 794 20 4641 Fragments arranged by size: 4641 794 448 146 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6049 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6014 DraI TTT'AAA Cuts at: 3353 3353 Size: 6049 DraIII CAC_nnn'GTG Cuts at: 2308 2338 2308 Size: 30 6019 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2813 5056 809 Size: 507 1497 2243 1802 Fragments arranged by size: 2243 1802 1497 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2994 3218 3243 Size: 54 1425 50 275 18 1164 224 25 Cuts at: 3243 3496 4385 6014 8 Size: 253 889 1629 43 Fragments arranged by size: 1629 1425 1164 889 275 253 224 54 50 43 25 18 EagI C'GGCC_G Cuts at: 1537 1812 3218 3496 1537 Size: 275 1406 278 4090 Fragments arranged by size: 4090 1406 278 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2727 2742 3979 1353 Size: 1374 15 1237 3423 Fragments arranged by size: 3423 1374 1237 15 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4636 5010 5156 387 Size: 1064 138 3047 374 146 1280 Fragments arranged by size: 3047 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2607 4112 4709 5496 5764 1239 Size: 393 975 1505 597 787 268 1524 Fragments arranged by size: 1524 1505 975 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2129 2453 2129 Size: 324 5725 EcoO109I rG'GnC_Cy Cuts at: 2239 2693 3015 3063 2239 Size: 454 322 48 5225 Fragments arranged by size: 5225 454 322 48 EcoRI G'AATT_C Cuts at: 3358 3736 3358 Size: 378 5671 FspI TGC'GCA Cuts at: 4555 4555 Size: 6049 HaeII r_GCGC'y Cuts at: 1678 2174 2531 2935 5196 1678 Size: 496 357 404 2261 2531 Fragments arranged by size: 2531 2261 496 404 357 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4690 4722 4764 4796 1373 Size: 32 135 32 3118 32 42 32 2626 Fragments arranged by size: 3118 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3393 3639 669 Size: 988 281 1455 246 3079 Fragments arranged by size: 3079 1455 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6049 HpaI GTT'AAC Cuts at: 3639 3639 Size: 6049 KasI G'GCGC_C Cuts at: 2931 2931 Size: 6049 KpnI G_GTAC'C Cuts at: 3374 3374 Size: 6049 MfeI C'AATT_G Cuts at: 3648 3648 Size: 6049 MluI A'CGCG_T Cuts at: 5925 5925 Size: 6049 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2117 5163 5347 1305 Size: 812 3046 184 2007 Fragments arranged by size: 3046 2007 812 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2996 3245 10 Size: 54 1425 343 1164 249 2814 Fragments arranged by size: 2814 1425 1164 343 249 54 MslI CAynn'nnrTG Cuts at: 518 1166 2249 3002 3251 4007 4366 4525 518 Size: 648 1083 753 249 756 359 159 2042 Fragments arranged by size: 2042 1083 756 753 648 359 249 159 MspA1I CmG'CkG Cuts at: 1606 2627 2824 3236 3465 4142 5290 5535 1606 Size: 1021 197 412 229 677 1148 245 2120 Fragments arranged by size: 2120 1148 1021 677 412 245 229 197 NaeI GCC'GGC Cuts at: 3222 3222 Size: 6049 NarI GG'CG_CC Cuts at: 2932 2932 Size: 6049 NcoI C'CATG_G Cuts at: 513 1065 2004 2836 2997 513 Size: 552 939 832 161 3565 Fragments arranged by size: 3565 939 832 552 161 NdeI CA'TA_TG Cuts at: 387 3175 387 Size: 2788 3261 NgoMIV G'CCGG_C Cuts at: 3220 3220 Size: 6049 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6049 NotI GC'GGCC_GC Cuts at: 3496 3496 Size: 6049 NspI r_CATG'y Cuts at: 1132 1229 3055 3407 4952 1132 Size: 97 1826 352 1545 2229 Fragments arranged by size: 2229 1826 1545 352 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6049 PciI A'CATG_T Cuts at: 4948 4948 Size: 6049 PflMI CCAn_nnn'nTGG Cuts at: 1108 2047 2338 1108 Size: 939 291 4819 Fragments arranged by size: 4819 939 291 PmeI GTTT'AAAC Cuts at: 3353 3353 Size: 6049 PmlI CAC'GTG Cuts at: 2308 3307 2308 Size: 999 5050 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4118 4150 5631 5663 4118 Size: 32 1481 32 4504 Fragments arranged by size: 4504 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2239 3015 2239 Size: 776 5273 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6049 PsiI TTA'TAA Cuts at: 3619 3619 Size: 6049 PspXI vC'TCGA_Gb Cuts at: 2513 2513 Size: 6049 PstI C_TGCA'G Cuts at: 830 3124 3401 830 Size: 2294 277 3478 Fragments arranged by size: 3478 2294 277 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6049 PvuII CAG'CTG Cuts at: 2627 2824 3236 2627 Size: 197 412 5440 Fragments arranged by size: 5440 412 197 SalI G'TCGA_C Cuts at: 1936 3391 1936 Size: 1455 4594 SbfI CC_TGCA'GG Cuts at: 3401 3401 Size: 6049 ScaI AGT'ACT Cuts at: 4297 6009 4297 Size: 1712 4337 SfcI C'TryA_G Cuts at: 826 1045 3120 3397 4532 5213 5404 826 Size: 219 2075 277 1135 681 191 1471 Fragments arranged by size: 2075 1471 1135 681 277 219 191 SfoI GGC'GCC Cuts at: 2933 2933 Size: 6049 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6049 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6049 SmaI CCC'GGG Cuts at: 1872 2223 3376 1872 Size: 351 1153 4545 Fragments arranged by size: 4545 1153 351 SmlI C'TyrA_G Cuts at: 820 1017 1943 2513 4153 5054 5316 820 Size: 197 926 570 1640 901 262 1553 Fragments arranged by size: 1640 1553 926 901 570 262 197 SnaBI TAC'GTA Cuts at: 493 3840 493 Size: 3347 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6049 SphI G_CATG'C Cuts at: 1132 3055 3407 1132 Size: 1923 352 3774 Fragments arranged by size: 3774 1923 352 SspI AAT'ATT Cuts at: 5 52 3973 5 Size: 47 3921 2081 Fragments arranged by size: 3921 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6049 StyI C'CwwG_G Cuts at: 513 1065 2004 2358 2836 2997 3488 513 Size: 552 939 354 478 161 491 3074 Fragments arranged by size: 3074 939 552 491 478 354 161 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2029 2086 3139 4076 4093 4246 4431 Size: 1255 449 57 1053 937 17 153 185 Cuts at: 4431 4876 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1053 937 449 445 185 153 57 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4295 6007 96 Size: 274 80 33 51 1443 2318 1712 138 Fragments arranged by size: 2318 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3069 3101 6009 6041 3069 Size: 32 2908 32 3077 Fragments arranged by size: 3077 2908 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6049 XbaI T'CTAG_A Cuts at: 3385 3385 Size: 6049 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3124 3124 Size: 6049 XhoI C'TCGA_G Cuts at: 1943 2513 1943 Size: 570 5479 XmaI C'CCGG_G Cuts at: 1870 2221 3374 1870 Size: 351 1153 4545 Fragments arranged by size: 4545 1153 351 XmnI GAAnn'nnTTC Cuts at: 4178 4178 Size: 6049 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3857 276 Size: 53 83 186 936 2323 2468 Fragments arranged by size: 2468 2323 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PspOMI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI