Restriction map for
pFN21AE9540
(Circular) (Six-base) MAPSORT of: pFN21AE9540.seq Check: 8964 from: 1 to: 5995
pFN21AE9540 5995 bp
With 182 enzymes: SgfI *
December 28, 2011 15:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3273 3273
Size: 5995
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3805 278
Size: 53 83 186 936 2269 2468
Fragments arranged by size:
2468 2269 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2649 2928 3338 1937
Size: 712 279 410 4594
Fragments arranged by size:
4594 712 410 279
Acc65I G'GTAC_C
Cuts at: 3316 3316
Size: 5995
AclI AA'CG_TT
Cuts at: 1563 4122 4495 1563
Size: 2559 373 3063
Fragments arranged by size:
3063 2559 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4058 5442 5710 1632
Size: 2426 1384 268 1917
Fragments arranged by size:
2426 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5798
AflIII A'CryG_T
Cuts at: 1203 2241 4894 5871 1203
Size: 1038 2653 977 1327
Fragments arranged by size:
2653 1327 1038 977
AgeI A'CCGG_T
Cuts at: 4826 4826
Size: 5995
AhdI GACnn_n'nnGTC
Cuts at: 4724 4724
Size: 5995
AleI CACnn'nnGTG
Cuts at: 1166 2306 2405 2657 1166
Size: 1140 99 252 4504
Fragments arranged by size:
4504 1140 252 99
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5995
AlwNI CAG_nnn'CTG
Cuts at: 3738 5310 3738
Size: 1572 4423
ApaI G_GGCC'C
Cuts at: 2506 2750 3170 3184 2506
Size: 244 420 14 5317
Fragments arranged by size:
5317 420 244 14
ApaLI G'TGCA_C
Cuts at: 2441 2810 4052 5208 2441
Size: 369 1242 1156 3228
Fragments arranged by size:
3228 1242 1156 369
ApoI r'AATT_y
Cuts at: 1493 3304 3530 3682 5791 1493
Size: 1811 226 152 2109 1697
Fragments arranged by size:
2109 1811 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4549 160
Size: 872 3517 1606
Fragments arranged by size:
3517 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5995
AvaI C'yCGr_G
Cuts at: 1870 1943 2627 2743 2756 3320 1870
Size: 73 684 116 13 564 4545
Fragments arranged by size:
4545 684 564 116 73 13
BamHI G'GATC_C
Cuts at: 3325 3692 3325
Size: 367 5628
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2683 3316 4771 618
Size: 325 217 627 896 633 1455 1842
Fragments arranged by size:
1842 1455 896 633 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2019 2506 2750 3170 3184 3314 1391
Size: 628 487 244 420 14 130 4072
Fragments arranged by size:
4072 628 487 420 244 130 14
BbeI G_GCGC'C
Cuts at: 2687 2687
Size: 5995
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3112 928
Size: 971 1213 3811
Fragments arranged by size:
3811 1213 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2753 2787 4186 4220 1903
Size: 34 816 34 1399 34 3678
Fragments arranged by size:
3678 1399 816 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3887 5097 3887
Size: 1210 4785
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5995
BfrBI ATG'CAT
Cuts at: 2227 2227
Size: 5995
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2081 3100 4606
Size: 107 122 71 631 605 409 1019 1506
Cuts at: 4606 136
Size: 1525
Fragments arranged by size:
1525 1506 1019 631 605 409 122 107
71
BglII A'GATC_T
Cuts at: 5990 5990
Size: 5995
BlpI GC'TnA_GC
Cuts at: 2897 3412 2897
Size: 515 5480
Bme1580I G_kGCm'C
Cuts at: 1790 2445 2506 2750 2814 3170 3184 4056 5212
Size: 655 61 244 64 356 14 872 1156
Cuts at: 5212 1790
Size: 2573
Fragments arranged by size:
2573 1156 872 655 356 244 64 61
14
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5995
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2334 3726 4684
Size: 416 53 83 399 24 917 1392 958
Cuts at: 4684 442
Size: 1753
Fragments arranged by size:
1753 1392 958 917 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5995
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3100 3100
Size: 5995
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4655 1239
Size: 3416 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2195 4120 4985 5283 2195
Size: 1925 865 298 2907
Fragments arranged by size:
2907 1925 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4658 882
Size: 617 3159 2219
Fragments arranged by size:
3159 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3786 493
Size: 711 2582 2702
Fragments arranged by size:
2702 2582 711
BsaBI GATnn'nnATC
Cuts at: 5989 5989
Size: 5995
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2580 2684 3802 4184
Size: 53 83 186 936 1047 104 1118 382
Cuts at: 4184 4819 275
Size: 635 1451
Fragments arranged by size:
1451 1118 1047 936 635 382 186 104
83 53
BsaWI w'CCGG_w
Cuts at: 4428 4826 5100 5247 5658 4428
Size: 398 274 147 411 4765
Fragments arranged by size:
4765 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5770
Fragments arranged by size:
5770 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2092 2573 2780 1186
Size: 906 481 207 4401
Fragments arranged by size:
4401 906 481 207
BseYI C'CCAG_C
Cuts at: 2022 2613 3137 5198 5694 2022
Size: 591 524 2061 496 2323
Fragments arranged by size:
2323 2061 591 524 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2390 2897 3092 1163
Size: 573 654 507 195 4066
Fragments arranged by size:
4066 654 573 507 195
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2535 3196 3445 4206 5234 1540
Size: 275 186 534 661 249 761 1028 2301
Fragments arranged by size:
2301 1028 761 661 534 275 249 186
BsiHKAI G_wGCw'C
Cuts at: 2211 2445 2565 2664 2814 4056 4141 5212 2211
Size: 234 120 99 150 1242 85 1071 2994
Fragments arranged by size:
2994 1242 1071 234 150 120 99 85
BsmI GAATG_Cn'
Cuts at: 3506 3599 3506
Size: 93 5902
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2019 2211 2445 2506 2565 2664 2750
Size: 399 229 192 234 61 59 99 86
Cuts at: 2750 2814 3170 3184 3314 4056 4141 5212 1391
Size: 64 356 14 130 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 356 234 229 192
130 99 86 85 64 61 59 14
BspEI T'CCGG_A
Cuts at: 5658 5658
Size: 5995
BspHI T'CATG_A
Cuts at: 3884 5614 3884
Size: 1730 4265
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2280 3273 3350 844
Size: 1436 993 77 3489
Fragments arranged by size:
3489 1436 993 77
BsrBI CCG'CTC
Cuts at: 1394 1823 2361 2592 2602 2860 3091 3882 1394
Size: 429 538 231 10 258 231 791 3507
Fragments arranged by size:
3507 791 538 429 258 231 231 10
BsrDI GCAATG_nn'
Cuts at: 65 1248 2412 4490 4664 65
Size: 1183 1164 2078 174 1396
Fragments arranged by size:
2078 1396 1183 1164 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2217 4639 4826 1700
Size: 110 407 2422 187 2869
Fragments arranged by size:
2869 2422 407 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5995
BssHII G'CGCG_C
Cuts at: 1925 2723 2801 2901 1925
Size: 798 78 100 5019
Fragments arranged by size:
5019 798 100 78
BssSI C'ACGA_G
Cuts at: 4055 5067 4055
Size: 1012 4983
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2289 2733 794
Size: 1495 444 4056
Fragments arranged by size:
4056 1495 444
BstBI TT'CG_AA
Cuts at: 4799 4799
Size: 5995
BstEII G'GTnAC_C
Cuts at: 1184 2920 1184
Size: 1736 4259
BstXI CCAn_nnnn'nTGG
Cuts at: 2308 2419 2308
Size: 111 5884
BstYI r'GATC_y
Cuts at: 1972 3325 3692 4077 4094 5546 5632 5644 5990
Size: 1353 367 385 17 1452 86 12 346
Cuts at: 5990 1972
Size: 1977
Fragments arranged by size:
1977 1452 1353 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3016 3229 513
Size: 552 939 1012 213 3279
Fragments arranged by size:
3279 1012 939 552 213
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2473 2704 2772 487
Size: 683 56 1247 231 68 3710
Fragments arranged by size:
3710 1247 683 231 68 56
BtsI GCAGTG_nn'
Cuts at: 3181 3511 4305 4325 3181
Size: 330 794 20 4851
Fragments arranged by size:
4851 794 330 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5995
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2287 2322 529
Size: 35 1723 35 4202
Fragments arranged by size:
4202 1723 35 35
DraI TTT'AAA
Cuts at: 3299 3299
Size: 5995
DraIII CAC_nnn'GTG
Cuts at: 2328 2328
Size: 5995
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5002 809
Size: 507 3686 1802
Fragments arranged by size:
3686 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2133 2409 2532
Size: 54 1425 50 275 18 303 276 123
Cuts at: 2532 3193 3442 4331 5960 8
Size: 661 249 889 1629 43
Fragments arranged by size:
1629 1425 889 661 303 276 275 249
123 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2532 3193 3442 1537
Size: 275 720 661 249 4090
Fragments arranged by size:
4090 720 661 275 249
EarI CTCTTCn'nnn_
Cuts at: 1353 2669 3925 1353
Size: 1316 1256 3423
Fragments arranged by size:
3423 1316 1256
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3002 4582 4956 5102 387
Size: 1064 138 1413 1580 374 146 1280
Fragments arranged by size:
1580 1413 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 4058 4655 5442 5710 1239
Size: 393 2426 597 787 268 1524
Fragments arranged by size:
2426 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2471 2471
Size: 5995
EcoO109I rG'GnC_Cy
Cuts at: 2503 2740 3167 3180 3181 3248 2503
Size: 237 427 13 1 67 5250
Fragments arranged by size:
5250 427 237 67 13 1
EcoRI G'AATT_C
Cuts at: 3304 3682 3304
Size: 378 5617
EcoRV GAT'ATC
Cuts at: 3292 3292
Size: 5995
FspI TGC'GCA
Cuts at: 2642 4501 2642
Size: 1859 4136
HaeII r_GCGC'y
Cuts at: 1678 2480 2513 2687 2696 2834 2870 2976 5142
Size: 802 33 174 9 138 36 106 2166
Cuts at: 5142 1678
Size: 2531
Fragments arranged by size:
2531 2166 802 174 138 106 36 33
9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4636 4668 4710 4742 1373
Size: 32 135 32 3064 32 42 32 2626
Fragments arranged by size:
3064 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3339 3585 669
Size: 988 281 1401 246 3079
Fragments arranged by size:
3079 1401 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5995
HpaI GTT'AAC
Cuts at: 3585 3585
Size: 5995
KasI G'GCGC_C
Cuts at: 2683 2683
Size: 5995
KpnI G_GTAC'C
Cuts at: 3320 3320
Size: 5995
MfeI C'AATT_G
Cuts at: 3594 3594
Size: 5995
MluI A'CGCG_T
Cuts at: 5871 5871
Size: 5995
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5109 5293 1305
Size: 3804 184 2007
Fragments arranged by size:
3804 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2411 10
Size: 54 1425 343 579 3594
Fragments arranged by size:
3594 1425 579 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2240 2306 2381 2405 2417 2657 3953
Size: 648 1074 66 75 24 12 240 1296
Cuts at: 3953 4312 4471 518
Size: 359 159 2042
Fragments arranged by size:
2042 1296 1074 648 359 240 159 75
66 24 12
MspA1I CmG'CkG
Cuts at: 1606 2433 2689 2911 3018 3205 3411 4088 5236
Size: 827 256 222 107 187 206 677 1148
Cuts at: 5236 5481 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 827 677 256 245 222 206
187 107
NarI GG'CG_CC
Cuts at: 2684 2684
Size: 5995
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4504
Fragments arranged by size:
4504 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5995
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5995
NotI GC'GGCC_GC
Cuts at: 3442 3442
Size: 5995
NsiI A_TGCA'T
Cuts at: 2229 2229
Size: 5995
NspI r_CATG'y
Cuts at: 1132 1229 2227 2245 2296 2352 2380 2641 2818
Size: 97 998 18 51 56 28 261 177
Cuts at: 2818 3353 4898 1132
Size: 535 1545 2229
Fragments arranged by size:
2229 1545 998 535 261 177 97 56
51 28 18
PasI CC'CwG_GG
Cuts at: 1348 3240 1348
Size: 1892 4103
PciI A'CATG_T
Cuts at: 2241 4894 2241
Size: 2653 3342
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2572 1108
Size: 1464 4531
PmeI GTTT'AAAC
Cuts at: 3299 3299
Size: 5995
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4064 4096 5577 5609 4064
Size: 32 1481 32 4450
Fragments arranged by size:
4450 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5995
PsiI TTA'TAA
Cuts at: 3565 3565
Size: 5995
PspOMI G'GGCC_C
Cuts at: 2502 2746 3166 3180 2502
Size: 244 420 14 5317
Fragments arranged by size:
5317 420 244 14
PstI C_TGCA'G
Cuts at: 830 3347 830
Size: 2517 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5995
PvuII CAG'CTG
Cuts at: 2911 2911
Size: 5995
SacII CC_GC'GG
Cuts at: 3019 3019
Size: 5995
SalI G'TCGA_C
Cuts at: 1936 3337 1936
Size: 1401 4594
SapI GCTCTTCn'nnn_
Cuts at: 2669 2669
Size: 5995
SbfI CC_TGCA'GG
Cuts at: 3347 3347
Size: 5995
ScaI AGT'ACT
Cuts at: 4243 5955 4243
Size: 1712 4283
SexAI A'CCwGG_T
Cuts at: 2916 2916
Size: 5995
SfcI C'TryA_G
Cuts at: 826 1045 3343 4478 5159 5350 826
Size: 219 2298 1135 681 191 1471
Fragments arranged by size:
2298 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2685 2685
Size: 5995
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5995
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5995
SmaI CCC'GGG
Cuts at: 1872 2745 2758 3322 1872
Size: 873 13 564 4545
Fragments arranged by size:
4545 873 564 13
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2210 4099 5000 5262 820
Size: 197 926 267 1889 901 262 1553
Fragments arranged by size:
1889 1553 926 901 267 262 197
SnaBI TAC'GTA
Cuts at: 493 3786 493
Size: 3293 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5995
SphI G_CATG'C
Cuts at: 1132 2296 2641 3353 1132
Size: 1164 345 712 3774
Fragments arranged by size:
3774 1164 712 345
SspI AAT'ATT
Cuts at: 5 52 3919 5
Size: 47 3867 2081
Fragments arranged by size:
3867 2081 47
StuI AGG'CCT
Cuts at: 1517 2621 3080 1517
Size: 1104 459 4432
Fragments arranged by size:
4432 1104 459
StyI C'CwwG_G
Cuts at: 513 1065 2004 3434 513
Size: 552 939 1430 3074
Fragments arranged by size:
3074 1430 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2316 4022 4039 4192 4377 4822 325
Size: 1255 736 1706 17 153 185 445 1498
Fragments arranged by size:
1706 1498 1255 736 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4241 5953 96
Size: 274 80 33 51 1443 2264 1712 138
Fragments arranged by size:
2264 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5955 5987 5955
Size: 32 5963
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5995
XbaI T'CTAG_A
Cuts at: 3331 3331
Size: 5995
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2143 2305 2143
Size: 162 5833
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5995
XmaI C'CCGG_G
Cuts at: 1870 2743 2756 3320 1870
Size: 873 13 564 4545
Fragments arranged by size:
4545 873 564 13
XmnI GAAnn'nnTTC
Cuts at: 4124 4124
Size: 5995
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3803 276
Size: 53 83 186 936 2269 2468
Fragments arranged by size:
2468 2269 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI PvuII SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI Bpu10I BsiWI
BsmBI BstZ17I Bsu36I EcoICRI FalI FseI FspAI NaeI
NgoMIV NruI PacI PfoI PmlI PpuMI PspXI PsrI
RsrII SacI SanDI SfiI SrfI SwaI