Restriction map for
pFN21AE9538
(Circular) (Six-base) MAPSORT of: pFN21AE9538.seq Check: 9236 from: 1 to: 5689
pFN21AE9538 5689 bp
With 182 enzymes: SgfI *
March 28, 2012 12:01 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3499 278
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3032 1937
Size: 1095 4594
Acc65I G'GTAC_C
Cuts at: 3010 3010
Size: 5689
AclI AA'CG_TT
Cuts at: 1563 3816 4189 1563
Size: 2253 373 3063
Fragments arranged by size:
3063 2253 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2168 2521 2635 2813 3752 5136 5404 1632
Size: 536 353 114 178 939 1384 268 1917
Fragments arranged by size:
1917 1384 939 536 353 268 178 114
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5492
AflIII A'CryG_T
Cuts at: 1203 4588 5565 1203
Size: 3385 977 1327
Fragments arranged by size:
3385 1327 977
AgeI A'CCGG_T
Cuts at: 4520 4520
Size: 5689
AhdI GACnn_n'nnGTC
Cuts at: 4418 4418
Size: 5689
AleI CACnn'nnGTG
Cuts at: 1166 2617 1166
Size: 1451 4238
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5689
AlwNI CAG_nnn'CTG
Cuts at: 3432 5004 3432
Size: 1572 4117
ApaLI G'TGCA_C
Cuts at: 3746 4902 3746
Size: 1156 4533
ApoI r'AATT_y
Cuts at: 1493 2865 2998 3224 3376 5485 1493
Size: 1372 133 226 152 2109 1697
Fragments arranged by size:
2109 1697 1372 226 152 133
AseI AT'TA_AT
Cuts at: 160 1032 4243 160
Size: 872 3211 1606
Fragments arranged by size:
3211 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
AvaI C'yCGr_G
Cuts at: 1870 1943 3014 1870
Size: 73 1071 4545
Fragments arranged by size:
4545 1071 73
AvrII C'CTAG_G
Cuts at: 2860 2860
Size: 5689
BamHI G'GATC_C
Cuts at: 2496 3019 3386 2496
Size: 523 367 4799
Fragments arranged by size:
4799 523 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3010 4465 618
Size: 325 217 627 1223 1455 1842
Fragments arranged by size:
1842 1455 1223 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3008 1391
Size: 1617 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2801 928
Size: 971 902 3816
Fragments arranged by size:
3816 971 902
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3880 3914 1903
Size: 34 1943 34 3678
Fragments arranged by size:
3678 1943 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3581 4791 3581
Size: 1210 4479
BclI T'GATC_A
Cuts at: 1548 2406 1548
Size: 858 4831
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4300 136
Size: 107 122 71 631 605 2628 1525
Fragments arranged by size:
2628 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5684 5684
Size: 5689
BlpI GC'TnA_GC
Cuts at: 3106 3106
Size: 5689
Bme1580I G_kGCm'C
Cuts at: 1790 3750 4906 1790
Size: 1960 1156 2573
Fragments arranged by size:
2573 1960 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5689
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2906 3420 4378
Size: 416 53 83 399 24 1489 514 958
Cuts at: 4378 442
Size: 1753
Fragments arranged by size:
1753 1489 958 514 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5689
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2357 2357
Size: 5689
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2352 4349 1239
Size: 1113 1997 2579
Fragments arranged by size:
2579 1997 1113
Bpu10I CC'TnA_GC
Cuts at: 2257 2257
Size: 5689
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2209 3814 4679 4977 2209
Size: 1605 865 298 2921
Fragments arranged by size:
2921 1605 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4352 882
Size: 617 2853 2219
Fragments arranged by size:
2853 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3480 493
Size: 711 2276 2702
Fragments arranged by size:
2702 2276 711
BsaBI GATnn'nnATC
Cuts at: 5683 5683
Size: 5689
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3496 3878 4513 275
Size: 53 83 186 936 1963 382 635 1451
Fragments arranged by size:
1963 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4122 4520 4794 4941 5352 4122
Size: 398 274 147 411 4459
Fragments arranged by size:
4459 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5464
Fragments arranged by size:
5464 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5689
BseYI C'CCAG_C
Cuts at: 2386 4892 5388 2386
Size: 2506 496 2687
Fragments arranged by size:
2687 2506 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5116
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3139 3900 4928 1540
Size: 275 186 1138 761 1028 2301
Fragments arranged by size:
2301 1138 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2788 3750 3835 4906 2788
Size: 962 85 1071 3571
Fragments arranged by size:
3571 1071 962 85
BsmI GAATG_Cn'
Cuts at: 3200 3293 3200
Size: 93 5596
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2788 3008 3750 3835 4906 1391
Size: 399 998 220 742 85 1071 2174
Fragments arranged by size:
2174 1071 998 742 399 220 85
BspEI T'CCGG_A
Cuts at: 5352 5352
Size: 5689
BspHI T'CATG_A
Cuts at: 2394 3578 5308 2394
Size: 1184 1730 2775
Fragments arranged by size:
2775 1730 1184
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3044 844
Size: 2200 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2421 3576 1394
Size: 429 598 1155 3507
Fragments arranged by size:
3507 1155 598 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2701 4184 4358 65
Size: 1183 1453 1483 174 1396
Fragments arranged by size:
1483 1453 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4333 4520 1700
Size: 110 2523 187 2869
Fragments arranged by size:
2869 2523 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5689
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5689
BssSI C'ACGA_G
Cuts at: 2434 3749 4761 2434
Size: 1315 1012 3362
Fragments arranged by size:
3362 1315 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2015 794
Size: 1221 4468
BstBI TT'CG_AA
Cuts at: 4493 4493
Size: 5689
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5689
BstXI CCAn_nnnn'nTGG
Cuts at: 2269 2269
Size: 5689
BstYI r'GATC_y
Cuts at: 1972 2112 2496 3019 3386 3771 3788 5240 5326
Size: 140 384 523 367 385 17 1452 86
Cuts at: 5326 5338 5684 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 523 385 384 367 346 140
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2478 513
Size: 552 939 474 3724
Fragments arranged by size:
3724 939 552 474
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4950
Fragments arranged by size:
4950 683 56
BtsI GCAGTG_nn'
Cuts at: 2599 3205 3999 4019 2599
Size: 606 794 20 4269
Fragments arranged by size:
4269 794 606 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5689
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5654
DraI TTT'AAA
Cuts at: 2946 2993 2946
Size: 47 5642
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4696 809
Size: 507 3380 1802
Fragments arranged by size:
3380 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3136 4025 5654
Size: 54 1425 50 275 18 1306 889 1629
Cuts at: 5654 8
Size: 43
Fragments arranged by size:
1629 1425 1306 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3136 1537
Size: 275 1324 4090
Fragments arranged by size:
4090 1324 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2666 3619 1353
Size: 1313 953 3423
Fragments arranged by size:
3423 1313 953
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4276 4650 4796 387
Size: 1064 138 2687 374 146 1280
Fragments arranged by size:
2687 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2168 2352 2521 2635 2813 3752 4349
Size: 393 536 184 169 114 178 939 597
Cuts at: 4349 5136 5404 1239
Size: 787 268 1524
Fragments arranged by size:
1524 939 787 597 536 393 268 184
178 169 114
EcoNI CCTnn'n_nnAGG
Cuts at: 2732 2732
Size: 5689
EcoO109I rG'GnC_Cy
Cuts at: 2439 2725 2737 2439
Size: 286 12 5391
Fragments arranged by size:
5391 286 12
EcoRI G'AATT_C
Cuts at: 2998 3376 2998
Size: 378 5311
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2257 2257
Size: 5689
FspI TGC'GCA
Cuts at: 4195 4195
Size: 5689
HaeII r_GCGC'y
Cuts at: 1678 4836 1678
Size: 3158 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2533 2565 4330 4362 4404
Size: 32 135 32 961 32 1765 32 42
Cuts at: 4404 4436 1373
Size: 32 2626
Fragments arranged by size:
2626 1765 961 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3033 3279 669
Size: 988 281 1095 246 3079
Fragments arranged by size:
3079 1095 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2366 748
Size: 1618 4071
HpaI GTT'AAC
Cuts at: 3279 3279
Size: 5689
KpnI G_GTAC'C
Cuts at: 3014 3014
Size: 5689
MfeI C'AATT_G
Cuts at: 3288 3288
Size: 5689
MluI A'CGCG_T
Cuts at: 5565 5565
Size: 5689
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2250 2449 4803 4987 1305
Size: 945 199 2354 184 2007
Fragments arranged by size:
2354 2007 945 199 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3867
Fragments arranged by size:
3867 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2399 2617 3647 4006 4165 518
Size: 648 1233 218 1030 359 159 2042
Fragments arranged by size:
2042 1233 1030 648 359 218 159
MspA1I CmG'CkG
Cuts at: 1606 2655 3105 3782 4930 5175 1606
Size: 1049 450 677 1148 245 2120
Fragments arranged by size:
2120 1148 1049 677 450 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2478 513
Size: 552 939 474 3724
Fragments arranged by size:
3724 939 552 474
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5689
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5689
NotI GC'GGCC_GC
Cuts at: 3136 3136
Size: 5689
NspI r_CATG'y
Cuts at: 1132 1229 3047 4592 1132
Size: 97 1818 1545 2229
Fragments arranged by size:
2229 1818 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5689
PciI A'CATG_T
Cuts at: 4588 4588
Size: 5689
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2297 1108
Size: 1189 4500
PmeI GTTT'AAAC
Cuts at: 2993 2993
Size: 5689
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3758 3790 5271 5303 3758
Size: 32 1481 32 4144
Fragments arranged by size:
4144 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2725 2737 2725
Size: 12 5677
PshAI GACnn'nnGTC
Cuts at: 1613 2723 1613
Size: 1110 4579
PsiI TTA'TAA
Cuts at: 2637 3259 2637
Size: 622 5067
PstI C_TGCA'G
Cuts at: 830 2606 3041 830
Size: 1776 435 3478
Fragments arranged by size:
3478 1776 435
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5689
PvuII CAG'CTG
Cuts at: 2655 2655
Size: 5689
SalI G'TCGA_C
Cuts at: 1936 3031 1936
Size: 1095 4594
SbfI CC_TGCA'GG
Cuts at: 3041 3041
Size: 5689
ScaI AGT'ACT
Cuts at: 3937 5649 3937
Size: 1712 3977
SfcI C'TryA_G
Cuts at: 826 1045 2602 3037 4172 4853 5044 826
Size: 219 1557 435 1135 681 191 1471
Fragments arranged by size:
1557 1471 1135 681 435 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5689
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5689
SmaI CCC'GGG
Cuts at: 1872 3016 1872
Size: 1144 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2224 3793 4694 4956 820
Size: 197 926 281 1569 901 262 1553
Fragments arranged by size:
1569 1553 926 901 281 262 197
SnaBI TAC'GTA
Cuts at: 493 3480 493
Size: 2987 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5689
SphI G_CATG'C
Cuts at: 1132 3047 1132
Size: 1915 3774
SspI AAT'ATT
Cuts at: 5 52 3613 5
Size: 47 3561 2081
Fragments arranged by size:
3561 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5689
StyI C'CwwG_G
Cuts at: 513 1065 2004 2478 2860 3128 513
Size: 552 939 474 382 268 3074
Fragments arranged by size:
3074 939 552 474 382 268
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3716 3733 3886 4071 4516 325
Size: 1255 2136 17 153 185 445 1498
Fragments arranged by size:
2136 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3935 5647 96
Size: 274 80 33 51 1443 1958 1712 138
Fragments arranged by size:
1958 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2288 2320 5649 5681 2288
Size: 32 3329 32 2296
Fragments arranged by size:
3329 2296 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2101 1316
Size: 785 4904
XbaI T'CTAG_A
Cuts at: 3025 3025
Size: 5689
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2327 2485 2327
Size: 158 5531
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5689
XmaI C'CCGG_G
Cuts at: 1870 3014 1870
Size: 1144 4545
XmnI GAAnn'nnTTC
Cuts at: 3818 3818
Size: 5689
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3497 276
Size: 53 83 186 936 1963 2468
Fragments arranged by size:
2468 1963 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI